Standard name | |
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Human Ortholog | |
Description | Chromatin associated high mobility group (HMG) family member; involved in compacting, bending, bridging and looping DNA; rDNA-binding component that regulates transcription from RNA polymerase I promoters; regulates start site selection of ribosomal protein genes via RNA polymerase II promoters; role in genome maintenance; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 6 |
Bud Neck | 0 | 0 | 1 | 3 | 2 | 0 | 6 | 4 | 2 | 2 | 6 | 2 | 2 | 7 | 5 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 6 | 5 |
Bud Site | 3 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 3 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 10 | 6 | 10 | 4 | 4 | 8 | 6 | 8 | 2 | 7 | 35 | 66 | 10 | 8 | 3 | 0 | 0 | 0 | 0 | 0 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 7 | 1 | 1 | 1 | 2 | 0 | 0 | 2 | 3 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 86 | 18 | 47 | 60 | 57 | 75 | 98 | 82 | 143 | 69 | 113 | 139 | 70 | 115 | 120 | 10 | 10 | 3 | 1 | 0 | 0 | 2 | 4 | 7 |
Nucleus | 30 | 5 | 11 | 7 | 7 | 6 | 10 | 17 | 10 | 8 | 8 | 13 | 13 | 14 | 13 | 8 | 6 | 5 | 13 | 9 | 6 | 3 | 14 | 5 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 212 | 51 | 162 | 221 | 259 | 291 | 409 | 514 | 395 | 318 | 386 | 457 | 259 | 497 | 502 | 133 | 144 | 110 | 197 | 56 | 162 | 154 | 416 | 320 |
Peroxisomes | 1 | 0 | 0 | 0 | 3 | 14 | 6 | 11 | 2 | 5 | 1 | 9 | 3 | 6 | 12 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 2 | 0 | 3 | 9 | 14 | 22 | 38 | 32 | 19 | 22 | 21 | 40 | 4 | 21 | 9 | 1 | 2 | 0 | 11 | 2 | 5 | 10 | 19 | 32 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 5 | 3 | 1 | 2 | 0 | 0 | 3 | 3 | 1 | 0 | 1 | 2 | 0 | 2 | 2 | 5 | 9 |
Unique Cell Count | 231 | 54 | 171 | 236 | 276 | 316 | 441 | 555 | 435 | 339 | 407 | 486 | 277 | 550 | 598 | 151 | 156 | 116 | 233 | 71 | 184 | 182 | 481 | 408 |
Labelled Cell Count | 336 | 74 | 224 | 311 | 350 | 425 | 578 | 672 | 586 | 432 | 547 | 667 | 358 | 700 | 738 | 169 | 175 | 125 | 233 | 71 | 184 | 182 | 481 | 408 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 32.7 | 22.3 | 31.0 | 31.4 | 30.2 | 25.5 | 23.9 | 23.7 | 24.3 | 21.4 | 22.2 | 22.4 | 32.0 | 31.5 | 29.9 | 28.2 | 36.8 | 44.9 | 30.7 | 35.8 | 32.5 |
Std Deviation (1e-4) | 14.5 | 10.4 | 11.9 | 14.2 | 12.1 | 11.3 | 9.9 | 8.9 | 11.7 | 8.3 | 9.7 | 9.6 | 14.7 | 14.7 | 16.0 | 13.7 | 18.2 | 21.7 | 12.9 | 15.4 | 14.4 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.04 | -0.28 | -0.38 | -0.39 | -0.35 | -0.54 | -0.48 | -0.47 | 0.04 | 0.02 | -0.06 | -0.14 | 0.25 | 0.53 | -0.02 | 0.21 | 0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 4.5 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.5 | -1.7 | -0.9 | -1.4 | -3.8 | 1.3 | -1.8 | 0.1 | 0.3 | -0.5 | -1.8 | -2.1 | -4.9 | -5.0 | -5.5 |
Nucleus | -1.7 | -2.0 | -2.6 | -2.5 | -2.0 | -2.5 | -2.3 | -2.7 | -2.3 | -0.8 | -2.4 | -2.8 | -0.4 | -1.1 | -0.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.5 | -0.4 | -1.1 | -0.9 | -1.0 | -1.6 | -0.4 | 0.1 | -0.3 | -0.5 | -1.8 | -3.6 | -2.2 | -0.9 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.2 | 1.8 | 2.5 | 3.0 | 2.1 | 1.5 | 2.3 | 1.9 | 2.9 | 0 | 1.3 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 49.5975 | 36.7066 | 37.5399 | 36.1626 | 29.3849 | 35.5295 | 54.4762 | 37.2504 | 40.2383 | 44.0627 | 38.127 | 38.508 | 60.0207 | 42.3803 | 44.4236 | 41.8563 | 42.2533 | 41.0286 |
Actin | 0.0196 | 0 | 0.0043 | 0.0021 | 0.0027 | 0 | 0.0029 | 0 | 0.0023 | 0 | 0.0042 | 0.004 | 0.0079 | 0 | 0.0068 | 0 | 0.0018 | 0 |
Bud | 0.0003 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0009 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0013 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Periphery | 0.0005 | 0 | 0.0003 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Site | 0.001 | 0 | 0.0038 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0018 | 0.0001 | 0 | 0 | 0.0002 | 0 |
Cell Periphery | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Cytoplasm | 0.0005 | 0 | 0.0014 | 0 | 0.0001 | 0.0001 | 0.0015 | 0 | 0.0015 | 0 | 0.0001 | 0.0001 | 0.0008 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0163 | 0.0011 | 0.0115 | 0.0036 | 0.0098 | 0.0146 | 0.0139 | 0.01 | 0.0029 | 0.0032 | 0.0206 | 0.0014 | 0.0091 | 0.0003 | 0.0008 | 0.0003 | 0.0023 | 0.0012 |
Eisosomes | 0.0002 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0007 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0009 | 0 |
Endosome | 0.0246 | 0 | 0.0039 | 0.0007 | 0.0055 | 0 | 0.0016 | 0 | 0.0021 | 0 | 0.0014 | 0.0003 | 0.0017 | 0 | 0.0001 | 0 | 0.01 | 0 |
Golgi | 0.0139 | 0 | 0.002 | 0.0015 | 0.0053 | 0 | 0.0011 | 0 | 0.0013 | 0 | 0.0024 | 0.0004 | 0.0006 | 0 | 0.0002 | 0 | 0.0024 | 0 |
Lipid Particles | 0.0125 | 0.0005 | 0.0108 | 0.0171 | 0.0312 | 0.0061 | 0.0076 | 0.0017 | 0.0043 | 0.0011 | 0.022 | 0.0024 | 0.0042 | 0.0003 | 0.0007 | 0.0003 | 0.0111 | 0.004 |
Mitochondria | 0.011 | 0.0007 | 0.0069 | 0.0011 | 0.0818 | 0.0087 | 0.0061 | 0.0002 | 0.0018 | 0.0018 | 0.0541 | 0.0065 | 0.0076 | 0.0008 | 0.0023 | 0.0004 | 0.0544 | 0.0011 |
None | 0.0008 | 0 | 0.0047 | 0.0001 | 0.0002 | 0.0005 | 0.0045 | 0 | 0.0131 | 0 | 0.0003 | 0 | 0.0011 | 0 | 0 | 0 | 0.0004 | 0 |
Nuclear Periphery | 0.0036 | 0 | 0.002 | 0 | 0.0005 | 0 | 0.0007 | 0.0001 | 0.0006 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0 | 0.008 | 0 |
Nucleolus | 0.842 | 0.9415 | 0.8982 | 0.9225 | 0.8088 | 0.9351 | 0.9213 | 0.926 | 0.9357 | 0.9623 | 0.8533 | 0.9472 | 0.923 | 0.9384 | 0.9494 | 0.941 | 0.8687 | 0.9538 |
Nucleus | 0.0254 | 0.0457 | 0.0426 | 0.0305 | 0.0255 | 0.0318 | 0.0267 | 0.0527 | 0.0303 | 0.0298 | 0.0229 | 0.0337 | 0.0318 | 0.0596 | 0.0382 | 0.0547 | 0.0317 | 0.0388 |
Peroxisomes | 0.0072 | 0 | 0.0019 | 0.0198 | 0.0138 | 0.0008 | 0.0089 | 0.0001 | 0.001 | 0.0002 | 0.0115 | 0.0002 | 0.0041 | 0 | 0.0002 | 0 | 0.002 | 0.0001 |
Punctate Nuclear | 0.0054 | 0.0099 | 0.0018 | 0.0007 | 0.0103 | 0.0017 | 0.0017 | 0.0087 | 0.002 | 0.0011 | 0.0042 | 0.0032 | 0.0028 | 0.0001 | 0.0004 | 0.003 | 0.0014 | 0.0006 |
Vacuole | 0.0089 | 0.0001 | 0.0007 | 0.0001 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0001 |
Vacuole Periphery | 0.0045 | 0.0001 | 0.0012 | 0 | 0.0011 | 0.0003 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0014 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0029 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 93.8117 | 89.1865 | 131.3727 | 85.9538 | 111.9777 | 132.4543 | 95.2236 | 105.8289 | 114.5142 | 99.8291 |
Translational Efficiency | 0.7007 | 0.6534 | 0.6713 | 1.251 | 0.746 | 0.8449 | 0.768 | 0.862 | 1.0298 | 1.3503 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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