Standard name
Human Ortholog
Description DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.07 0.12 0.13 0.14 0.15 0.16 0.12 0.11 0.12 0.12 0.19 0.24 0.11 0.13 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.66 0.8 0.52 0.75 0.75 0.77 0.78 0.85 0.82 0.79 0 0 0.08 0 0 0.07 0 0 0 0 0 0
Nucleus 0.94 0.87 0.72 0.74 0.62 0.49 0.48 0.41 0.37 0.33 0.4 0.34 0.82 0.77 0.69 0.79 0.74 0.75 0.81 0.82 0.69 0.73 0.65 0.52
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.08 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.08 0.06 0.07 0.18 0.1 0.14 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Bud 0 0 4 1 3 5 9 17 2 4 9 12 0 0 0 1 0 0 4 6 0 0 6 8
Bud Neck 0 0 3 0 1 1 1 7 0 1 2 0 0 0 0 0 0 0 0 1 1 0 1 1
Bud Site 0 0 0 2 8 10 20 20 9 14 15 18 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 11 15 19 12 33 34 69 73 46 40 36 47 17 34 48 54 40 41 3 3 4 0 1 8
Endoplasmic Reticulum 1 3 0 0 0 0 0 0 0 0 0 0 0 1 1 13 15 12 0 1 0 1 2 1
Endosome 3 3 0 0 1 0 0 0 0 0 0 0 1 2 2 17 13 6 5 7 1 1 6 8
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 2 3 0 0 0 4
Mitochondria 3 29 190 137 148 192 379 370 219 279 264 311 5 3 16 18 4 22 4 7 7 0 1 6
Nucleus 401 398 207 127 177 127 243 200 103 110 130 135 119 140 141 379 235 234 383 409 214 67 162 141
Nuclear Periphery 1 3 0 2 0 0 1 0 1 2 0 0 0 0 1 1 1 1 1 1 0 0 0 0
Nucleolus 2 4 0 0 1 1 1 4 1 0 1 0 2 2 1 3 1 0 23 12 8 6 20 15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 3 7 2 11 4 19 11 7 7 7 13 1 6 2 9 9 6 8 7 6 1 6 13
Vac/Vac Membrane 15 23 0 0 2 1 2 2 1 4 3 9 3 2 6 38 28 26 29 32 55 9 35 57
Unique Cell Count 428 456 289 171 285 257 503 483 281 330 322 392 145 182 204 480 318 310 472 499 313 92 251 275
Labelled Cell Count 440 483 431 283 385 375 744 704 389 462 467 545 148 190 219 537 347 348 472 499 313 92 251 275


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 5.2 3.3 3.3 3.6 3.3 3.0 3.0 2.7 2.6 2.7 2.7 5.4 5.4 4.7 7.4 7.5 7.3 5.3 5.7 6.1
Std Deviation (1e-4) 0.7 1.0 0.4 0.7 1.3 1.4 1.0 1.0 1.3 1.1 1.1 1.2 1.3 1.5 1.4 2.2 1.7 1.6 1.8 1.7 2.1
Intensity Change (Log2) – – – -0.01 0.12 -0.02 -0.13 -0.15 -0.27 -0.33 -0.29 -0.28 0.71 0.71 0.51 1.16 1.18 1.14 0.68 0.79 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 2.1 2.6 3.1 3.5 3.7 2.3 2.0 2.4 1.8 4.0 5.4 2.1 2.5 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.7 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 3.2 3.5 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -12.3 -13.8 0 -18.7 -17.0 -15.0
Nucleus 0.6 -2.4 -5.3 -6.4 -8.1 -8.4 -9.5 -7.8 -9.6 2.4 1.3 -0.6 2.3 0.6 1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.6 0 -0.5 0.3 -0.4
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 4.9 5.2 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0187 1.8034 1.4883 1.2437 1.2946 1.4753 1.6282 2.7194 2.0068 2.1793 1.7845 2.1974 3.8364 4.2215 3.9643 4.1842 3.197 4.2882
Actin 0 0.0001 0 0.0001 0.0002 0 0.0226 0.0011 0.0032 0.0006 0.0046 0.0011 0.0089 0 0 0.0012 0.0004 0.0001
Bud 0 0.0001 0 0.0001 0.0001 0.0001 0.0008 0.0001 0.0001 0.0004 0.0001 0.0001 0.0005 0.0002 0 0.0006 0.0001 0.0005
Bud Neck 0.0004 0.0005 0.0003 0.0012 0.0014 0.0051 0.0012 0.0003 0.002 0.0066 0.0008 0.0014 0.0009 0.0002 0.0002 0.0114 0.008 0.0018
Bud Periphery 0 0 0 0.0001 0.0002 0.0001 0.0029 0.0001 0.0001 0.001 0.0001 0.0004 0.0004 0 0 0.0011 0.0004 0.0001
Bud Site 0.0002 0.0007 0.0001 0.0008 0.0005 0.0002 0.0026 0.0002 0.0014 0.005 0.0004 0.0004 0.003 0.0023 0.0002 0.0119 0.0006 0.0001
Cell Periphery 0 0.0001 0 0.0001 0.0001 0 0.0004 0 0.0001 0.0005 0.0001 0 0.0001 0 0 0.0004 0.0001 0
Cytoplasm 0.0023 0.008 0.002 0.0007 0.015 0.0036 0.0087 0.0126 0.0118 0.0085 0.0066 0.0055 0.0038 0.0034 0.0044 0.0019 0.0046 0.007
Cytoplasmic Foci 0.0001 0.0003 0 0.0012 0.0037 0.0004 0.0109 0.0023 0.0085 0.0029 0.0032 0.0004 0.0011 0.0043 0.0001 0.0001 0.0003 0.0007
Eisosomes 0 0 0 0 0 0 0.0006 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0001 0.0007 0.0001 0.0001 0.0011 0.0001 0.0031 0.0002 0.0009 0.0004 0.0028 0.0002 0.0004 0.0001 0.0001 0.0002 0.0002 0.0009
Endosome 0.0001 0.0011 0.0001 0.0013 0.0067 0.0004 0.0174 0.0005 0.007 0.0087 0.0186 0.0009 0.0015 0.0001 0.0001 0.0002 0.0006 0.0013
Golgi 0 0 0 0.0003 0.0002 0 0.0055 0.0002 0.0014 0.0027 0.0041 0.0004 0.0011 0 0 0.0001 0.0001 0
Lipid Particles 0 0.0001 0 0.0017 0.0018 0.0001 0.0311 0.0017 0.0079 0.0128 0.0047 0.0007 0.0016 0.0001 0 0.0001 0.0003 0.0002
Mitochondria 0.0002 0.0003 0.0001 0.0004 0.0003 0.0001 0.0067 0.0003 0.0007 0.0048 0.0022 0.0021 0.0008 0.0002 0.0001 0.0007 0.0014 0.0001
None 0.016 0.0111 0.0121 0.0004 0.0099 0.001 0.0698 0.0576 0.0887 0.1072 0.1319 0.0937 0.0031 0.0191 0.011 0.0011 0.0048 0.018
Nuclear Periphery 0.0026 0.0089 0.0064 0.0023 0.0123 0.0017 0.0154 0.0034 0.0056 0.0047 0.0056 0.0036 0.003 0.0021 0.0023 0.0039 0.0047 0.0074
Nucleolus 0.0082 0.0124 0.0108 0.0273 0.0288 0.029 0.0167 0.012 0.01 0.0329 0.0333 0.0221 0.0143 0.0168 0.0139 0.0208 0.0782 0.0269
Nucleus 0.9609 0.946 0.9505 0.9042 0.8327 0.9352 0.7132 0.8743 0.8107 0.7724 0.6973 0.8148 0.9391 0.9154 0.9319 0.9282 0.8344 0.894
Peroxisomes 0 0 0 0.0003 0.0001 0 0.0096 0.0051 0.0063 0.002 0.0012 0.0005 0.0009 0 0 0.0002 0.0002 0
Punctate Nuclear 0.0085 0.0079 0.0172 0.0568 0.0829 0.0224 0.0559 0.0276 0.0317 0.0201 0.0813 0.0509 0.0148 0.0351 0.0355 0.0157 0.0598 0.0403
Vacuole 0.0002 0.0017 0.0001 0.0005 0.0019 0.0004 0.0035 0.0002 0.0015 0.005 0.0006 0.0002 0.0005 0.0004 0.0001 0.0003 0.0007 0.0004
Vacuole Periphery 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0016 0.0001 0.0004 0.0009 0.0004 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.5998 8.0316 9.6846 14.8192 15.289 15.9947 13.323 14.2162 14.4838 18.9272
Translational Efficiency 1.243 1.3915 1.0691 0.6794 0.8144 1.1384 0.6999 0.5754 0.6425 0.7139

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rad9

Rad9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available