Standard name | |
---|---|
Human Ortholog | |
Description | Peroxisomal membrane E3 ubiquitin ligase; required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 24 | 13 | 3 | 6 | 6 | 2 | 3 | – | 3 | 8 | 11 | 20 | 25 | 34 | 3 | 6 | 5 | 15 | 14 | 4 | 3 | 28 | 33 |
Bud | 5 | 10 | 47 | 48 | 135 | 165 | 186 | 165 | – | 180 | 114 | 247 | 8 | 23 | 26 | 10 | 10 | 8 | 2 | 1 | 1 | 0 | 2 | 1 |
Bud Neck | 2 | 1 | 9 | 5 | 12 | 6 | 12 | 8 | – | 7 | 2 | 8 | 5 | 8 | 3 | 2 | 5 | 1 | 4 | 3 | 0 | 3 | 2 | 6 |
Bud Site | 5 | 4 | 18 | 27 | 66 | 99 | 64 | 41 | – | 54 | 40 | 82 | 3 | 8 | 7 | 4 | 7 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 3 | 1 | 4 | 2 | 3 | – | 1 | 4 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 2 | 7 | 18 | 48 | 53 | 34 | 50 | – | 51 | 22 | 49 | 8 | 6 | 9 | 5 | 7 | 7 | 1 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 4 | 1 | 1 | 2 | 0 | 3 | 0 | – | 0 | 1 | 0 | 5 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 25 | 10 | 3 | 9 | 17 | 16 | 13 | 38 | – | 12 | 1 | 7 | 10 | 33 | 71 | 12 | 13 | 22 | 91 | 23 | 40 | 20 | 25 | 40 |
Golgi | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 7 | 6 | 5 | 3 | 5 | 15 | 1 | 1 | 0 | 1 | 4 |
Mitochondria | 108 | 83 | 125 | 144 | 374 | 541 | 538 | 299 | – | 408 | 360 | 611 | 14 | 46 | 32 | 24 | 9 | 10 | 18 | 13 | 8 | 6 | 25 | 29 |
Nucleus | 7 | 0 | 1 | 2 | 4 | 8 | 10 | 14 | – | 2 | 3 | 8 | 2 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 3 | 3 | 0 | 1 | 1 | – | 1 | 3 | 0 | 1 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 19 | 0 | 0 | 4 | 10 | 7 | 14 | 14 | – | 18 | 5 | 14 | 0 | 3 | 2 | 3 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 90 | 54 | 85 | 169 | 247 | 263 | 289 | 236 | – | 297 | 127 | 349 | 29 | 207 | 233 | 64 | 57 | 67 | 165 | 87 | 169 | 67 | 163 | 169 |
SpindlePole | 24 | 6 | 71 | 127 | 184 | 269 | 367 | 395 | – | 371 | 150 | 396 | 46 | 122 | 151 | 45 | 32 | 53 | 16 | 8 | 14 | 3 | 6 | 10 |
Vac/Vac Membrane | 1 | 1 | 0 | 0 | 2 | 2 | 5 | 6 | – | 4 | 2 | 1 | 2 | 1 | 3 | 3 | 2 | 3 | 2 | 0 | 0 | 1 | 3 | 1 |
Unique Cell Count | 214 | 125 | 226 | 326 | 669 | 873 | 953 | 819 | 888 | 546 | 1105 | 114 | 336 | 372 | 120 | 112 | 133 | 337 | 155 | 248 | 111 | 263 | 307 | |
Labelled Cell Count | 296 | 204 | 381 | 563 | 1111 | 1439 | 1540 | 1273 | 1409 | 842 | 1785 | 155 | 492 | 580 | 183 | 154 | 191 | 337 | 155 | 248 | 111 | 263 | 307 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 4.7 | 3.3 | 3.4 | 3.4 | 3.4 | 3.1 | 3.6 | – | 3.1 | 3.0 | 3.2 | 5.6 | 5.5 | 5.6 | 6.1 | 6.0 | 5.9 | 5.9 | 5.7 | 5.6 |
Std Deviation (1e-4) | 8.9 | 0.8 | 0.6 | 1.0 | 1.2 | 1.0 | 1.1 | 1.2 | – | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 0.7 | 1.8 | 1.5 | 1.6 | 1.6 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.02 | 0.04 | -0.1 | 0.09 | – | -0.09 | -0.14 | -0.08 | 0.73 | 0.71 | 0.74 | 0.86 | 0.85 | 0.82 | 0.81 | 0.78 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0716 | -0.2064 | -0.2627 | -0.0866 | -0.7442 | -0.166 | 0.4111 | 0.6989 | 0.7785 | 0.6253 | 0.243 | 0.6793 | -1.7392 | -1.3475 | -1.4261 | -0.9556 | -1.6658 | -1.544 |
Actin | 0.0462 | 0.0073 | 0.0362 | 0.0046 | 0.0156 | 0.0062 | 0.0256 | 0.0272 | 0.0258 | 0.0084 | 0.042 | 0.028 | 0.029 | 0.0647 | 0.0204 | 0.0114 | 0.0046 | 0.0182 |
Bud | 0.0004 | 0.0002 | 0.0045 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0019 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0015 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0042 | 0.0051 | 0.0092 | 0.0017 | 0.0002 | 0.0033 | 0.0011 | 0.0021 | 0.0063 | 0.0028 | 0.0008 | 0.0027 | 0.0013 | 0.0077 | 0.0208 | 0.0215 | 0.0001 | 0.0029 |
Bud Periphery | 0.0005 | 0.0002 | 0.0036 | 0.0003 | 0.0008 | 0.0001 | 0.0004 | 0.005 | 0.0024 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0055 | 0.0002 | 0 | 0.0001 |
Bud Site | 0.0036 | 0.0169 | 0.0084 | 0.0004 | 0.0017 | 0.0003 | 0.0044 | 0.0157 | 0.0123 | 0.0023 | 0.0007 | 0.0003 | 0.0014 | 0.0311 | 0.0078 | 0.0038 | 0.0001 | 0.0003 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0 | 0 |
Cytoplasm | 0.0064 | 0.0226 | 0.0022 | 0.0015 | 0.0003 | 0.0012 | 0.0045 | 0.0158 | 0.0044 | 0.0002 | 0.0019 | 0.0045 | 0.0013 | 0.0107 | 0.004 | 0.002 | 0.0006 | 0.0069 |
Cytoplasmic Foci | 0.1384 | 0.2183 | 0.1212 | 0.0893 | 0.0672 | 0.1838 | 0.1766 | 0.2085 | 0.1244 | 0.0712 | 0.1246 | 0.1616 | 0.2126 | 0.2529 | 0.199 | 0.2454 | 0.1966 | 0.2224 |
Eisosomes | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0003 | 0.0015 | 0.0007 | 0.001 | 0.0003 | 0.0003 | 0.0027 | 0.0002 | 0.0004 | 0.001 | 0.0002 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0009 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0008 | 0.0006 | 0 | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0006 | 0.0021 | 0 | 0.0005 |
Endosome | 0.0286 | 0.0384 | 0.0067 | 0.0122 | 0.0068 | 0.0085 | 0.0205 | 0.0201 | 0.0162 | 0.0213 | 0.0381 | 0.0098 | 0.0242 | 0.0207 | 0.0204 | 0.0175 | 0.0027 | 0.0127 |
Golgi | 0.0238 | 0.019 | 0.0077 | 0.0146 | 0.0088 | 0.0082 | 0.0133 | 0.0105 | 0.0113 | 0.0151 | 0.0125 | 0.0057 | 0.0144 | 0.0175 | 0.0158 | 0.0066 | 0.0063 | 0.0167 |
Lipid Particles | 0.084 | 0.0384 | 0.029 | 0.0669 | 0.0444 | 0.0681 | 0.0685 | 0.0157 | 0.0179 | 0.0359 | 0.0539 | 0.0454 | 0.1309 | 0.0466 | 0.0648 | 0.0936 | 0.0369 | 0.0892 |
Mitochondria | 0.0153 | 0.0055 | 0.0191 | 0.0762 | 0.0469 | 0.0081 | 0.0078 | 0.0202 | 0.0392 | 0.0624 | 0.0115 | 0.0116 | 0.0114 | 0.0062 | 0.0202 | 0.0017 | 0.002 | 0.0027 |
None | 0.0205 | 0.0661 | 0.0435 | 0.05 | 0.0019 | 0.0327 | 0.0255 | 0.12 | 0.0583 | 0.0015 | 0.062 | 0.0498 | 0.0164 | 0.0374 | 0.0354 | 0.0078 | 0.0025 | 0.0032 |
Nuclear Periphery | 0.0015 | 0.0003 | 0.0035 | 0.0002 | 0 | 0.0001 | 0.0007 | 0.0004 | 0.0008 | 0 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.0039 | 0.0155 | 0 | 0.0104 |
Nucleolus | 0.0143 | 0.0007 | 0.0003 | 0.0009 | 0.0001 | 0.0003 | 0.0006 | 0.0009 | 0.0007 | 0.0002 | 0.0006 | 0.0007 | 0.0007 | 0.0008 | 0.0033 | 0.0029 | 0.0001 | 0.0003 |
Nucleus | 0.0029 | 0.0009 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0006 | 0.0007 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.002 | 0.0023 | 0 | 0.0009 |
Peroxisomes | 0.5973 | 0.5559 | 0.6971 | 0.6774 | 0.8032 | 0.6703 | 0.6367 | 0.5241 | 0.6724 | 0.7766 | 0.6424 | 0.6599 | 0.5477 | 0.4956 | 0.5645 | 0.5517 | 0.7398 | 0.5969 |
Punctate Nuclear | 0.0088 | 0.0025 | 0.0058 | 0.0019 | 0.0006 | 0.0079 | 0.0089 | 0.0076 | 0.0019 | 0.0004 | 0.0065 | 0.0143 | 0.005 | 0.0033 | 0.0067 | 0.0117 | 0.0077 | 0.0148 |
Vacuole | 0.0011 | 0.0009 | 0.0003 | 0.0008 | 0.0002 | 0.0003 | 0.0013 | 0.0013 | 0.0019 | 0.0006 | 0.0007 | 0.0011 | 0.0014 | 0.0022 | 0.0015 | 0.0008 | 0 | 0.0002 |
Vacuole Periphery | 0.0006 | 0.0002 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0006 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0007 | 0.0007 | 0 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.7935 | 9.7276 | 8.6619 | 11.4871 | 9.1706 | 5.4378 | 13.278 | 17.9235 | 14.3804 | 14.5788 |
Translational Efficiency | 0.4313 | 0.5129 | 0.5424 | 0.3835 | 0.5836 | 1.3007 | 0.4716 | 0.4371 | 0.5969 | 0.4652 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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