Standard name
Human Ortholog
Description Protein with a role in ubiquinone (Coenzyme Q) biosynthesis; possibly functioning in stabilization of Coq7p; located on matrix face of mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex; human homolog COQ4 can complement yeast coq4 null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud 0.07 0.08 0.08 0 0.07 0.07 0.11 0.1 0.11 0.09 0.08 0.1 0 0 0 0.06 0.05 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.09 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.37 0 0.36 0.51 0.46 0.26 0.13 0.08 0.06 0.06 0.06 0.05 0.61 0.61 0.51 0 0.05 0.07 0.1 0 0 0.14 0.15 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.2 0 0 0 0.07 0 0 0 0 0 0 0.06 0.08 0.16 0.28 0.19 0.21 0.22 0.26 0.22 0.15 0.18 0.14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Mitochondria 0.52 0.46 0.4 0.18 0.14 0.51 0.79 0.71 0.87 0.84 0.92 0.89 0 0 0 0.11 0.06 0.09 0.35 0.27 0.37 0.28 0.26 0.27
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.26 0 0 0 0.12 0 0 0 0 0 0 0 0.05 0.2 0.43 0.47 0.56 0.06 0.25 0.09 0.07 0.08 0.08
SpindlePole 0.18 0.29 0.26 0.33 0.3 0.24 0.21 0.27 0.16 0.17 0.08 0.14 0.26 0.25 0.24 0.46 0.28 0.3 0.14 0.11 0.13 0.22 0.19 0.21
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 4 2 1 1 1 5 0 1 0 0 7 3 5 5 6 2 11 4 3 8 19 22
Bud 18 8 4 4 11 13 24 19 28 26 20 17 2 8 6 23 9 21 1 0 0 1 9 8
Bud Neck 6 0 2 4 13 3 1 6 2 3 1 3 10 3 9 2 0 1 4 0 0 6 7 10
Bud Site 3 2 0 1 1 4 9 3 6 3 6 3 1 0 0 6 4 10 – – – – – –
Cell Periphery 0 1 0 0 4 0 1 0 0 0 1 0 0 3 1 5 2 4 1 0 0 1 1 2
Cytoplasm 94 3 19 45 70 47 27 15 14 16 14 9 128 173 151 10 8 20 30 0 5 34 68 45
Endoplasmic Reticulum 2 2 2 1 2 0 0 1 1 1 1 1 2 1 2 5 0 2 1 0 0 1 3 1
Endosome 1 20 0 1 4 12 1 3 1 3 0 1 13 24 47 106 32 61 69 30 28 36 81 53
Golgi 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 8 10 6 2 2 2 2 2 4
Mitochondria 131 47 21 16 21 91 166 140 217 233 233 154 8 8 4 41 10 25 108 32 46 67 120 104
Nucleus 0 0 0 1 0 1 4 2 1 5 4 7 3 0 2 3 1 3 0 0 0 1 0 0
Nuclear Periphery 0 0 0 1 0 0 0 2 1 5 3 1 0 0 1 0 0 0 0 1 1 0 0 0
Nucleolus 0 2 0 0 0 2 4 4 6 5 6 3 0 0 0 2 3 8 2 0 1 1 1 4
Peroxisomes 5 27 1 0 4 21 7 6 4 5 1 3 6 14 58 163 78 161 17 29 11 17 36 30
SpindlePole 46 30 14 29 45 43 44 53 40 46 20 24 54 70 71 177 47 85 42 12 15 53 87 81
Vac/Vac Membrane 4 4 0 0 2 7 2 1 4 2 8 1 0 4 2 14 8 8 6 2 1 5 10 4
Unique Cell Count 254 102 53 89 151 180 210 197 249 278 253 174 211 284 297 382 165 287 313 118 127 246 468 384
Labelled Cell Count 311 148 67 105 179 245 291 260 325 354 318 227 234 311 359 570 218 417 313 118 127 246 468 384


Ambiguous, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 4.8 4.2 4.4 4.6 3.9 3.8 4.2 3.6 3.9 3.7 3.7 4.7 5.0 5.1 6.4 6.9 6.2 4.5 4.6 5.3
Std Deviation (1e-4) 0.6 1.2 1.4 1.6 1.2 1.6 1.4 1.0 1.2 1.3 1.2 0.7 1.0 1.5 1.5 1.4 1.5 1.7 1.7 1.3 2.2
Intensity Change (Log2) – – – 0.07 0.14 -0.09 -0.14 0.0 -0.2 -0.11 -0.19 -0.17 0.17 0.27 0.3 0.62 0.72 0.58 0.1 0.13 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1426 -0.4464 -0.5009 -0.6416 -1.0076 -0.304 -0.5391 -0.5184 -0.369 -0.1728 -0.2239 -0.4078 -0.8324 -0.9482 -0.9912 -0.4549 -0.9879 -0.8662
Actin 0.0341 0.0051 0.0079 0.0051 0.0514 0.0054 0.0465 0.0087 0.0153 0.0231 0.0119 0.0269 0.0363 0.0146 0.0124 0.0383 0.041 0.0314
Bud 0.0004 0.0043 0.0006 0.0002 0.0002 0.0002 0.0015 0.0024 0.0006 0.0007 0.0005 0.0002 0.0008 0.0003 0.0012 0.0036 0.0009 0.001
Bud Neck 0.0011 0.0006 0.001 0.0008 0.0005 0.0201 0.0054 0.0011 0.0089 0.0287 0.0008 0.0066 0.0018 0.0007 0.011 0.0013 0.0014 0.0106
Bud Periphery 0.0008 0.002 0.0023 0.0006 0.0005 0.0004 0.003 0.0054 0.0021 0.0009 0.0009 0.0006 0.0015 0.0003 0.0025 0.0071 0.0009 0.0016
Bud Site 0.0016 0.0104 0.003 0.0046 0.0004 0.0006 0.01 0.0191 0.0105 0.0082 0.0053 0.0005 0.0034 0.0163 0.0068 0.0039 0.0049 0.0022
Cell Periphery 0.0003 0.0003 0.0002 0.0003 0.0001 0.0001 0.0009 0.0003 0.0004 0.0007 0.0001 0.0002 0.0004 0.0001 0.0003 0.0005 0.0001 0.0002
Cytoplasm 0.0088 0.0135 0.0185 0.0085 0.0026 0.0091 0.0079 0.017 0.0169 0.0268 0.0052 0.0343 0.014 0.0089 0.0119 0.012 0.0014 0.0031
Cytoplasmic Foci 0.2513 0.2022 0.2132 0.1509 0.1365 0.217 0.273 0.2878 0.2653 0.225 0.1557 0.2783 0.235 0.2733 0.2488 0.1952 0.2348 0.2888
Eisosomes 0.0006 0.0002 0.0002 0.0004 0.0008 0.0006 0.0005 0.0003 0.0007 0.0008 0.0003 0.0004 0.0006 0.0001 0.0002 0.0005 0.0001 0.0001
Endoplasmic Reticulum 0.0039 0.0014 0.0008 0.0003 0.0001 0.0009 0.0012 0.0005 0.0021 0.0059 0.0003 0.0015 0.0013 0.0003 0.001 0.0016 0.0003 0.0007
Endosome 0.0465 0.0641 0.0481 0.0184 0.0273 0.0223 0.0822 0.055 0.0774 0.0411 0.0465 0.0352 0.0569 0.054 0.0545 0.0626 0.1029 0.0286
Golgi 0.0227 0.0271 0.0206 0.0203 0.0103 0.0257 0.0238 0.0234 0.0296 0.0395 0.0272 0.0143 0.0301 0.0403 0.0365 0.0603 0.1065 0.0334
Lipid Particles 0.1198 0.0834 0.0998 0.0826 0.05 0.0964 0.1186 0.059 0.0627 0.0436 0.0454 0.0343 0.106 0.0686 0.1018 0.0526 0.0987 0.1039
Mitochondria 0.0124 0.036 0.0507 0.0581 0.0085 0.0158 0.0382 0.0488 0.0633 0.0744 0.0192 0.0691 0.0374 0.0358 0.0577 0.0646 0.0577 0.014
None 0.0713 0.0552 0.0685 0.126 0.1733 0.1614 0.1276 0.117 0.1316 0.1436 0.1523 0.1096 0.0874 0.028 0.0522 0.083 0.0005 0.006
Nuclear Periphery 0.0105 0.0007 0.0006 0.0001 0 0.0003 0.0013 0.0003 0.0006 0.0013 0.0005 0.0007 0.0009 0.0002 0.0009 0.002 0.0001 0.0005
Nucleolus 0.0015 0.0012 0.0011 0.0007 0.0001 0.0021 0.004 0.0008 0.0008 0.0007 0.0007 0.0003 0.0007 0.0006 0.001 0.0032 0.0002 0.0003
Nucleus 0.0079 0.0006 0.0036 0.0004 0.0001 0.0007 0.0018 0.0005 0.0006 0.0012 0.0003 0.0004 0.0005 0.0002 0.0011 0.0049 0.0001 0.0005
Peroxisomes 0.3799 0.4764 0.446 0.498 0.5362 0.4092 0.2238 0.343 0.2999 0.3274 0.5208 0.3744 0.3688 0.4497 0.3889 0.3574 0.3435 0.4517
Punctate Nuclear 0.0169 0.011 0.0099 0.0228 0.0007 0.0104 0.0063 0.0071 0.0063 0.003 0.0046 0.01 0.0059 0.0047 0.0048 0.0397 0.0014 0.0193
Vacuole 0.0057 0.0032 0.0024 0.0006 0.0004 0.001 0.0174 0.0019 0.0031 0.0027 0.001 0.0015 0.0084 0.0023 0.0034 0.0036 0.0018 0.0017
Vacuole Periphery 0.0022 0.0011 0.0009 0.0003 0.0001 0.0003 0.0052 0.0005 0.0013 0.0007 0.0005 0.0007 0.002 0.0006 0.0011 0.0022 0.0008 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.609 16.6353 17.427 24.9303 24.1274 30.6332 24.4879 21.703 28.4091 23.5116
Translational Efficiency 0.6975 0.7095 1.0299 0.8941 0.6929 0.5996 0.6463 1.0896 0.9186 0.8556

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Protein with a role in ubiquinone (Coenzyme Q) biosynthesis; possibly functioning in stabilization of Coq7p; located on matrix face of mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex; human homolog COQ4 can complement yeast coq4 null mutant
Localization
Cell Percentages mitochondrion (40%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Coq4

Coq4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Coq4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available