ORF | |
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Human Ortholog | |
Description | Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 2 |
Bud | 0 | 2 | 5 | 1 | 2 | 3 | 16 | 6 | 10 | 18 | 11 | 8 | 1 | 1 | 0 | 1 | 0 | 0 | 3 | 2 | 0 | 0 | 3 | 3 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 1 | 3 | 1 | 0 | 0 | 3 | 2 | 4 | 0 | 4 | 0 | 0 | 2 | 38 | 25 | 24 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 623 | 420 | 462 | 444 | 513 | 529 | 681 | 634 | 469 | 472 | 332 | 385 | 508 | 364 | 548 | 467 | 453 | 503 | 611 | 412 | 453 | 296 | 439 | 377 |
Endoplasmic Reticulum | 3 | 4 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 19 | 14 | 19 | 0 | 2 | 1 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 2 | 0 | 0 | 3 |
Golgi | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 4 | 3 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 12 | 0 | 24 | 91 | 29 | 178 | 345 | 226 | 284 | 299 | 205 | 292 | 1 | 1 | 0 | 4 | 12 | 8 | 12 | 4 | 3 | 1 | 0 | 3 |
Nucleus | 2 | 3 | 1 | 0 | 2 | 2 | 7 | 6 | 3 | 1 | 4 | 4 | 1 | 0 | 0 | 0 | 0 | 6 | 0 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 1 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 1 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 3 | 1 | 0 | 1 | 4 | 6 | 5 | 5 | 9 | 9 | 3 | 8 | 0 | 2 | 3 | 17 | 23 | 39 | 0 | 2 | 1 | 2 | 1 | 2 |
Unique Cell Count | 640 | 427 | 470 | 454 | 524 | 599 | 828 | 748 | 596 | 606 | 437 | 543 | 510 | 366 | 551 | 484 | 475 | 534 | 652 | 436 | 475 | 304 | 462 | 403 |
Labelled Cell Count | 646 | 430 | 495 | 541 | 552 | 723 | 1062 | 887 | 779 | 804 | 561 | 716 | 511 | 369 | 553 | 550 | 531 | 605 | 652 | 436 | 475 | 304 | 462 | 403 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 6.7 | 3.9 | 3.8 | 4.5 | 3.5 | 3.3 | 3.6 | 3.0 | 3.1 | 3.2 | 3.0 | 6.7 | 6.7 | 6.8 | 7.9 | 7.9 | 7.6 | 6.6 | 6.7 | 6.9 |
Std Deviation (1e-4) | 0.8 | 0.9 | 0.8 | 1.1 | 1.1 | 0.9 | 1.0 | 1.0 | 1.0 | 0.7 | 1.1 | 0.7 | 1.0 | 1.1 | 1.1 | 1.5 | 1.4 | 1.6 | 1.0 | 1.1 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.19 | -0.19 | -0.26 | -0.12 | -0.41 | -0.35 | -0.32 | -0.41 | 0.77 | 0.76 | 0.78 | 1.01 | 1.0 | 0.95 | 0.75 | 0.77 | 0.82 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.0 | 4.7 | 4.3 |
Cytoplasm | -0.6 | -0.5 | -6.2 | -8.6 | -7.7 | -9.6 | -9.8 | -10.2 | -11.7 | 2.0 | 1.5 | 1.8 | -1.7 | -2.6 | -3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 3.4 | 3.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.9 | -4.1 | -5.4 | -3.9 | -2.1 | -3.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 4.8 | 6.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4172 | 3.0449 | 2.7789 | 2.1745 | 3.0334 | 2.6626 | 1.2641 | 1.4213 | 1.5789 | 1.8203 | 0.9058 | 1.5733 | 3.1346 | 3.9738 | 3.6544 | 3.2077 | 3.7024 | 3.3846 |
Actin | 0.0274 | 0.0022 | 0.0092 | 0.0108 | 0.0483 | 0.0073 | 0.0254 | 0.0011 | 0.0126 | 0.1 | 0.0082 | 0.0095 | 0.0155 | 0.0007 | 0.0124 | 0.0001 | 0.0044 | 0.0016 |
Bud | 0.0009 | 0.0031 | 0.0012 | 0.0096 | 0.0052 | 0.003 | 0.0032 | 0.005 | 0.0013 | 0.0009 | 0.0007 | 0.0008 | 0.0015 | 0.0003 | 0.0032 | 0.0002 | 0.0006 | 0.0021 |
Bud Neck | 0.0019 | 0.0006 | 0.0006 | 0.0021 | 0.0024 | 0.003 | 0.0081 | 0.0005 | 0.0008 | 0.0046 | 0.0094 | 0.0038 | 0.0023 | 0.0005 | 0.0058 | 0.0004 | 0.0009 | 0.0032 |
Bud Periphery | 0.0011 | 0.001 | 0.0007 | 0.0051 | 0.0109 | 0.0017 | 0.0085 | 0.0047 | 0.0026 | 0.001 | 0.0018 | 0.0011 | 0.0021 | 0.0002 | 0.0029 | 0.0001 | 0.001 | 0.0023 |
Bud Site | 0.0027 | 0.0115 | 0.0022 | 0.0033 | 0.0041 | 0.0005 | 0.0091 | 0.0106 | 0.0061 | 0.0112 | 0.0108 | 0.0015 | 0.0145 | 0.0032 | 0.0059 | 0.0001 | 0.0014 | 0.0003 |
Cell Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 |
Cytoplasm | 0.6205 | 0.807 | 0.7178 | 0.6758 | 0.5832 | 0.7622 | 0.5175 | 0.7285 | 0.6225 | 0.6724 | 0.6551 | 0.6332 | 0.5652 | 0.6952 | 0.586 | 0.7468 | 0.6247 | 0.6404 |
Cytoplasmic Foci | 0.0148 | 0.0126 | 0.0129 | 0.0236 | 0.0444 | 0.0223 | 0.0153 | 0.0083 | 0.0178 | 0.0499 | 0.0229 | 0.0186 | 0.0154 | 0.0046 | 0.0101 | 0.0086 | 0.0112 | 0.0102 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0039 | 0.0023 | 0.0031 | 0.0016 | 0.0073 | 0.0024 | 0.0102 | 0.003 | 0.0061 | 0.0058 | 0.0079 | 0.0034 | 0.004 | 0.0017 | 0.0028 | 0.0011 | 0.0017 | 0.0019 |
Endosome | 0.0118 | 0.0032 | 0.0055 | 0.0042 | 0.0602 | 0.0139 | 0.0227 | 0.0027 | 0.0147 | 0.0242 | 0.0222 | 0.0167 | 0.0091 | 0.0012 | 0.0054 | 0.0026 | 0.0034 | 0.003 |
Golgi | 0.002 | 0.0005 | 0.0013 | 0.0034 | 0.0127 | 0.0041 | 0.004 | 0.0002 | 0.0058 | 0.007 | 0.0104 | 0.0046 | 0.0015 | 0.0001 | 0.001 | 0.0001 | 0.0007 | 0.0003 |
Lipid Particles | 0.0012 | 0.0003 | 0.0018 | 0.0171 | 0.0074 | 0.0023 | 0.0043 | 0.0001 | 0.0034 | 0.0089 | 0.0021 | 0.0026 | 0.0007 | 0.0001 | 0.0015 | 0 | 0.0075 | 0.0001 |
Mitochondria | 0.0074 | 0.0004 | 0.0018 | 0.0051 | 0.0069 | 0.0032 | 0.0071 | 0.0003 | 0.0078 | 0.0021 | 0.0078 | 0.0103 | 0.0014 | 0.0002 | 0.0008 | 0.0003 | 0.0016 | 0.0006 |
None | 0.2611 | 0.1383 | 0.2237 | 0.2008 | 0.1195 | 0.1447 | 0.3093 | 0.2207 | 0.2687 | 0.0782 | 0.2106 | 0.2582 | 0.3397 | 0.2777 | 0.3354 | 0.2046 | 0.3051 | 0.3191 |
Nuclear Periphery | 0.0124 | 0.0014 | 0.0023 | 0.0015 | 0.0179 | 0.0109 | 0.025 | 0.0018 | 0.0037 | 0.0056 | 0.0019 | 0.0075 | 0.0073 | 0.0016 | 0.0038 | 0.0018 | 0.0032 | 0.0012 |
Nucleolus | 0.0008 | 0.0003 | 0.0003 | 0.0022 | 0.0018 | 0.0005 | 0.0041 | 0.0003 | 0.0013 | 0.0004 | 0.002 | 0.0011 | 0.0004 | 0.0002 | 0.0008 | 0.0005 | 0.0011 | 0.0001 |
Nucleus | 0.013 | 0.0064 | 0.004 | 0.0131 | 0.0091 | 0.006 | 0.0088 | 0.0067 | 0.0117 | 0.0052 | 0.0097 | 0.0068 | 0.0078 | 0.0081 | 0.0121 | 0.0163 | 0.017 | 0.0083 |
Peroxisomes | 0.005 | 0.0008 | 0.0022 | 0.0099 | 0.0306 | 0.0019 | 0.005 | 0.0002 | 0.0041 | 0.0081 | 0.0062 | 0.0106 | 0.0055 | 0.0001 | 0.0014 | 0.0001 | 0.007 | 0.0004 |
Punctate Nuclear | 0.0083 | 0.0063 | 0.0079 | 0.0088 | 0.0194 | 0.0077 | 0.006 | 0.0036 | 0.0054 | 0.0105 | 0.0072 | 0.0068 | 0.0045 | 0.0036 | 0.0069 | 0.0153 | 0.0062 | 0.0039 |
Vacuole | 0.0023 | 0.0013 | 0.001 | 0.0011 | 0.006 | 0.0013 | 0.0042 | 0.0014 | 0.0024 | 0.0026 | 0.002 | 0.0016 | 0.0012 | 0.0007 | 0.0013 | 0.0009 | 0.0009 | 0.0007 |
Vacuole Periphery | 0.0012 | 0.0002 | 0.0003 | 0.0004 | 0.0024 | 0.0008 | 0.0014 | 0.0002 | 0.0005 | 0.0006 | 0.0005 | 0.0009 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0002 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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