Standard name
Human Ortholog
Description Sterol regulatory element binding protein; induces sterol biosynthetic genes, upon sterol depletion; acts as a sterol sensor, binding ergosterol in sterol rich conditions; relocates from intracellular membranes to perinuclear foci upon sterol depletion; redundant activator of filamentation with ECM22, up-regulating the expression of filamentous growth genes; contains a Zn[2]-Cys[6] binuclear cluster; UPC2 has a paralog, ECM22, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.14 0.15 0.37 0.26 0.26 0.29 0.37 0.34 0.25 0.21 0.25 0.17 0.19 0.37 0.54 0.29 0.23 0.18 0.05 0.1 0.09 0.15 0.17 0.23
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.06 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.32 0.07 0.07 0 0.32 0.39 0.31 0.7 0.67 0.69 0.61 0.08 0 0 0.07 0 0.1 0 0.05 0.09 0.09 0.12 0.1
Nucleus 0.58 0.56 0.45 0.62 0.6 0.34 0.27 0.34 0.26 0.35 0.25 0.38 0.64 0.36 0.24 0.5 0.54 0.52 0.48 0.47 0.33 0.19 0.12 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0.07 0.07 0 0 0.06 0 0.06 0 0.09 0 0 0 0 0 0.05 0.09 0.05 0.07 0.05 0.07 0.06
Vac/Vac Membrane 0.12 0.08 0.09 0 0.08 0.12 0.06 0.06 0 0 0 0 0.12 0.27 0.22 0.16 0.17 0.2 0.17 0.23 0.29 0.37 0.35 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 1 2 6
Bud 0 1 0 0 0 1 2 5 3 5 6 5 0 0 0 0 0 0 4 4 0 8 16 20
Bud Neck 0 5 6 2 8 3 5 8 0 4 1 1 0 0 1 0 0 1 1 2 1 7 13 15
Bud Site 0 2 0 1 0 2 3 6 11 9 2 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 31 54 44 22 42 45 83 68 62 41 38 27 50 89 150 93 57 38 14 47 21 44 83 117
Endoplasmic Reticulum 1 2 2 0 2 0 0 0 0 0 2 0 1 1 0 16 14 11 0 1 0 4 7 8
Endosome 10 5 2 0 0 2 7 4 0 0 0 0 9 10 6 15 19 12 16 10 4 5 11 5
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 4 0 0 0 2 1
Mitochondria 47 118 8 6 6 50 88 62 173 132 106 98 21 2 6 21 8 20 3 23 20 26 62 52
Nucleus 132 208 53 52 98 53 60 69 64 68 39 61 170 85 66 160 134 110 145 231 77 59 61 53
Nuclear Periphery 3 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 3 3 1 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 1 0 19 11 6 6 8 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2
SpindlePole 3 4 6 6 11 6 8 12 6 11 0 14 2 1 1 6 3 10 26 26 17 15 37 30
Vac/Vac Membrane 28 31 11 0 13 18 14 13 3 5 2 3 32 64 60 52 42 41 50 114 68 112 176 161
Unique Cell Count 226 369 119 84 164 154 223 201 246 197 153 160 267 238 276 317 246 210 300 494 235 304 506 508
Labelled Cell Count 256 432 132 89 184 180 270 248 322 277 198 218 285 252 290 366 279 245 300 494 235 304 506 508


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 3.8 4.8 4.6 5.0 3.9 4.3 4.5 3.5 3.5 3.2 3.7 4.9 5.2 5.0 6.4 6.6 6.3 4.6 4.6 5.1
Std Deviation (1e-4) 1.2 0.6 0.9 1.4 1.0 0.9 1.8 1.7 1.3 1.1 1.0 0.8 1.2 1.7 1.7 1.6 1.8 1.6 1.5 1.6 2.2
Intensity Change (Log2) – – – -0.06 0.06 -0.31 -0.14 -0.09 -0.44 -0.46 -0.58 -0.35 0.03 0.12 0.08 0.43 0.48 0.4 -0.04 -0.04 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.9 2.0 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5934 2.2907 1.8079 1.6655 1.0473 1.8265 0.5992 0.4963 0.3538 0.6031 0.0247 0.2948 -0.0689 0.0729 0.0568 0.4721 0.5369 0.2629
Actin 0.0383 0.0002 0.0003 0.0015 0.0006 0.0024 0.0137 0.0002 0.0017 0.0014 0.0039 0.0015 0.0125 0.0002 0.0001 0.0113 0.0052 0.0035
Bud 0.0004 0.0001 0.0001 0.0005 0 0.0001 0.0034 0.0061 0.0003 0.0002 0.0005 0.0002 0.0004 0.0015 0.0001 0.0002 0.0002 0.0003
Bud Neck 0.0163 0.0009 0.0015 0.0053 0.0024 0.0088 0.0039 0.0006 0.0077 0.0019 0.002 0.0039 0.0039 0.0008 0.0005 0.0034 0.0038 0.004
Bud Periphery 0.0008 0.0001 0.0001 0.002 0.0001 0.0003 0.002 0.0006 0.0007 0.0005 0.0008 0.0002 0.0003 0.0007 0.0001 0.0002 0.0003 0.0004
Bud Site 0.0095 0.0024 0.0006 0.0045 0.0002 0.0004 0.0057 0.0029 0.0037 0.0004 0.0028 0.0002 0.0022 0.0039 0.0003 0.0003 0.0003 0.0002
Cell Periphery 0.0005 0.0001 0.0001 0.0021 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0 0.0001 0 0 0.0001 0.0001 0
Cytoplasm 0.0302 0.0504 0.021 0.0329 0.0557 0.0305 0.0228 0.0353 0.0159 0.0108 0.0346 0.0117 0.0281 0.0348 0.0232 0.0149 0.0179 0.0146
Cytoplasmic Foci 0.0156 0.0017 0.0033 0.0057 0.0021 0.0041 0.0185 0.0009 0.0062 0.0167 0.0187 0.0019 0.0108 0.0022 0.0025 0.0104 0.0121 0.011
Eisosomes 0.0007 0.0001 0.0002 0.0006 0.0002 0.0002 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0001 0.0002 0
Endoplasmic Reticulum 0.0026 0.0025 0.0018 0.0086 0.0075 0.0017 0.0041 0.0018 0.0021 0.0012 0.005 0.0007 0.0021 0.0012 0.0007 0.0024 0.0023 0.001
Endosome 0.0094 0.0017 0.0051 0.0169 0.0066 0.0036 0.0353 0.0019 0.0145 0.0413 0.0339 0.0017 0.0086 0.001 0.0009 0.0047 0.0038 0.0029
Golgi 0.0056 0.0001 0.0008 0.0026 0.0002 0.0004 0.0103 0 0.0047 0.0032 0.0096 0.0004 0.0014 0 0 0.001 0.0005 0.0006
Lipid Particles 0.0133 0.0003 0.0058 0.0058 0.0015 0.0021 0.0201 0.0001 0.0059 0.0082 0.0161 0.0017 0.0026 0.0001 0.0001 0.001 0.0009 0.0015
Mitochondria 0.0038 0.0008 0.001 0.0299 0.033 0.0012 0.007 0.0006 0.0026 0.0027 0.0049 0.0005 0.0009 0.0006 0.0007 0.001 0.0021 0.002
None 0.1134 0.21 0.1401 0.1342 0.1572 0.0968 0.0205 0.0217 0.0145 0.0094 0.0589 0.023 0.0446 0.1209 0.0474 0.0588 0.0592 0.0295
Nuclear Periphery 0.0137 0.0292 0.025 0.0212 0.0368 0.014 0.0361 0.0189 0.0168 0.0211 0.0308 0.0112 0.0163 0.0141 0.0186 0.0322 0.0433 0.0124
Nucleolus 0.0242 0.018 0.0379 0.0845 0.034 0.0356 0.0147 0.0153 0.0178 0.0189 0.0177 0.0255 0.0089 0.0097 0.0124 0.0076 0.0125 0.0205
Nucleus 0.6175 0.6589 0.6694 0.5396 0.5917 0.7142 0.7183 0.8456 0.8273 0.7706 0.6954 0.8619 0.6969 0.6404 0.6777 0.6832 0.6032 0.6602
Peroxisomes 0.01 0.0001 0.0018 0.0015 0.0007 0.0004 0.0058 0 0.0029 0.0038 0.0114 0.0015 0.0018 0.0001 0 0.0003 0.0003 0.0004
Punctate Nuclear 0.0712 0.0197 0.0812 0.085 0.0656 0.0814 0.0479 0.0448 0.0506 0.0758 0.049 0.0512 0.1559 0.1668 0.2139 0.1658 0.2292 0.2338
Vacuole 0.0023 0.0023 0.0022 0.0136 0.0034 0.0016 0.0061 0.0021 0.0029 0.0075 0.0026 0.001 0.0013 0.0008 0.0004 0.0006 0.0016 0.0006
Vacuole Periphery 0.0006 0.0004 0.0007 0.0015 0.0005 0.0004 0.0031 0.0004 0.0009 0.0043 0.0013 0.0003 0.0005 0.0002 0.0003 0.0005 0.001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.0783 11.871 6.4064 10.3759 14.087 6.8372 9.9114 12.1516 11.9172 13.9489
Translational Efficiency 0.8425 0.8669 1.1142 0.6826 0.6026 1.4253 0.7324 0.609 0.5852 0.66

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Sterol regulatory element binding protein; induces sterol biosynthetic genes, upon sterol depletion; acts as a sterol sensor, binding ergosterol in sterol rich conditions; relocates from intracellular membranes to perinuclear foci upon sterol depletion; redundant activator of filamentation with ECM22, up-regulating the expression of filamentous growth genes; contains a Zn[2]-Cys[6] binuclear cluster; UPC2 has a paralog, ECM22, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Upc2

Upc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Upc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available