Standard name
Human Ortholog
Description Subunit (145 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 – – 0 0 0 – 0 0 0 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.05 – – 0 0 0.07 – 0.06 0.05 0.07 0 0.05 0 0.05 0.07 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.11 – – 0 0.2 0 – 0.1 0.08 0.06 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nucleus – 0.75 – – 0.86 0.8 0.71 – 0.7 0.67 0.67 0.73 0.78 0.82 0.83 0.76 0.75 0.7 0.86 0.76 0.77 0.7 0.61 0.53
Nuclear Periphery – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0.52 – – 0.32 0.43 0.34 – 0.54 0.54 0.62 0.64 0.4 0.37 0.32 0.28 0.29 0.23 0.12 0.15 0.22 0.23 0.29 0.35
Peroxisomes – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 2 3 6
Bud Neck – 1 – – 0 0 0 – 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 1
Bud Site – 0 – – 0 0 0 – 0 2 2 9 0 1 1 0 0 0 – – – – – –
Cell Periphery – 0 – – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 6 – – 1 0 10 – 4 4 9 4 10 19 21 13 10 12 0 0 0 0 0 1
Endoplasmic Reticulum – 0 – – 0 0 0 – 0 0 0 0 0 0 0 2 1 2 0 0 0 0 0 1
Endosome – 0 – – 0 0 1 – 0 0 0 0 0 3 0 0 2 1 0 1 0 1 3 5
Golgi – 0 – – 0 0 0 – 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 2
Mitochondria – 12 – – 0 7 5 – 7 6 7 1 6 14 9 8 7 7 0 0 0 0 1 2
Nucleus – 83 – – 49 28 96 – 47 51 80 77 153 383 357 143 111 100 21 85 13 167 229 229
Nuclear Periphery – 0 – – 0 1 1 – 0 0 0 0 0 4 2 0 2 0 0 0 0 0 0 0
Nucleolus – 57 – – 18 15 46 – 36 41 75 68 79 172 139 52 43 33 2 16 3 55 109 150
Peroxisomes – 0 – – 0 0 0 – 0 0 0 0 1 2 0 0 0 1 0 0 0 0 0 0
SpindlePole – 0 – – 0 0 0 – 0 2 0 0 0 4 4 0 0 2 0 2 0 0 1 6
Vac/Vac Membrane – 1 – – 0 1 0 – 0 0 0 0 1 3 3 7 7 5 0 3 0 10 23 22
Unique Cell Count 110 57 35 136 67 76 120 106 197 466 432 189 148 142 25 113 17 239 379 434
Labelled Cell Count 160 68 52 159 94 106 174 159 251 605 536 226 184 163 25 113 17 239 379 434


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 8.5 – – 9.4 6.7 8.9 – 7.5 7.9 8.1 8.5 10.7 10.1 10.9 13.4 12.1 15.4 9.6 9.5 9.5
Std Deviation (1e-4) – 2.2 – – 2.6 1.9 2.1 – 2.1 2.1 2.4 2.1 3.3 2.7 3.7 5.4 4.5 4.7 2.6 3.3 3.2
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9634 3.3927 2.7463 1.8107 1.518 2.2544 4.5091 5.6321 4.5786 4.4026 3.8097 5.3607 5.3226 6.4105 5.5026 5.5221 5.2803 5.6966
Actin 0.0099 0.0001 0.0013 0 0.0009 0 0.0241 0 0.0062 0 0.0417 0.0008 0.0001 0 0.0005 0.0005 0.0006 0
Bud 0.0003 0.0002 0.0005 0 0.0001 0.0001 0.0004 0 0.0005 0 0.001 0.0001 0.0013 0.0003 0.0001 0 0.0001 0.0001
Bud Neck 0.0003 0.0002 0.0005 0 0.0004 0.0011 0.0005 0 0.0043 0 0.0004 0.0006 0.0006 0.0004 0.0001 0.0001 0.0003 0.0009
Bud Periphery 0.0004 0.0003 0.0011 0 0.0004 0.0002 0.0004 0 0.0006 0 0.0018 0.0001 0.003 0.0005 0.0001 0 0.0003 0.0002
Bud Site 0.0013 0.0003 0.0008 0 0.0004 0.0002 0.0013 0 0.0008 0 0.0013 0.0002 0.001 0.0009 0.0001 0.0001 0.0003 0.0002
Cell Periphery 0.0001 0 0.0005 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0007 0 0 0 0 0.0001
Cytoplasm 0.0303 0.0004 0.0159 0.0001 0.0004 0.0002 0.0679 0 0.0011 0 0.0032 0.0001 0.003 0.0001 0.0043 0.0059 0.0163 0
Cytoplasmic Foci 0.0209 0.0002 0.0023 0 0.0014 0.0002 0.0125 0 0.0101 0 0.018 0.0019 0.0005 0.0003 0.0098 0.0002 0.0003 0
Eisosomes 0.0002 0 0 0 0.0001 0 0.0002 0 0 0 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0023 0.0002 0.0007 0 0.0006 0 0.0031 0 0.0009 0 0.0045 0 0.0003 0 0.0003 0.0005 0.0008 0
Endosome 0.0402 0.0022 0.0022 0 0.007 0.0002 0.0148 0 0.0152 0 0.0238 0.0002 0.0012 0.0001 0.0011 0.0004 0.0012 0
Golgi 0.018 0.0001 0.0005 0 0.0014 0 0.0044 0 0.0062 0 0.0105 0.0003 0.0001 0.0001 0.0003 0 0.0001 0
Lipid Particles 0.03 0.0001 0.0004 0 0.0098 0.0001 0.0103 0 0.0039 0 0.0137 0.0008 0.0002 0.0001 0.0009 0 0.0001 0
Mitochondria 0.0227 0.0014 0.0011 0.0001 0.0081 0.0002 0.0045 0.0002 0.008 0.0002 0.0234 0.0002 0.0009 0.001 0.0004 0.0002 0.0019 0.0002
None 0.03 0.0002 0.0019 0 0.0007 0.0001 0.0222 0 0.0004 0 0.0036 0.0001 0.0007 0.0001 0.005 0.0022 0.0091 0
Nuclear Periphery 0.0241 0.0033 0.0063 0.0002 0.0164 0.0008 0.0277 0.0001 0.0051 0.0002 0.067 0.0004 0.0108 0.0002 0.002 0.0057 0.0074 0.0002
Nucleolus 0.0538 0.0769 0.0574 0.0585 0.2874 0.2327 0.0954 0.0733 0.034 0.0354 0.0408 0.235 0.1001 0.0873 0.0583 0.0777 0.2063 0.1815
Nucleus 0.6354 0.9097 0.8808 0.9409 0.6357 0.7532 0.6721 0.9262 0.8956 0.964 0.6739 0.7278 0.8551 0.9065 0.9068 0.8943 0.7462 0.8159
Peroxisomes 0.0157 0 0.0005 0 0.001 0 0.0042 0 0.0022 0 0.0384 0.0003 0.0001 0.0001 0.0009 0 0.0001 0
Punctate Nuclear 0.0613 0.0008 0.0228 0.0001 0.0239 0.0102 0.0317 0.0001 0.0028 0.0001 0.0286 0.0307 0.0183 0.0017 0.0089 0.0119 0.0081 0.0003
Vacuole 0.0015 0.0021 0.0016 0 0.0015 0.0002 0.0011 0 0.0008 0 0.0009 0.0001 0.0015 0.0002 0.0001 0.0001 0.0003 0.0004
Vacuole Periphery 0.0014 0.0014 0.001 0 0.0019 0.0001 0.0012 0 0.0011 0 0.0031 0 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.3175 12.7069 24.7514 31.2878 29.9484 17.7584 29.7109 28.7244 28.0075 32.6847
Translational Efficiency 2.8334 6.0332 2.9431 2.3948 2.5186 3.7549 2.4734 2.3489 2.5772 1.8822

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit (145 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Taf10

Taf10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Taf10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available