Standard name
Human Ortholog
Description Exo-1,3-beta-glucanase; involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.79 0.84 0.93 0.95 0.93 0.92 0.89 0.91 0.9 0.89 0.9 0.99 0.98 0.98 0.45 0.45 0.44 0.11 0.14 0.09 0.21 0.25 0.24
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0.11 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.33 0.21 0.22 0.06 0.05 0.05 0.13 0.14 0.2 0.2 0.11 0.14 0 0 0 0.68 0.67 0.65 0.75 0.75 0.85 0.71 0.67 0.67
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 3 3 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 0 2 2 5 2 11 4 4 6 12 0 0 0 0 0 0 0 2 0 0 1 2
Bud Neck 0 0 2 0 0 0 0 1 0 0 0 0 0 0 3 0 0 0 1 2 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 2 2 1 1 1 2 3 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 65 186 126 208 351 361 493 749 394 430 388 610 142 256 335 140 124 135 13 36 14 9 33 51
Endoplasmic Reticulum 0 8 3 6 10 13 2 2 0 1 3 1 0 1 0 5 8 14 1 8 1 0 0 1
Endosome 0 1 0 2 0 2 4 8 1 1 2 8 0 5 1 7 6 3 2 5 1 0 2 4
Golgi 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 2 0 0 1 1 0 0 0 0
Mitochondria 11 4 0 0 0 1 0 3 4 3 16 13 0 0 1 2 0 2 4 7 2 1 1 4
Nucleus 1 7 4 3 0 3 3 18 6 9 8 9 0 0 0 34 26 34 0 0 0 0 2 2
Nuclear Periphery 1 0 0 0 0 1 1 1 1 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 31 50 33 14 19 18 72 121 86 95 48 98 6 6 3 212 187 198 89 203 138 32 90 145
Unique Cell Count 93 235 150 224 370 388 537 842 435 478 436 679 144 261 341 311 278 304 119 271 162 45 135 218
Labelled Cell Count 109 257 169 235 387 410 578 918 497 547 475 754 148 268 345 402 352 386 119 271 162 45 135 218


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.3 6.3 6.0 7.0 6.6 6.7 6.9 5.8 5.7 5.2 5.5 5.9 5.8 5.5 7.5 7.9 7.6 – 6.1 6.6
Std Deviation (1e-4) 0.7 0.9 0.9 1.0 1.3 1.2 1.4 1.4 1.2 1.1 1.1 3.6 1.4 1.3 1.8 1.4 1.5 1.5 – 1.8 2.0
Intensity Change (Log2) – – – -0.08 0.15 0.06 0.07 0.12 -0.12 -0.14 -0.29 -0.21 -0.1 -0.13 -0.21 0.24 0.32 0.26 – -0.04 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.7 4.1 3.2 2.8 1.7 2.2 2.0 1.6 2.1 4.4 5.4 6.1 -7.9 -7.9 -8.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 3.0 2.6 3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.5 -5.8 -6.2 -2.6 -2.4 -0.6 -0.6 -3.4 -2.3 -4.5 -6.6 -8.3 9.3 8.9 8.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2071 0.6737 0.7342 0.5742 0.1198 0.5167 0.4782 0.1207 0.6305 0.0558 -0.2092 0.0216 0.1983 -0.479 -0.0277 0.2007 0.2491 -0.2649
Actin 0.0318 0.0011 0.0007 0.0002 0.0002 0.0032 0.004 0.0164 0.0192 0.0004 0.0967 0.0456 0.0655 0.0046 0.018 0.0529 0.0013 0.0084
Bud 0.0027 0.0025 0.0026 0.0015 0.0009 0.003 0.0004 0.0004 0.0008 0.0013 0.0003 0.0004 0.0014 0.002 0.0017 0.0004 0.0003 0.0011
Bud Neck 0.0062 0.0029 0.0024 0.0004 0.0006 0.0137 0.0011 0.0006 0.0009 0.0002 0.0002 0.0237 0.0034 0.0057 0.001 0.0009 0.0004 0.0035
Bud Periphery 0.003 0.0053 0.0028 0.0036 0.0011 0.0024 0.0005 0.0005 0.0013 0.0024 0.0005 0.0009 0.0026 0.0015 0.0012 0.0003 0.0002 0.0015
Bud Site 0.0098 0.011 0.0061 0.0005 0.0007 0.0021 0.0064 0.0024 0.0054 0.0007 0.0009 0.002 0.0365 0.0057 0.0052 0.0016 0.0006 0.0033
Cell Periphery 0.0009 0.0008 0.0006 0.0002 0.0004 0.0004 0.001 0.0015 0.0005 0.0005 0.0003 0.0019 0.0015 0.0008 0.0004 0.0002 0.0001 0.0006
Cytoplasm 0.1386 0.2063 0.1667 0.1597 0.185 0.2092 0.2249 0.1705 0.1437 0.1463 0.0969 0.2796 0.1255 0.2479 0.2739 0.1803 0.2279 0.2581
Cytoplasmic Foci 0.0598 0.039 0.0628 0.0869 0.0477 0.0569 0.0579 0.0316 0.0455 0.0447 0.0166 0.0289 0.0554 0.0392 0.0567 0.088 0.0941 0.034
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0003 0 0.0003 0.0003 0.0021 0.0001 0.0004 0.0002 0 0.0001
Endoplasmic Reticulum 0.0062 0.0063 0.0069 0.003 0.0065 0.0045 0.0218 0.0097 0.012 0.0102 0.0053 0.0094 0.0111 0.0065 0.0086 0.0054 0.0043 0.0154
Endosome 0.0932 0.0766 0.099 0.1917 0.1679 0.0892 0.1094 0.0884 0.1329 0.2259 0.0539 0.1261 0.1426 0.0867 0.1748 0.2169 0.2938 0.1729
Golgi 0.0078 0.0014 0.0029 0.0021 0.0015 0.0016 0.0091 0.0021 0.0101 0.0039 0.0036 0.0068 0.0169 0.0041 0.0175 0.0097 0.0183 0.0085
Lipid Particles 0.0083 0.0054 0.0113 0.0123 0.0194 0.0065 0.0335 0.0099 0.0111 0.0039 0.0058 0.0074 0.0465 0.0058 0.0144 0.0087 0.0168 0.0044
Mitochondria 0.0045 0.0027 0.0051 0.0032 0.0024 0.0049 0.0024 0.0014 0.0311 0.005 0.0032 0.0034 0.0137 0.0022 0.0066 0.0019 0.0034 0.0071
None 0.4999 0.471 0.5024 0.4101 0.4208 0.4385 0.4155 0.4976 0.4856 0.3425 0.6375 0.2629 0.2608 0.4321 0.2959 0.1589 0.1869 0.1943
Nuclear Periphery 0.0048 0.0081 0.0053 0.0037 0.0065 0.0059 0.009 0.004 0.008 0.0074 0.0021 0.0051 0.0148 0.0041 0.0079 0.0042 0.0057 0.0174
Nucleolus 0.0025 0.0042 0.0044 0.0014 0.0015 0.0022 0.0064 0.0011 0.0009 0.0007 0.0002 0.0021 0.0025 0.0012 0.0009 0.0005 0.0005 0.001
Nucleus 0.0063 0.0241 0.0108 0.0045 0.0055 0.0151 0.0063 0.0029 0.0032 0.0039 0.0011 0.0277 0.0096 0.0077 0.0038 0.0025 0.0016 0.0105
Peroxisomes 0.0038 0.0007 0.0029 0.0019 0.0012 0.0025 0.0052 0.0006 0.0075 0.0005 0.0563 0.0046 0.0186 0.0007 0.0059 0.0331 0.0116 0.0043
Punctate Nuclear 0.0038 0.0072 0.0085 0.0037 0.0021 0.0087 0.0109 0.0007 0.0028 0.001 0.0005 0.0028 0.0139 0.0029 0.0089 0.0042 0.0199 0.0022
Vacuole 0.0981 0.1154 0.0889 0.0982 0.1164 0.119 0.0692 0.1499 0.0719 0.1744 0.0149 0.1478 0.143 0.1326 0.0868 0.207 0.0928 0.2294
Vacuole Periphery 0.0076 0.0077 0.0067 0.0112 0.0118 0.0102 0.0049 0.0075 0.0051 0.024 0.0027 0.0105 0.012 0.0059 0.0096 0.0221 0.0194 0.0221

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.8907 33.872 26.0012 24.7388 38.9628 35.2581 37.6432 30.0894 27.8763 39.5598
Translational Efficiency 0.9097 0.9335 0.7167 0.6585 0.6461 0.886 0.7749 0.639 0.6127 0.7118

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Exo-1,3-beta-glucanase; involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
Localization
Cell Percentages vacuole (28%), cytoplasm (7%), mixed (49%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Exg2

Exg2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Exg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available