Standard name | |
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Human Ortholog | |
Description | Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 2 | 4 | 7 | 9 | 14 | 17 | 15 | 18 | 17 | 30 | 13 | 18 | 12 | 15 | 17 | 20 | 3 | 0 | 4 | 5 | 17 | 19 |
Bud | 12 | 2 | 3 | 4 | 6 | 3 | 12 | 10 | 13 | 10 | 9 | 9 | 9 | 8 | 5 | 0 | 6 | 13 | 8 | 1 | 10 | 5 | 27 | 39 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 6 | 2 | 4 | 12 | 13 | 25 | 36 | 31 | 12 | 23 | 26 | 36 | 3 | 13 | 6 | 9 | 7 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 354 | 165 | 362 | 290 | 305 | 373 | 367 | 311 | 213 | 250 | 199 | 163 | 337 | 221 | 150 | 135 | 126 | 158 | 371 | 184 | 325 | 278 | 231 | 204 |
Endoplasmic Reticulum | 0 | 3 | 3 | 4 | 5 | 13 | 18 | 22 | 11 | 15 | 21 | 20 | 0 | 2 | 0 | 10 | 6 | 3 | 13 | 3 | 10 | 6 | 12 | 9 |
Endosome | 6 | 0 | 7 | 5 | 7 | 3 | 1 | 1 | 1 | 1 | 2 | 1 | 56 | 82 | 116 | 20 | 18 | 23 | 5 | 3 | 14 | 22 | 80 | 67 |
Golgi | 1 | 1 | 3 | 1 | 7 | 7 | 16 | 4 | 7 | 13 | 8 | 15 | 4 | 19 | 17 | 41 | 31 | 20 | 2 | 0 | 1 | 5 | 30 | 19 |
Mitochondria | 13 | 0 | 6 | 17 | 21 | 47 | 62 | 39 | 68 | 48 | 52 | 25 | 6 | 3 | 1 | 9 | 12 | 17 | 6 | 3 | 20 | 23 | 28 | 23 |
Nucleus | 2 | 2 | 2 | 0 | 0 | 3 | 2 | 3 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 3 | 1 | 0 | 0 | 0 | 2 | 5 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Nucleolus | 2 | 3 | 4 | 3 | 2 | 1 | 5 | 7 | 7 | 1 | 2 | 2 | 5 | 18 | 32 | 24 | 25 | 17 | 0 | 0 | 1 | 0 | 1 | 2 |
Peroxisomes | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 26 | 67 | 6 | 6 | 7 | 1 | 0 | 0 | 0 | 18 | 16 |
SpindlePole | 0 | 0 | 6 | 4 | 5 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 35 | 73 | 128 | 45 | 23 | 19 | 7 | 0 | 11 | 9 | 47 | 37 |
Vac/Vac Membrane | 6 | 3 | 26 | 31 | 53 | 74 | 144 | 140 | 102 | 114 | 53 | 104 | 22 | 36 | 24 | 60 | 39 | 33 | 12 | 4 | 9 | 6 | 11 | 10 |
Unique Cell Count | 382 | 176 | 408 | 338 | 387 | 509 | 599 | 531 | 404 | 432 | 330 | 342 | 451 | 415 | 430 | 291 | 246 | 274 | 444 | 209 | 423 | 373 | 521 | 466 |
Labelled Cell Count | 407 | 183 | 429 | 376 | 431 | 561 | 682 | 586 | 453 | 497 | 389 | 406 | 508 | 519 | 563 | 375 | 317 | 337 | 444 | 209 | 423 | 373 | 521 | 466 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 12.1 | 8.4 | 8.2 | 11.9 | 12.7 | 14.4 | 16.0 | 15.7 | 17.8 | 18.1 | 19.9 | 9.5 | 12.6 | 14.7 | 22.3 | 21.0 | 19.6 | 6.8 | 7.7 | 9.5 |
Std Deviation (1e-4) | 2.3 | 6.1 | 2.5 | 2.8 | 3.7 | 3.9 | 4.9 | 6.4 | 7.0 | 8.7 | 10.4 | 10.9 | 3.2 | 4.1 | 6.2 | 9.6 | 8.8 | 9.3 | 2.3 | 1.8 | 3.1 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.5 | 0.6 | 0.77 | 0.93 | 0.9 | 1.08 | 1.11 | 1.24 | 0.17 | 0.59 | 0.81 | 1.41 | 1.32 | 1.22 | -0.31 | -0.13 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 2.3 | 2.9 | 3.2 | 3.5 | 4.0 | 5.6 | 2.7 | 3.6 | 2.6 | 3.9 | 4.7 | 4.9 |
Bud | 0 | 0 | 0 | 1.6 | 1.5 | 2.5 | 1.9 | 2.1 | 2.1 | 1.6 | 1.5 | 0 | 0 | 0 | 3.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.4 | 2.3 | 3.4 | 4.0 | 3.9 | 2.0 | 3.6 | 4.7 | 5.8 | 0 | 2.2 | 0 | 2.0 | 0 | 0 |
Cytoplasm | -1.2 | -3.8 | -5.8 | -9.6 | -10.2 | -11.3 | -10.1 | -9.0 | -12.2 | -5.3 | -11.2 | -16.0 | -12.2 | -10.7 | -9.3 |
Endoplasmic Reticulum | 0 | 0 | 2.1 | 2.5 | 3.2 | 2.2 | 2.7 | 4.3 | 4.0 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Endosome | -0.3 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.0 | 8.3 | 10.3 | 3.5 | 3.6 | 4.2 |
Golgi | 0 | 0 | 0 | 2.2 | 0 | 0 | 2.4 | 0 | 3.3 | 0 | 3.4 | 3.1 | 7.2 | 6.6 | 4.7 |
Mitochondria | 2.8 | 3.1 | 5.0 | 5.5 | 4.2 | 7.6 | 5.7 | 7.2 | 4.0 | -0.2 | 0 | 0 | 1.5 | 2.6 | 3.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0.9 | 0 | 0 | 0 | 0 | 3.0 | 4.6 | 4.8 | 5.5 | 3.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 4.8 | 8.1 | 0 | 0 | 0 |
SpindlePole | 0 | -0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 7.8 | 11.2 | 7.0 | 4.7 | 3.7 |
Vacuole | 1.4 | 3.4 | 3.9 | 7.3 | 8.0 | 7.4 | 7.8 | 4.2 | 8.7 | -1.0 | 1.3 | -0.5 | 5.7 | 3.9 | 2.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.8603 | 9.0435 | 8.5599 | 7.7549 | 8.4784 | 8.1531 | 3.7643 | 5.2194 | 4.9187 | 4.1509 | 4.0208 | 4.4879 | 4.3917 | 5.6084 | 5.1914 | 4.6284 | 4.4775 | 5.0062 |
Actin | 0.022 | 0.0032 | 0.0045 | 0.0376 | 0.0164 | 0.0017 | 0.0126 | 0.009 | 0.0145 | 0.001 | 0.0001 | 0.0187 | 0.0418 | 0.0005 | 0.007 | 0.01 | 0.0492 | 0.0181 |
Bud | 0.0035 | 0.0059 | 0.0073 | 0.004 | 0.0095 | 0.001 | 0.0008 | 0.0028 | 0.0038 | 0.0007 | 0.0002 | 0.0008 | 0.0018 | 0.0007 | 0.0053 | 0.0003 | 0.0168 | 0.0042 |
Bud Neck | 0.0036 | 0.0004 | 0.001 | 0.0012 | 0.002 | 0.0016 | 0.0016 | 0.0005 | 0.0048 | 0.0035 | 0.0006 | 0.002 | 0.0075 | 0.0002 | 0.0014 | 0.0016 | 0.0086 | 0.002 |
Bud Periphery | 0.0029 | 0.0052 | 0.0043 | 0.004 | 0.0067 | 0.0006 | 0.0006 | 0.0014 | 0.0058 | 0.0001 | 0 | 0.0007 | 0.0017 | 0.0003 | 0.0021 | 0.0002 | 0.012 | 0.0051 |
Bud Site | 0.0059 | 0.0106 | 0.0072 | 0.0032 | 0.0044 | 0.0003 | 0.0048 | 0.0047 | 0.0025 | 0.0018 | 0.0003 | 0.0003 | 0.0317 | 0.0035 | 0.0109 | 0.0019 | 0.0042 | 0.0008 |
Cell Periphery | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0031 | 0.0002 |
Cytoplasm | 0.8 | 0.9103 | 0.8605 | 0.7278 | 0.7395 | 0.8655 | 0.7189 | 0.9206 | 0.8252 | 0.7125 | 0.8392 | 0.8532 | 0.6062 | 0.9305 | 0.9056 | 0.9065 | 0.7686 | 0.8632 |
Cytoplasmic Foci | 0.0324 | 0.0194 | 0.0318 | 0.0556 | 0.0404 | 0.0438 | 0.0569 | 0.0202 | 0.0203 | 0.0475 | 0.0996 | 0.035 | 0.0647 | 0.0121 | 0.0084 | 0.0143 | 0.0244 | 0.0188 |
Eisosomes | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0111 | 0.0105 | 0.0063 | 0.0036 | 0.0058 | 0.007 | 0.0097 | 0.004 | 0.0067 | 0.0044 | 0.0042 | 0.0156 | 0.0117 | 0.0046 | 0.0033 | 0.0054 | 0.0094 | 0.0029 |
Endosome | 0.043 | 0.0141 | 0.0276 | 0.0957 | 0.1028 | 0.0418 | 0.0475 | 0.0073 | 0.0261 | 0.0706 | 0.0364 | 0.0351 | 0.0735 | 0.0054 | 0.0169 | 0.019 | 0.0324 | 0.0447 |
Golgi | 0.0092 | 0.0016 | 0.0053 | 0.0148 | 0.0275 | 0.0038 | 0.014 | 0.0015 | 0.0152 | 0.007 | 0.0018 | 0.0053 | 0.0213 | 0.0005 | 0.0029 | 0.0231 | 0.0236 | 0.0066 |
Lipid Particles | 0.0035 | 0.0001 | 0.0015 | 0.0083 | 0.0074 | 0.0017 | 0.0181 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0041 | 0.009 | 0.0001 | 0.0033 | 0.0031 | 0.0049 | 0.0009 |
Mitochondria | 0.0045 | 0.0007 | 0.0041 | 0.0033 | 0.0131 | 0.002 | 0.0063 | 0.0008 | 0.0046 | 0.0011 | 0.0005 | 0.002 | 0.0086 | 0.0002 | 0.0078 | 0.0028 | 0.0033 | 0.0107 |
None | 0.0258 | 0.0075 | 0.0102 | 0.0034 | 0.0024 | 0.0128 | 0.0651 | 0.0203 | 0.0515 | 0.1393 | 0.0105 | 0.0128 | 0.0224 | 0.0355 | 0.0119 | 0.0048 | 0.0138 | 0.0041 |
Nuclear Periphery | 0.008 | 0.0018 | 0.0025 | 0.0023 | 0.0025 | 0.0027 | 0.0052 | 0.001 | 0.0066 | 0.0015 | 0.0009 | 0.0019 | 0.0336 | 0.0011 | 0.0016 | 0.0011 | 0.0068 | 0.002 |
Nucleolus | 0.0003 | 0.0001 | 0.004 | 0.0002 | 0.0008 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.002 | 0.0001 | 0.0007 | 0.0001 | 0.0006 | 0.0002 |
Nucleus | 0.0092 | 0.0034 | 0.0042 | 0.0039 | 0.004 | 0.0045 | 0.003 | 0.0022 | 0.0039 | 0.0017 | 0.0016 | 0.0024 | 0.0086 | 0.0016 | 0.0025 | 0.0018 | 0.0037 | 0.0017 |
Peroxisomes | 0.0026 | 0.0004 | 0.0073 | 0.0081 | 0.006 | 0.0017 | 0.0183 | 0.0008 | 0.0016 | 0.0016 | 0.0011 | 0.0015 | 0.0108 | 0.0003 | 0.0031 | 0.001 | 0.0035 | 0.0012 |
Punctate Nuclear | 0.0018 | 0.0007 | 0.0025 | 0.0104 | 0.0019 | 0.0019 | 0.0077 | 0.0006 | 0.0005 | 0.0005 | 0.0006 | 0.0037 | 0.0306 | 0.0004 | 0.0007 | 0.0005 | 0.0013 | 0.0012 |
Vacuole | 0.0079 | 0.0032 | 0.0066 | 0.0101 | 0.005 | 0.0041 | 0.0056 | 0.0016 | 0.0037 | 0.0039 | 0.0017 | 0.003 | 0.0068 | 0.002 | 0.003 | 0.0018 | 0.008 | 0.0072 |
Vacuole Periphery | 0.0023 | 0.0005 | 0.0013 | 0.002 | 0.0017 | 0.001 | 0.0016 | 0.0003 | 0.0014 | 0.0012 | 0.0006 | 0.0016 | 0.0048 | 0.0002 | 0.0011 | 0.0006 | 0.0019 | 0.0042 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.2056 | 16.6145 | 10.382 | 28.4299 | 19.6582 | 13.6872 | 25.4194 | 49.2313 | 50.9295 | 21.5304 |
Translational Efficiency | 0.8368 | 1.3693 | 1.1913 | 1.1667 | 1.0837 | 1.9276 | 1.2707 | 1.045 | 1.0879 | 0.9977 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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