Standard name
Human Ortholog
Description Protein required for nuclear migration; component of the mitochondria-ER-cortex-ancor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and mitochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.48 0.61 0.34 0.33 0.41 0.26 0.19 0.08 0.14 0.08 0.08 0.08 0.68 0.51 0.48 0.39 0.37 0.32 0.41 0.35 0.38 0.3 0.37 0.31
Bud 0.13 0.17 0.19 0.24 0.34 0.24 0.22 0.22 0.17 0.18 0.21 0.15 0.17 0.16 0.17 0.11 0.13 0.14 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.14 0.08 0.12 0.05 0.08
Bud Site 0.07 0.07 0.2 0.21 0.13 0.12 0.09 0.07 0.11 0.12 0.07 0.1 0.14 0.06 0.11 0 0 0.08 – – – – – –
Cell Periphery 0.14 0.16 0.06 0.09 0.08 0.1 0.09 0.05 0.08 0.05 0.05 0.09 0.13 0.27 0.21 0.15 0.1 0.11 0.28 0.19 0.17 0.34 0.47 0.46
Cytoplasm 0.15 0.11 0.18 0.15 0.13 0.18 0.16 0.19 0.14 0.11 0.09 0.12 0 0.15 0.19 0.15 0.13 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.07 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0 0
Mitochondria 0.06 0.23 0.18 0.37 0.27 0.4 0.45 0.5 0.67 0.71 0.76 0.74 0.06 0 0.11 0.05 0 0.07 0.05 0.11 0.13 0.1 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.22 0.1 0.11 0.24 0.21 0.15 0.15 0.1 0.1 0.14 0.09 0.09 0.25 0.17 0.15 0.12 0.11 0.17 0 0 0.09 0 0 0
SpindlePole 0.23 0.06 0.28 0.14 0.16 0.15 0.16 0.21 0.08 0.12 0.09 0.1 0.14 0.16 0.09 0.28 0.39 0.27 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 162 78 148 117 185 148 128 56 61 51 28 32 129 104 112 85 114 94 172 61 215 52 87 93
Bud 46 21 84 85 153 137 151 149 77 120 74 59 33 32 40 24 39 40 10 2 10 2 1 4
Bud Neck 0 1 19 5 4 3 5 5 0 0 1 0 1 0 1 0 12 0 32 24 47 20 10 25
Bud Site 24 9 89 74 60 68 63 48 50 80 26 37 26 13 27 9 13 24 – – – – – –
Cell Periphery 48 20 27 31 38 55 60 31 37 34 19 35 25 55 49 32 32 32 116 33 96 59 110 139
Cytoplasm 50 14 80 55 57 104 112 125 64 71 33 47 6 30 44 32 39 49 3 4 8 2 1 4
Endoplasmic Reticulum 8 11 7 5 10 8 15 5 11 7 5 9 3 4 4 11 12 17 29 6 13 5 4 8
Endosome 7 0 0 0 2 4 1 7 0 0 0 0 2 4 2 5 5 2 9 4 25 2 2 5
Golgi 5 0 0 1 1 0 0 0 0 0 0 0 1 0 0 15 29 11 1 0 4 0 0 2
Mitochondria 22 29 80 132 123 230 309 334 303 474 274 284 11 3 26 11 13 20 19 18 74 16 7 13
Nucleus 4 2 2 2 5 5 4 13 3 5 6 1 3 0 0 5 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 1 0 0 1 0 0 0
Nucleolus 1 0 1 1 2 2 1 2 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 76 13 48 85 95 85 101 64 44 94 34 35 48 35 36 27 33 49 13 3 48 2 2 3
SpindlePole 78 7 121 51 72 86 108 137 34 79 31 37 27 33 22 62 118 78 1 0 4 1 0 0
Vac/Vac Membrane 1 0 0 0 1 0 0 3 1 1 1 2 0 0 0 6 7 4 8 11 11 2 1 2
Unique Cell Count 341 127 440 356 454 573 682 665 450 663 360 383 189 202 235 220 306 294 421 177 568 173 234 306
Labelled Cell Count 532 205 706 644 808 935 1058 979 686 1017 533 580 315 313 363 324 467 421 421 177 568 173 234 306


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 6.0 4.8 4.8 5.1 4.6 4.3 4.3 3.8 3.7 3.6 3.5 6.0 6.2 5.4 9.1 9.7 9.7 6.4 6.8 7.1
Std Deviation (1e-4) 1.2 1.1 0.9 1.2 1.3 1.1 1.1 1.2 1.1 0.9 0.9 1.0 1.0 1.1 1.0 1.3 1.5 1.8 1.3 1.5 1.4
Intensity Change (Log2) – – – -0.02 0.08 -0.07 -0.18 -0.17 -0.35 -0.4 -0.41 -0.45 0.3 0.35 0.14 0.9 1.0 0.99 0.39 0.48 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 2.2 0 0 0 0 0 0 0 8.0 4.3 3.6 1.3 1.0 -0.5
Bud 0 4.9 0 0 0 0 0 0 0 -0.5 -1.0 -0.7 -2.7 -2.3 -1.9
Bud Neck 0 -3.2 0 0 0 0 0 0 0 -2.5 -3.0 -2.8 -3.1 -0.3 -3.6
Bud Site 0 -2.8 0 0 0 0 0 0 0 -1.9 -4.4 -2.9 -5.5 -6.2 -4.4
Cell Periphery 0 1.3 0 0 0 0 0 0 0 3.0 7.4 5.8 3.6 2.2 2.3
Cytoplasm -1.0 -2.3 0 -0.8 0.3 -1.6 -3.5 -3.6 -2.3 -5.0 -1.0 0.2 -1.2 -2.0 -0.5
Endoplasmic Reticulum 0 0.7 0 0 0 0 0 0 0 0 0 0 2.5 2.0 3.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 5.5 6.6 0
Mitochondria 0 3.2 0 0 0 0 0 0 0 -4.0 -5.9 -2.4 -4.6 -5.7 -4.4
Nucleus 0 0 0 0 2.1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 4.1 0 0 0 0 0 0 0 4.6 2.3 1.7 0.5 -0.1 2.3
SpindlePole 0 -4.2 0 0 0 0 0 0 0 -3.6 -3.1 -5.5 0.2 3.2 -0.3
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4435 5.8351 5.404 5.1995 5.2607 5.998 1.3998 2.7498 2.2529 2.59 2.0448 2.6983 6.7075 8.2146 7.3946 7.9048 7.3886 7.8054
Actin 0.2664 0.2029 0.2353 0.2706 0.2592 0.3227 0.318 0.3403 0.272 0.3175 0.4077 0.4183 0.2087 0.1732 0.2648 0.3538 0.4279 0.3397
Bud 0.0036 0.0069 0.0058 0.0033 0.013 0.0067 0.0065 0.0056 0.0055 0.0058 0.0066 0.0045 0.0031 0.0039 0.004 0.0028 0.004 0.0047
Bud Neck 0.0386 0.0184 0.1048 0.1833 0.1287 0.1249 0.0305 0.0149 0.0583 0.067 0.0295 0.0623 0.0352 0.0192 0.0829 0.1319 0.0818 0.1277
Bud Periphery 0.0223 0.0136 0.0243 0.0171 0.0763 0.042 0.0244 0.0131 0.0272 0.0606 0.0322 0.0303 0.0162 0.0105 0.0215 0.0128 0.0265 0.0364
Bud Site 0.194 0.4791 0.2706 0.106 0.0348 0.0436 0.1477 0.3715 0.1839 0.0282 0.0442 0.0172 0.2191 0.5365 0.2653 0.0926 0.0332 0.0269
Cell Periphery 0.1667 0.105 0.1608 0.2128 0.2052 0.2225 0.2168 0.0753 0.1992 0.1737 0.081 0.164 0.3058 0.1093 0.1984 0.2196 0.2586 0.2574
Cytoplasm 0.0432 0.0517 0.0396 0.0393 0.0256 0.0413 0.038 0.0159 0.034 0.0277 0.0552 0.0253 0.0356 0.0435 0.0302 0.0305 0.0418 0.0225
Cytoplasmic Foci 0.0246 0.0272 0.0301 0.0143 0.0415 0.0341 0.019 0.0225 0.0169 0.0143 0.0458 0.0297 0.0079 0.0284 0.0126 0.0162 0.0089 0.0208
Eisosomes 0.0535 0.0161 0.0343 0.0446 0.0346 0.0495 0.0936 0.0599 0.0764 0.1366 0.0685 0.0934 0.1177 0.0516 0.0796 0.0581 0.0795 0.1173
Endoplasmic Reticulum 0.0108 0.0009 0.0013 0.0025 0.0007 0.0039 0.006 0.0038 0.0043 0.0072 0.0058 0.0032 0.0018 0.0004 0.0017 0.0032 0.0017 0.0014
Endosome 0.0206 0.0049 0.0022 0.008 0.0042 0.0064 0.0115 0.0019 0.0059 0.0029 0.0092 0.0093 0.0006 0.0003 0.0012 0.0008 0.0018 0.0009
Golgi 0.0199 0.0086 0.0062 0.0165 0.0196 0.0126 0.0157 0.0074 0.0123 0.0128 0.0192 0.0176 0.0029 0.0017 0.0038 0.0071 0.0111 0.0055
Lipid Particles 0.0251 0.0054 0.0134 0.0328 0.0102 0.0082 0.0145 0.0075 0.0036 0.0164 0.0128 0.0238 0.006 0.0014 0.0038 0.0073 0.0091 0.0031
Mitochondria 0.0209 0.0044 0.0381 0.0088 0.12 0.0285 0.0265 0.0199 0.0423 0.0785 0.1115 0.0537 0.0153 0.0026 0.0085 0.0242 0.0044 0.0126
None 0.0048 0.011 0.001 0.0197 0.0012 0.0013 0.0034 0.0017 0.0026 0.0032 0.0039 0.0033 0.002 0.0009 0.0011 0.0016 0.0011 0.0016
Nuclear Periphery 0.0292 0.0002 0.0002 0.0004 0.0001 0.0044 0.0005 0.0003 0.0027 0.0004 0.004 0.0041 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001
Nucleolus 0.0012 0.0007 0.0006 0.0006 0.0004 0.0007 0.001 0.0004 0.0007 0.0008 0.0011 0.0005 0.0003 0.0004 0.0004 0.0005 0.0006 0.0004
Nucleus 0.006 0.0004 0.0004 0.0004 0.0003 0.0013 0.0008 0.0003 0.0009 0.0004 0.0017 0.0009 0.0003 0.0003 0.0003 0.0003 0.0003 0.0003
Peroxisomes 0.0334 0.0404 0.0278 0.0159 0.0218 0.0395 0.0196 0.0364 0.0472 0.0424 0.0532 0.0348 0.0192 0.0146 0.0179 0.0346 0.0048 0.0185
Punctate Nuclear 0.0045 0.0005 0.0003 0.0005 0.0003 0.0004 0.0005 0.0003 0.0003 0.0003 0.0005 0.0012 0.0002 0.0002 0.0002 0.0003 0.0002 0.0001
Vacuole 0.0071 0.0017 0.0026 0.002 0.0017 0.004 0.005 0.001 0.0034 0.0028 0.0034 0.0021 0.0019 0.0011 0.0015 0.0014 0.0023 0.0017
Vacuole Periphery 0.0036 0.0001 0.0003 0.0005 0.0005 0.0016 0.0005 0.0001 0.0007 0.0006 0.0031 0.0005 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.0657 99.2715 93.2778 81.0947 67.9137 69.6684 120.7806 119.5336 96.1816 89.3559
Translational Efficiency 0.2533 0.2636 0.2507 0.2314 0.2092 0.2808 0.2214 0.2008 0.2199 0.2231

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein required for nuclear migration; component of the mitochondria-ER-cortex-ancor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and mitochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex
Localization
Cell Percentages peroxisome (18%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Num1

Num1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Num1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available