Standard name
Human Ortholog
Description SIR protein involved in assembly of silent chromatin domains; silent information regulator (SIR) along with SIR2 and SIR3; involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; some alleles of SIR4 prolong lifespan; required for telomere hypercluster formation in quiescent yeast cells

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.08 0.09 0.09 0.13 0.13 0.12 0.09 0.05 0.06 0.08 0.26 0.31 0.31 0.27 0.24 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.5 0.3 0.09 0.06 0.29 0.05 0 0 0 0 0 0.28 0.35 0.2 0.24 0.31 0.36 0.41 0.28 0.3 0.28 0.37 0.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.27 0.51 0.75 0.43 0.74 0.82 0.84 0.92 0.88 0.89 0.14 0.11 0.28 0.12 0 0.15 0 0.08 0.11 0.07 0.06 0.07
Nucleus 0.13 0.17 0.11 0.08 0.05 0.1 0.07 0.1 0.08 0.07 0.07 0.14 0.12 0.1 0.16 0.19 0.13 0.05 0.11 0.05 0.08 0 0
Nuclear Periphery 0.08 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0.09 0.08 0.07 0.14 0.13 0.1 0.05 0.08 0.09
Nucleolus 0.08 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0.08 0.14 0.15 0.07 0.07 0.07 0.07 0 0
Peroxisomes 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.25 0.08 0.23 0.12 0.16 0.05 0.08 0.09 0 0 0.07 0.11 0.11 0.1 0.11 0.14 0.1 0.21 0.18 0.2 0.26 0.28 0.27
Vac/Vac Membrane 0.18 0.28 0.05 0.07 0.11 0 0 0 0 0 0 0.16 0.14 0.12 0.17 0.17 0.16 0 0.05 0.05 0.07 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 1 1 2 4
Bud 0 3 1 5 9 5 6 16 8 7 10 1 0 3 2 0 0 5 3 1 2 2 4
Bud Neck 1 0 2 0 1 0 1 2 1 0 0 0 2 0 1 0 0 1 2 3 2 1 3
Bud Site 0 0 3 5 1 1 10 10 3 5 14 0 0 0 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 20 31 20 23 32 25 37 30 11 14 23 44 62 69 46 24 19 5 9 2 6 7 6
Endoplasmic Reticulum 0 3 2 2 2 2 1 2 0 1 0 0 2 1 7 6 1 0 2 2 0 1 3
Endosome 152 119 20 15 74 10 8 2 2 3 4 47 70 45 41 32 35 196 152 104 67 124 111
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 1 1 0 3 2 1 2 0
Mitochondria 7 109 114 188 109 145 253 294 196 200 255 23 21 62 21 4 15 18 45 39 17 19 24
Nucleus 41 68 24 19 12 19 22 34 16 16 20 23 24 23 27 19 13 22 62 17 20 7 14
Nuclear Periphery 25 13 8 7 5 4 3 5 7 12 6 8 2 2 15 8 7 65 69 34 12 25 34
Nucleolus 25 9 4 4 5 5 4 12 3 12 11 9 4 7 14 14 15 31 39 23 16 10 15
Peroxisomes 1 2 1 0 15 0 0 0 0 0 1 2 5 2 0 0 2 2 2 6 2 6 5
SpindlePole 77 32 50 29 41 9 25 33 4 9 19 19 22 23 19 14 10 99 98 69 61 91 98
Vac/Vac Membrane 56 112 10 18 28 5 12 5 9 7 8 27 27 28 29 17 16 11 29 16 17 10 17
Unique Cell Count 304 397 222 250 254 196 309 349 213 226 288 167 199 225 169 102 98 484 548 346 242 331 365
Labelled Cell Count 405 502 259 315 334 230 382 445 260 286 371 203 241 265 226 139 135 484 548 346 242 331 365


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 4.4 3.7 3.5 3.9 3.5 3.3 3.2 2.8 3.0 2.8 4.5 4.7 4.3 6.8 7.4 6.9 4.7 4.9 5.2
Std Deviation (1e-4) 0.7 1.0 0.9 1.3 1.4 1.4 1.3 0.9 1.0 1.1 0.9 1.1 1.5 1.5 2.4 2.5 2.3 1.3 1.8 1.4
Intensity Change (Log2) -0.08 0.06 -0.1 -0.18 -0.21 -0.42 -0.29 -0.42 0.28 0.33 0.2 0.87 1.0 0.88 0.34 0.39 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 4.1 3.7 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4047 3.0021 2.6803 2.7947 2.4848 3.1309 -1.1993 -1.0745 -1.0847 -1.0415 -1.2074 -0.992 3.25 3.62 3.6033 4.7283 3.7564 3.622
Actin 0.0044 0.002 0.0061 0.0035 0.0039 0.0037 0.0005 0.0004 0.0004 0.0004 0.0004 0.0005 0.0676 0.0029 0.0116 0.0669 0.0022 0.0023
Bud 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0 0 0 0 0 0 0.001 0.0001 0.0003 0.0021 0 0.0001
Bud Neck 0.0008 0.0021 0.0015 0.0011 0.0106 0.0082 0.0003 0.0003 0.0004 0.0002 0.0004 0.0012 0.0008 0.0004 0.0004 0.0009 0.0006 0.0072
Bud Periphery 0.0003 0.0002 0.0002 0.0001 0.0004 0.0004 0 0 0 0 0 0 0.0027 0.0001 0.0008 0.0056 0 0.0003
Bud Site 0.0004 0.0024 0.0019 0.0004 0.0013 0.0004 0.0001 0.0001 0 0 0 0 0.0007 0.0015 0.001 0.0042 0.0001 0.0005
Cell Periphery 0.0001 0.0002 0.0001 0 0.0002 0.0001 0 0 0 0 0 0 0.0002 0.0001 0.0002 0.0002 0 0.0001
Cytoplasm 0.0102 0.0286 0.0188 0.0309 0.0411 0.0401 0.0036 0.0035 0.0011 0.0013 0.0021 0.0009 0.0054 0.0152 0.0067 0.0062 0.0112 0.0104
Cytoplasmic Foci 0.0158 0.0302 0.0333 0.0278 0.0236 0.0109 0.0028 0.003 0.0019 0.002 0.004 0.0006 0.0116 0.0211 0.015 0.012 0.0132 0.0093
Eisosomes 0 0.0001 0.0001 0 0.0001 0.0001 0 0 0 0 0 0 0.0002 0.0003 0.0004 0.0008 0.0002 0.0001
Endoplasmic Reticulum 0.0037 0.0101 0.0039 0.0083 0.0072 0.0076 0.0009 0.0013 0.0004 0.0002 0.0003 0.0003 0.0043 0.013 0.0039 0.0034 0.0064 0.0025
Endosome 0.0229 0.0303 0.0192 0.02 0.0308 0.0152 0.0054 0.0038 0.0017 0.004 0.0025 0.0012 0.0212 0.0313 0.0194 0.0336 0.008 0.0086
Golgi 0.003 0.003 0.0047 0.0026 0.0043 0.0017 0.0009 0.0005 0.0004 0.0004 0.0007 0.0004 0.0102 0.0047 0.0115 0.0183 0.0011 0.0018
Lipid Particles 0.0376 0.0389 0.0635 0.0351 0.0455 0.0115 0.0129 0.008 0.0061 0.0038 0.0265 0.0046 0.042 0.0922 0.0867 0.0489 0.0099 0.0304
Mitochondria 0.0013 0.0008 0.001 0.0005 0.0011 0.0019 0.0003 0.0002 0.0002 0.0002 0.0003 0.0002 0.0042 0.0008 0.0309 0.0763 0.0003 0.0005
None 0.0042 0.0339 0.017 0.0335 0.0095 0.0216 0.002 0.0044 0.0007 0.0009 0.0011 0.0004 0.007 0.0717 0.0626 0.0067 0.1965 0.0133
Nuclear Periphery 0.3395 0.2379 0.2336 0.3215 0.3818 0.2549 0.5158 0.4173 0.4524 0.3892 0.4351 0.3963 0.574 0.3099 0.5067 0.1088 0.3816 0.3552
Nucleolus 0.0102 0.024 0.014 0.004 0.0609 0.0289 0.0157 0.0278 0.0207 0.0098 0.0502 0.0454 0.008 0.0056 0.0057 0.016 0.0075 0.0556
Nucleus 0.1042 0.1465 0.1101 0.103 0.0838 0.1662 0.1125 0.1767 0.1521 0.1519 0.1622 0.1819 0.019 0.0534 0.0292 0.3009 0.0282 0.1613
Peroxisomes 0.0024 0.0035 0.0078 0.0036 0.0029 0.0021 0.0005 0.0008 0.0004 0.0008 0.0004 0.0002 0.0023 0.0059 0.0259 0.0351 0.0015 0.0028
Punctate Nuclear 0.4341 0.398 0.4592 0.4001 0.2842 0.4206 0.3223 0.349 0.3589 0.4327 0.312 0.3649 0.2067 0.3425 0.1691 0.247 0.3287 0.3342
Vacuole 0.0018 0.0049 0.0013 0.0012 0.0025 0.002 0.0009 0.0006 0.0002 0.0002 0.0002 0.0002 0.0017 0.0183 0.0019 0.0021 0.0005 0.0016
Vacuole Periphery 0.0032 0.0023 0.0026 0.0026 0.0041 0.0017 0.0025 0.002 0.002 0.0018 0.0015 0.001 0.0093 0.009 0.0102 0.004 0.0021 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.7431 11.371 10.2331 9.8961 11.0533 9.1967 15.2889 13.7861 11.2037 13.698
Translational Efficiency 1.0057 1.1006 0.9018 0.677 0.7926 0.8516 0.7286 0.7147 0.7546 0.6932

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description SIR protein involved in assembly of silent chromatin domains; silent information regulator (SIR) along with SIR2 and SIR3; involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; some alleles of SIR4 prolong lifespan; required for telomere hypercluster formation in quiescent yeast cells
Localization
Cell Percentages nucleus (25%), nuclear periphery (20%), mixed (35%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sir4

Sir4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sir4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available