ORF | |
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Human Ortholog | |
Description | Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 3 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 1 | – | 1 | 7 | 5 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 16 | 16 |
Bud Neck | 0 | 0 | 2 | 2 | 9 | 1 | 8 | – | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 2 | 1 | 1 | 3 | – | 2 | 3 | 0 | 0 | 2 | 1 | 0 | 2 | 6 | 0 | 0 | 0 | 0 | 1 | 4 | 1 |
Cytoplasm | 154 | 59 | 28 | 247 | 348 | 200 | 403 | – | 231 | 277 | 93 | 105 | 418 | 655 | 685 | 215 | 179 | 67 | 153 | 76 | 56 | 264 | 541 | 465 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 7 | 6 | 3 | 0 | 0 | 0 | 1 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 5 | – | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Mitochondria | 12 | 6 | 0 | 4 | 0 | 58 | 19 | – | 72 | 94 | 85 | 58 | 0 | 0 | 0 | 0 | 3 | 2 | 8 | 1 | 4 | 14 | 27 | 12 |
Nucleus | 1 | 0 | 0 | 1 | 2 | 9 | 5 | – | 7 | 8 | 4 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 5 | 1 | 4 | – | 3 | 5 | 1 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 13 | 8 |
Vac/Vac Membrane | 1 | 0 | 0 | 2 | 2 | 2 | 31 | – | 32 | 20 | 15 | 8 | 6 | 1 | 8 | 4 | 8 | 1 | 5 | 2 | 0 | 4 | 4 | 2 |
Unique Cell Count | 162 | 60 | 29 | 252 | 355 | 228 | 451 | 307 | 355 | 168 | 152 | 424 | 660 | 690 | 216 | 187 | 67 | 178 | 80 | 65 | 306 | 635 | 527 | |
Labelled Cell Count | 170 | 67 | 34 | 260 | 369 | 278 | 480 | 355 | 419 | 207 | 184 | 428 | 662 | 697 | 229 | 204 | 73 | 178 | 80 | 65 | 306 | 635 | 527 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 4.0 | 5.6 | 4.8 | 5.5 | 3.7 | 4.8 | – | 4.8 | 4.6 | 4.6 | 4.5 | 5.0 | 5.2 | 5.6 | 7.4 | 8.2 | 6.5 | 4.5 | 4.6 | 4.9 |
Std Deviation (1e-4) | 1.0 | 0.9 | 1.1 | 1.2 | 1.5 | 1.2 | 1.3 | – | 2.0 | 1.5 | 1.9 | 1.6 | 1.2 | 1.8 | 2.0 | 2.7 | 2.9 | 2.1 | 1.5 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.23 | -0.02 | -0.59 | -0.22 | – | -0.2 | -0.27 | -0.27 | -0.33 | -0.17 | -0.11 | 0.01 | 0.41 | 0.56 | 0.22 | -0.33 | -0.28 | -0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.2327 | -0.1938 | -0.1039 | -0.4211 | 0.6502 | -0.1742 | 0.6656 | 1.6092 | 1.2041 | 1.0254 | 1.0835 | 1.4625 | 3.3827 | 2.9933 | 2.3752 | 2.8605 | 2.4818 | 2.6132 |
Actin | 0.0328 | 0.0001 | 0.0411 | 0.0384 | 0.0136 | 0.0063 | 0.0075 | 0.0002 | 0.0014 | 0.0003 | 0.0009 | 0.0027 | 0.059 | 0.0001 | 0.0082 | 0.0294 | 0.0084 | 0.0009 |
Bud | 0.0007 | 0.0001 | 0.0002 | 0.0015 | 0.0012 | 0.0008 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0015 | 0.0002 | 0.0016 | 0.0001 | 0.0005 | 0.0007 | 0.0002 | 0.0001 |
Bud Neck | 0.0017 | 0.0001 | 0.0005 | 0.0016 | 0.0057 | 0.0007 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.005 | 0.003 | 0 | 0.0017 | 0.0006 | 0.0001 | 0.0003 |
Bud Periphery | 0.0008 | 0.0001 | 0.0003 | 0.0032 | 0.0018 | 0.0018 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0023 | 0.0003 | 0.0021 | 0 | 0.0003 | 0.0006 | 0.0006 | 0 |
Bud Site | 0.0044 | 0.0006 | 0.0011 | 0.0084 | 0.0163 | 0.0004 | 0.0001 | 0.0007 | 0.0002 | 0.0001 | 0.0017 | 0.0001 | 0.0098 | 0.0002 | 0.0067 | 0.0041 | 0.0002 | 0 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.222 | 0.2936 | 0.3325 | 0.2831 | 0.3813 | 0.4228 | 0.5497 | 0.6199 | 0.5236 | 0.6804 | 0.5692 | 0.6887 | 0.2665 | 0.3883 | 0.4158 | 0.4939 | 0.4287 | 0.5749 |
Cytoplasmic Foci | 0.031 | 0.0107 | 0.0105 | 0.0161 | 0.0295 | 0.0184 | 0.0076 | 0.0058 | 0.0066 | 0.0039 | 0.0205 | 0.014 | 0.0127 | 0.0027 | 0.0162 | 0.0122 | 0.0152 | 0.0036 |
Eisosomes | 0.0004 | 0.0001 | 0.0003 | 0.0007 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0053 | 0.002 | 0.0027 | 0.0033 | 0.01 | 0.0021 | 0.0042 | 0.0028 | 0.0021 | 0.002 | 0.0011 | 0.0028 | 0.0034 | 0.001 | 0.003 | 0.0034 | 0.0025 | 0.001 |
Endosome | 0.0396 | 0.0016 | 0.0056 | 0.0237 | 0.0564 | 0.0108 | 0.004 | 0.0024 | 0.0018 | 0.0014 | 0.008 | 0.0063 | 0.0134 | 0.0005 | 0.0113 | 0.01 | 0.0101 | 0.0008 |
Golgi | 0.0079 | 0.0001 | 0.0034 | 0.0058 | 0.0136 | 0.0005 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0033 | 0.0005 | 0.0064 | 0.0001 | 0.0013 | 0.0047 | 0.0044 | 0.0001 |
Lipid Particles | 0.0269 | 0.0002 | 0.0067 | 0.0219 | 0.0145 | 0.0015 | 0.0007 | 0.0003 | 0.0002 | 0 | 0.0007 | 0.0007 | 0.0052 | 0.0001 | 0.0013 | 0.0024 | 0.0223 | 0.0001 |
Mitochondria | 0.0144 | 0.0001 | 0.0019 | 0.0045 | 0.0092 | 0.0006 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.003 | 0.0006 | 0.0019 | 0.0001 | 0.0009 | 0.0013 | 0.0007 | 0.0001 |
None | 0.5129 | 0.687 | 0.5714 | 0.567 | 0.3385 | 0.5197 | 0.4076 | 0.3593 | 0.4582 | 0.3037 | 0.3672 | 0.2668 | 0.5764 | 0.6044 | 0.5157 | 0.4221 | 0.4548 | 0.4155 |
Nuclear Periphery | 0.03 | 0.0004 | 0.005 | 0.0047 | 0.0458 | 0.0025 | 0.0065 | 0.0009 | 0.0008 | 0.001 | 0.0004 | 0.002 | 0.0032 | 0.0003 | 0.0078 | 0.0024 | 0.0037 | 0.0003 |
Nucleolus | 0.0034 | 0.0001 | 0.0002 | 0.001 | 0.0083 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0057 | 0 | 0.0002 | 0.0003 | 0.0003 | 0 |
Nucleus | 0.0306 | 0.001 | 0.0074 | 0.0023 | 0.0209 | 0.0041 | 0.0069 | 0.0024 | 0.0019 | 0.0046 | 0.0028 | 0.0055 | 0.0021 | 0.0009 | 0.0022 | 0.0018 | 0.0008 | 0.0016 |
Peroxisomes | 0.0146 | 0.0007 | 0.0052 | 0.004 | 0.008 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.013 | 0.0003 | 0.0035 | 0 | 0.0027 | 0.0039 | 0.0264 | 0.0001 |
Punctate Nuclear | 0.013 | 0.0008 | 0.0025 | 0.0054 | 0.0142 | 0.0045 | 0.0011 | 0.0027 | 0.0008 | 0.0006 | 0.0024 | 0.0024 | 0.0218 | 0.0003 | 0.002 | 0.005 | 0.0199 | 0.0003 |
Vacuole | 0.0052 | 0.0005 | 0.0009 | 0.0018 | 0.0059 | 0.0009 | 0.0012 | 0.001 | 0.0007 | 0.0008 | 0.001 | 0.0009 | 0.001 | 0.0005 | 0.0014 | 0.0006 | 0.0003 | 0.0002 |
Vacuole Periphery | 0.0021 | 0.0001 | 0.0003 | 0.0011 | 0.0046 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0 | 0.0008 | 0.0002 | 0.0002 | 0 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.9068 | 8.474 | 13.8338 | 17.2883 | 12.2905 | 17.0535 | 19.2782 | 15.6138 | 18.4135 | 18.0422 |
Translational Efficiency | 0.7565 | 1.6941 | 0.9365 | 0.9262 | 1.3087 | 1.219 | 1.2145 | 1.3263 | 1.1742 | 1.1899 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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