Standard name
Human Ortholog
Description Essential beta-coat protein of the COPI coatomer; involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.13 0.16 0.13 0.15 0.12 0.05 0.08 0.08 – 0.08 0.1 0.12 0.11 0.1 0.07 – 0.16 – 0 0 0.06 0.05 0 0.07
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0 0 0 0 0 – 0 0.05 0.06 0 0 0.05 – 0.07 – 0 0 0 0 0 0
Endoplasmic Reticulum 0.09 0.11 0.05 0 0 0 0 0 – 0 0.05 0.1 0.1 0.12 0.1 – 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Golgi 0.88 0.61 0.78 0.8 0.83 0.3 0.36 0.24 – 0.19 0.22 0.36 0.88 0.86 0.84 – 0.82 – 0.88 0.79 0.89 0.86 0.91 0.88
Mitochondria 0.22 0.41 0.62 0.5 0.53 0.85 0.78 0.8 – 0.8 0.81 0.66 0.18 0.18 0 – 0.09 – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.1 – 0.07 0.05 0 0 0 0 – 0.06 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.11 0.05 0.05 0.1 0.05 0.05 0 – 0.05 0 0 0.15 0.15 0.2 – 0.1 – 0 0.09 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 42 38 43 35 49 18 52 60 – 33 31 45 18 17 15 – 11 – 7 11 21 11 12 27
Bud 1 1 5 1 2 3 3 4 – 5 5 6 4 4 0 – 0 – 0 1 2 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 1 2 0 0 0 0 – 0 – – – – – – –
Cell Periphery 2 1 0 0 4 1 1 2 – 1 0 1 0 0 1 – 2 – 0 0 0 0 0 0
Cytoplasm 10 18 9 5 8 9 12 14 – 12 16 24 6 6 10 – 5 – 2 3 0 0 0 0
Endoplasmic Reticulum 29 26 16 9 9 15 29 29 – 19 15 39 17 20 20 – 2 – 5 4 1 0 0 2
Endosome 0 1 2 0 0 0 4 2 – 6 3 0 0 0 0 – 3 – 5 4 3 4 2 2
Golgi 274 149 269 184 332 118 238 171 – 82 69 136 143 141 173 – 55 – 282 235 310 210 286 330
Mitochondria 70 101 213 115 213 336 521 577 – 345 258 249 29 29 9 – 6 – 3 5 4 7 3 3
Nucleus 0 0 0 0 0 1 1 2 – 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 1 1 4 1 – 3 1 1 0 0 0 – 0 – 0 0 0 1 1 0
Nucleolus 3 3 9 6 4 10 27 71 – 31 17 15 0 0 1 – 4 – 0 1 0 0 1 0
Peroxisomes 0 0 0 0 1 1 0 0 – 0 0 0 0 0 0 – 0 – 0 0 1 1 1 1
SpindlePole 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 – 0 – 1 0 0 0 0 0
Vac/Vac Membrane 35 26 16 11 41 18 34 26 – 22 6 14 25 24 42 – 7 – 9 27 1 4 1 4
Unique Cell Count 313 244 343 230 399 397 665 717 429 318 380 163 164 207 67 320 298 350 243 314 376
Labelled Cell Count 467 364 582 366 665 531 926 959 560 423 530 242 241 271 95 320 298 350 243 314 376


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 33.1 36.2 26.6 27.1 25.5 22.5 21.9 21.4 – 19.0 18.7 20.7 29.8 29.8 34.6 – 25.9 – 34.8 36.3 38.7
Std Deviation (1e-4) 7.2 8.5 5.4 6.3 4.9 5.1 4.7 5.3 – 5.0 4.9 5.4 6.9 6.9 8.3 – 9.1 – 6.9 7.1 8.6
Intensity Change (Log2) – – – 0.02 -0.06 -0.24 -0.28 -0.32 – -0.48 -0.51 -0.37 0.16 0.16 0.38 – -0.04 – 0.39 0.45 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.9 -0.1 -3.9 -2.4 -2.1 – -2.2 -1.1 -0.3 -0.5 -0.7 -2.0 – 0.9 –
Bud 0 0 0 0 0 – 0 0 0.1 0 0 0 – 0 –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – 0 –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – 0 –
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – 0 –
Cytoplasm -0.3 -0.6 -0.3 -0.9 -0.7 – 0.1 1.6 2.4 0 0 1.4 – 0 –
Endoplasmic Reticulum -0.4 -1.8 -0.6 -0.2 -0.5 – -0.2 0 2.8 2.5 3.1 2.3 – 0 –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – 0 –
Golgi 0.5 1.7 -13.2 -12.8 -16.9 – -16.4 -14.6 -11.5 2.5 2.0 1.5 – 0.7 –
Mitochondria -2.9 -2.4 7.0 5.5 6.4 – 5.7 5.4 1.0 -9.3 -9.4 -13.4 – -8.0 –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – 0 –
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – 0 –
Nucleolus 0 -1.7 -0.1 1.2 4.2 – 2.9 1.8 1.0 0 0 0 – 0 –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – 0 –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – 0 –
Vacuole 0.1 2.9 -0.1 0.3 -0.8 – 0.3 -2.0 -0.7 4.1 3.9 5.8 – 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 30.8731 35.0418 34.4567 33.4152 31.4748 33.234 27.5656 37.947 32.3253 32.6577 31.5469 33.3894 28.3712 36.4666 34.1854 34.8427 28.0133 32.7453
Actin 0.0276 0.0075 0.0045 0.005 0.0003 0.0009 0.0397 0.0029 0.0157 0.0173 0.0009 0.0008 0.0435 0.0164 0.0398 0.0059 0.0377 0.0054
Bud 0.0003 0.0002 0.0001 0.0007 0.0001 0 0.0002 0 0.0002 0.0001 0 0 0.0004 0.0002 0.0003 0.0001 0.0009 0.0001
Bud Neck 0.0002 0.0002 0.0001 0.0001 0 0.0001 0.0002 0 0.0001 0.0001 0 0 0.0009 0.0001 0.0002 0.0001 0.0002 0.0001
Bud Periphery 0.0004 0.0002 0.0002 0.0002 0.0001 0 0.0004 0 0.0002 0.0001 0 0 0.0006 0.0002 0.0003 0.0002 0.001 0.0001
Bud Site 0.0002 0.0002 0.0001 0.0001 0 0 0.0004 0 0.0007 0 0 0 0.0042 0.0002 0.0003 0.0001 0.0018 0
Cell Periphery 0.0003 0.0003 0.0003 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0001 0 0 0.0006 0.0002 0.0001 0.0002 0.0001 0.0001
Cytoplasm 0.002 0.003 0.0005 0.0043 0.0003 0.0006 0.0007 0.0004 0.0007 0.0003 0.0001 0.0001 0.0095 0.0005 0.0005 0.0024 0.0008 0.0004
Cytoplasmic Foci 0.0085 0.0146 0.0066 0.0135 0.0064 0.0045 0.0111 0.0066 0.0097 0.0042 0.005 0.0026 0.0202 0.0066 0.0057 0.0203 0.0106 0.0032
Eisosomes 0.0004 0.0001 0.0001 0 0 0.0001 0.0005 0.0002 0.0002 0.0003 0 0.0001 0.0008 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0009 0.0009 0.0006 0.0005 0.0005 0.0007 0.0004 0.0003 0.0005 0.0007 0.0003 0.0002 0.0082 0.0005 0.0004 0.0031 0.0008 0.0006
Endosome 0.1101 0.1349 0.0892 0.1609 0.1033 0.0624 0.0555 0.0622 0.0736 0.1002 0.0651 0.0289 0.0712 0.0851 0.0671 0.1199 0.0566 0.0532
Golgi 0.751 0.7713 0.8122 0.7601 0.7923 0.902 0.7956 0.901 0.8233 0.8049 0.8999 0.9348 0.6647 0.8295 0.7915 0.7138 0.8728 0.8249
Lipid Particles 0.0043 0.0053 0.0054 0.0025 0.0193 0.0023 0.0091 0.0002 0.0057 0.0033 0.0013 0.0007 0.0171 0.0042 0.0053 0.0194 0.0031 0.0028
Mitochondria 0.054 0.0385 0.0403 0.0065 0.0459 0.013 0.0591 0.0179 0.0462 0.0384 0.0211 0.0295 0.111 0.0185 0.0737 0.026 0.0075 0.0841
None 0.0001 0 0 0 0 0 0.0004 0 0.0013 0 0 0 0.0038 0 0 0.0019 0.0003 0
Nuclear Periphery 0.001 0.0006 0.0029 0.0004 0.0014 0.0003 0.0005 0.0001 0.0006 0.0004 0.0001 0 0.005 0.0002 0.0002 0.0018 0.0004 0.0006
Nucleolus 0.0002 0 0.0002 0 0 0 0.0022 0 0.0004 0 0 0 0.0005 0 0.0001 0.0001 0 0.0001
Nucleus 0.0003 0.0001 0.0002 0 0.0001 0 0.0025 0 0.0003 0 0 0 0.0009 0.0001 0 0.0002 0.0001 0.0001
Peroxisomes 0.0002 0.0002 0.0001 0.0004 0.0001 0 0.0024 0.0001 0.0019 0.0002 0.0001 0.0001 0.005 0.0001 0.0003 0.0012 0.0024 0.0001
Punctate Nuclear 0 0 0 0 0 0 0.0015 0 0.002 0 0 0 0.0016 0 0 0.0011 0.0004 0
Vacuole 0.0093 0.0068 0.0081 0.0107 0.0088 0.0036 0.0052 0.0028 0.0055 0.0053 0.0006 0.0002 0.0102 0.0111 0.0017 0.0128 0.0005 0.0053
Vacuole Periphery 0.0287 0.0151 0.0281 0.0339 0.0208 0.0094 0.0121 0.0053 0.0112 0.024 0.0054 0.0019 0.0201 0.0263 0.0124 0.0694 0.0019 0.0187

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 166.3414 120.8496 108.4625 132.6498 137.3329 140.0193 126.7134 124.1667 120.7898 152.7192
Translational Efficiency 1.0051 1.2579 1.1314 0.988 0.9385 1.0389 1.0958 0.905 0.947 0.9274

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential beta-coat protein of the COPI coatomer; involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
Localization
Cell Percentages nucleus (11%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec26

Sec26


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec26-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available