Standard name | |
---|---|
Human Ortholog | |
Description | Inositol polyphosphate multikinase (IPMK); sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 7 | 2 | 8 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 2 | 1 | 3 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 224 | 201 | 107 | 168 | 196 | 284 | 251 | 214 | 212 | 254 | 221 | 188 | 195 | 234 | 212 | 157 | 164 | 205 | 217 | 191 | 128 | 167 | 296 | 262 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 6 | 4 | 6 | 0 | 0 | 0 | 1 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 3 | 2 | 2 | 1 | 1 | 0 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 |
Mitochondria | 19 | 3 | 4 | 11 | 4 | 42 | 103 | 57 | 123 | 105 | 159 | 92 | 0 | 0 | 0 | 3 | 3 | 4 | 4 | 3 | 6 | 4 | 3 | 4 |
Nucleus | 0 | 2 | 1 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 4 | 5 | 3 | 1 | 3 | 9 | 1 | 6 | 0 | 4 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 3 | 1 |
Vac/Vac Membrane | 9 | 3 | 1 | 2 | 0 | 2 | 1 | 2 | 6 | 11 | 7 | 9 | 3 | 6 | 9 | 81 | 87 | 87 | 6 | 5 | 2 | 3 | 8 | 3 |
Unique Cell Count | 238 | 206 | 112 | 171 | 198 | 297 | 289 | 253 | 277 | 320 | 318 | 259 | 198 | 236 | 217 | 205 | 221 | 279 | 243 | 213 | 146 | 194 | 331 | 293 |
Labelled Cell Count | 254 | 211 | 114 | 183 | 202 | 333 | 360 | 281 | 350 | 379 | 406 | 310 | 203 | 242 | 225 | 262 | 264 | 311 | 243 | 213 | 146 | 194 | 331 | 293 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.9 | 4.7 | 4.5 | 4.7 | 3.7 | 3.4 | 4.2 | 3.3 | 3.5 | 3.2 | 3.6 | 4.8 | 5.4 | 5.5 | 7.1 | 6.9 | 6.6 | 4.9 | 5.2 | 5.7 |
Std Deviation (1e-4) | 0.7 | 0.6 | 1.2 | 1.3 | 1.6 | 1.3 | 1.0 | 1.5 | 1.1 | 1.0 | 1.1 | 0.9 | 0.6 | 1.2 | 1.7 | 1.0 | 1.4 | 1.4 | 1.6 | 1.4 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.02 | -0.32 | -0.44 | -0.16 | -0.49 | -0.4 | -0.55 | -0.38 | 0.06 | 0.21 | 0.24 | 0.62 | 0.58 | 0.51 | 0.06 | 0.17 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.3 | 2.0 | 0 | -2.5 | -3.0 | -4.4 | -4.0 | -5.6 | -5.0 | 1.6 | 2.2 | 1.1 | -4.3 | -4.7 | -4.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.5 | 7.5 | 6.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8633 | 2.072 | 1.6969 | 1.7569 | 1.7683 | 1.7043 | -0.0081 | 0.2279 | 0.0667 | 0.1074 | -0.2461 | 0.1139 | -0.3774 | -0.2818 | -0.5371 | -0.189 | -1.4105 | -0.4019 |
Actin | 0.0096 | 0.0005 | 0.0033 | 0.0009 | 0.0072 | 0.0017 | 0.0215 | 0.0007 | 0.0062 | 0.0174 | 0.0007 | 0.0029 | 0.0449 | 0.009 | 0.0084 | 0.0002 | 0.0001 | 0.0019 |
Bud | 0.0007 | 0.0003 | 0.0009 | 0.0001 | 0.001 | 0.0005 | 0.0025 | 0.0017 | 0.0086 | 0.001 | 0.0003 | 0.0006 | 0.0013 | 0.0012 | 0.0015 | 0.0005 | 0 | 0.0012 |
Bud Neck | 0.0034 | 0.0003 | 0.0015 | 0.0007 | 0.001 | 0.0028 | 0.0069 | 0.0004 | 0.0022 | 0.0024 | 0.0007 | 0.0015 | 0.0027 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0008 |
Bud Periphery | 0.0007 | 0.0003 | 0.0012 | 0.0001 | 0.001 | 0.0006 | 0.0028 | 0.0008 | 0.0041 | 0.0013 | 0.0003 | 0.0007 | 0.0017 | 0.0012 | 0.0071 | 0.0008 | 0 | 0.0017 |
Bud Site | 0.0075 | 0.0009 | 0.0041 | 0.0006 | 0.0009 | 0.001 | 0.0047 | 0.012 | 0.009 | 0.0119 | 0.0002 | 0.0002 | 0.0067 | 0.0056 | 0.0006 | 0.0025 | 0 | 0.0002 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0005 | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0004 | 0 | 0 |
Cytoplasm | 0.3224 | 0.3311 | 0.3262 | 0.3873 | 0.3582 | 0.4362 | 0.1898 | 0.2528 | 0.2155 | 0.1986 | 0.266 | 0.2856 | 0.2224 | 0.336 | 0.4036 | 0.3645 | 0.5501 | 0.5369 |
Cytoplasmic Foci | 0.0516 | 0.005 | 0.0221 | 0.0285 | 0.0406 | 0.0122 | 0.0221 | 0.0056 | 0.0112 | 0.0193 | 0.0124 | 0.0116 | 0.0475 | 0.0105 | 0.0059 | 0.0072 | 0.0041 | 0.0317 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0004 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.007 | 0.0045 | 0.0032 | 0.0021 | 0.0036 | 0.0039 | 0.0037 | 0.0038 | 0.0029 | 0.0033 | 0.001 | 0.0017 | 0.0073 | 0.0021 | 0.0088 | 0.004 | 0.0023 | 0.0037 |
Endosome | 0.0466 | 0.0086 | 0.0197 | 0.0412 | 0.068 | 0.0067 | 0.0126 | 0.0023 | 0.0075 | 0.0186 | 0.0014 | 0.0061 | 0.0355 | 0.002 | 0.0062 | 0.0372 | 0.0014 | 0.014 |
Golgi | 0.0109 | 0.001 | 0.0078 | 0.0117 | 0.0417 | 0.0037 | 0.0027 | 0.0001 | 0.002 | 0.0098 | 0.0002 | 0.0009 | 0.0159 | 0.0004 | 0.0015 | 0.0021 | 0 | 0.0117 |
Lipid Particles | 0.0102 | 0.0003 | 0.0129 | 0.0061 | 0.0063 | 0.0026 | 0.0085 | 0.0003 | 0.0036 | 0.0124 | 0.0004 | 0.001 | 0.0114 | 0.0004 | 0.0007 | 0.0016 | 0 | 0.0025 |
Mitochondria | 0.0104 | 0.0096 | 0.0078 | 0.0015 | 0.0557 | 0.0027 | 0.0067 | 0.0006 | 0.0093 | 0.0544 | 0.0004 | 0.0034 | 0.0178 | 0.0015 | 0.0155 | 0.0035 | 0.0001 | 0.0009 |
None | 0.3931 | 0.4154 | 0.4586 | 0.2602 | 0.1468 | 0.3091 | 0.6597 | 0.6815 | 0.6456 | 0.53 | 0.6778 | 0.6194 | 0.5425 | 0.6147 | 0.5242 | 0.5188 | 0.4312 | 0.3702 |
Nuclear Periphery | 0.02 | 0.0092 | 0.0057 | 0.0039 | 0.0073 | 0.0075 | 0.0124 | 0.0042 | 0.0098 | 0.0118 | 0.0016 | 0.0081 | 0.006 | 0.0018 | 0.0033 | 0.0045 | 0.0013 | 0.0024 |
Nucleolus | 0.0008 | 0.0005 | 0.0015 | 0.0004 | 0.0007 | 0.0017 | 0.0019 | 0.001 | 0.0013 | 0.007 | 0.0005 | 0.0011 | 0.0006 | 0.0002 | 0.0002 | 0.0008 | 0 | 0.0003 |
Nucleus | 0.0704 | 0.205 | 0.0957 | 0.2367 | 0.2204 | 0.1729 | 0.0171 | 0.0212 | 0.0318 | 0.0316 | 0.0051 | 0.0244 | 0.0171 | 0.0055 | 0.0052 | 0.0042 | 0.0071 | 0.0096 |
Peroxisomes | 0.0172 | 0.0005 | 0.0154 | 0.01 | 0.0209 | 0.0053 | 0.0069 | 0.0003 | 0.0034 | 0.0269 | 0.0016 | 0.0007 | 0.0107 | 0.0024 | 0.0004 | 0.0002 | 0 | 0.0026 |
Punctate Nuclear | 0.0104 | 0.0037 | 0.0085 | 0.0056 | 0.011 | 0.027 | 0.0137 | 0.0086 | 0.0232 | 0.0363 | 0.0288 | 0.0284 | 0.003 | 0.0039 | 0.0011 | 0.0012 | 0.0014 | 0.0061 |
Vacuole | 0.0044 | 0.0023 | 0.0031 | 0.002 | 0.0042 | 0.0014 | 0.0021 | 0.0014 | 0.0016 | 0.0041 | 0.0004 | 0.0009 | 0.0024 | 0.0013 | 0.003 | 0.0379 | 0.0006 | 0.0013 |
Vacuole Periphery | 0.0023 | 0.0008 | 0.0007 | 0.0004 | 0.0036 | 0.0003 | 0.0009 | 0.0003 | 0.0007 | 0.0013 | 0.0001 | 0.0008 | 0.0013 | 0.0002 | 0.0017 | 0.0077 | 0.0001 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.431 | 9.2357 | 12.336 | 11.4168 | 9.3767 | 22.7167 | 10.5055 | 11.3448 | 15.6273 | 13.3474 |
Translational Efficiency | 1.2571 | 2.4619 | 1.2185 | 2.3632 | 3.3281 | 0.9374 | 2.0534 | 2.1107 | 1.3168 | 1.1876 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|