Standard name | |
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Human Ortholog | |
Description | Protein that interacts with CCT (chaperonin containing TCP-1) complex; has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 1 |
Bud | 0 | 1 | 0 | 0 | 1 | 3 | 5 | 11 | 1 | 7 | 0 | 5 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 2 | 7 | 0 | 4 | 1 | 1 | 1 | 5 | 1 | 5 | 2 | 0 | 1 | 8 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 182 | 170 | 186 | 158 | 254 | 287 | 323 | 354 | 178 | 264 | 185 | 278 | 115 | 114 | 115 | 139 | 154 | 139 | 182 | 171 | 170 | 116 | 163 | 157 |
Endoplasmic Reticulum | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 4 | 1 | 1 | 2 | 1 | 0 | 1 | 4 | 11 | 13 | 0 | 0 | 2 | 3 | 2 | 2 |
Endosome | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 3 | 2 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 3 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 4 | 3 | 3 | 4 | 9 | 68 | 125 | 164 | 107 | 135 | 120 | 126 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 3 | 4 | 4 | 1 |
Nucleus | 0 | 2 | 0 | 1 | 1 | 1 | 2 | 10 | 4 | 6 | 7 | 4 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 4 | 0 | 4 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 0 | 3 | 1 | 8 | 13 | 7 | 24 | 21 | 34 | 9 | 23 | 1 | 1 | 0 | 3 | 1 | 4 | 1 | 1 | 0 | 3 | 3 | 5 |
Unique Cell Count | 186 | 173 | 193 | 162 | 265 | 329 | 396 | 490 | 264 | 375 | 265 | 366 | 117 | 116 | 117 | 141 | 155 | 146 | 191 | 181 | 193 | 139 | 189 | 174 |
Labelled Cell Count | 189 | 177 | 197 | 173 | 276 | 377 | 465 | 571 | 318 | 458 | 325 | 449 | 120 | 117 | 118 | 157 | 169 | 163 | 191 | 181 | 193 | 139 | 189 | 174 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.0 | 5.7 | 5.8 | 5.7 | 4.7 | 4.7 | 5.1 | 4.9 | 4.9 | 4.7 | 4.7 | 5.7 | 5.7 | 6.4 | 6.0 | 6.0 | 6.0 | 5.0 | 5.9 | 6.0 |
Std Deviation (1e-4) | 0.7 | 1.3 | 1.6 | 6.4 | 2.1 | 1.2 | 1.8 | 1.5 | 1.6 | 1.6 | 1.5 | 1.2 | 0.7 | 0.8 | 1.0 | 1.3 | 1.0 | 1.2 | 1.0 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.0 | -0.29 | -0.27 | -0.15 | -0.23 | -0.22 | -0.27 | -0.27 | -0.0 | 0.01 | 0.17 | 0.08 | 0.07 | 0.07 | -0.2 | 0.04 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -0.3 | -3.5 | -4.9 | -7.0 | -7.6 | -7.2 | -7.2 | -6.1 | 1.0 | 1.0 | 1.0 | 1.2 | 1.8 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1.2 | 0 | 0 | 8.7 | 0 | 9.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 1.5 | 0 | 2.0 | 3.0 | 3.4 | 1.2 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.44 | 0.2051 | 0.0572 | -0.2395 | -0.3823 | 0.1993 | 1.0527 | 1.6091 | 1.5053 | 1.407 | 1.1749 | 1.6452 | 0.3371 | 0.9976 | 1.1258 | 0.8254 | 0.4252 | 0.7945 |
Actin | 0.0006 | 0.0005 | 0.0013 | 0.0007 | 0.0006 | 0.0022 | 0.0215 | 0.0085 | 0.0055 | 0.0154 | 0.0638 | 0.0196 | 0.005 | 0.0005 | 0.0104 | 0.0016 | 0 | 0.0008 |
Bud | 0 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0016 | 0.0165 | 0.0014 | 0.0003 | 0.0009 | 0.0057 | 0.0002 | 0.0003 | 0.0008 | 0.0059 | 0 | 0.0002 |
Bud Neck | 0.0001 | 0.0001 | 0.0006 | 0.0006 | 0.0002 | 0.0006 | 0.0108 | 0.0009 | 0.0009 | 0.0026 | 0.0278 | 0.0034 | 0.0001 | 0.0001 | 0.001 | 0.0003 | 0.0001 | 0.0012 |
Bud Periphery | 0 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0015 | 0.0058 | 0.001 | 0.0004 | 0.0011 | 0.0037 | 0.0004 | 0.0007 | 0.0008 | 0.0056 | 0 | 0.0001 |
Bud Site | 0.0002 | 0.0007 | 0.0024 | 0.0002 | 0.0001 | 0.0001 | 0.0052 | 0.0405 | 0.0022 | 0.0005 | 0.0007 | 0.0008 | 0.0011 | 0.0003 | 0.0054 | 0.0011 | 0 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 | 0 |
Cytoplasm | 0.406 | 0.5687 | 0.4995 | 0.5572 | 0.4804 | 0.5257 | 0.4457 | 0.5467 | 0.5736 | 0.4593 | 0.5097 | 0.6135 | 0.3506 | 0.5819 | 0.5928 | 0.6336 | 0.4247 | 0.6633 |
Cytoplasmic Foci | 0.0195 | 0.0274 | 0.0259 | 0.0201 | 0.0147 | 0.013 | 0.0322 | 0.0083 | 0.0286 | 0.0106 | 0.0623 | 0.0346 | 0.0101 | 0.0042 | 0.0234 | 0.0049 | 0.0011 | 0.005 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0043 | 0.0022 | 0.0039 | 0.0028 | 0.0029 | 0.0021 | 0.0071 | 0.0015 | 0.0021 | 0.0026 | 0.0024 | 0.0034 | 0.0021 | 0.0017 | 0.0029 | 0.0025 | 0.0003 | 0.0025 |
Endosome | 0.008 | 0.0061 | 0.0085 | 0.0099 | 0.007 | 0.0042 | 0.0147 | 0.002 | 0.0032 | 0.0077 | 0.0394 | 0.0093 | 0.0079 | 0.0008 | 0.0117 | 0.0039 | 0.0001 | 0.0014 |
Golgi | 0.0004 | 0.0007 | 0.0004 | 0.0005 | 0.0003 | 0.0002 | 0.004 | 0.0003 | 0.001 | 0.0059 | 0.0088 | 0.0032 | 0.0036 | 0.0002 | 0.0079 | 0.001 | 0 | 0.0001 |
Lipid Particles | 0.0008 | 0.0002 | 0.0006 | 0.0003 | 0.0004 | 0.0002 | 0.003 | 0.0002 | 0.0008 | 0.0037 | 0.0065 | 0.0023 | 0.0029 | 0.0001 | 0.0029 | 0.0001 | 0 | 0.0001 |
Mitochondria | 0.0003 | 0.0003 | 0.0008 | 0.0005 | 0.0006 | 0.0003 | 0.0065 | 0.0004 | 0.0008 | 0.005 | 0.0023 | 0.002 | 0.0084 | 0.0002 | 0.0127 | 0.0088 | 0 | 0.0001 |
None | 0.5534 | 0.3871 | 0.44 | 0.3953 | 0.4748 | 0.4432 | 0.4227 | 0.36 | 0.3654 | 0.4743 | 0.2486 | 0.276 | 0.593 | 0.4073 | 0.3075 | 0.3255 | 0.5733 | 0.3193 |
Nuclear Periphery | 0.0012 | 0.0008 | 0.0019 | 0.0012 | 0.0019 | 0.0007 | 0.0044 | 0.0007 | 0.0009 | 0.0027 | 0.0042 | 0.0053 | 0.0021 | 0.0003 | 0.0027 | 0.0008 | 0.0001 | 0.0007 |
Nucleolus | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0016 | 0.0003 | 0.0005 | 0.0004 | 0.0018 | 0.0004 | 0.0007 | 0.0005 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Nucleus | 0.0013 | 0.0019 | 0.0028 | 0.0067 | 0.0048 | 0.0028 | 0.0049 | 0.0029 | 0.0021 | 0.0029 | 0.0027 | 0.003 | 0.0018 | 0.0005 | 0.0018 | 0.0014 | 0.0002 | 0.0033 |
Peroxisomes | 0.0005 | 0.0009 | 0.0007 | 0.0005 | 0.0002 | 0.0008 | 0.0059 | 0.0003 | 0.0051 | 0.0007 | 0.0045 | 0.0049 | 0.0055 | 0.0001 | 0.0122 | 0.0002 | 0 | 0.0001 |
Punctate Nuclear | 0.0016 | 0.0009 | 0.0074 | 0.0013 | 0.0075 | 0.0026 | 0.0053 | 0.0028 | 0.0024 | 0.0024 | 0.0102 | 0.0067 | 0.0027 | 0.0003 | 0.001 | 0.0003 | 0.0001 | 0.0011 |
Vacuole | 0.001 | 0.001 | 0.0011 | 0.0014 | 0.001 | 0.0005 | 0.0016 | 0.0008 | 0.0008 | 0.0013 | 0.0022 | 0.001 | 0.0012 | 0.0004 | 0.0011 | 0.0017 | 0.0001 | 0.0004 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0006 | 0.0009 | 0.0005 | 0.0004 | 0 | 0.0003 | 0.0007 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.9027 | 11.3867 | 11.618 | 11.8013 | 12.3863 | 7.9567 | 12.5925 | 12.1415 | 10.1404 | 16.7607 |
Translational Efficiency | 1.856 | 1.5403 | 1.5004 | 1.4285 | 1.372 | 4.4752 | 1.638 | 1.387 | 1.9846 | 1.4185 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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