Standard name | |
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Human Ortholog | |
Description | Translation termination factor eRF3; has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that modifies cellular fitness, alters translational fidelity by affecting reading frame selection, and results in a nonsense suppressor phenotype; many stress-response genes are repressed in the presence of [PSI(+)] |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 6 | 7 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 2 | 1 | 2 | 2 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 4 | 2 | 0 | 9 | 7 | 5 | 4 | 14 | 8 | 5 | 3 | 5 | 5 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 62 | 206 | 131 | 54 | 76 | 87 | 141 | 117 | 47 | 108 | 69 | 72 | 135 | 143 | 144 | – | – | – | 83 | 197 | 123 | 110 | 148 | 147 |
Endoplasmic Reticulum | 0 | 2 | 3 | 1 | 4 | 9 | 13 | 12 | 4 | 12 | 4 | 2 | 4 | 5 | 1 | – | – | – | 0 | 2 | 3 | 2 | 1 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 2 | 1 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 6 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | – | – | – | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 6 | 0 | 1 | 1 | 1 | – | – | – | 0 | 2 | 2 | 2 | 1 | 1 |
Unique Cell Count | 62 | 208 | 135 | 56 | 79 | 100 | 155 | 136 | 54 | 129 | 95 | 82 | 140 | 149 | 146 | 85 | 211 | 134 | 121 | 158 | 168 | |||
Labelled Cell Count | 62 | 212 | 139 | 58 | 80 | 107 | 162 | 137 | 58 | 145 | 106 | 85 | 144 | 155 | 151 | 85 | 211 | 134 | 121 | 158 | 168 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 52.3 | 77.4 | 56.7 | 50.1 | 46.2 | 41.7 | 40.4 | 37.8 | 36.0 | 36.0 | 31.0 | 31.7 | 54.2 | 53.1 | 52.5 | – | – | – | 47.2 | 51.6 | 50.7 |
Std Deviation (1e-4) | 11.0 | 15.8 | 11.0 | 9.7 | 11.3 | 11.0 | 9.3 | 10.4 | 10.2 | 9.0 | 9.8 | 8.7 | 17.5 | 18.8 | 20.8 | – | – | – | 15.9 | 15.6 | 20.8 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.3 | -0.44 | -0.49 | -0.58 | -0.66 | -0.65 | -0.87 | -0.84 | -0.06 | -0.1 | -0.11 | – | – | – | -0.26 | -0.14 | -0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 2.0 | 0.7 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | -0.2 | -0.3 | -2.9 | -2.1 | -3.3 | -2.7 | -3.7 | -5.4 | -2.7 | -0.3 | -0.5 | 0.9 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 2.3 | 2.3 | 2.4 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 32.7692 | 56.266 | 49.9468 | 44.9428 | 45.1867 | 52.0806 | 40.6717 | 55.253 | 56.2708 | 46.0926 | 30.9552 | 53.7072 | 44.386 | 66.7109 | 62.1454 | 53.8799 | 41.6566 | 62.2976 |
Actin | 0.0099 | 0.0018 | 0.0195 | 0.044 | 0.0021 | 0.0011 | 0.0166 | 0.0023 | 0.0146 | 0.0131 | 0.0015 | 0.0022 | 0.0106 | 0.0004 | 0.0028 | 0.0416 | 0.078 | 0.0023 |
Bud | 0.0017 | 0.0005 | 0.0029 | 0.0037 | 0.0066 | 0.0025 | 0.0035 | 0.0008 | 0.0022 | 0.0076 | 0.0024 | 0.002 | 0.005 | 0.0008 | 0.0018 | 0.0027 | 0.0052 | 0.0015 |
Bud Neck | 0.0012 | 0.0016 | 0.0014 | 0.0009 | 0.0011 | 0.0161 | 0.0023 | 0.0007 | 0.0017 | 0.0032 | 0.0011 | 0.005 | 0.0035 | 0.001 | 0.0043 | 0.0034 | 0.0025 | 0.0046 |
Bud Periphery | 0.0018 | 0.0003 | 0.0043 | 0.0074 | 0.0235 | 0.0024 | 0.0068 | 0.0006 | 0.0016 | 0.0263 | 0.0008 | 0.0013 | 0.0116 | 0.0006 | 0.0025 | 0.0045 | 0.0042 | 0.0015 |
Bud Site | 0.0017 | 0.0022 | 0.0059 | 0.004 | 0.0019 | 0.0004 | 0.0028 | 0.0021 | 0.0046 | 0.0012 | 0.0058 | 0.0004 | 0.0056 | 0.001 | 0.0022 | 0.0049 | 0.003 | 0.0003 |
Cell Periphery | 0.0061 | 0.0248 | 0.0145 | 0.0707 | 0.0014 | 0.0043 | 0.0134 | 0.0158 | 0.016 | 0.0122 | 0.0036 | 0.0035 | 0.0516 | 0.0144 | 0.0187 | 0.0223 | 0.0041 | 0.0075 |
Cytoplasm | 0.6326 | 0.8885 | 0.7853 | 0.3927 | 0.8378 | 0.8565 | 0.6738 | 0.874 | 0.8288 | 0.5093 | 0.6094 | 0.8764 | 0.5785 | 0.8925 | 0.799 | 0.5893 | 0.6539 | 0.8799 |
Cytoplasmic Foci | 0.0107 | 0.0006 | 0.0024 | 0.006 | 0.0014 | 0.0034 | 0.0031 | 0.0009 | 0.0025 | 0.0034 | 0.1178 | 0.0036 | 0.0109 | 0.0009 | 0.0012 | 0.0063 | 0.0077 | 0.0031 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0009 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0004 | 0 | 0.0004 | 0 | 0.0001 | 0.001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0518 | 0.0081 | 0.0062 | 0.0066 | 0.0022 | 0.0251 | 0.0563 | 0.003 | 0.0108 | 0.0065 | 0.0044 | 0.0304 | 0.05 | 0.0081 | 0.0209 | 0.0044 | 0.0106 | 0.0351 |
Endosome | 0.0378 | 0.0008 | 0.0104 | 0.003 | 0.0081 | 0.0074 | 0.0217 | 0.0017 | 0.005 | 0.0664 | 0.023 | 0.017 | 0.0435 | 0.0015 | 0.0034 | 0.0178 | 0.0441 | 0.0055 |
Golgi | 0.0051 | 0.0002 | 0.0024 | 0.0015 | 0.0014 | 0.0008 | 0.0024 | 0.0002 | 0.0009 | 0.0031 | 0.0018 | 0.0033 | 0.0146 | 0.0001 | 0.0003 | 0.0038 | 0.0266 | 0.0017 |
Lipid Particles | 0.0065 | 0.0011 | 0.0006 | 0.0051 | 0.0004 | 0.0003 | 0.0014 | 0.0017 | 0.0013 | 0.0013 | 0.0162 | 0.0008 | 0.0078 | 0.0014 | 0.0009 | 0.0049 | 0.0018 | 0.0012 |
Mitochondria | 0.0124 | 0.0001 | 0.0062 | 0.0561 | 0.061 | 0.0005 | 0.0087 | 0.0154 | 0.0005 | 0.0145 | 0.0065 | 0.001 | 0.0067 | 0.0001 | 0.0002 | 0.0087 | 0.008 | 0.0012 |
None | 0.003 | 0.0001 | 0.0007 | 0.0218 | 0.0003 | 0.0002 | 0.0024 | 0.0007 | 0.0002 | 0.0004 | 0.0465 | 0.0005 | 0.0026 | 0.0003 | 0.0002 | 0.0084 | 0.0012 | 0.0004 |
Nuclear Periphery | 0.0754 | 0.0033 | 0.0109 | 0.0092 | 0.0031 | 0.0049 | 0.0294 | 0.0021 | 0.0051 | 0.0064 | 0.0072 | 0.007 | 0.0365 | 0.0028 | 0.0049 | 0.0102 | 0.0196 | 0.0061 |
Nucleolus | 0.0016 | 0.0002 | 0.0004 | 0.0022 | 0.0005 | 0.0001 | 0.0007 | 0.0006 | 0.0002 | 0.0005 | 0.0142 | 0.0003 | 0.0013 | 0.0002 | 0.0002 | 0.0115 | 0.0003 | 0.0004 |
Nucleus | 0.0247 | 0.0021 | 0.0129 | 0.0053 | 0.0038 | 0.0034 | 0.0132 | 0.0023 | 0.0032 | 0.0046 | 0.011 | 0.004 | 0.0121 | 0.0021 | 0.0026 | 0.01 | 0.0485 | 0.0063 |
Peroxisomes | 0.0005 | 0 | 0.0002 | 0.0013 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0017 | 0.0001 | 0.0009 | 0 | 0 | 0.0011 | 0.0032 | 0.0001 |
Punctate Nuclear | 0.0011 | 0 | 0.0008 | 0.0008 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0125 | 0.0002 | 0.001 | 0 | 0.0001 | 0.0014 | 0.0008 | 0.0002 |
Vacuole | 0.0946 | 0.0618 | 0.0958 | 0.3278 | 0.0326 | 0.0628 | 0.126 | 0.0673 | 0.0944 | 0.2876 | 0.0895 | 0.0327 | 0.1346 | 0.0687 | 0.1231 | 0.2059 | 0.0652 | 0.0358 |
Vacuole Periphery | 0.0198 | 0.0019 | 0.0162 | 0.0291 | 0.0102 | 0.0076 | 0.0144 | 0.0069 | 0.0061 | 0.0323 | 0.0228 | 0.0083 | 0.0106 | 0.0031 | 0.0108 | 0.036 | 0.0113 | 0.0053 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 190.6027 | 172.5439 | 144.3951 | 163.629 | 181.4333 | 142.0021 | 141.8691 | 148.984 | 140.3417 | 158.7992 |
Translational Efficiency | 2.2733 | 2.4678 | 2.4435 | 1.9632 | 1.9055 | 2.3061 | 2.129 | 1.8635 | 1.8122 | 1.8628 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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