Standard name
Human Ortholog
Description Subunit of cohesin loading factor (Scc2p-Scc4p); a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; promotes gene expression program that supports translational fidelity; evolutionarily-conserved adherin; relocalizes to cytosol in response to hypoxia; human disorder Cornelia de Lange syndrome is caused by mutations in NIPBL, the human ortholog of SCC2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0.07 0 0 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.08 0.11 0.09 0.08 0.09 0.06 0.08 0.1 0.13 0.16 0.06 0.1 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.18 0.56 0.69 0.44 0.64 0.71 0.73 0.85 0.81 0.82 0.84 0.08 0.05 0.15 0.06 0 0 0 0 0 0 0 0
Nucleus 0.96 0.89 0.82 0.79 0.73 0.67 0.68 0.62 0.52 0.57 0.57 0.59 0.87 0.81 0.76 0.82 0.84 0.84 0.9 0.86 0.76 0.86 0.63 0.53
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0 0.07 0.11 0.1 0.25 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud 0 1 0 2 7 4 7 14 4 3 5 5 0 0 0 1 1 0 0 2 1 1 2 4
Bud Neck 0 0 0 0 0 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 1 4 8 8 18 27 4 8 10 15 0 0 3 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 3 9 3 9 13 19 38 38 16 26 10 19 21 46 54 6 6 4 0 2 2 0 3 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 0 0 0 1 0 0 2
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 1 0 0 2 2 1 0 5 5
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0
Mitochondria 20 61 79 138 111 150 245 301 176 246 142 194 17 18 49 6 2 2 0 4 4 0 4 9
Nucleus 216 301 116 157 183 158 237 255 108 174 99 136 177 276 252 78 49 49 218 296 112 145 157 170
Nuclear Periphery 1 2 2 1 4 0 3 1 0 8 0 1 2 2 5 1 0 0 0 0 1 0 0 4
Nucleolus 8 0 1 2 8 11 14 21 9 9 11 9 3 10 8 4 0 1 6 3 3 2 4 14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 4 2 2 11 2 0 1 2 0 4 2 0 0 0 2 2 0 0 3 4
Vac/Vac Membrane 4 13 1 1 8 4 3 5 1 13 3 8 1 12 11 4 5 6 7 25 16 17 62 91
Unique Cell Count 225 339 142 200 252 235 347 411 206 303 173 230 204 341 331 95 58 58 242 347 149 170 250 323
Labelled Cell Count 253 387 204 314 348 357 567 676 320 487 281 389 221 371 387 103 64 63 242 347 149 170 250 323


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.7 3.6 3.6 3.9 3.7 3.4 3.4 2.9 3.0 3.1 2.8 5.3 5.4 4.7 8.2 8.8 9.6 5.2 5.2 5.4
Std Deviation (1e-4) 1.0 1.1 1.0 1.0 1.3 1.6 1.4 1.6 1.2 1.2 1.6 0.7 1.4 1.3 1.5 2.2 2.3 2.2 1.2 1.4 1.3
Intensity Change (Log2) – – – -0.01 0.12 0.04 -0.11 -0.07 -0.31 -0.25 -0.2 -0.37 0.56 0.58 0.37 1.18 1.29 1.42 0.52 0.52 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9458 1.0871 1.1372 0.6258 0.955 1.1331 2.1151 2.5196 2.2294 2.0172 1.5144 1.8361 0.7839 0.8316 1.148 0.908 0.7756 0.9672
Actin 0.0406 0.0002 0.0002 0.018 0.0323 0.0046 0.0367 0.0002 0.0077 0.0021 0.0024 0 0.0031 0.0001 0.0002 0.0005 0.001 0
Bud 0.0004 0 0 0.0003 0.0005 0.0001 0.0007 0.0001 0.0004 0.001 0 0 0.0003 0 0 0.0002 0 0
Bud Neck 0.0096 0.0002 0.0007 0.0015 0.0019 0.0021 0.0062 0.0005 0.001 0.0009 0.0003 0.0014 0.0043 0.0001 0.0002 0.0006 0.0004 0.0023
Bud Periphery 0.0008 0 0.0001 0.0004 0.0008 0.0001 0.0015 0.0001 0.0008 0.0033 0.0001 0.0001 0.0023 0 0 0.0004 0 0.0001
Bud Site 0.003 0.0002 0.0005 0.0025 0.0035 0.0002 0.0131 0.0036 0.0018 0.0017 0.0002 0.0001 0.0009 0.0002 0.0002 0.0009 0.0001 0.0001
Cell Periphery 0.0003 0 0 0.0001 0.0003 0 0.0006 0.0001 0.0002 0.0004 0 0 0.0033 0 0 0.0002 0 0
Cytoplasm 0.0134 0.0064 0.0033 0.0072 0.0408 0.0013 0.0043 0.0049 0.0018 0.0014 0.0004 0.0003 0.0089 0.0067 0.0056 0.0017 0.002 0.0029
Cytoplasmic Foci 0.0077 0.0002 0.0014 0.0029 0.0133 0.0025 0.0171 0.0006 0.0014 0.0021 0.0177 0 0.0003 0.0005 0.0001 0.0001 0.011 0.0005
Eisosomes 0.0005 0.0001 0 0.0003 0.0005 0 0.0004 0 0.0001 0.0001 0 0 0.0001 0.0001 0.0002 0 0 0
Endoplasmic Reticulum 0.003 0.0004 0.0002 0.0009 0.0043 0.0001 0.0086 0.0005 0.0002 0.0005 0.0003 0 0.0301 0.0004 0.0005 0.0018 0.0002 0.0006
Endosome 0.0175 0.0008 0.0028 0.0041 0.0475 0.0031 0.0301 0.0009 0.0025 0.0149 0.0339 0 0.0026 0.0004 0.0005 0.0012 0.0073 0.0015
Golgi 0.0105 0 0.0004 0.004 0.0169 0.0043 0.0094 0.0002 0.0069 0.0059 0.01 0 0.0005 0 0 0.0001 0.0032 0.0001
Lipid Particles 0.0113 0.0001 0.0028 0.0063 0.0248 0.0029 0.0185 0.0002 0.0031 0.0049 0.0274 0 0.0006 0.0003 0.0001 0.0002 0.0144 0.0009
Mitochondria 0.0103 0.0003 0.0073 0.0048 0.0396 0.0009 0.0185 0.0005 0.0081 0.0309 0.0014 0.0001 0.0013 0.0002 0.0003 0.0012 0.0009 0.0001
None 0.0432 0.0407 0.0128 0.0238 0.0249 0.0005 0.003 0.0099 0.0008 0.0004 0.0003 0 0.0169 0.1248 0.0399 0.0009 0.0017 0.0013
Nuclear Periphery 0.0286 0.0099 0.0121 0.0968 0.0205 0.0092 0.0261 0.0202 0.0147 0.0049 0.001 0.0009 0.0126 0.0229 0.0191 0.0154 0.0043 0.0056
Nucleolus 0.0137 0.0085 0.009 0.0122 0.0085 0.0154 0.0132 0.0101 0.0096 0.0038 0.0083 0.0147 0.0101 0.0061 0.0081 0.0212 0.01 0.0221
Nucleus 0.7457 0.9177 0.938 0.7791 0.6835 0.933 0.7442 0.939 0.9265 0.9079 0.8653 0.9721 0.8763 0.8291 0.914 0.9471 0.9252 0.9477
Peroxisomes 0.0061 0 0.0004 0.0097 0.0208 0.0056 0.0225 0.0001 0.0048 0.0017 0.0236 0 0 0 0 0 0.0133 0.0001
Punctate Nuclear 0.0295 0.0135 0.0066 0.0235 0.0105 0.0137 0.0151 0.0065 0.0063 0.0013 0.0043 0.0099 0.0165 0.0075 0.0103 0.0045 0.0042 0.013
Vacuole 0.0029 0.0006 0.001 0.0006 0.0027 0.0003 0.0072 0.0015 0.0006 0.0075 0.0015 0.0002 0.0078 0.0003 0.0004 0.0015 0.0004 0.0008
Vacuole Periphery 0.0012 0.0002 0.0003 0.0012 0.0016 0.0001 0.003 0.0004 0.0006 0.0026 0.0018 0 0.0011 0.0001 0.0002 0.0005 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.5413 7.4826 10.6148 17.1301 15.1616 15.009 11.3484 10.7382 11.7593 20.8499
Translational Efficiency 0.656 0.8249 0.5839 0.4698 0.5652 1.0346 0.5503 0.481 0.5705 0.497

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of cohesin loading factor (Scc2p-Scc4p); a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; promotes gene expression program that supports translational fidelity; evolutionarily-conserved adherin; relocalizes to cytosol in response to hypoxia; human disorder Cornelia de Lange syndrome is caused by mutations in NIPBL, the human ortholog of SCC2
Localization
Cell Percentages nucleus (79%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Scc2

Scc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available