Standard name | |
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Human Ortholog | |
Description | SIR protein involved in assembly of silent chromatin domains; silent information regulator (SIR) along with SIR2 and SIR3; involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; some alleles of SIR4 prolong lifespan; required for telomere hypercluster formation in quiescent yeast cells |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 1 | 2 | 4 |
Bud | 0 | 3 | 1 | 5 | 9 | 5 | 6 | 16 | 8 | – | 7 | 10 | 1 | 0 | 3 | 2 | 0 | 0 | 5 | 3 | 1 | 2 | 2 | 4 |
Bud Neck | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 2 | 1 | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 2 | 3 | 2 | 1 | 3 |
Bud Site | 0 | 0 | 3 | 5 | 1 | 1 | 10 | 10 | 3 | – | 5 | 14 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 20 | 31 | 20 | 23 | 32 | 25 | 37 | 30 | 11 | – | 14 | 23 | 44 | 62 | 69 | 46 | 24 | 19 | 5 | 9 | 2 | 6 | 7 | 6 |
Endoplasmic Reticulum | 0 | 3 | 2 | 2 | 2 | 2 | 1 | 2 | 0 | – | 1 | 0 | 0 | 2 | 1 | 7 | 6 | 1 | 0 | 2 | 2 | 0 | 1 | 3 |
Endosome | 152 | 119 | 20 | 15 | 74 | 10 | 8 | 2 | 2 | – | 3 | 4 | 47 | 70 | 45 | 41 | 32 | 35 | 196 | 152 | 104 | 67 | 124 | 111 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 3 | 2 | 1 | 2 | 0 |
Mitochondria | 7 | 109 | 114 | 188 | 109 | 145 | 253 | 294 | 196 | – | 200 | 255 | 23 | 21 | 62 | 21 | 4 | 15 | 18 | 45 | 39 | 17 | 19 | 24 |
Nucleus | 41 | 68 | 24 | 19 | 12 | 19 | 22 | 34 | 16 | – | 16 | 20 | 23 | 24 | 23 | 27 | 19 | 13 | 22 | 62 | 17 | 20 | 7 | 14 |
Nuclear Periphery | 25 | 13 | 8 | 7 | 5 | 4 | 3 | 5 | 7 | – | 12 | 6 | 8 | 2 | 2 | 15 | 8 | 7 | 65 | 69 | 34 | 12 | 25 | 34 |
Nucleolus | 25 | 9 | 4 | 4 | 5 | 5 | 4 | 12 | 3 | – | 12 | 11 | 9 | 4 | 7 | 14 | 14 | 15 | 31 | 39 | 23 | 16 | 10 | 15 |
Peroxisomes | 1 | 2 | 1 | 0 | 15 | 0 | 0 | 0 | 0 | – | 0 | 1 | 2 | 5 | 2 | 0 | 0 | 2 | 2 | 2 | 6 | 2 | 6 | 5 |
SpindlePole | 77 | 32 | 50 | 29 | 41 | 9 | 25 | 33 | 4 | – | 9 | 19 | 19 | 22 | 23 | 19 | 14 | 10 | 99 | 98 | 69 | 61 | 91 | 98 |
Vac/Vac Membrane | 56 | 112 | 10 | 18 | 28 | 5 | 12 | 5 | 9 | – | 7 | 8 | 27 | 27 | 28 | 29 | 17 | 16 | 11 | 29 | 16 | 17 | 10 | 17 |
Unique Cell Count | 304 | 397 | 222 | 250 | 254 | 196 | 309 | 349 | 213 | 226 | 288 | 167 | 199 | 225 | 169 | 102 | 98 | 484 | 548 | 346 | 242 | 331 | 365 | |
Labelled Cell Count | 405 | 502 | 259 | 315 | 334 | 230 | 382 | 445 | 260 | 286 | 371 | 203 | 241 | 265 | 226 | 139 | 135 | 484 | 548 | 346 | 242 | 331 | 365 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 4.4 | 3.7 | 3.5 | 3.9 | 3.5 | 3.3 | 3.2 | 2.8 | – | 3.0 | 2.8 | 4.5 | 4.7 | 4.3 | 6.8 | 7.4 | 6.9 | 4.7 | 4.9 | 5.2 |
Std Deviation (1e-4) | 0.7 | 1.0 | 0.9 | 1.3 | 1.4 | 1.4 | 1.3 | 0.9 | 1.0 | – | 1.1 | 0.9 | 1.1 | 1.5 | 1.5 | 2.4 | 2.5 | 2.3 | 1.3 | 1.8 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.06 | -0.1 | -0.18 | -0.21 | -0.42 | – | -0.29 | -0.42 | 0.28 | 0.33 | 0.2 | 0.87 | 1.0 | 0.88 | 0.34 | 0.39 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.1 | 3.7 | 3.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4047 | 3.0021 | 2.6803 | 2.7947 | 2.4848 | 3.1309 | -1.1993 | -1.0745 | -1.0847 | -1.0415 | -1.2074 | -0.992 | 3.25 | 3.62 | 3.6033 | 4.7283 | 3.7564 | 3.622 |
Actin | 0.0044 | 0.002 | 0.0061 | 0.0035 | 0.0039 | 0.0037 | 0.0005 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0005 | 0.0676 | 0.0029 | 0.0116 | 0.0669 | 0.0022 | 0.0023 |
Bud | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0.001 | 0.0001 | 0.0003 | 0.0021 | 0 | 0.0001 |
Bud Neck | 0.0008 | 0.0021 | 0.0015 | 0.0011 | 0.0106 | 0.0082 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0012 | 0.0008 | 0.0004 | 0.0004 | 0.0009 | 0.0006 | 0.0072 |
Bud Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0027 | 0.0001 | 0.0008 | 0.0056 | 0 | 0.0003 |
Bud Site | 0.0004 | 0.0024 | 0.0019 | 0.0004 | 0.0013 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0007 | 0.0015 | 0.001 | 0.0042 | 0.0001 | 0.0005 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 |
Cytoplasm | 0.0102 | 0.0286 | 0.0188 | 0.0309 | 0.0411 | 0.0401 | 0.0036 | 0.0035 | 0.0011 | 0.0013 | 0.0021 | 0.0009 | 0.0054 | 0.0152 | 0.0067 | 0.0062 | 0.0112 | 0.0104 |
Cytoplasmic Foci | 0.0158 | 0.0302 | 0.0333 | 0.0278 | 0.0236 | 0.0109 | 0.0028 | 0.003 | 0.0019 | 0.002 | 0.004 | 0.0006 | 0.0116 | 0.0211 | 0.015 | 0.012 | 0.0132 | 0.0093 |
Eisosomes | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0003 | 0.0004 | 0.0008 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0037 | 0.0101 | 0.0039 | 0.0083 | 0.0072 | 0.0076 | 0.0009 | 0.0013 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0043 | 0.013 | 0.0039 | 0.0034 | 0.0064 | 0.0025 |
Endosome | 0.0229 | 0.0303 | 0.0192 | 0.02 | 0.0308 | 0.0152 | 0.0054 | 0.0038 | 0.0017 | 0.004 | 0.0025 | 0.0012 | 0.0212 | 0.0313 | 0.0194 | 0.0336 | 0.008 | 0.0086 |
Golgi | 0.003 | 0.003 | 0.0047 | 0.0026 | 0.0043 | 0.0017 | 0.0009 | 0.0005 | 0.0004 | 0.0004 | 0.0007 | 0.0004 | 0.0102 | 0.0047 | 0.0115 | 0.0183 | 0.0011 | 0.0018 |
Lipid Particles | 0.0376 | 0.0389 | 0.0635 | 0.0351 | 0.0455 | 0.0115 | 0.0129 | 0.008 | 0.0061 | 0.0038 | 0.0265 | 0.0046 | 0.042 | 0.0922 | 0.0867 | 0.0489 | 0.0099 | 0.0304 |
Mitochondria | 0.0013 | 0.0008 | 0.001 | 0.0005 | 0.0011 | 0.0019 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0042 | 0.0008 | 0.0309 | 0.0763 | 0.0003 | 0.0005 |
None | 0.0042 | 0.0339 | 0.017 | 0.0335 | 0.0095 | 0.0216 | 0.002 | 0.0044 | 0.0007 | 0.0009 | 0.0011 | 0.0004 | 0.007 | 0.0717 | 0.0626 | 0.0067 | 0.1965 | 0.0133 |
Nuclear Periphery | 0.3395 | 0.2379 | 0.2336 | 0.3215 | 0.3818 | 0.2549 | 0.5158 | 0.4173 | 0.4524 | 0.3892 | 0.4351 | 0.3963 | 0.574 | 0.3099 | 0.5067 | 0.1088 | 0.3816 | 0.3552 |
Nucleolus | 0.0102 | 0.024 | 0.014 | 0.004 | 0.0609 | 0.0289 | 0.0157 | 0.0278 | 0.0207 | 0.0098 | 0.0502 | 0.0454 | 0.008 | 0.0056 | 0.0057 | 0.016 | 0.0075 | 0.0556 |
Nucleus | 0.1042 | 0.1465 | 0.1101 | 0.103 | 0.0838 | 0.1662 | 0.1125 | 0.1767 | 0.1521 | 0.1519 | 0.1622 | 0.1819 | 0.019 | 0.0534 | 0.0292 | 0.3009 | 0.0282 | 0.1613 |
Peroxisomes | 0.0024 | 0.0035 | 0.0078 | 0.0036 | 0.0029 | 0.0021 | 0.0005 | 0.0008 | 0.0004 | 0.0008 | 0.0004 | 0.0002 | 0.0023 | 0.0059 | 0.0259 | 0.0351 | 0.0015 | 0.0028 |
Punctate Nuclear | 0.4341 | 0.398 | 0.4592 | 0.4001 | 0.2842 | 0.4206 | 0.3223 | 0.349 | 0.3589 | 0.4327 | 0.312 | 0.3649 | 0.2067 | 0.3425 | 0.1691 | 0.247 | 0.3287 | 0.3342 |
Vacuole | 0.0018 | 0.0049 | 0.0013 | 0.0012 | 0.0025 | 0.002 | 0.0009 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0017 | 0.0183 | 0.0019 | 0.0021 | 0.0005 | 0.0016 |
Vacuole Periphery | 0.0032 | 0.0023 | 0.0026 | 0.0026 | 0.0041 | 0.0017 | 0.0025 | 0.002 | 0.002 | 0.0018 | 0.0015 | 0.001 | 0.0093 | 0.009 | 0.0102 | 0.004 | 0.0021 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.7431 | 11.371 | 10.2331 | 9.8961 | 11.0533 | 9.1967 | 15.2889 | 13.7861 | 11.2037 | 13.698 |
Translational Efficiency | 1.0057 | 1.1006 | 0.9018 | 0.677 | 0.7926 | 0.8516 | 0.7286 | 0.7147 | 0.7546 | 0.6932 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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