Standard name | |
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Human Ortholog | |
Description | Mitochondrial transcription elongation factor; DEAD-box protein; required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing; promotes RNA folding by stabilizing an early assembly intermediate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 2 | 3 | 10 | 1 | 3 | – | 6 | 2 | 4 | 3 | 4 | 13 | 4 | 14 | 11 | 9 | 1 | 6 | 2 | 3 | 10 | 22 |
Bud | 0 | 1 | 0 | 4 | 3 | 5 | 10 | – | 7 | 8 | 9 | 10 | 1 | 3 | 4 | 0 | 5 | 5 | 1 | 0 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 2 | 7 | 2 | 5 | 1 | 1 | 4 | – | 1 | 3 | 0 | 4 | 4 | 6 | 9 | 8 | 9 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 0 | 2 | 2 | 4 | 4 | 14 | – | 0 | 7 | 2 | 7 | 2 | 1 | 8 | 7 | 4 | 7 | 0 | 0 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 2 | 1 | 1 | 0 | 1 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 3 | 0 | 3 | 2 | 3 | – | 2 | 7 | 1 | 10 | 3 | 4 | 3 | 6 | 1 | 5 | 1 | 1 | 6 | 1 | 3 | 7 |
Golgi | 2 | 21 | 72 | 49 | 95 | 13 | 15 | – | 11 | 14 | 7 | 10 | 55 | 128 | 112 | 111 | 74 | 31 | 11 | 20 | 15 | 21 | 66 | 58 |
Mitochondria | 194 | 145 | 109 | 171 | 211 | 240 | 307 | – | 259 | 293 | 302 | 355 | 161 | 246 | 212 | 17 | 24 | 8 | 176 | 144 | 133 | 100 | 186 | 200 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 1 | 0 | – | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 9 | 3 | 1 | 6 | 3 | 11 | 25 | – | 19 | 20 | 15 | 19 | 3 | 4 | 7 | 3 | 10 | 3 | 0 | 0 | 0 | 0 | 1 | 3 |
Nucleolus | 21 | 4 | 9 | 10 | 27 | 22 | 41 | – | 30 | 28 | 35 | 31 | 4 | 6 | 9 | 11 | 16 | 13 | 1 | 0 | 0 | 0 | 1 | 2 |
Peroxisomes | 4 | 1 | 3 | 6 | 13 | 10 | 20 | – | 7 | 5 | 6 | 15 | 2 | 10 | 19 | 8 | 5 | 6 | 7 | 2 | 10 | 3 | 5 | 4 |
SpindlePole | 0 | 1 | 1 | 0 | 2 | 1 | 2 | – | 1 | 3 | 4 | 3 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 0 | 0 | 1 | 1 | 0 |
Vac/Vac Membrane | 9 | 25 | 27 | 9 | 17 | 8 | 13 | – | 17 | 13 | 8 | 16 | 19 | 15 | 18 | 16 | 35 | 18 | 1 | 5 | 2 | 5 | 10 | 19 |
Unique Cell Count | 207 | 168 | 153 | 200 | 271 | 267 | 383 | 293 | 343 | 327 | 404 | 181 | 283 | 285 | 170 | 163 | 96 | 209 | 185 | 175 | 138 | 292 | 327 | |
Labelled Cell Count | 245 | 212 | 231 | 265 | 389 | 320 | 458 | 362 | 407 | 397 | 486 | 258 | 437 | 406 | 208 | 197 | 118 | 209 | 185 | 175 | 138 | 292 | 327 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.3 | 22.7 | 16.3 | 17.4 | 18.3 | 17.1 | 15.7 | – | 13.9 | 15.3 | 13.0 | 13.4 | 18.5 | 18.5 | 17.8 | 22.1 | 22.8 | 21.1 | 16.7 | 18.4 | 19.4 |
Std Deviation (1e-4) | 3.7 | 4.6 | 3.3 | 4.3 | 4.1 | 5.3 | 4.0 | – | 4.0 | 4.7 | 3.7 | 3.8 | 3.8 | 3.6 | 3.7 | 6.4 | 6.9 | 6.3 | 3.4 | 4.2 | 4.6 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.17 | 0.07 | -0.05 | – | -0.23 | -0.09 | -0.32 | -0.28 | 0.18 | 0.18 | 0.13 | 0.44 | 0.49 | 0.37 | 0.04 | 0.18 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.8 | 0 | 2.9 | 2.4 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -4.4 | -2.4 | -10.4 | -12.2 | – | -11.2 | -11.7 | -12.4 | -13.3 | -3.1 | -0.4 | -1.6 | 3.3 | -0.3 | -2.3 |
Mitochondria | 3.3 | 1.5 | 4.9 | 2.2 | – | 4.5 | 3.7 | 6.1 | 4.7 | 4.1 | 4.0 | 0.7 | -11.3 | -10.2 | -9.7 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 2.9 | – | 2.8 | 2.6 | 2.2 | 2.3 | 0 | 0 | 0 | 0 | 2.7 | 0 |
Nucleolus | -0.4 | 1.4 | 0.9 | 1.7 | – | 1.5 | 0.9 | 1.7 | 0.7 | -1.7 | -2.1 | -1.4 | 0.2 | 1.3 | 2.1 |
Peroxisomes | 0 | 1.5 | 0 | 1.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 1.4 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.0 | -3.7 | -5.2 | -5.7 | – | -4.0 | -5.2 | -6.0 | -5.4 | -1.9 | -4.2 | -3.7 | -2.2 | 0.9 | 0.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 18.4332 | 19.8308 | 19.9968 | 18.2544 | 16.9516 | 19.0451 | 23.6214 | 23.3738 | 22.2494 | 22.7208 | 18.3989 | 22.974 | 21.4635 | 23.1207 | 22.952 | 21.1477 | 16.9689 | 21.8111 |
Actin | 0.0244 | 0.0034 | 0.011 | 0.0004 | 0.0201 | 0.0009 | 0.0105 | 0.005 | 0.0089 | 0.0047 | 0.0323 | 0.0009 | 0.0177 | 0.0088 | 0.0089 | 0.0002 | 0.0174 | 0.0005 |
Bud | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0016 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0045 | 0 |
Bud Neck | 0.001 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0003 | 0 |
Bud Periphery | 0.0005 | 0.0006 | 0.0002 | 0 | 0.0004 | 0 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0035 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0196 | 0 |
Bud Site | 0.0034 | 0.0002 | 0.0011 | 0 | 0.0008 | 0 | 0.0005 | 0.0004 | 0.0002 | 0 | 0.0002 | 0 | 0.0014 | 0.0003 | 0.0048 | 0 | 0.0045 | 0.0002 |
Cell Periphery | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0005 | 0.0002 | 0 | 0.0005 | 0.0001 |
Cytoplasm | 0.0023 | 0 | 0.0037 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0025 | 0 | 0.0006 | 0 | 0.0003 | 0.0002 |
Cytoplasmic Foci | 0.0142 | 0.0007 | 0.0033 | 0.0001 | 0.0068 | 0.0013 | 0.0055 | 0.0004 | 0.0021 | 0.0002 | 0.0068 | 0 | 0.0194 | 0.0003 | 0.0007 | 0 | 0.0022 | 0.0105 |
Eisosomes | 0.0009 | 0.0003 | 0.0017 | 0.0002 | 0.0007 | 0.0002 | 0.001 | 0.0006 | 0.0013 | 0.0008 | 0.0004 | 0.0005 | 0.0009 | 0.0005 | 0.0004 | 0.0001 | 0.0007 | 0.0001 |
Endoplasmic Reticulum | 0.0017 | 0.0001 | 0.0006 | 0 | 0.0005 | 0 | 0.0004 | 0.0002 | 0.0005 | 0 | 0 | 0 | 0.0007 | 0 | 0.0023 | 0 | 0.0008 | 0.0001 |
Endosome | 0.0172 | 0.0011 | 0.0047 | 0.0002 | 0.0052 | 0.0008 | 0.0044 | 0.0023 | 0.0028 | 0.0001 | 0.0102 | 0.0003 | 0.0054 | 0.0009 | 0.0012 | 0 | 0.0072 | 0.0026 |
Golgi | 0.0159 | 0.0037 | 0.006 | 0.0011 | 0.02 | 0.0083 | 0.0098 | 0.0093 | 0.0053 | 0.0006 | 0.0115 | 0.0103 | 0.0094 | 0.0132 | 0.0021 | 0.0002 | 0.0053 | 0.0036 |
Lipid Particles | 0.0577 | 0.0198 | 0.0143 | 0.001 | 0.0237 | 0.0173 | 0.0203 | 0.0014 | 0.0079 | 0.0113 | 0.0143 | 0.0003 | 0.0235 | 0.0103 | 0.0072 | 0.0004 | 0.0139 | 0.0098 |
Mitochondria | 0.8331 | 0.9514 | 0.9299 | 0.9944 | 0.9043 | 0.9633 | 0.9228 | 0.9709 | 0.9456 | 0.9733 | 0.9068 | 0.986 | 0.8817 | 0.9505 | 0.9627 | 0.9979 | 0.9025 | 0.955 |
None | 0.0007 | 0 | 0.0001 | 0 | 0.0011 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0 | 0 | 0.0053 | 0 | 0.0005 | 0 | 0.0009 | 0.0004 |
Nuclear Periphery | 0.0015 | 0.0001 | 0.002 | 0 | 0.0017 | 0 | 0.0029 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0.0028 | 0 | 0.0009 | 0 | 0.0028 | 0.0023 |
Nucleolus | 0.0011 | 0.0004 | 0.0002 | 0 | 0.0005 | 0.0012 | 0.0056 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0057 | 0.0002 | 0.0001 | 0 | 0.0016 | 0.0004 |
Nucleus | 0.0011 | 0.0002 | 0.0006 | 0 | 0.0004 | 0.0001 | 0.0013 | 0.0002 | 0 | 0 | 0 | 0 | 0.0011 | 0.0001 | 0.0002 | 0 | 0.0008 | 0.0003 |
Peroxisomes | 0.0149 | 0.0062 | 0.0075 | 0.002 | 0.0077 | 0.0021 | 0.0077 | 0.0024 | 0.017 | 0.0053 | 0.0101 | 0.001 | 0.0094 | 0.0022 | 0.002 | 0.0007 | 0.0051 | 0.0024 |
Punctate Nuclear | 0.0016 | 0 | 0.0005 | 0 | 0.0031 | 0.0001 | 0.0017 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0043 | 0 | 0.0003 | 0 | 0.001 | 0.0005 |
Vacuole | 0.002 | 0.0034 | 0.0015 | 0.0001 | 0.0004 | 0.0002 | 0.0007 | 0.0025 | 0.0011 | 0.0002 | 0.0004 | 0 | 0.001 | 0.0015 | 0.0004 | 0 | 0.0028 | 0.0004 |
Vacuole Periphery | 0.0041 | 0.0077 | 0.0103 | 0.0004 | 0.0018 | 0.0042 | 0.0029 | 0.003 | 0.0064 | 0.0032 | 0.0013 | 0.0006 | 0.0071 | 0.0102 | 0.0036 | 0.0004 | 0.0054 | 0.0106 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 79.0911 | 62.2974 | 66.406 | 70.9322 | 58.085 | 64.1223 | 81.7356 | 74.9043 | 69.3046 | 86.744 |
Translational Efficiency | 0.712 | 0.8838 | 0.7479 | 0.6519 | 0.7768 | 0.7957 | 0.7588 | 0.6863 | 0.7752 | 0.7088 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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