Standard name | |
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Human Ortholog | |
Description | Heterotrimeric nascent polypeptide-associated complex beta3 subunit; complex binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3; BTT1 has a paralog, EGD1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud | 1 | 3 | 2 | 3 | 1 | 9 | – | 20 | 7 | 9 | 11 | 14 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 10 |
Bud Neck | 0 | 0 | 1 | 0 | 3 | 0 | – | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 2 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 1 | 0 | 0 | 0 | – | 0 | 1 | 1 | 2 | 4 | 1 | 1 | 1 | 11 | 10 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 355 | 143 | 102 | 132 | 261 | 356 | – | 554 | 285 | 310 | 227 | 285 | 99 | 131 | 240 | 154 | 215 | 144 | 347 | 136 | 91 | 46 | 122 | 167 |
Endoplasmic Reticulum | 1 | 1 | 1 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 9 | 3 | 8 | 0 | 0 | 0 | 0 | 3 | 2 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | – | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 3 | 3 | 1 | 0 | 0 | 2 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 1 | 1 | 0 | 1 | 5 | 3 |
Mitochondria | 13 | 4 | 9 | 31 | 34 | 190 | – | 362 | 233 | 253 | 204 | 250 | 1 | 4 | 1 | 2 | 11 | 6 | 6 | 0 | 10 | 0 | 7 | 4 |
Nucleus | 0 | 1 | 0 | 1 | 0 | 3 | – | 11 | 2 | 4 | 6 | 2 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | – | 10 | 2 | 3 | 4 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | – | 11 | 5 | 3 | 1 | 5 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 1 | 2 | 1 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Vac/Vac Membrane | 1 | 1 | 1 | 0 | 1 | 0 | – | 10 | 11 | 8 | 7 | 5 | 0 | 0 | 1 | 13 | 22 | 14 | 6 | 6 | 2 | 2 | 10 | 6 |
Unique Cell Count | 363 | 146 | 110 | 139 | 272 | 443 | 766 | 400 | 444 | 348 | 437 | 101 | 133 | 248 | 166 | 238 | 163 | 377 | 149 | 108 | 55 | 164 | 216 | |
Labelled Cell Count | 374 | 154 | 117 | 168 | 303 | 559 | 989 | 549 | 596 | 464 | 577 | 103 | 140 | 250 | 192 | 268 | 186 | 377 | 149 | 108 | 55 | 164 | 216 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.7 | 3.2 | 3.8 | 4.1 | 3.2 | – | 3.9 | 3.4 | 3.3 | 3.4 | 3.3 | 5.0 | 5.3 | 5.3 | 8.4 | 8.2 | 8.0 | 5.0 | 5.9 | 5.7 |
Std Deviation (1e-4) | 0.6 | 1.0 | 0.5 | 1.3 | 1.8 | 1.0 | – | 2.6 | 2.7 | 1.9 | 3.1 | 2.6 | 1.0 | 1.4 | 1.8 | 1.8 | 1.8 | 1.4 | 0.8 | 2.3 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.23 | 0.36 | -0.02 | – | 0.27 | 0.08 | 0.04 | 0.08 | 0.04 | 0.64 | 0.72 | 0.72 | 1.38 | 1.35 | 1.31 | 0.63 | 0.87 | 0.84 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | 1.3 | -3.1 | – | -4.6 | -4.7 | -4.9 | -5.6 | -5.7 | 1.8 | 2.3 | 1.7 | 0 | -0.7 | -1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -1.8 | 0 | 0 | -1.3 | -1.6 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.9 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1202 | 0.6823 | 0.4742 | 0.5641 | 0.2164 | 0.7186 | 0.153 | 0.3663 | 0.3521 | -0.0649 | 0.3115 | 0.2748 | 1.5068 | 2.3513 | 2.3116 | 2.1958 | 1.8244 | 2.2371 |
Actin | 0.0165 | 0.0011 | 0.0084 | 0.0115 | 0.0251 | 0.0044 | 0.0245 | 0.0023 | 0.0165 | 0.0496 | 0.0406 | 0.0022 | 0.0094 | 0.0002 | 0.0052 | 0.0002 | 0.0005 | 0.0001 |
Bud | 0.0004 | 0.0001 | 0.0005 | 0.0014 | 0.0003 | 0.0003 | 0.0036 | 0.0002 | 0.0021 | 0.0091 | 0.0008 | 0.0015 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0019 | 0.0001 | 0.0017 | 0.0357 | 0.0012 | 0.0006 | 0.0039 | 0.0001 | 0.0006 | 0.0011 | 0.0005 | 0.0098 | 0.0001 | 0.0001 | 0.0056 | 0.0001 | 0.0001 | 0.0004 |
Bud Periphery | 0.0005 | 0.0001 | 0.0006 | 0.0014 | 0.0004 | 0.0004 | 0.003 | 0.0002 | 0.0024 | 0.0073 | 0.0012 | 0.0011 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0008 | 0.0013 | 0.0029 | 0.0054 | 0.0015 | 0.0003 | 0.0118 | 0.0015 | 0.0162 | 0.0135 | 0.0016 | 0.0001 | 0.0003 | 0.0012 | 0.0007 | 0.0001 | 0.0001 | 0 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.373 | 0.4595 | 0.488 | 0.4823 | 0.4641 | 0.6178 | 0.4864 | 0.6338 | 0.5717 | 0.5477 | 0.4952 | 0.6208 | 0.3985 | 0.6262 | 0.5979 | 0.4646 | 0.4781 | 0.6832 |
Cytoplasmic Foci | 0.0373 | 0.0179 | 0.0215 | 0.044 | 0.0377 | 0.0211 | 0.0367 | 0.0106 | 0.0369 | 0.025 | 0.0356 | 0.0265 | 0.0095 | 0.0082 | 0.008 | 0.0066 | 0.009 | 0.0129 |
Eisosomes | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0098 | 0.0074 | 0.0035 | 0.0027 | 0.0042 | 0.0048 | 0.0085 | 0.003 | 0.0029 | 0.0028 | 0.0046 | 0.0045 | 0.0055 | 0.0034 | 0.0025 | 0.0018 | 0.0043 | 0.0028 |
Endosome | 0.0268 | 0.0068 | 0.0133 | 0.0231 | 0.0468 | 0.0183 | 0.0324 | 0.0059 | 0.0232 | 0.0351 | 0.0749 | 0.0133 | 0.0041 | 0.0035 | 0.0085 | 0.003 | 0.0049 | 0.0097 |
Golgi | 0.0081 | 0.0006 | 0.0018 | 0.0102 | 0.0101 | 0.0018 | 0.015 | 0.0003 | 0.0111 | 0.0146 | 0.0387 | 0.0021 | 0.0005 | 0.0002 | 0.001 | 0.0001 | 0.0003 | 0.0002 |
Lipid Particles | 0.0082 | 0.0014 | 0.0034 | 0.0043 | 0.0315 | 0.0017 | 0.014 | 0.0002 | 0.0113 | 0.0069 | 0.0214 | 0.0127 | 0.0012 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0.0001 |
Mitochondria | 0.0041 | 0.0003 | 0.0018 | 0.0048 | 0.0204 | 0.0032 | 0.0112 | 0.0002 | 0.015 | 0.0283 | 0.0115 | 0.0008 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0003 |
None | 0.4924 | 0.4978 | 0.4341 | 0.346 | 0.3205 | 0.3138 | 0.3031 | 0.3363 | 0.2485 | 0.2248 | 0.1562 | 0.2979 | 0.5642 | 0.3523 | 0.3617 | 0.5197 | 0.4981 | 0.2872 |
Nuclear Periphery | 0.0044 | 0.0014 | 0.002 | 0.0022 | 0.0054 | 0.0026 | 0.004 | 0.0007 | 0.0026 | 0.002 | 0.0066 | 0.0018 | 0.0009 | 0.0007 | 0.001 | 0.0008 | 0.0008 | 0.0004 |
Nucleolus | 0.0003 | 0.0001 | 0.0003 | 0.0009 | 0.0014 | 0.0005 | 0.0007 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0022 | 0.001 | 0.0017 | 0.0049 | 0.0032 | 0.0024 | 0.0028 | 0.0012 | 0.0019 | 0.0026 | 0.0024 | 0.0014 | 0.0008 | 0.0013 | 0.0013 | 0.0011 | 0.0011 | 0.001 |
Peroxisomes | 0.0045 | 0.0002 | 0.0094 | 0.0063 | 0.015 | 0.0012 | 0.025 | 0.0002 | 0.0269 | 0.0151 | 0.0984 | 0.0009 | 0.0024 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0044 | 0.0009 | 0.0018 | 0.0081 | 0.0068 | 0.0025 | 0.0069 | 0.0006 | 0.006 | 0.0018 | 0.005 | 0.0012 | 0.0007 | 0.0006 | 0.0016 | 0.0004 | 0.0005 | 0.0003 |
Vacuole | 0.003 | 0.0015 | 0.0024 | 0.0033 | 0.0031 | 0.0014 | 0.004 | 0.0022 | 0.0027 | 0.0083 | 0.0027 | 0.0011 | 0.0013 | 0.001 | 0.0012 | 0.0007 | 0.0011 | 0.0011 |
Vacuole Periphery | 0.0008 | 0.0002 | 0.0005 | 0.0007 | 0.001 | 0.0006 | 0.0015 | 0.0002 | 0.0007 | 0.0037 | 0.0017 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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