Standard name
Human Ortholog
Description Subunit of chromatin modifying histone acetyltransferase complexes; member of the ADA complex, the SAGA complex, and the SLIK complex; transcriptional regulator involved in glucose repression of Gal4p-regulated genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.09 0.07 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.25 0 0.07 0 0.22 0.17 0.1 0.29 0.34 0.4 0.18 0 0 0 0.09 0 0.07 0 0 0 0 0 0
Nucleus 0.87 0.87 0.97 0.96 0.94 0.89 0.89 0.83 0.78 0.75 0.69 0.77 0.89 0.88 0.84 0.81 0.83 0.81 0.8 0.85 0.85 0.74 0.72 0.73
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.24 0.15 0 0 0 0.09 0.09 0.12 0.19 0.19 0.19 0.22 0.21 0.13 0.18 0.1 0.11 0.12 0.13 0.05 0.09 0.17 0.19 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 1 0 0 0 1 6 4 5 13 8 8 4 0 0 0 0 0 0 1 1 0 1 0 2
Bud Neck 0 0 0 0 2 0 2 4 1 4 3 5 0 0 0 0 0 0 0 1 1 0 2 1
Bud Site 0 0 0 1 8 6 10 4 16 14 8 6 1 3 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 4 4 1 7 17 10 17 21 16 19 19 8 22 30 45 11 11 8 0 0 0 0 0 0
Endoplasmic Reticulum 0 1 1 0 0 1 1 1 0 1 0 0 0 2 0 2 2 2 0 0 0 0 2 2
Endosome 0 0 2 0 2 2 1 4 0 0 0 0 0 0 1 2 2 0 4 1 1 1 2 2
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 2 1 0 1 2
Mitochondria 31 64 2 32 29 141 154 84 207 299 316 110 17 11 10 15 1 11 1 5 1 1 1 3
Nucleus 264 222 499 445 648 566 823 675 551 649 544 472 421 442 427 129 131 123 259 218 468 154 258 272
Nuclear Periphery 1 1 1 0 8 3 7 7 5 12 8 4 6 4 0 3 1 1 0 0 1 0 0 3
Nucleolus 73 38 22 19 27 57 85 95 135 161 148 133 99 66 92 16 17 19 42 13 51 36 67 49
Peroxisomes 0 0 0 1 0 0 0 0 0 1 0 0 0 3 5 0 0 0 0 0 0 0 0 0
SpindlePole 0 2 20 13 9 8 23 21 6 14 12 16 1 9 6 4 3 7 4 1 3 1 1 6
Vac/Vac Membrane 6 0 2 0 3 2 2 15 5 10 16 10 0 1 5 3 2 6 6 9 16 9 18 22
Unique Cell Count 304 254 516 463 688 639 922 811 706 867 785 614 472 500 507 160 157 152 324 257 549 210 357 375
Labelled Cell Count 381 332 551 518 755 803 1129 936 956 1192 1082 768 567 572 591 186 170 178 324 257 549 210 357 375


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 5.4 6.5 5.7 6.4 5.0 5.2 5.7 4.9 4.8 4.8 5.3 6.5 6.4 6.3 9.0 9.3 9.1 5.8 6.1 6.6
Std Deviation (1e-4) 1.6 1.1 0.9 0.9 1.2 0.9 1.1 1.1 1.2 1.1 1.3 0.9 1.5 1.4 1.6 2.3 2.1 2.3 1.4 1.3 1.4
Intensity Change (Log2) – – – -0.18 -0.02 -0.38 -0.31 -0.2 -0.42 -0.45 -0.44 -0.31 0.01 -0.03 -0.04 0.48 0.51 0.49 -0.15 -0.1 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3.2 0 2.7 3.3 3.1 3.0 3.2 0 4.7 5.4 6.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.6 4.1 11.1 9.5 7.2 0 0 0 9.8 3.7 2.6 2.4 0 0 0
Nucleus -0.5 -2.0 -5.1 -5.0 -7.5 -9.3 -10.5 -12.1 -9.6 -4.7 -5.1 -6.8 -6.9 -6.0 -6.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.1 -0.3 3.1 3.4 4.7 0 0 0 8.5 8.0 5.1 7.1 2.8 3.1 3.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.9 -2.9 -2.9 -1.5 -1.3 0 0 0 -1.2 -4.0 -2.0 -2.7 -0.8 -1.2 0.4
Vacuole 0 0 0 0 2.3 0 0 2.5 2.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.097 2.0936 1.7538 1.6043 1.4376 1.9109 2.0651 2.2375 2.0333 1.9109 1.953 1.816 2.4415 2.2941 1.7629 2.1446 2.0865 1.7509
Actin 0 0 0 0 0 0 0.0054 0 0.0027 0.005 0.0035 0.0021 0.0059 0 0.0053 0.0036 0.0125 0.0019
Bud 0 0 0 0 0 0 0.0007 0 0.0001 0.0001 0.0013 0 0.002 0 0.0001 0.0002 0.0011 0.0001
Bud Neck 0.0001 0.0001 0 0 0.0048 0.0005 0.0034 0 0.0001 0.0003 0.001 0.0007 0.0017 0.0001 0.0003 0.0005 0.0025 0.0026
Bud Periphery 0 0 0 0 0 0 0.0024 0 0.0001 0.0002 0.0019 0.0001 0.003 0.0001 0.0002 0.0006 0.0028 0.0002
Bud Site 0 0 0 0 0.0001 0 0.0029 0 0.0002 0.0005 0.0147 0.0001 0.0079 0.0001 0.0012 0.0008 0.0025 0.0003
Cell Periphery 0 0 0 0 0 0 0.0009 0 0 0.0001 0.0003 0 0.0004 0 0.0001 0.0003 0.0005 0.0001
Cytoplasm 0 0 0 0 0 0 0.0009 0 0.0001 0.01 0.0008 0.0001 0.0028 0.0002 0.0003 0.0029 0.0008 0.0004
Cytoplasmic Foci 0 0 0 0 0 0 0.0084 0 0.0025 0.0052 0.0034 0.0018 0.0119 0 0.0074 0.0093 0.0031 0.0019
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0.0002 0 0.0001 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0007 0 0.0001 0.002 0.002 0.0001 0.0015 0 0.0003 0.0024 0.0014 0.0007
Endosome 0 0 0 0 0 0 0.0132 0 0.0059 0.0152 0.0098 0.0008 0.0125 0.0001 0.0033 0.0343 0.0203 0.0089
Golgi 0 0 0 0 0 0 0.0053 0 0.0034 0.0129 0.0037 0.0009 0.0052 0 0.0026 0.0139 0.0139 0.0016
Lipid Particles 0 0 0 0 0 0 0.013 0 0.0095 0.0211 0.0305 0.0046 0.008 0 0.0116 0.028 0.0187 0.0023
Mitochondria 0.0001 0 0.0001 0 0.0002 0 0.0109 0 0.0092 0.0115 0.0203 0.0048 0.0058 0.0009 0.017 0.008 0.0355 0.0016
None 0 0 0 0 0 0 0.0012 0 0.0001 0.0019 0.0036 0.0001 0.0024 0 0.0002 0.0012 0.0006 0.0002
Nuclear Periphery 0.0002 0.0022 0.0007 0.0009 0.0006 0.0001 0.0073 0.0002 0.0051 0.005 0.0196 0.0008 0.0234 0.0009 0.0038 0.0181 0.0187 0.0042
Nucleolus 0.0394 0.0404 0.0214 0.0142 0.1613 0.1134 0.0565 0.0362 0.0222 0.0246 0.2143 0.1165 0.0421 0.0493 0.0279 0.0138 0.1717 0.1093
Nucleus 0.9599 0.9551 0.9764 0.9846 0.8271 0.8854 0.8476 0.9628 0.9344 0.8714 0.6439 0.8618 0.8361 0.9475 0.9114 0.848 0.6562 0.8566
Peroxisomes 0 0 0 0 0 0 0.0052 0 0.0024 0.0029 0.0041 0.001 0.0163 0 0.0048 0.0028 0.0038 0.0008
Punctate Nuclear 0.0002 0.0019 0.0004 0.0001 0.0056 0.0004 0.008 0.0006 0.001 0.0029 0.0161 0.0032 0.0068 0.0005 0.0016 0.0053 0.016 0.0054
Vacuole 0 0 0.0005 0 0.0001 0 0.0045 0 0.0004 0.0033 0.0021 0.0002 0.0028 0.0002 0.0004 0.0041 0.0088 0.0005
Vacuole Periphery 0 0 0.0004 0 0 0 0.0017 0 0.0003 0.0038 0.0027 0.0002 0.0016 0.0002 0.0004 0.0021 0.0085 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9572 12.1602 12.8409 16.6159 15.9409 14.6412 19.0338 19.948 20.9087 21.1674
Translational Efficiency 1.0396 1.2539 0.8496 0.8193 1.0254 1.0351 0.8507 0.7628 0.8504 0.8288

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of chromatin modifying histone acetyltransferase complexes; member of the ADA complex, the SAGA complex, and the SLIK complex; transcriptional regulator involved in glucose repression of Gal4p-regulated genes
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ngg1

Ngg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ngg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available