Standard name
Human Ortholog
Description Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance; VPS64 has a paralog, FAR10, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0.05 0 0 0
Bud 0 0 0.06 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0.05 0 0 0.06 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0.07 0 0 0.05 0 0 – 0 0 0 0 – – – 0.16 0.16 0.19 0 0 0.06 0 0 0
Cytoplasm 0.84 0.88 0.9 0.95 0.95 0.83 0.85 – 0.73 0.76 0.62 0.62 – – – 0.84 0.82 0.86 0.53 0.57 0.41 0.29 0.21 0.16
Endoplasmic Reticulum 0.13 0.07 0.06 0 0 0.13 0.06 – 0.06 0.05 0 0.07 – – – 0.08 0.06 0 0.25 0.19 0.15 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.12
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.06 0
Mitochondria 0 0 0 0.19 0 0.05 0.07 – 0.31 0.2 0.58 0.43 – – – 0 0.06 0 0.08 0.07 0.18 0.13 0 0
Nucleus 0 0 0 0.05 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.06
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0.05 0.06 0 0 0 0.06 0.39 0.26 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 8 0 0 2 1 0 – 0 2 0 1 – – – 3 3 3 11 11 3 0 0 0
Bud 2 4 3 0 0 0 0 – 2 1 1 2 – – – 0 2 0 2 4 0 0 0 0
Bud Neck 0 0 0 0 1 0 0 – 1 0 0 0 – – – 2 2 0 9 16 3 0 1 0
Bud Site 0 0 0 0 0 0 0 – 0 1 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 12 21 2 0 6 4 7 – 1 2 0 2 – – – 35 26 23 7 8 4 0 0 0
Cytoplasm 239 274 47 20 105 99 134 – 80 57 56 80 – – – 179 130 102 181 201 33 4 3 3
Endoplasmic Reticulum 37 21 3 0 2 15 10 – 7 4 2 9 – – – 16 10 3 86 68 11 0 0 0
Endosome 0 2 0 0 0 1 1 – 2 0 0 0 – – – 4 5 1 4 2 0 0 0 2
Golgi 0 2 0 0 0 0 0 – 0 0 0 0 – – – 2 1 1 2 4 0 0 1 0
Mitochondria 10 4 0 4 1 6 11 – 34 15 53 55 – – – 7 9 0 26 23 14 2 0 0
Nucleus 0 1 1 1 0 0 1 – 1 1 1 1 – – – 3 1 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 1 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 1 0 – – – 3 0 3 1 0 0 0 0 0
Vac/Vac Membrane 0 8 0 0 0 1 2 – 2 1 0 3 – – – 11 10 3 5 6 4 6 4 3
Unique Cell Count 284 312 52 21 110 119 157 109 75 91 129 213 159 119 343 353 81 17 18 19
Labelled Cell Count 304 345 56 25 117 127 167 130 84 114 153 265 199 139 343 353 81 17 18 19


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 6.4 5.7 5.2 6.2 5.6 5.4 – 4.8 5.0 4.6 4.5 – – – 7.4 7.9 7.7 – – –
Std Deviation (1e-4) 0.5 1.1 0.8 0.5 1.0 1.0 1.3 – 1.6 0.8 1.7 0.8 – – – 1.2 1.1 1.7 – – –
Intensity Change (Log2) – – – -0.12 0.13 -0.01 -0.06 – -0.25 -0.18 -0.3 -0.33 – – – 0.39 0.48 0.45 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 2.3 2.3 2.6
Cytoplasm 0.7 1.3 -1.2 -0.9 – -2.5 -2.1 -3.7 -3.8 – – – -1.2 -1.5 -0.8
Endoplasmic Reticulum 0 0 1.3 0 – 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 – 0 3.4 0 0 – – – 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5092 0.8079 0.6125 0.4949 0.3822 0.7349 0.8993 1.1048 1.0537 1.2166 1.7009 1.0145 0.4796 0.6752 0.4739 0.7501 0.5764 0.6435
Actin 0.0262 0.0009 0.0087 0.0021 0.0044 0.0084 0.0281 0.0019 0.0119 0.035 0.0332 0.0059 0.0097 0.0046 0.0086 0.0019 0.0007 0.0038
Bud 0.005 0.0053 0.0018 0.0149 0.0151 0.0016 0.0015 0.0014 0.0046 0.0008 0.0048 0.0004 0.0013 0.0038 0.0024 0.0006 0.0007 0.0002
Bud Neck 0.0044 0.0008 0.0025 0.0014 0.0012 0.0077 0.0029 0.0011 0.0042 0.0038 0.0137 0.0023 0.0013 0.0003 0.0011 0.0006 0.0008 0.0022
Bud Periphery 0.0049 0.0023 0.0047 0.0097 0.0069 0.0026 0.0056 0.0053 0.0105 0.0018 0.0097 0.0013 0.0014 0.0025 0.0083 0.0014 0.0011 0.0004
Bud Site 0.0068 0.0182 0.0088 0.0011 0.0243 0.0044 0.0096 0.0319 0.0305 0.0107 0.0375 0.0009 0.0062 0.0079 0.0043 0.0006 0.001 0.0002
Cell Periphery 0.0016 0.0013 0.0029 0.0008 0.0007 0.0007 0.0014 0.0022 0.0021 0.0016 0.0009 0.0005 0.0009 0.0008 0.0013 0.0008 0.0003 0.0003
Cytoplasm 0.4216 0.5743 0.4544 0.5583 0.4972 0.5274 0.2998 0.4679 0.4211 0.4617 0.2752 0.4706 0.5368 0.6696 0.6819 0.7078 0.5834 0.6296
Cytoplasmic Foci 0.0297 0.02 0.0253 0.017 0.0293 0.0366 0.0316 0.0161 0.0192 0.0224 0.0693 0.0113 0.0169 0.0115 0.0109 0.0067 0.0121 0.0171
Eisosomes 0.0006 0.0003 0.001 0.0001 0.0001 0.0002 0.0005 0.0003 0.0004 0.0007 0.0002 0.0001 0.0003 0.0001 0.0002 0.0001 0 0.0001
Endoplasmic Reticulum 0.0206 0.0086 0.013 0.0116 0.0067 0.0137 0.0139 0.0109 0.0163 0.0432 0.0041 0.0101 0.0386 0.0124 0.0247 0.0219 0.0029 0.009
Endosome 0.0354 0.0121 0.0179 0.0173 0.0291 0.0292 0.0325 0.0097 0.0151 0.0271 0.0374 0.0113 0.0196 0.008 0.009 0.0057 0.0221 0.0066
Golgi 0.008 0.0013 0.0118 0.002 0.0106 0.004 0.0103 0.0013 0.0033 0.0039 0.0166 0.0027 0.0027 0.0007 0.0018 0.0006 0.001 0.0011
Lipid Particles 0.0218 0.0013 0.0143 0.0006 0.0155 0.0071 0.0171 0.0019 0.0035 0.0095 0.0128 0.0046 0.0084 0.001 0.0021 0.0003 0.0006 0.0006
Mitochondria 0.0211 0.0008 0.0094 0.0044 0.0057 0.0049 0.0167 0.0013 0.0038 0.0018 0.0055 0.0076 0.0011 0.0018 0.001 0.0006 0.0005 0.0016
None 0.3507 0.342 0.3972 0.3468 0.3409 0.3363 0.4709 0.434 0.4351 0.3463 0.3664 0.457 0.3402 0.2691 0.2259 0.2442 0.3673 0.3223
Nuclear Periphery 0.0083 0.001 0.0022 0.0016 0.0014 0.002 0.0135 0.0014 0.0022 0.0033 0.0066 0.0034 0.0036 0.0008 0.0046 0.0014 0.0004 0.0008
Nucleolus 0.0014 0.0006 0.001 0.0003 0.0004 0.0005 0.0025 0.0009 0.001 0.0025 0.0027 0.0013 0.0005 0.0003 0.0008 0.0001 0.0001 0.0001
Nucleus 0.0029 0.002 0.0023 0.002 0.0016 0.0021 0.0096 0.0024 0.0034 0.0034 0.0065 0.0022 0.0023 0.0021 0.0024 0.0015 0.0011 0.0009
Peroxisomes 0.0166 0.0002 0.0109 0.0003 0.0023 0.0032 0.0144 0.0016 0.0035 0.0025 0.0691 0.0016 0.001 0.0001 0.0012 0.0001 0.0002 0.0009
Punctate Nuclear 0.0053 0.0028 0.0046 0.0015 0.0017 0.0034 0.0108 0.0014 0.0023 0.0123 0.0125 0.0031 0.0031 0.0005 0.004 0.0005 0.0006 0.0009
Vacuole 0.0055 0.0036 0.0045 0.0054 0.0037 0.0032 0.0047 0.0048 0.0054 0.0049 0.014 0.0015 0.0034 0.002 0.0029 0.0024 0.0024 0.0011
Vacuole Periphery 0.0016 0.0003 0.0008 0.0008 0.001 0.0007 0.0021 0.0004 0.0007 0.0006 0.0015 0.0004 0.0005 0.0002 0.0005 0.0003 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.9429 11.9561 6.4523 11.9886 15.3702 11.5444 12.3635 9.6426 12.0994 16.5483
Translational Efficiency 1.2826 0.9162 1.1665 0.7387 0.6584 1.3601 0.8852 1.2973 0.955 0.7011

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance; VPS64 has a paralog, FAR10, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (35%), ER (8%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vps64

Vps64


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps64-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available