Standard name
Human Ortholog
Description Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.93 0.95 0.99 0.98 0.93 0.92 – 0.83 0.83 0.66 0.79 0.97 0.95 0.97 – 0.93 0.91 0.59 0.85 0.72 0.73 0.78 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.08 0.07 0.07 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.15 0.1 0.05 0 0 0.18 0.08 – 0.29 0.22 0.45 0.29 0 0 0 – 0 0 0.12 0.05 0.15 0.11 0.09 0.09
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 – 0.05 0.06 0.06 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 – 1 0 0 0 1 3 5 8
Bud 1 1 0 0 0 0 2 – 3 1 8 4 0 1 0 – 0 0 1 0 0 3 5 14
Bud Neck 0 0 0 3 4 2 11 – 5 7 1 8 0 0 1 – 0 0 0 0 0 2 2 3
Bud Site 0 0 0 0 0 0 0 – 1 0 1 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 2 1 1 0 – 2 1 0 0 0 0 0 – 0 1 0 0 0 4 3 4
Cytoplasm 57 63 18 209 371 183 407 – 206 249 140 248 364 513 544 – 164 137 57 83 33 267 365 447
Endoplasmic Reticulum 0 2 0 0 0 0 0 – 0 0 1 0 1 0 0 – 14 11 6 0 0 2 0 2
Endosome 0 0 0 0 0 0 2 – 0 1 0 1 2 11 3 – 1 1 4 0 0 4 3 3
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 – 1 1 1 0 1 0 0 1
Mitochondria 10 7 1 2 5 35 35 – 72 65 96 92 5 6 2 – 2 5 11 4 6 39 43 49
Nucleus 2 0 0 1 0 2 5 – 6 10 5 2 0 3 2 – 1 1 1 1 0 1 0 5
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 – 0 1 0 0 0 0 3 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 1 1 – 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 3 0 9 – 2 5 5 7 0 0 1 – 0 0 3 0 0 7 10 14
Vac/Vac Membrane 1 1 0 0 3 0 6 – 0 1 1 1 8 18 26 – 8 9 5 4 0 11 12 14
Unique Cell Count 65 68 19 212 378 196 442 247 299 212 312 374 539 563 177 151 98 98 47 365 469 582
Labelled Cell Count 72 74 19 217 388 223 478 297 340 258 366 380 554 580 192 167 98 98 47 365 469 582


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.6 3.9 4.0 5.0 3.4 3.9 – 3.4 3.7 3.3 3.7 4.2 4.3 4.6 4.8 5.4 5.0 3.9 4.1 4.1
Std Deviation (1e-4) 0.4 0.5 0.8 0.9 1.6 1.3 1.4 – 1.4 1.5 1.4 1.7 1.0 1.2 1.6 1.4 1.2 1.0 1.4 1.6 1.3
Intensity Change (Log2) – – – 0.05 0.36 -0.21 0.02 – -0.21 -0.06 -0.22 -0.07 0.11 0.15 0.24 0.29 0.46 0.37 -0.0 0.07 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1993 -1.3289 -1.2657 -1.5422 -1.7555 -1.3175 -0.1148 -0.4461 -0.3654 -1.1485 -0.8254 -0.4895 0.753 0.7039 0.8356 0.7596 0.5194 0.8198
Actin 0.0376 0.0003 0.0199 0.002 0.0001 0.002 0.0349 0.0006 0.0136 0.0024 0.0006 0.0004 0.0331 0.0001 0.0012 0.0185 0.0001 0.0001
Bud 0.0003 0.0002 0.0003 0.0004 0 0.0001 0.0011 0.0004 0.0037 0.0001 0.0012 0.0001 0.0002 0 0.0005 0.0001 0 0
Bud Neck 0.0008 0.0003 0.0001 0.0003 0.0001 0.0013 0.0027 0.0002 0.0005 0.0005 0.0002 0.0009 0.0076 0.0001 0.0002 0.0009 0.0003 0.0003
Bud Periphery 0.0005 0.0002 0.0002 0.0009 0 0.0004 0.0017 0.0013 0.0019 0.0001 0.0012 0.0001 0.0002 0 0.0005 0.0001 0 0
Bud Site 0.0011 0.0022 0.0006 0.0005 0 0.0001 0.0094 0.0008 0.0025 0.0001 0.0015 0.0001 0.0029 0.0003 0.0014 0.0007 0.0001 0
Cell Periphery 0.0002 0.0001 0 0.0001 0 0 0.0006 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0002 0 0 0
Cytoplasm 0.0445 0.0472 0.0345 0.0478 0.0517 0.0663 0.0799 0.1639 0.1455 0.1978 0.1221 0.2647 0.0495 0.0977 0.1115 0.1059 0.0496 0.1536
Cytoplasmic Foci 0.0137 0.0063 0.0063 0.004 0.0227 0.0159 0.0341 0.0101 0.0175 0.0232 0.0073 0.0134 0.009 0.0018 0.0039 0.0241 0.0087 0.0131
Eisosomes 0.0009 0.0001 0.0004 0.0003 0 0.0003 0.0008 0.0001 0.0002 0.0001 0 0 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0038 0.0008 0.001 0.0011 0.0005 0.0008 0.0035 0.0014 0.0028 0.0043 0.0014 0.0024 0.0012 0.0012 0.0016 0.0027 0.0006 0.0016
Endosome 0.0177 0.0006 0.0017 0.0009 0.0014 0.0033 0.0237 0.0009 0.0097 0.0134 0.034 0.0095 0.0028 0.0005 0.0032 0.0063 0.0007 0.0015
Golgi 0.005 0.0001 0.001 0.0002 0.0001 0.0005 0.0124 0.0001 0.0068 0.0009 0.0033 0.0051 0.0019 0 0.0015 0.0033 0 0
Lipid Particles 0.0079 0.0004 0.002 0.0005 0.0009 0.0043 0.0263 0.0006 0.0113 0.0062 0.0032 0.006 0.0023 0.0001 0.0004 0.0078 0.0001 0.0002
Mitochondria 0.003 0.0003 0.0007 0.0009 0.0002 0.0006 0.0072 0.0006 0.0215 0.0021 0.059 0.0009 0.0004 0.0002 0.0067 0.0007 0.0002 0.0002
None 0.8264 0.9117 0.8901 0.8803 0.9148 0.8062 0.6951 0.7724 0.6996 0.6535 0.6973 0.5883 0.8427 0.872 0.8233 0.7363 0.85 0.8081
Nuclear Periphery 0.0104 0.0012 0.0071 0.0048 0.0008 0.0075 0.0073 0.0011 0.0042 0.0115 0.0029 0.0041 0.0021 0.0009 0.0023 0.0025 0.0012 0.001
Nucleolus 0.0009 0.0016 0.0009 0.001 0.0002 0.0021 0.0065 0.0004 0.0012 0.0006 0.001 0.001 0.0015 0.0004 0.0004 0.0007 0.0009 0.0001
Nucleus 0.0075 0.0137 0.0153 0.0431 0.0008 0.0687 0.0252 0.0378 0.028 0.0641 0.0538 0.0835 0.028 0.022 0.0326 0.0321 0.0804 0.016
Peroxisomes 0.0068 0.0003 0.0073 0.0004 0.0006 0.0013 0.015 0.0009 0.0115 0.0034 0.0014 0.0005 0.0054 0 0.0001 0.007 0.0001 0.0003
Punctate Nuclear 0.0089 0.0121 0.0099 0.0097 0.0046 0.0172 0.008 0.0055 0.0138 0.0132 0.004 0.0176 0.0081 0.0022 0.0071 0.0495 0.0066 0.0033
Vacuole 0.0013 0.0004 0.0004 0.0005 0.0003 0.0006 0.0036 0.0008 0.0031 0.0018 0.0035 0.0012 0.0005 0.0003 0.0008 0.0005 0.0003 0.0003
Vacuole Periphery 0.0011 0 0.0002 0.0002 0.0001 0.0003 0.0011 0.0001 0.0009 0.0005 0.0009 0.0003 0.0001 0 0.0003 0.0002 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –
Standard name
Human Ortholog
Description Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (17%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Met32

Met32


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met32-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available