Standard name | |
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Human Ortholog | |
Description | Reticulon protein; involved in nuclear pore assembly and maintenance of tubular ER morphology; promotes membrane curvature; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; increases tubular ER when overexpressed; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; member of the RTNLA subfamily |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 18 | 9 | 10 | 9 | 28 | 49 | 49 | 35 | 73 | 77 | 112 | 11 | 9 | 11 | 10 | 4 | 2 | 33 | 32 | 27 | 8 | 26 | 34 |
Bud | 7 | 8 | 5 | 3 | 5 | 5 | 15 | 15 | 12 | 22 | 20 | 18 | 1 | 5 | 5 | 9 | 2 | 1 | 0 | 4 | 0 | 2 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 12 | 54 | 27 | 26 | 23 | 20 | 32 | 32 | 22 | 33 | 28 | 48 | 47 | 82 | 79 | 35 | 39 | 20 | 20 | 47 | 23 | 11 | 23 | 23 |
Cytoplasm | 11 | 23 | 6 | 8 | 10 | 17 | 17 | 17 | 21 | 12 | 16 | 14 | 4 | 6 | 8 | 3 | 23 | 4 | 5 | 30 | 3 | 5 | 10 | 3 |
Endoplasmic Reticulum | 68 | 119 | 49 | 59 | 80 | 156 | 185 | 194 | 107 | 131 | 103 | 135 | 74 | 88 | 95 | 8 | 8 | 4 | 135 | 155 | 46 | 32 | 89 | 85 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 |
Golgi | 23 | 10 | 24 | 20 | 40 | 40 | 84 | 82 | 52 | 102 | 77 | 138 | 10 | 18 | 20 | 31 | 47 | 33 | 9 | 15 | 2 | 2 | 13 | 14 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 28 | 22 | 34 | 39 | 0 | 0 | 1 | 15 | 42 | 8 | 7 | 15 | 5 | 7 | 9 | 6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 1 | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 9 | 0 | 0 | 1 | 0 |
Unique Cell Count | 113 | 210 | 96 | 103 | 140 | 217 | 285 | 288 | 187 | 257 | 236 | 318 | 117 | 181 | 177 | 95 | 131 | 61 | 222 | 318 | 113 | 74 | 181 | 179 |
Labelled Cell Count | 124 | 234 | 121 | 126 | 169 | 267 | 386 | 393 | 278 | 399 | 359 | 507 | 147 | 208 | 220 | 114 | 165 | 74 | 222 | 318 | 113 | 74 | 181 | 179 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 91.7 | 78.9 | 72.2 | 68.3 | 61.1 | 48.6 | 43.7 | 43.7 | 35.4 | 35.9 | 34.0 | 35.0 | 81.2 | 78.2 | 76.0 | 62.4 | 74.6 | 88.3 | 71.4 | 74.7 | 79.0 |
Std Deviation (1e-4) | 22.0 | 20.7 | 19.7 | 16.0 | 14.8 | 12.3 | 9.9 | 9.9 | 9.0 | 8.9 | 7.3 | 7.3 | 19.5 | 20.8 | 19.6 | 20.1 | 29.1 | 29.1 | 22.7 | 23.6 | 23.8 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.24 | -0.57 | -0.72 | -0.72 | -1.03 | -1.01 | -1.09 | -1.05 | 0.17 | 0.12 | 0.07 | -0.21 | 0.05 | 0.29 | -0.02 | 0.05 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.1 | -0.8 | 0.9 | 1.8 | 1.8 | 2.1 | 3.8 | 4.4 | 4.9 | 0 | -1.4 | -1.0 | 0.3 | -2.0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0.4 | 1.1 | 1.0 | 0.2 | 0 | 0 | 0 | 1.1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.5 | -2.2 | -4.3 | -4.0 | -4.0 | -3.4 | -3.4 | -3.6 | -2.9 | 1.8 | 2.8 | 2.7 | 1.3 | 0.3 | 0.6 |
Cytoplasm | 0.4 | 0.3 | 0.5 | -0.1 | -0.1 | 1.4 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 |
Endoplasmic Reticulum | 0.9 | 0.9 | 3.6 | 2.4 | 2.9 | 1.0 | 0 | -1.2 | -1.5 | 1.8 | -0.4 | 0.4 | -6.4 | -7.7 | -5.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -0.9 | 0.6 | -1.3 | 0.8 | 0.7 | 0.5 | 2.6 | 1.4 | 3.2 | -3.3 | -3.3 | -2.9 | 1.2 | 1.7 | 3.7 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.0 | 3.0 | 3.9 | 3.6 | 0 | 0 | 0 | 4.1 | 6.1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 80.7656 | 86.506 | 78.8437 | 78.6783 | 71.5718 | 81.9994 | 77.6579 | 86.5182 | 76.0469 | 82.307 | 63.1179 | 83.8709 | 90.1822 | 94.4281 | 93.1449 | 91.2368 | 87.6449 | 92.9237 |
Actin | 0.0327 | 0.0071 | 0.0407 | 0.0265 | 0.0251 | 0.0117 | 0.0725 | 0.0082 | 0.042 | 0.0335 | 0.062 | 0.02 | 0.057 | 0.0089 | 0.0128 | 0.0013 | 0.0184 | 0.003 |
Bud | 0.0015 | 0.0007 | 0.0018 | 0.0003 | 0.0007 | 0.0003 | 0.0038 | 0.0008 | 0.0023 | 0.0012 | 0.0041 | 0.0004 | 0.0005 | 0.0005 | 0.0007 | 0.0001 | 0.0011 | 0.0001 |
Bud Neck | 0.0078 | 0.0044 | 0.0076 | 0.0029 | 0.003 | 0.0154 | 0.0113 | 0.0069 | 0.0086 | 0.0227 | 0.0575 | 0.0243 | 0.0057 | 0.0042 | 0.0065 | 0.0044 | 0.0081 | 0.0117 |
Bud Periphery | 0.0051 | 0.0022 | 0.01 | 0.0021 | 0.0051 | 0.0017 | 0.0154 | 0.0034 | 0.0083 | 0.0086 | 0.0246 | 0.0031 | 0.0022 | 0.0035 | 0.0045 | 0.0009 | 0.0054 | 0.0014 |
Bud Site | 0.0075 | 0.0309 | 0.0081 | 0.0007 | 0.0007 | 0.0019 | 0.0354 | 0.04 | 0.0187 | 0.0022 | 0.0094 | 0.0004 | 0.0024 | 0.0213 | 0.0036 | 0.0003 | 0.0018 | 0.0002 |
Cell Periphery | 0.1265 | 0.2123 | 0.1944 | 0.1667 | 0.0792 | 0.0686 | 0.1758 | 0.2734 | 0.1716 | 0.2383 | 0.1882 | 0.1585 | 0.131 | 0.2554 | 0.1738 | 0.1561 | 0.0876 | 0.1551 |
Cytoplasm | 0.0151 | 0.0008 | 0.0008 | 0.0003 | 0.0004 | 0.0002 | 0.011 | 0.0012 | 0.0114 | 0.0006 | 0.0029 | 0.001 | 0.0011 | 0.0013 | 0.0005 | 0.0002 | 0.0003 | 0.0003 |
Cytoplasmic Foci | 0.0253 | 0.0004 | 0.0003 | 0.0011 | 0.0396 | 0.0018 | 0.0407 | 0.0002 | 0.0081 | 0.0002 | 0.0056 | 0.0046 | 0.001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Eisosomes | 0.5966 | 0.6251 | 0.5927 | 0.5648 | 0.7336 | 0.7738 | 0.4818 | 0.5659 | 0.5458 | 0.5846 | 0.4881 | 0.6313 | 0.6513 | 0.5494 | 0.6385 | 0.6591 | 0.7755 | 0.6895 |
Endoplasmic Reticulum | 0.0266 | 0.0414 | 0.0771 | 0.1129 | 0.0168 | 0.0413 | 0.0319 | 0.0487 | 0.0637 | 0.0418 | 0.0138 | 0.093 | 0.044 | 0.0867 | 0.08 | 0.1256 | 0.0301 | 0.0868 |
Endosome | 0.0063 | 0.0011 | 0.0008 | 0.0047 | 0.0003 | 0.0023 | 0.0088 | 0.0003 | 0.0078 | 0.0015 | 0.009 | 0.0013 | 0.005 | 0.0006 | 0.0011 | 0.0006 | 0.0005 | 0.0007 |
Golgi | 0.017 | 0.0132 | 0.0075 | 0.017 | 0.0038 | 0.0367 | 0.0095 | 0.0018 | 0.0086 | 0.0106 | 0.0112 | 0.0253 | 0.0116 | 0.0044 | 0.0093 | 0.0133 | 0.0062 | 0.0196 |
Lipid Particles | 0.014 | 0.0039 | 0.0034 | 0.0056 | 0.0026 | 0.0066 | 0.0145 | 0.0023 | 0.005 | 0.0046 | 0.0075 | 0.002 | 0.0058 | 0.0059 | 0.0046 | 0.0022 | 0.0018 | 0.0015 |
Mitochondria | 0.0509 | 0.01 | 0.0143 | 0.0651 | 0.058 | 0.0179 | 0.0305 | 0.009 | 0.0543 | 0.013 | 0.0758 | 0.0072 | 0.0337 | 0.0131 | 0.0326 | 0.0051 | 0.0282 | 0.0089 |
None | 0.0043 | 0.0007 | 0.0034 | 0.001 | 0.0061 | 0.0007 | 0.0094 | 0.0009 | 0.0072 | 0.0008 | 0.0097 | 0.0046 | 0.0024 | 0.0011 | 0.0013 | 0.0009 | 0.0013 | 0.0008 |
Nuclear Periphery | 0.0182 | 0.0285 | 0.0228 | 0.0124 | 0.0051 | 0.0088 | 0.0209 | 0.024 | 0.0178 | 0.0174 | 0.0073 | 0.0138 | 0.0229 | 0.0275 | 0.0171 | 0.0224 | 0.0113 | 0.0144 |
Nucleolus | 0.0045 | 0.0009 | 0.001 | 0.0006 | 0.0141 | 0.0008 | 0.0035 | 0.0009 | 0.0026 | 0.0008 | 0.0024 | 0.0003 | 0.0064 | 0.0007 | 0.0007 | 0.0003 | 0.0058 | 0.0002 |
Nucleus | 0.0016 | 0.0008 | 0.0008 | 0.0004 | 0.0008 | 0.0005 | 0.0017 | 0.0008 | 0.0011 | 0.0008 | 0.0021 | 0.0005 | 0.0017 | 0.0007 | 0.0006 | 0.0003 | 0.0017 | 0.0003 |
Peroxisomes | 0.0191 | 0.0004 | 0.0004 | 0.0017 | 0.0017 | 0.0035 | 0.0027 | 0.0003 | 0.0021 | 0.0003 | 0.004 | 0.0003 | 0.001 | 0.0003 | 0.0012 | 0.0002 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.0012 | 0.0002 | 0.0001 | 0.0001 | 0.001 | 0.0007 | 0.0029 | 0.0001 | 0.0003 | 0.0001 | 0.0011 | 0.0002 | 0.002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Vacuole | 0.0133 | 0.0129 | 0.0092 | 0.0099 | 0.0016 | 0.0027 | 0.0132 | 0.0098 | 0.0097 | 0.0122 | 0.0109 | 0.0053 | 0.0086 | 0.0123 | 0.008 | 0.0051 | 0.0108 | 0.0036 |
Vacuole Periphery | 0.0049 | 0.0021 | 0.0028 | 0.0034 | 0.0007 | 0.0021 | 0.0029 | 0.0012 | 0.0031 | 0.0041 | 0.0027 | 0.0027 | 0.0029 | 0.0019 | 0.0024 | 0.0015 | 0.0035 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 319.9624 | 328.7921 | 331.3484 | 369.9557 | 294.8221 | 265.7633 | 334.9683 | 309.2743 | 273.8126 | 336.5165 |
Translational Efficiency | 1.5297 | 1.5218 | 1.559 | 1.1757 | 1.425 | 1.9642 | 1.5315 | 1.4305 | 1.8564 | 1.535 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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