Standard name
Human Ortholog
Description Specific assembly chaperone for ribosomal protein Rpl4p; binds to an evolutionarily conserved surface extension of nascent Rpl4p and chaperones Rpl4p until its assembly into the pre-ribosome; transcriptionally co-regulated with rRNA and ribosome biosynthesis genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.15 0.12 0.18 0.08 0.05 0 0.07 0.07 0 0 0 0.05 0.26 0.19 0.23 0.16 0.19 0.14 0.05 0 0.07 0 0.07 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.19 0.07 0.12 0.22 0.34 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.22 0.42 0.31 0.8 0.82 0.76 0.66 0.72 0.7 0.72 0.59 0.62 0.47 0.58 0.66 0.28 0.23 0.2 0.7 0.75 0.51 0.67 0.41 0.35
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0.12 0.07 0.07 0.08 0.11 0.09 0 0 0 0 0 0 0 0 0.06 0 0.06 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.81 0.7 0.64 0.1 0.12 0.12 0.1 0.12 0.16 0.09 0.1 0.13 0.41 0.32 0.13 0.65 0.65 0.71 0.11 0.12 0.22 0.15 0.31 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 1 0 0 0
Bud 0 0 0 0 0 1 3 1 0 4 3 0 0 0 0 0 0 0 5 8 6 4 9 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 1 1 1 3 18 6 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 2 0 0 1 0 1 1 0 0 1 0 1 1 2 0 0 0 0 0 0
Cytoplasm 31 26 59 19 21 17 37 39 11 9 15 19 51 45 58 55 76 36 12 9 30 4 14 9
Endoplasmic Reticulum 1 0 2 0 0 2 1 1 0 0 0 0 0 0 2 14 14 12 0 0 5 1 1 0
Endosome 1 2 1 8 7 10 9 19 4 3 1 0 1 2 4 0 2 2 2 1 11 2 6 25
Golgi 1 3 2 0 0 0 0 0 0 0 0 0 0 0 3 16 29 4 1 0 1 0 0 3
Mitochondria 1 0 4 0 0 31 109 40 40 117 135 104 0 0 1 1 5 0 2 0 4 1 2 1
Nucleus 45 95 100 196 381 315 376 427 234 376 233 250 93 137 164 99 92 51 160 186 205 115 82 93
Nuclear Periphery 0 0 0 4 0 1 12 6 6 4 5 4 0 0 0 0 0 0 3 0 6 0 0 5
Nucleolus 2 1 4 7 18 26 68 44 22 43 45 38 0 0 3 1 5 4 9 7 23 7 12 16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 1 3 0 1 1 1 0 1 0 12 0 0 0 0 0 1 3 1 8 4 4 10
Vac/Vac Membrane 166 158 204 25 58 48 54 74 53 49 38 54 81 76 32 225 264 177 24 30 90 26 61 86
Unique Cell Count 204 226 319 246 466 415 566 594 335 522 393 403 200 237 247 348 406 250 229 250 403 174 201 266
Labelled Cell Count 248 286 377 262 487 453 671 654 373 625 482 485 226 261 268 413 488 290 229 250 403 174 201 266


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 13.6 10.7 11.8 12.1 8.1 7.0 7.8 7.4 6.6 6.2 9.2 13.0 11.8 10.8 19.1 18.1 16.9 13.4 13.6 13.9
Std Deviation (1e-4) 1.6 2.3 2.0 4.9 8.0 2.6 2.7 4.0 1.7 1.5 1.5 13.2 2.4 2.2 3.1 4.7 3.9 4.8 2.5 2.5 2.2
Intensity Change (Log2) – – – 0.14 0.18 -0.4 -0.6 -0.45 -0.54 -0.69 -0.77 -0.21 0.29 0.14 0.02 0.83 0.76 0.66 0.33 0.35 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.4 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.7 -6.4 -6.3 -5.5 -5.6 -6.3 -8.7 -6.4 -5.9 1.9 0.1 1.5 -0.9 0.1 -1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.9 2.6 3.2
Endosome 0 0 0 0 2.8 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.2 4.3 0
Mitochondria 0 0 3.9 7.7 3.7 5.5 8.5 11.1 9.2 0 0 0 0 0 0
Nucleus 11.4 14.2 12.1 10.1 11.8 9.8 11.6 7.4 8.2 3.5 6.2 8.3 -0.8 -2.6 -2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 2.2 3.4 5.6 4.0 3.5 4.3 5.3 4.7 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 2.7 0 0 0 0 0 0
Vacuole -12.9 -15.0 -14.8 -17.1 -16.1 -12.6 -16.7 -15.2 -14.1 -5.2 -7.4 -12.2 0.2 0.3 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.4205 10.1229 11.3506 10.4725 10.8763 10.882 10.052 12.0696 11.1589 10.4644 10.3384 10.9546 7.2663 9.9901 8.5593 7.9245 9.5553 8.8289
Actin 0 0.0001 0 0 0 0.0001 0.0085 0.0001 0.0083 0.0012 0.0043 0.0001 0.0284 0 0.0211 0.0287 0 0
Bud 0.0001 0.0003 0 0.0001 0 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0002 0.001 0 0.0003 0.0002 0 0.0001
Bud Neck 0.0003 0.0006 0.0002 0.0006 0.0011 0.0016 0.0025 0.0002 0.0045 0.0009 0.0004 0.0012 0.001 0.0001 0.0011 0.0012 0.0002 0.0007
Bud Periphery 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0004 0 0.0004 0.0001 0.0001 0.0002 0.002 0 0.0003 0.0001 0 0
Bud Site 0.0001 0.0009 0.0001 0.0003 0.0001 0.0003 0.0008 0.0002 0.0028 0.0002 0.0002 0.0001 0.0011 0.0001 0.0014 0.0005 0 0
Cell Periphery 0 0.0001 0 0 0 0 0.0001 0.0001 0.0002 0 0.0001 0 0.0003 0 0.0001 0.0001 0 0
Cytoplasm 0.0025 0.051 0.0069 0.0126 0.0006 0.0026 0.0077 0.0123 0.0109 0.0085 0.002 0.0031 0.0186 0.0173 0.0332 0.0116 0.0002 0.0014
Cytoplasmic Foci 0 0.002 0.0001 0.001 0 0.0007 0.0076 0 0.0032 0.0018 0.007 0.0002 0.0084 0 0.0022 0.0184 0 0
Eisosomes 0 0 0 0 0 0 0 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0004 0.0011 0.0001 0.0034 0.0001 0.0003 0.0043 0.0002 0.0026 0.0014 0.0006 0.0006 0.009 0.0001 0.0026 0.0031 0 0
Endosome 0.0005 0.0061 0.0002 0.0052 0.0001 0.0006 0.0143 0.0002 0.0061 0.004 0.017 0.0005 0.0221 0 0.0094 0.0066 0 0
Golgi 0 0.0001 0 0.0001 0 0.0001 0.0021 0 0.0014 0.0007 0.0028 0 0.0036 0 0.0015 0.0019 0 0
Lipid Particles 0 0.0001 0 0.0002 0 0.0003 0.0081 0 0.0008 0.0033 0.008 0.0001 0.0032 0 0.0019 0.0072 0 0
Mitochondria 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001 0.0024 0.0003 0.0017 0.0005 0.0183 0.0002 0.0062 0.0002 0.0015 0.0011 0.0002 0.0002
None 0 0.0053 0 0.0003 0 0.0002 0.0013 0.0001 0.0019 0.0005 0.0013 0.0001 0.007 0 0.0038 0.0138 0 0
Nuclear Periphery 0.0055 0.0201 0.0036 0.0068 0.0026 0.0056 0.0404 0.0082 0.0089 0.026 0.0051 0.0061 0.0321 0.0051 0.0188 0.0105 0.0017 0.0028
Nucleolus 0.0091 0.0139 0.0107 0.0079 0.0213 0.0296 0.0072 0.0081 0.0063 0.0064 0.0055 0.0168 0.0119 0.0119 0.0062 0.0121 0.0123 0.0098
Nucleus 0.9755 0.8759 0.9766 0.9519 0.9722 0.9459 0.8808 0.968 0.932 0.927 0.9197 0.966 0.8057 0.9639 0.884 0.8589 0.9851 0.9839
Peroxisomes 0 0.0001 0 0 0 0 0.0014 0 0.0008 0.0003 0.0013 0 0.002 0 0.0007 0.0031 0 0
Punctate Nuclear 0.002 0.0085 0.0009 0.0029 0.0012 0.0108 0.0045 0.0006 0.005 0.0155 0.0041 0.0035 0.0216 0.0008 0.0081 0.0167 0.0001 0.0006
Vacuole 0.0028 0.012 0.0004 0.0061 0.0004 0.0007 0.0028 0.001 0.0012 0.001 0.0007 0.0007 0.0106 0.0003 0.0009 0.0024 0.0001 0.0002
Vacuole Periphery 0.0008 0.0014 0.0002 0.0003 0.0001 0.0002 0.0024 0.0003 0.0007 0.0006 0.001 0.0002 0.0042 0.0001 0.0008 0.0016 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 52.6108 55.0811 54.7064 46.5422 60.2234 49.2657 45.8392 40.7693 43.1662 51.5061
Translational Efficiency 2.3594 1.8024 1.6138 1.6724 1.5951 1.6196 1.5207 1.5496 1.2403 1.442

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Specific assembly chaperone for ribosomal protein Rpl4p; binds to an evolutionarily conserved surface extension of nascent Rpl4p and chaperones Rpl4p until its assembly into the pre-ribosome; transcriptionally co-regulated with rRNA and ribosome biosynthesis genes
Localization
Cell Percentages nucleus (40%), cytoplasm (4%), mixed (55%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Acl4

Acl4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Acl4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available