Standard name | |
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Human Ortholog | |
Description | Homoaconitase; catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 3 | 25 | 34 | 16 | 7 | 14 | 12 | 3 | – | 18 | 12 | 5 | 8 | 10 | 5 | 2 | 7 | 5 | 13 | 25 | 5 | 39 | 41 |
Bud | 1 | 0 | 2 | 5 | 7 | 1 | 8 | 4 | 5 | – | 10 | 9 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 4 | 5 | 6 | 4 | 1 | 3 | 2 | 2 | – | 9 | 9 | 3 | 2 | 3 | 1 | 1 | 5 | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 0 | 0 | 1 | 5 | 6 | 5 | 9 | 5 | 3 | – | 9 | 8 | 1 | 3 | 9 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | – | 8 | 5 | 2 | 1 | 5 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 2 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 2 |
Golgi | 3 | 8 | 103 | 72 | 153 | 20 | 47 | 65 | 10 | – | 24 | 67 | 2 | 22 | 19 | 40 | 10 | 35 | 14 | 42 | 58 | 11 | 30 | 30 |
Mitochondria | 222 | 182 | 285 | 343 | 442 | 499 | 662 | 417 | 504 | – | 724 | 629 | 345 | 446 | 488 | 3 | 1 | 4 | 206 | 147 | 300 | 63 | 228 | 263 |
Nucleus | 0 | 1 | 1 | 0 | 1 | 3 | 3 | 3 | 1 | – | 2 | 1 | 1 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 1 | 2 | 1 | 2 | 12 | 21 | 20 | 17 | – | 43 | 34 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 4 |
Nucleolus | 53 | 36 | 32 | 32 | 70 | 111 | 171 | 155 | 100 | – | 106 | 75 | 46 | 27 | 32 | 11 | 4 | 7 | 4 | 4 | 4 | 4 | 7 | 8 |
Peroxisomes | 0 | 3 | 3 | 1 | 4 | 1 | 4 | 3 | 0 | – | 4 | 5 | 0 | 0 | 3 | 0 | 0 | 0 | 3 | 6 | 7 | 1 | 30 | 36 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 14 | 16 | 31 | 28 | 51 | 34 | 36 | 53 | 22 | – | 19 | 20 | 20 | 36 | 26 | 10 | 2 | 8 | 2 | 6 | 5 | 1 | 2 | 8 |
Unique Cell Count | 235 | 207 | 367 | 385 | 561 | 581 | 804 | 627 | 567 | 827 | 724 | 356 | 475 | 528 | 64 | 19 | 56 | 242 | 233 | 410 | 97 | 353 | 413 | |
Labelled Cell Count | 302 | 255 | 491 | 528 | 757 | 697 | 980 | 741 | 672 | 978 | 882 | 426 | 549 | 597 | 71 | 21 | 72 | 242 | 233 | 410 | 97 | 353 | 413 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 46.9 | 56.0 | 46.0 | 48.9 | 44.0 | 33.5 | 31.0 | 28.8 | 24.8 | – | 20.9 | 22.3 | 59.2 | 57.2 | 49.5 | 59.2 | 56.4 | 59.7 | 42.0 | 46.7 | 46.3 |
Std Deviation (1e-4) | 12.3 | 17.0 | 12.0 | 13.9 | 11.5 | 10.0 | 9.5 | 8.0 | 7.6 | – | 6.5 | 6.5 | 14.3 | 14.2 | 13.6 | 19.9 | 15.5 | 20.9 | 15.0 | 16.9 | 17.8 |
Intensity Change (Log2) | – | – | – | 0.09 | -0.07 | -0.46 | -0.57 | -0.68 | -0.89 | – | -1.14 | -1.05 | 0.36 | 0.31 | 0.1 | 0.36 | 0.29 | 0.37 | -0.13 | 0.02 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.0 | -2.9 | -4.7 | -4.5 | -3.9 | -5.5 | – | -4.0 | -4.4 | -3.6 | -3.8 | -3.7 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -3.0 | -0.3 | -11.0 | -10.6 | -7.2 | -12.0 | – | -13.0 | -8.1 | -10.5 | -9.5 | -10.5 | 5.4 | 2.3 | 5.1 |
Mitochondria | 4.2 | 0.4 | 3.3 | 1.9 | -3.7 | 4.6 | – | 4.4 | 3.9 | 7.7 | 6.9 | 6.3 | -11.4 | -7.0 | -10.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1.9 | 2.4 | 2.7 | 2.6 | – | 3.9 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.2 | 1.8 | 4.4 | 5.3 | 6.2 | 3.8 | – | 2.0 | 0.9 | 1.8 | -1.7 | -1.5 | 2.1 | 0 | 0.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.6 | 0.3 | -1.5 | -2.7 | 0 | -2.9 | – | -4.9 | -4.2 | -1.5 | -0.5 | -2.1 | 1.8 | 0 | 1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 84.1282 | 95.5795 | 84.3185 | 90.4368 | 78.2061 | 87.5043 | 96.5007 | 106.6251 | 101.804 | 68.031 | 91.2089 | 108.577 | 86.0092 | 103.8651 | 93.9794 | 90.1416 | 95.659 | 91.0307 |
Actin | 0.0025 | 0.0004 | 0.0113 | 0.0007 | 0.0022 | 0.0003 | 0.0084 | 0.0011 | 0.0008 | 0.0004 | 0.0143 | 0.0002 | 0.016 | 0.0016 | 0.0095 | 0.0148 | 0.0011 | 0.008 |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0015 | 0 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0 | 0.0001 |
Bud Neck | 0.0005 | 0 | 0.0015 | 0 | 0.008 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0.0005 | 0.0017 | 0 | 0 |
Bud Periphery | 0.0001 | 0 | 0.0002 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0014 | 0 | 0.0001 | 0.0003 | 0.0003 | 0.0004 | 0 | 0.0002 |
Bud Site | 0.0003 | 0 | 0.0059 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0234 | 0 | 0.0004 | 0.0007 | 0.0043 | 0.022 | 0 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0003 | 0 | 0.0011 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0 | 0.0006 | 0.0003 | 0.0003 | 0.0008 | 0.0001 | 0.0001 |
Cytoplasm | 0.0012 | 0 | 0.0006 | 0 | 0.001 | 0 | 0.0003 | 0 | 0.0001 | 0.0012 | 0.0004 | 0 | 0.0021 | 0 | 0.0005 | 0.0028 | 0 | 0.0001 |
Cytoplasmic Foci | 0.0047 | 0 | 0.0028 | 0 | 0.0181 | 0 | 0.0065 | 0.0001 | 0.006 | 0.022 | 0.0197 | 0 | 0.0185 | 0.0032 | 0.0087 | 0.005 | 0.002 | 0.0015 |
Eisosomes | 0.0028 | 0.0013 | 0.0066 | 0.0023 | 0.0019 | 0.0005 | 0.0021 | 0.0021 | 0.0029 | 0.0035 | 0.0005 | 0.0006 | 0.0019 | 0.0013 | 0.0041 | 0.001 | 0.0005 | 0.0005 |
Endoplasmic Reticulum | 0.0011 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0 | 0.0004 | 0.0003 | 0 | 0.002 | 0 | 0.001 | 0.0023 | 0 | 0.0004 |
Endosome | 0.0066 | 0.0001 | 0.0041 | 0 | 0.0157 | 0.0004 | 0.0016 | 0.0001 | 0.0002 | 0.0238 | 0.0042 | 0 | 0.0064 | 0.0001 | 0.0044 | 0.0041 | 0 | 0.0038 |
Golgi | 0.0023 | 0.0003 | 0.0016 | 0.0001 | 0.0044 | 0.0002 | 0.003 | 0.0006 | 0.0005 | 0.0038 | 0.0045 | 0.0001 | 0.0034 | 0.0007 | 0.0019 | 0.0035 | 0 | 0.0043 |
Lipid Particles | 0.0082 | 0.0055 | 0.0076 | 0.001 | 0.0048 | 0.0059 | 0.007 | 0.0023 | 0.0051 | 0.0071 | 0.016 | 0.0058 | 0.011 | 0.0039 | 0.01 | 0.0274 | 0.0013 | 0.0049 |
Mitochondria | 0.9242 | 0.9833 | 0.935 | 0.9942 | 0.9277 | 0.95 | 0.9415 | 0.987 | 0.9529 | 0.8929 | 0.8742 | 0.9814 | 0.8863 | 0.9707 | 0.93 | 0.8919 | 0.9781 | 0.9694 |
None | 0.0065 | 0 | 0.0029 | 0 | 0.0003 | 0 | 0.0011 | 0 | 0.0017 | 0.0059 | 0.0005 | 0 | 0.0033 | 0.0001 | 0.0006 | 0.0009 | 0.0001 | 0.0004 |
Nuclear Periphery | 0.0033 | 0 | 0.0014 | 0 | 0.0002 | 0.0121 | 0.0005 | 0 | 0.0002 | 0.0007 | 0.0009 | 0 | 0.0091 | 0 | 0.0019 | 0.0023 | 0 | 0.0014 |
Nucleolus | 0.0135 | 0.0002 | 0.0016 | 0 | 0.0007 | 0.0002 | 0.0166 | 0.0004 | 0.0089 | 0.0229 | 0.0081 | 0.0001 | 0.0197 | 0.0022 | 0.0015 | 0.0003 | 0.0124 | 0.001 |
Nucleus | 0.004 | 0 | 0.0007 | 0 | 0.0004 | 0.0009 | 0.0011 | 0 | 0.0004 | 0.0015 | 0.0003 | 0 | 0.0018 | 0.0001 | 0.0006 | 0.0008 | 0.0003 | 0.0002 |
Peroxisomes | 0.0034 | 0.0013 | 0.006 | 0.0006 | 0.0051 | 0.0003 | 0.0034 | 0.0014 | 0.0081 | 0.0018 | 0.0186 | 0.0004 | 0.0075 | 0.0029 | 0.0049 | 0.0029 | 0.0023 | 0.0016 |
Punctate Nuclear | 0.0037 | 0 | 0.0033 | 0 | 0.0024 | 0.0002 | 0.0018 | 0 | 0.0002 | 0.001 | 0.0087 | 0 | 0.0036 | 0 | 0.0018 | 0.0029 | 0 | 0.0005 |
Vacuole | 0.001 | 0.0004 | 0.0005 | 0 | 0.002 | 0.0003 | 0.0005 | 0.0006 | 0.0015 | 0.005 | 0.0004 | 0.0001 | 0.0015 | 0.0011 | 0.0011 | 0.0008 | 0.0002 | 0.0002 |
Vacuole Periphery | 0.0096 | 0.0068 | 0.0058 | 0.0009 | 0.0024 | 0.0283 | 0.0035 | 0.004 | 0.0098 | 0.0045 | 0.0015 | 0.0112 | 0.0044 | 0.0103 | 0.0117 | 0.0112 | 0.0013 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 177.2761 | 179.5565 | 144.8399 | 124.771 | 147.0466 | 149.3697 | 162.628 | 134.9809 | 131.6359 | 124.369 |
Translational Efficiency | 1.0441 | 1.0834 | 0.9994 | 1.1468 | 0.9672 | 1.3005 | 1.0461 | 1.1795 | 0.9165 | 1.0022 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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