Standard name
Human Ortholog
Description Chromatin associated high mobility group (HMG) family member; involved in compacting, bending, bridging and looping DNA; rDNA-binding component that regulates transcription from RNA polymerase I promoters; regulates start site selection of ribosomal protein genes via RNA polymerase II promoters; role in genome maintenance; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase; relocalizes to the cytosol in response to hypoxia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0.07 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.37 0.33 0.27 0.25 0.21 0.24 0.22 0.15 0.33 0.2 0.28 0.29 0.25 0.21 0.2 0.07 0.06 0 0 0 0 0 0 0
Nucleus 0.13 0.09 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0.06 0.13 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.92 0.94 0.95 0.94 0.94 0.92 0.93 0.93 0.91 0.94 0.95 0.94 0.94 0.9 0.84 0.88 0.92 0.95 0.85 0.79 0.88 0.85 0.87 0.79
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0.07 0.09 0.06 0 0.06 0.05 0.08 0 0 0 0 0 0 0.05 0 0 0.06 0 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 6
Bud Neck 0 0 1 3 2 0 6 4 2 2 6 2 2 7 5 0 0 0 0 0 2 0 6 5
Bud Site 3 0 0 0 1 1 4 0 3 1 0 2 0 1 0 0 0 0
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 10 6 10 4 4 8 6 8 2 7 35 66 10 8 3 0 0 0 0 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 2 0 0 0 0 0 1
Endosome 0 0 0 0 1 4 1 2 1 0 0 2 0 1 7 1 1 1 2 0 0 2 3 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 86 18 47 60 57 75 98 82 143 69 113 139 70 115 120 10 10 3 1 0 0 2 4 7
Nucleus 30 5 11 7 7 6 10 17 10 8 8 13 13 14 13 8 6 5 13 9 6 3 14 5
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 1 2
Nucleolus 212 51 162 221 259 291 409 514 395 318 386 457 259 497 502 133 144 110 197 56 162 154 416 320
Peroxisomes 1 0 0 0 3 14 6 11 2 5 1 9 3 6 12 2 2 0 0 0 0 0 0 1
SpindlePole 2 0 3 9 14 22 38 32 19 22 21 40 4 21 9 1 2 0 11 2 5 10 19 32
Vac/Vac Membrane 0 0 0 1 0 2 2 5 3 1 2 0 0 3 3 1 0 1 2 0 2 2 5 9
Unique Cell Count 231 54 171 236 276 316 441 555 435 339 407 486 277 550 598 151 156 116 233 71 184 182 481 408
Labelled Cell Count 336 74 224 311 350 425 578 672 586 432 547 667 358 700 738 169 175 125 233 71 184 182 481 408


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 32.7 22.3 31.0 31.4 30.2 25.5 23.9 23.7 24.3 21.4 22.2 22.4 32.0 31.5 29.9 28.2 36.8 44.9 30.7 35.8 32.5
Std Deviation (1e-4) 14.5 10.4 11.9 14.2 12.1 11.3 9.9 8.9 11.7 8.3 9.7 9.6 14.7 14.7 16.0 13.7 18.2 21.7 12.9 15.4 14.4
Intensity Change (Log2) 0.02 -0.04 -0.28 -0.38 -0.39 -0.35 -0.54 -0.48 -0.47 0.04 0.02 -0.06 -0.14 0.25 0.53 -0.02 0.21 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 3.4 4.5 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.5 -1.7 -0.9 -1.4 -3.8 1.3 -1.8 0.1 0.3 -0.5 -1.8 -2.1 -4.9 -5.0 -5.5
Nucleus -1.7 -2.0 -2.6 -2.5 -2.0 -2.5 -2.3 -2.7 -2.3 -0.8 -2.4 -2.8 -0.4 -1.1 -0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.5 -0.4 -1.1 -0.9 -1.0 -1.6 -0.4 0.1 -0.3 -0.5 -1.8 -3.6 -2.2 -0.9 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.2 1.8 2.5 3.0 2.1 1.5 2.3 1.9 2.9 0 1.3 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 49.5975 36.7066 37.5399 36.1626 29.3849 35.5295 54.4762 37.2504 40.2383 44.0627 38.127 38.508 60.0207 42.3803 44.4236 41.8563 42.2533 41.0286
Actin 0.0196 0 0.0043 0.0021 0.0027 0 0.0029 0 0.0023 0 0.0042 0.004 0.0079 0 0.0068 0 0.0018 0
Bud 0.0003 0 0.0003 0 0.0002 0 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0
Bud Neck 0.0009 0 0.0002 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0013 0 0.0001 0 0.0001 0
Bud Periphery 0.0005 0 0.0003 0 0.0006 0 0 0 0 0 0.0001 0.0001 0.0001 0 0 0 0.0001 0
Bud Site 0.001 0 0.0038 0.0001 0.001 0 0.0001 0.0002 0.0002 0 0.0002 0.0001 0.0018 0.0001 0 0 0.0002 0
Cell Periphery 0.0003 0.0001 0.0003 0 0.0002 0.0001 0.0002 0.0001 0.0002 0.0002 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001
Cytoplasm 0.0005 0 0.0014 0 0.0001 0.0001 0.0015 0 0.0015 0 0.0001 0.0001 0.0008 0 0 0 0.0001 0
Cytoplasmic Foci 0.0163 0.0011 0.0115 0.0036 0.0098 0.0146 0.0139 0.01 0.0029 0.0032 0.0206 0.0014 0.0091 0.0003 0.0008 0.0003 0.0023 0.0012
Eisosomes 0.0002 0 0.0006 0 0.0002 0 0.0002 0 0.0001 0 0.0003 0 0.0002 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0007 0 0.0008 0 0.0001 0 0.0003 0 0.0003 0 0 0 0.0003 0 0 0 0.0009 0
Endosome 0.0246 0 0.0039 0.0007 0.0055 0 0.0016 0 0.0021 0 0.0014 0.0003 0.0017 0 0.0001 0 0.01 0
Golgi 0.0139 0 0.002 0.0015 0.0053 0 0.0011 0 0.0013 0 0.0024 0.0004 0.0006 0 0.0002 0 0.0024 0
Lipid Particles 0.0125 0.0005 0.0108 0.0171 0.0312 0.0061 0.0076 0.0017 0.0043 0.0011 0.022 0.0024 0.0042 0.0003 0.0007 0.0003 0.0111 0.004
Mitochondria 0.011 0.0007 0.0069 0.0011 0.0818 0.0087 0.0061 0.0002 0.0018 0.0018 0.0541 0.0065 0.0076 0.0008 0.0023 0.0004 0.0544 0.0011
None 0.0008 0 0.0047 0.0001 0.0002 0.0005 0.0045 0 0.0131 0 0.0003 0 0.0011 0 0 0 0.0004 0
Nuclear Periphery 0.0036 0 0.002 0 0.0005 0 0.0007 0.0001 0.0006 0 0.0002 0 0.0003 0 0 0 0.008 0
Nucleolus 0.842 0.9415 0.8982 0.9225 0.8088 0.9351 0.9213 0.926 0.9357 0.9623 0.8533 0.9472 0.923 0.9384 0.9494 0.941 0.8687 0.9538
Nucleus 0.0254 0.0457 0.0426 0.0305 0.0255 0.0318 0.0267 0.0527 0.0303 0.0298 0.0229 0.0337 0.0318 0.0596 0.0382 0.0547 0.0317 0.0388
Peroxisomes 0.0072 0 0.0019 0.0198 0.0138 0.0008 0.0089 0.0001 0.001 0.0002 0.0115 0.0002 0.0041 0 0.0002 0 0.002 0.0001
Punctate Nuclear 0.0054 0.0099 0.0018 0.0007 0.0103 0.0017 0.0017 0.0087 0.002 0.0011 0.0042 0.0032 0.0028 0.0001 0.0004 0.003 0.0014 0.0006
Vacuole 0.0089 0.0001 0.0007 0.0001 0.0009 0.0002 0.0002 0.0001 0.0003 0.0002 0.0003 0.0001 0.0004 0.0001 0.0001 0.0001 0.0009 0.0001
Vacuole Periphery 0.0045 0.0001 0.0012 0 0.0011 0.0003 0.0004 0 0.0002 0.0002 0.0014 0.0001 0.0005 0.0001 0.0002 0 0.0029 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 93.8117 89.1865 131.3727 85.9538 111.9777 132.4543 95.2236 105.8289 114.5142 99.8291
Translational Efficiency 0.7007 0.6534 0.6713 1.251 0.746 0.8449 0.768 0.862 1.0298 1.3503

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Chromatin associated high mobility group (HMG) family member; involved in compacting, bending, bridging and looping DNA; rDNA-binding component that regulates transcription from RNA polymerase I promoters; regulates start site selection of ribosomal protein genes via RNA polymerase II promoters; role in genome maintenance; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleolus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Hmo1

Hmo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hmo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available