Standard name
Human Ortholog
Description Peroxisomal membrane signal receptor for peroxisomal matrix proteins; receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins; required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – – 0 0 0.11 0 0 0 0.08
Bud 0.15 0.08 0.11 0.16 0.11 0.12 0.12 0.09 0.11 0.13 0.15 0.1 0.05 0 0.05 – – 0.06 0.06 0.06 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – – 0 0.05 0 0 0 0 0
Bud Site 0.15 0.08 0.07 0.09 0.07 0.07 0.05 0.06 0.09 0 0.05 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.05 0.08 0.08 0.15 0.25 0.31 0.34 0.31 0.27 0.31 0.16 0.25 0.36 – – 0.31 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Endosome 0.1 0.23 0.16 0.14 0.17 0.08 0.07 0.05 0 0 0 0 0 0 0 – – 0.06 0.09 0.13 0.13 0.16 0.13 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.45 0.36 0.12 0.12 0.12 0.22 0.22 0.26 0.34 0.4 0.44 0.44 0 0 0 – – 0.19 0.05 0 0 0.05 0.06 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0.4 0.56 0.56 0.54 0.46 0.33 0.18 0.13 0.15 0.14 0.13 0.14 0.23 0.25 0.16 – – 0.25 0.48 0.42 0.55 0.5 0.5 0.44
SpindlePole 0.25 0.18 0.52 0.52 0.57 0.55 0.49 0.41 0.36 0.33 0.29 0.3 0.66 0.53 0.48 – – 0.25 0.18 0.17 0.19 0.17 0.12 0.16
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 2 3 1 1 0 0 0 0 2 0 11 10 21 – – 0 0 16 10 4 9 21
Bud 3 10 38 49 37 60 60 40 47 59 64 45 20 14 18 – – 1 3 8 10 2 5 11
Bud Neck 0 0 3 5 3 8 14 14 8 6 5 7 17 14 20 – – 0 2 4 5 3 8 10
Bud Site 3 11 24 27 22 36 27 25 40 15 21 19 8 10 5 – – 0 – – – – – –
Cell Periphery 0 5 0 0 1 0 0 0 0 1 0 1 3 1 1 – – 0 0 0 0 0 0 0
Cytoplasm 0 8 18 26 26 76 124 135 150 140 119 146 66 93 135 – – 5 0 2 1 2 2 5
Endoplasmic Reticulum 0 0 1 0 0 0 0 1 0 1 0 1 1 0 1 – – 0 0 0 0 0 0 1
Endosome 2 30 56 44 55 40 35 20 8 18 3 18 13 14 16 – – 1 4 19 46 27 28 25
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – – 0 1 2 3 1 5 2
Mitochondria 9 48 43 38 38 111 110 116 150 179 193 205 12 7 2 – – 3 2 2 9 8 13 16
Nucleus 0 1 1 3 6 1 3 2 2 2 3 1 7 2 1 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 0 1 0 0 1 0 0 0 – – 0 0 0 1 0 0 0
Nucleolus 1 5 8 10 6 25 7 7 8 2 6 4 1 1 0 – – 0 0 0 1 0 1 1
Peroxisomes 8 74 195 165 148 165 90 58 68 64 57 65 94 91 60 – – 4 23 63 202 85 109 112
SpindlePole 5 24 182 161 183 278 240 181 159 149 127 138 265 193 177 – – 4 9 25 69 29 25 40
Vac/Vac Membrane 0 1 2 0 1 3 8 3 3 2 2 4 0 4 1 – – 1 0 1 0 0 2 1
Unique Cell Count 20 133 349 307 322 507 492 440 447 445 438 465 402 367 371 16 49 152 366 170 218 256
Labelled Cell Count 31 220 573 532 527 804 718 602 644 638 602 655 519 454 458 19 49 152 366 170 218 256


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 6.9 5.0 5.3 4.8 3.3 3.0 3.2 3.0 2.8 2.8 2.9 5.2 5.1 4.9 4.7 – 6.1 5.5 5.8 5.6
Std Deviation (1e-4) 1.2 1.6 1.5 1.9 1.9 1.1 1.1 1.2 1.1 1.1 1.1 1.0 1.0 1.0 0.9 2.0 – 2.8 1.3 2.4 1.5
Intensity Change (Log2) – – – 0.08 -0.07 -0.61 -0.75 -0.63 -0.75 -0.82 -0.84 -0.79 0.07 0.04 -0.03 -0.07 – 0.28 0.15 0.22 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 2.3 2.2 3.9 – – 0
Bud 1.9 0 0 0 0 0 0 0 0 -3.0 -3.6 -3.0 – – 0
Bud Neck 0 0 0 0 0 0 0 0 0 2.9 2.6 3.5 – – 0
Bud Site 0.9 0 0 0 0 0 0 0 0 -3.3 -2.6 -3.8 – – 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Cytoplasm 1.7 1.5 4.5 7.6 9.0 9.7 9.2 8.1 9.2 4.9 7.5 10.2 – – 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Endosome -0.6 0 0 0 0 0 0 0 0 -6.1 -5.5 -5.2 – – 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Mitochondria 0 0 0 0 0 0 0 0 0 -4.9 -5.5 -6.5 – – 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Nucleolus 0.8 0 0 0 0 0 0 0 0 0 0 0 – – 0
Peroxisomes -0.5 0 0 0 0 0 0 0 0 -9.1 -8.5 -11.1 – – -2.4
SpindlePole 0.1 0 0 0 0 0 0 0 0 3.8 0.1 -1.2 – – -2.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6505 3.5763 3.3851 2.623 2.7793 3.6144 6.1801 6.0543 6.0638 6.1466 5.352 5.9422 5.4895 6.1232 5.6464 5.7329 5.6242 5.9984
Actin 0.09 0.0794 0.0502 0.0111 0.0532 0.0075 0.018 0.0762 0.0562 0.0454 0.0037 0.0119 0.0219 0.0737 0.0595 0.0077 0.0154 0.014
Bud 0.0014 0.0099 0.0072 0.0003 0.002 0.0007 0.0004 0.0077 0.0026 0.001 0.0009 0.0003 0.001 0.0065 0.0058 0.0002 0.0048 0.0005
Bud Neck 0.0171 0.0073 0.0112 0.0266 0.0284 0.0015 0.0007 0.002 0.0141 0.0089 0.0011 0.0228 0.0021 0.0093 0.011 0.0169 0.0002 0.0084
Bud Periphery 0.0013 0.0073 0.0067 0.0003 0.003 0.0019 0.0006 0.0053 0.0018 0.0005 0.0016 0.0006 0.0009 0.0051 0.0034 0.0001 0.0031 0.0006
Bud Site 0.0048 0.0953 0.0553 0.001 0.0032 0.0019 0.0045 0.0935 0.0394 0.0029 0.0058 0.0014 0.0112 0.0878 0.0287 0.0016 0.0018 0.0016
Cell Periphery 0.0007 0.0003 0.0003 0 0.0002 0 0.0001 0.0001 0.0001 0.0004 0.0003 0.0001 0.0001 0.0004 0.0001 0 0 0.0003
Cytoplasm 0.0161 0.0098 0.0055 0.0003 0.001 0.0002 0.0014 0.0007 0.0003 0.0284 0.0008 0.0013 0.0007 0.0045 0.005 0.0002 0.0005 0.0008
Cytoplasmic Foci 0.2701 0.2667 0.2326 0.238 0.295 0.3011 0.3501 0.3042 0.3431 0.2634 0.3139 0.4349 0.4314 0.3641 0.3533 0.4792 0.5697 0.3574
Eisosomes 0.0005 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0003 0.0002 0 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0074 0.0004 0.0007 0 0.0005 0 0.002 0 0 0.0029 0.0005 0.0015 0 0.0002 0.0001 0 0 0.0001
Endosome 0.0519 0.0279 0.0033 0.0032 0.0103 0.0038 0.0079 0.0062 0.0094 0.0043 0.0047 0.019 0.0134 0.0169 0.006 0.0107 0.0032 0.015
Golgi 0.0151 0.0205 0.0104 0.0084 0.0233 0.0132 0.0154 0.0063 0.0099 0.0073 0.0185 0.0149 0.0069 0.0147 0.0067 0.0083 0.006 0.0182
Lipid Particles 0.0639 0.0253 0.0174 0.0619 0.0566 0.0662 0.0551 0.0181 0.036 0.0294 0.0509 0.0408 0.0533 0.0289 0.0265 0.0395 0.0233 0.0393
Mitochondria 0.0166 0.0044 0.0024 0.003 0.0039 0.0078 0.003 0.0011 0.0023 0.0009 0.0086 0.0037 0.001 0.0026 0.0016 0.002 0.0008 0.0015
None 0.0084 0.0011 0.0044 0.0549 0.0003 0.0003 0.0038 0.0003 0.0004 0.0016 0.0057 0.0071 0.0003 0.0006 0.0002 0.0001 0.0001 0.0002
Nuclear Periphery 0.0512 0.0004 0.0002 0 0.0007 0.0001 0.0087 0.0001 0 0.0006 0.0004 0.0049 0.0001 0.0001 0 0 0 0
Nucleolus 0.008 0.0012 0.0003 0.0001 0.001 0.0015 0.0012 0.0007 0.0005 0.0001 0.0031 0.0007 0.0012 0.0046 0.0017 0.0004 0 0.0012
Nucleus 0.0321 0.0008 0.0003 0 0.0002 0.0003 0.001 0.0003 0.0001 0.0008 0.0007 0.0015 0.0003 0.0006 0.0002 0.0001 0 0.0003
Peroxisomes 0.296 0.4004 0.5669 0.5876 0.4643 0.5304 0.5041 0.443 0.4544 0.5975 0.5709 0.389 0.433 0.3534 0.4556 0.4033 0.3587 0.5056
Punctate Nuclear 0.0381 0.0372 0.0243 0.0029 0.052 0.0611 0.0205 0.0338 0.0291 0.0034 0.0064 0.0417 0.0203 0.0248 0.0341 0.0296 0.0122 0.0317
Vacuole 0.0067 0.0041 0.0003 0 0.0005 0.0002 0.0007 0.0002 0.0001 0.0002 0.0008 0.0007 0.0007 0.001 0.0004 0.0001 0 0.0032
Vacuole Periphery 0.0027 0.0004 0 0 0.0002 0.0001 0.0006 0 0 0.0001 0.0003 0.0009 0.0001 0.0002 0.0001 0 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.6001 14.4819 12.7362 17.0609 16.7255 11.3937 16.6763 20.8636 19.2975 16.2048
Translational Efficiency 1.9123 1.5355 1.6805 1.3893 1.1698 2.3957 1.3998 1.1762 1.3149 1.515

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Peroxisomal membrane signal receptor for peroxisomal matrix proteins; receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins; required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions
Localization
Cell Percentages peroxisome (37%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pex5

Pex5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available