Standard name
Human Ortholog
Description Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminally propionylated in vivo

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.99 0.97 0.97 0.92 0.88 0.79 0.79 0.68 0.78 1.0 0.99 0.99 0.87 0.87 0.77 0.87 0.85 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.13 0 0.2 0.21 0.42 0.37 0.51 0.41 0 0 0 0 0 0.07 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 4 0 2 0 2 5
Bud 1 0 0 1 1 6 15 8 13 9 11 0 0 1 5 2 1 0 5 13
Bud Neck 3 1 1 0 3 4 22 2 7 3 3 0 0 0 2 0 0 0 3 4
Bud Site 0 0 0 0 0 0 0 0 1 1 7 1 0 0
Cell Periphery 3 0 0 3 0 0 0 2 1 2 3 1 0 1 2 0 1 2 2 3
Cytoplasm 595 148 178 226 271 365 570 308 343 255 422 532 661 744 508 134 108 167 383 432
Endoplasmic Reticulum 2 1 0 0 1 0 0 1 0 0 0 0 1 2 1 0 0 1 1 4
Endosome 0 0 0 0 0 1 1 0 0 0 0 0 0 1 3 1 1 1 3 7
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 3 1
Mitochondria 12 4 3 31 9 79 136 164 158 190 225 2 2 0 22 6 10 8 20 21
Nucleus 1 0 0 0 0 0 2 1 0 0 3 0 0 1 0 0 0 0 0 1
Nuclear Periphery 1 0 0 0 0 0 1 0 0 0 1 0 1 1 0 0 0 0 0 1
Nucleolus 0 0 0 2 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 1 0 0 1 5 3 24 4 6 5 14 1 2 0 6 0 1 1 4 13
Vac/Vac Membrane 0 0 0 0 1 4 1 1 0 1 2 0 0 0 7 3 2 2 10 16
Unique Cell Count 610 152 179 232 279 398 649 392 432 373 543 534 665 748 582 154 140 193 449 545
Labelled Cell Count 620 155 182 264 291 462 773 492 530 467 692 537 667 751 582 154 140 193 449 545


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.0 2.8 2.9 3.5 2.8 2.9 2.2 2.6 2.5 2.4 4.0 4.0 4.0 4.6 4.4 4.6 3.6 4.0 4.2
Std Deviation (1e-4) 0.6 1.0 0.7 1.7 1.8 1.3 1.3 0.8 1.0 0.9 0.9 1.4 1.1 1.3 1.3 1.1 1.7 0.6 1.2 1.8
Intensity Change (Log2) 0.05 0.32 -0.0 0.06 -0.31 -0.11 -0.16 -0.2 0.53 0.55 0.53 0.72 0.66 0.74 0.38 0.51 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5464 -0.2754 -0.6241 -0.3826 -0.3272 -0.5999 -1.3787 -1.1759 -1.348 -1.6426 -1.495 -1.2122 -0.0987 0.1159 0.2791 0.4529 0.6906 0.177
Actin 0.0348 0.0151 0.026 0.0195 0.0324 0.01 0.0259 0.0003 0.0164 0.0003 0.0007 0.0021 0.0138 0.0031 0.0091 0.034 0.011 0.0324
Bud 0.0006 0.004 0.0004 0.0011 0.0003 0.0004 0.0004 0.0041 0.0008 0.0001 0.0002 0.0001 0.0004 0.0044 0.0009 0.0015 0.0133 0.0017
Bud Neck 0.0019 0.0004 0.0006 0.0005 0.003 0.0007 0.0027 0.0001 0.0018 0 0.0004 0.0002 0.0133 0.0002 0.0008 0.0045 0.0024 0.0013
Bud Periphery 0.0008 0.0035 0.0007 0.0056 0.0004 0.0008 0.0008 0.0007 0.0012 0.0005 0.0004 0.0003 0.0008 0.006 0.0019 0.0063 0.0148 0.0078
Bud Site 0.0039 0.0064 0.0077 0.0028 0.0014 0.0001 0.0043 0.0088 0.0127 0 0.0053 0.0001 0.0096 0.0111 0.0138 0.0017 0.0164 0.0006
Cell Periphery 0.0003 0.0002 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0003 0 0.0001 0.0001 0.0004 0.0002 0.0002 0.0007 0.0007 0.0004
Cytoplasm 0.0308 0.0178 0.0212 0.0095 0.0365 0.057 0.0297 0.0363 0.0286 0.0098 0.0151 0.0483 0.0141 0.0295 0.03 0.0393 0.0107 0.043
Cytoplasmic Foci 0.031 0.0092 0.0174 0.0069 0.0232 0.0089 0.0284 0.006 0.0159 0.0063 0.0074 0.0073 0.0076 0.0011 0.0121 0.0187 0.033 0.005
Eisosomes 0.0006 0.0002 0.0004 0.0008 0.0003 0.0002 0.0006 0.0003 0.0004 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0.0002 0.0002 0.0004
Endoplasmic Reticulum 0.0032 0.0005 0.0016 0.0015 0.006 0.0007 0.0024 0.0004 0.0011 0.0001 0.0006 0.0004 0.0006 0.0004 0.001 0.0014 0.0104 0.0009
Endosome 0.0086 0.0003 0.003 0.0167 0.0487 0.0019 0.0176 0.0003 0.0051 0.0002 0.0048 0.0043 0.0104 0.0005 0.0117 0.0153 0.0231 0.0044
Golgi 0.0037 0.0006 0.0015 0.0053 0.0171 0.0018 0.0062 0.0001 0.0038 0.0001 0.0016 0.0037 0.0056 0.0003 0.0025 0.0073 0.0114 0.0022
Lipid Particles 0.0092 0.0008 0.0021 0.0089 0.0351 0.0049 0.0151 0.0002 0.0032 0.0006 0.0072 0.0022 0.005 0.0001 0.0043 0.0106 0.0464 0.0018
Mitochondria 0.0112 0.0009 0.0011 0.0225 0.0106 0.0013 0.0037 0.0007 0.0121 0.0018 0.002 0.0059 0.0027 0.0014 0.0016 0.0027 0.003 0.0022
None 0.8187 0.9308 0.8979 0.8692 0.7086 0.89 0.8255 0.936 0.8825 0.9736 0.9344 0.9129 0.8961 0.9405 0.899 0.8251 0.7179 0.8867
Nuclear Periphery 0.009 0.0006 0.0015 0.0063 0.0256 0.0023 0.0052 0.0002 0.0005 0.0002 0.0024 0.0037 0.0008 0.0002 0.001 0.0009 0.007 0.0026
Nucleolus 0.001 0.001 0.0003 0.0015 0.0008 0.0004 0.0007 0.001 0.0004 0.0003 0.0009 0.0003 0.0014 0.0002 0.0004 0.0006 0.0014 0.0004
Nucleus 0.0057 0.0023 0.0008 0.0051 0.0047 0.0063 0.0015 0.0014 0.0004 0.0003 0.0012 0.0027 0.0043 0.0003 0.0008 0.001 0.0073 0.0013
Peroxisomes 0.0115 0.0008 0.0096 0.0045 0.0313 0.0096 0.0207 0.0003 0.0083 0.0036 0.0122 0.0023 0.0071 0.0001 0.0022 0.0232 0.0549 0.0018
Punctate Nuclear 0.012 0.0039 0.0054 0.009 0.0068 0.002 0.0041 0.0023 0.0033 0.0018 0.002 0.0023 0.002 0.0002 0.004 0.001 0.0055 0.0022
Vacuole 0.0011 0.0006 0.0003 0.0018 0.0028 0.0003 0.0033 0.0003 0.0008 0.0001 0.0007 0.0006 0.0031 0.0002 0.0019 0.0033 0.0073 0.0006
Vacuole Periphery 0.0006 0.0001 0.0001 0.0009 0.0043 0.0003 0.0009 0.0001 0.0004 0 0.0003 0.0003 0.0007 0.0001 0.0007 0.0006 0.0019 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1433.8727 1048.1792 139.4159 99.628 1077.4409 762.8347 558.0265 304.5096 250.5358 881.7015
Translational Efficiency 2.2165 2.0079 1.6007 2.6634 2.3982 3.2635 2.2362 2.2948 1.7935 2.3991

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminally propionylated in vivo
Localization
Cell Percentages cytoplasm (22%), nucleus (8%), mixed (31%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Hta1

Hta1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hta1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available