Standard name | |
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Human Ortholog | |
Description | Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress; mutations affecting Adk1p catalytic activity deregulate expression of phosphate utilization genes PHO5 and PHO84; human homolog AK1 can complement yeast adk1 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 4 | 1 | 0 | 0 | 1 | 1 | 4 | 0 | 4 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 3 | 4 | 1 | 5 | 8 | 8 | 16 | 13 | 24 | 9 | 10 | 2 | 2 | 3 | 5 | 8 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 360 | 304 | 526 | 380 | 393 | 285 | 371 | 542 | 354 | 502 | 225 | 212 | 356 | 539 | 571 | 546 | 447 | 508 | 333 | 306 | 514 | 291 | 468 | 515 |
Endoplasmic Reticulum | 1 | 3 | 1 | 5 | 11 | 14 | 28 | 57 | 19 | 50 | 9 | 23 | 4 | 3 | 3 | 17 | 17 | 25 | 1 | 0 | 2 | 0 | 5 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 0 | 5 | 3 | 2 | 3 | 2 | 1 | 2 | 1 | 1 | 1 | 5 | 4 | 3 | 1 | 2 | 1 | 3 | 5 | 6 |
Unique Cell Count | 362 | 307 | 530 | 385 | 406 | 312 | 411 | 616 | 386 | 569 | 244 | 242 | 360 | 543 | 578 | 554 | 453 | 512 | 346 | 318 | 533 | 306 | 492 | 539 |
Labelled Cell Count | 364 | 310 | 533 | 386 | 409 | 316 | 413 | 621 | 395 | 581 | 250 | 252 | 363 | 545 | 583 | 574 | 478 | 538 | 346 | 318 | 533 | 306 | 492 | 539 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 121.1 | 118.8 | 124.7 | 113.0 | 101.9 | 65.2 | 75.9 | 73.1 | 68.6 | 67.2 | 62.3 | 63.2 | 133.9 | 130.4 | 133.0 | 152.4 | 163.4 | 144.9 | 106.6 | 115.3 | 122.8 |
Std Deviation (1e-4) | 30.0 | 29.4 | 25.5 | 24.2 | 22.5 | 23.4 | 16.2 | 17.4 | 16.9 | 16.4 | 16.7 | 16.0 | 42.4 | 35.6 | 30.7 | 48.5 | 52.8 | 45.9 | 29.9 | 29.8 | 30.4 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.29 | -0.94 | -0.72 | -0.77 | -0.86 | -0.89 | -1.0 | -0.98 | 0.1 | 0.06 | 0.09 | 0.29 | 0.39 | 0.22 | -0.23 | -0.11 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 1.6 | 2.4 | 2.9 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 |
Cytoplasm | -0.8 | -2.8 | -5.9 | -6.5 | -7.5 | -5.8 | -7.4 | -5.4 | -7.3 | -0.6 | 0 | -0.8 | -1.1 | -0.9 | 0 |
Endoplasmic Reticulum | 0 | 3.4 | 4.6 | 5.8 | 7.0 | 4.8 | 6.8 | 0 | 6.9 | 0 | 0 | 0 | 3.7 | 4.2 | 4.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 71.0849 | 103.7059 | 90.827 | 98.5705 | 89.995 | 98.5482 | 106.7867 | 119.3117 | 115.4978 | 107.6903 | 107.298 | 114.8243 | 122.2732 | 135.0805 | 137.6322 | 122.8823 | 125.5775 | 123.2696 |
Actin | 0.0199 | 0.0006 | 0.0129 | 0 | 0.0001 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0002 |
Bud | 0.0027 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 |
Bud Neck | 0.0041 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 |
Bud Periphery | 0.0019 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0085 | 0.0047 | 0.0011 | 0 | 0.0004 | 0 | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0011 | 0.0001 | 0.0006 | 0 | 0 | 0 | 0.0015 | 0.0022 | 0 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8125 | 0.9843 | 0.8842 | 0.99 | 0.9222 | 0.9846 | 0.9332 | 0.9867 | 0.991 | 0.9792 | 0.9885 | 0.9583 | 0.9545 | 0.9944 | 0.9942 | 0.9919 | 0.9901 | 0.9879 |
Cytoplasmic Foci | 0.0169 | 0.0001 | 0.0266 | 0.0001 | 0.0058 | 0.0001 | 0.0021 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0023 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 |
Eisosomes | 0.0004 | 0 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0024 | 0.0004 | 0.0014 | 0.0002 | 0.0002 | 0.0002 | 0.0017 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Endosome | 0.0101 | 0.0002 | 0.0039 | 0 | 0.0494 | 0 | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Golgi | 0.0025 | 0 | 0.003 | 0 | 0.0061 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0066 | 0.0001 | 0.0036 | 0 | 0.0039 | 0 | 0.0016 | 0.0005 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0107 | 0.0002 | 0.0056 | 0 | 0.0012 | 0.0001 | 0.0012 | 0.0001 | 0 | 0 | 0 | 0 | 0.002 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 |
None | 0.0168 | 0.0002 | 0.005 | 0.0001 | 0.0001 | 0.0003 | 0.0051 | 0.0017 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0012 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.0095 | 0.0031 | 0.0033 | 0.0013 | 0.0008 | 0.0015 | 0.0078 | 0.0015 | 0.0011 | 0.0027 | 0.0015 | 0.0035 | 0.0028 | 0.0006 | 0.0007 | 0.0006 | 0.0011 | 0.0011 |
Nucleolus | 0.0199 | 0.0001 | 0.0165 | 0 | 0.0001 | 0 | 0.0074 | 0.0001 | 0 | 0 | 0 | 0 | 0.0076 | 0.0001 | 0 | 0 | 0 | 0 |
Nucleus | 0.0295 | 0.0052 | 0.0147 | 0.0079 | 0.005 | 0.0118 | 0.0269 | 0.0046 | 0.007 | 0.0161 | 0.0083 | 0.0369 | 0.0242 | 0.0028 | 0.0045 | 0.007 | 0.0079 | 0.0091 |
Peroxisomes | 0.0045 | 0 | 0.0012 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0109 | 0.0003 | 0.0058 | 0 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0027 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Vacuole | 0.0067 | 0.0002 | 0.0055 | 0.0002 | 0.0026 | 0.0003 | 0.0052 | 0.001 | 0.0002 | 0.0004 | 0.0007 | 0.0003 | 0.0008 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0003 |
Vacuole Periphery | 0.0017 | 0.0001 | 0.0037 | 0.0001 | 0.0006 | 0.0001 | 0.0012 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 624.2729 | 628.096 | 595.1748 | 571.4488 | 553.8543 | 728.602 | 680.5357 | 682.685 | 704.8339 | 620.2966 |
Translational Efficiency | 1.948 | 1.9738 | 1.9665 | 2.0143 | 2.2741 | 2.1616 | 2.004 | 2.103 | 2.1785 | 2.182 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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