Standard name
Human Ortholog
Description Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.08
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.05 0.06 0.06 0 0.05 0.08 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.94 0.89 0.86 0.79 0.73 0.61 0.59 0.53 0.58 0.6 0.48 0.75 0.53 0.35 0.46 0.51 0.58 0.84 0.88 0.77 0.75 0.44 0.44
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.2 0.27 0.07 0.07 0.08 0 0 0 0.06 0.16 0.14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.14 0.13 0.07 0 0 0 0 0.06 0
Mitochondria 0 0 0 0.05 0.05 0.09 0.1 0.07 0.17 0.11 0.16 0.07 0 0 0 0 0.05 0.06 0 0 0.05 0.06 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.1 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.16 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.18 0.3 0.15 0.09 0.07 0 0 0 0 0.09 0.08
Vac/Vac Membrane 0 0 0.06 0.09 0.14 0.15 0.24 0.26 0.25 0.26 0.16 0.3 0.05 0.09 0.06 0.21 0.16 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 2 4 7 9 14 17 15 18 17 30 13 18 12 15 17 20 3 0 4 5 17 19
Bud 12 2 3 4 6 3 12 10 13 10 9 9 9 8 5 0 6 13 8 1 10 5 27 39
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 0 0 0 1 1 2 1 2 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Cell Periphery 6 2 4 12 13 25 36 31 12 23 26 36 3 13 6 9 7 6 0 0 0 0 0 0
Cytoplasm 354 165 362 290 305 373 367 311 213 250 199 163 337 221 150 135 126 158 371 184 325 278 231 204
Endoplasmic Reticulum 0 3 3 4 5 13 18 22 11 15 21 20 0 2 0 10 6 3 13 3 10 6 12 9
Endosome 6 0 7 5 7 3 1 1 1 1 2 1 56 82 116 20 18 23 5 3 14 22 80 67
Golgi 1 1 3 1 7 7 16 4 7 13 8 15 4 19 17 41 31 20 2 0 1 5 30 19
Mitochondria 13 0 6 17 21 47 62 39 68 48 52 25 6 3 1 9 12 17 6 3 20 23 28 23
Nucleus 2 2 2 0 0 3 2 3 4 1 0 1 0 0 0 1 0 0 0 1 0 0 0 0
Nuclear Periphery 3 1 0 0 0 2 5 0 0 3 0 0 1 0 0 0 0 0 0 0 0 1 1 2
Nucleolus 2 3 4 3 2 1 5 7 7 1 2 2 5 18 32 24 25 17 0 0 1 0 1 2
Peroxisomes 0 0 1 1 0 0 0 0 0 0 0 0 17 26 67 6 6 7 1 0 0 0 18 16
SpindlePole 0 0 6 4 5 1 0 1 0 0 0 0 35 73 128 45 23 19 7 0 11 9 47 37
Vac/Vac Membrane 6 3 26 31 53 74 144 140 102 114 53 104 22 36 24 60 39 33 12 4 9 6 11 10
Unique Cell Count 382 176 408 338 387 509 599 531 404 432 330 342 451 415 430 291 246 274 444 209 423 373 521 466
Labelled Cell Count 407 183 429 376 431 561 682 586 453 497 389 406 508 519 563 375 317 337 444 209 423 373 521 466


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 12.1 8.4 8.2 11.9 12.7 14.4 16.0 15.7 17.8 18.1 19.9 9.5 12.6 14.7 22.3 21.0 19.6 6.8 7.7 9.5
Std Deviation (1e-4) 2.3 6.1 2.5 2.8 3.7 3.9 4.9 6.4 7.0 8.7 10.4 10.9 3.2 4.1 6.2 9.6 8.8 9.3 2.3 1.8 3.1
Intensity Change (Log2) -0.03 0.5 0.6 0.77 0.93 0.9 1.08 1.11 1.24 0.17 0.59 0.81 1.41 1.32 1.22 -0.31 -0.13 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 2.3 2.9 3.2 3.5 4.0 5.6 2.7 3.6 2.6 3.9 4.7 4.9
Bud 0 0 0 1.6 1.5 2.5 1.9 2.1 2.1 1.6 1.5 0 0 0 3.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.4 2.3 3.4 4.0 3.9 2.0 3.6 4.7 5.8 0 2.2 0 2.0 0 0
Cytoplasm -1.2 -3.8 -5.8 -9.6 -10.2 -11.3 -10.1 -9.0 -12.2 -5.3 -11.2 -16.0 -12.2 -10.7 -9.3
Endoplasmic Reticulum 0 0 2.1 2.5 3.2 2.2 2.7 4.3 4.0 0 0 0 2.6 0 0
Endosome -0.3 0.1 0 0 0 0 0 0 0 6.0 8.3 10.3 3.5 3.6 4.2
Golgi 0 0 0 2.2 0 0 2.4 0 3.3 0 3.4 3.1 7.2 6.6 4.7
Mitochondria 2.8 3.1 5.0 5.5 4.2 7.6 5.7 7.2 4.0 -0.2 0 0 1.5 2.6 3.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.9 0 0 0 0 3.0 4.6 4.8 5.5 3.9
Peroxisomes 0 0 0 0 0 0 0 0 0 3.6 4.8 8.1 0 0 0
SpindlePole 0 -0.2 0 0 0 0 0 0 0 4.3 7.8 11.2 7.0 4.7 3.7
Vacuole 1.4 3.4 3.9 7.3 8.0 7.4 7.8 4.2 8.7 -1.0 1.3 -0.5 5.7 3.9 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.8603 9.0435 8.5599 7.7549 8.4784 8.1531 3.7643 5.2194 4.9187 4.1509 4.0208 4.4879 4.3917 5.6084 5.1914 4.6284 4.4775 5.0062
Actin 0.022 0.0032 0.0045 0.0376 0.0164 0.0017 0.0126 0.009 0.0145 0.001 0.0001 0.0187 0.0418 0.0005 0.007 0.01 0.0492 0.0181
Bud 0.0035 0.0059 0.0073 0.004 0.0095 0.001 0.0008 0.0028 0.0038 0.0007 0.0002 0.0008 0.0018 0.0007 0.0053 0.0003 0.0168 0.0042
Bud Neck 0.0036 0.0004 0.001 0.0012 0.002 0.0016 0.0016 0.0005 0.0048 0.0035 0.0006 0.002 0.0075 0.0002 0.0014 0.0016 0.0086 0.002
Bud Periphery 0.0029 0.0052 0.0043 0.004 0.0067 0.0006 0.0006 0.0014 0.0058 0.0001 0 0.0007 0.0017 0.0003 0.0021 0.0002 0.012 0.0051
Bud Site 0.0059 0.0106 0.0072 0.0032 0.0044 0.0003 0.0048 0.0047 0.0025 0.0018 0.0003 0.0003 0.0317 0.0035 0.0109 0.0019 0.0042 0.0008
Cell Periphery 0.0004 0.0003 0.0003 0.0002 0.0002 0.0001 0.0002 0.0002 0.0003 0.0001 0 0.0001 0.0007 0.0002 0.0003 0.0002 0.0031 0.0002
Cytoplasm 0.8 0.9103 0.8605 0.7278 0.7395 0.8655 0.7189 0.9206 0.8252 0.7125 0.8392 0.8532 0.6062 0.9305 0.9056 0.9065 0.7686 0.8632
Cytoplasmic Foci 0.0324 0.0194 0.0318 0.0556 0.0404 0.0438 0.0569 0.0202 0.0203 0.0475 0.0996 0.035 0.0647 0.0121 0.0084 0.0143 0.0244 0.0188
Eisosomes 0.0002 0 0 0.0001 0.0001 0 0.0002 0 0.0001 0 0 0.0001 0.0002 0 0.0002 0.0001 0.0001 0
Endoplasmic Reticulum 0.0111 0.0105 0.0063 0.0036 0.0058 0.007 0.0097 0.004 0.0067 0.0044 0.0042 0.0156 0.0117 0.0046 0.0033 0.0054 0.0094 0.0029
Endosome 0.043 0.0141 0.0276 0.0957 0.1028 0.0418 0.0475 0.0073 0.0261 0.0706 0.0364 0.0351 0.0735 0.0054 0.0169 0.019 0.0324 0.0447
Golgi 0.0092 0.0016 0.0053 0.0148 0.0275 0.0038 0.014 0.0015 0.0152 0.007 0.0018 0.0053 0.0213 0.0005 0.0029 0.0231 0.0236 0.0066
Lipid Particles 0.0035 0.0001 0.0015 0.0083 0.0074 0.0017 0.0181 0.0002 0.0008 0.0001 0.0001 0.0041 0.009 0.0001 0.0033 0.0031 0.0049 0.0009
Mitochondria 0.0045 0.0007 0.0041 0.0033 0.0131 0.002 0.0063 0.0008 0.0046 0.0011 0.0005 0.002 0.0086 0.0002 0.0078 0.0028 0.0033 0.0107
None 0.0258 0.0075 0.0102 0.0034 0.0024 0.0128 0.0651 0.0203 0.0515 0.1393 0.0105 0.0128 0.0224 0.0355 0.0119 0.0048 0.0138 0.0041
Nuclear Periphery 0.008 0.0018 0.0025 0.0023 0.0025 0.0027 0.0052 0.001 0.0066 0.0015 0.0009 0.0019 0.0336 0.0011 0.0016 0.0011 0.0068 0.002
Nucleolus 0.0003 0.0001 0.004 0.0002 0.0008 0.0002 0.0009 0.0001 0.0001 0 0 0.0001 0.002 0.0001 0.0007 0.0001 0.0006 0.0002
Nucleus 0.0092 0.0034 0.0042 0.0039 0.004 0.0045 0.003 0.0022 0.0039 0.0017 0.0016 0.0024 0.0086 0.0016 0.0025 0.0018 0.0037 0.0017
Peroxisomes 0.0026 0.0004 0.0073 0.0081 0.006 0.0017 0.0183 0.0008 0.0016 0.0016 0.0011 0.0015 0.0108 0.0003 0.0031 0.001 0.0035 0.0012
Punctate Nuclear 0.0018 0.0007 0.0025 0.0104 0.0019 0.0019 0.0077 0.0006 0.0005 0.0005 0.0006 0.0037 0.0306 0.0004 0.0007 0.0005 0.0013 0.0012
Vacuole 0.0079 0.0032 0.0066 0.0101 0.005 0.0041 0.0056 0.0016 0.0037 0.0039 0.0017 0.003 0.0068 0.002 0.003 0.0018 0.008 0.0072
Vacuole Periphery 0.0023 0.0005 0.0013 0.002 0.0017 0.001 0.0016 0.0003 0.0014 0.0012 0.0006 0.0016 0.0048 0.0002 0.0011 0.0006 0.0019 0.0042

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.2056 16.6145 10.382 28.4299 19.6582 13.6872 25.4194 49.2313 50.9295 21.5304
Translational Efficiency 0.8368 1.3693 1.1913 1.1667 1.0837 1.9276 1.2707 1.045 1.0879 0.9977

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
Localization
Cell Percentages cytoplasm (71%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hsp42

Hsp42


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsp42-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available