Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial tryptophanyl-tRNA synthetase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 18 | 1 | 9 | 0 | 1 | 2 | 0 | 0 | 4 | 0 | 7 | 8 |
Bud | 7 | – | 9 | 13 | 16 | 12 | 37 | 20 | 15 | 24 | 17 | 24 | 15 | 13 | 15 | 7 | 7 | 8 | 0 | 1 | 1 | 1 | 4 | 5 |
Bud Neck | 0 | – | 2 | 2 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 4 | 5 |
Bud Site | 2 | – | 4 | 3 | 5 | 8 | 13 | 16 | 9 | 10 | 5 | 11 | 4 | 0 | 3 | 4 | 2 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 7 | 7 | 4 | 7 | 6 | 3 | 5 | 6 | 1 | 5 | 9 | 13 | 10 | 6 | 9 | 6 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 4 | – | 9 | 8 | 11 | 8 | 14 | 5 | 7 | 3 | 3 | 3 | 57 | 96 | 136 | 36 | 15 | 18 | 0 | 0 | 0 | 2 | 10 | 11 |
Endoplasmic Reticulum | 1 | – | 0 | 1 | 2 | 2 | 0 | 0 | 1 | 2 | 3 | 0 | 3 | 4 | 3 | 13 | 11 | 5 | 0 | 0 | 1 | 0 | 1 | 2 |
Endosome | 0 | – | 24 | 13 | 37 | 4 | 5 | 31 | 2 | 2 | 0 | 0 | 74 | 165 | 165 | 45 | 49 | 47 | 12 | 4 | 20 | 4 | 41 | 48 |
Golgi | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 5 | 3 | 4 | 7 | 1 | 0 | 0 | 3 | 0 | 6 | 6 |
Mitochondria | 162 | – | 170 | 294 | 430 | 478 | 710 | 681 | 547 | 670 | 483 | 574 | 225 | 126 | 149 | 31 | 13 | 9 | 139 | 19 | 170 | 65 | 274 | 269 |
Nucleus | 1 | – | 5 | 1 | 4 | 13 | 27 | 14 | 6 | 9 | 3 | 8 | 2 | 2 | 3 | 3 | 2 | 4 | 0 | 1 | 0 | 0 | 4 | 3 |
Nuclear Periphery | 1 | – | 1 | 4 | 1 | 20 | 27 | 23 | 49 | 37 | 43 | 34 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 1 |
Nucleolus | 4 | – | 0 | 3 | 9 | 11 | 52 | 44 | 28 | 20 | 16 | 21 | 2 | 2 | 2 | 4 | 2 | 4 | 1 | 0 | 0 | 0 | 4 | 3 |
Peroxisomes | 2 | – | 44 | 48 | 53 | 12 | 32 | 42 | 3 | 2 | 2 | 7 | 58 | 102 | 115 | 58 | 61 | 42 | 3 | 0 | 18 | 3 | 15 | 19 |
SpindlePole | 8 | – | 28 | 27 | 28 | 18 | 31 | 55 | 14 | 12 | 7 | 23 | 81 | 160 | 141 | 58 | 44 | 45 | 6 | 0 | 10 | 6 | 31 | 30 |
Vac/Vac Membrane | 19 | – | 15 | 13 | 50 | 36 | 28 | 29 | 36 | 14 | 16 | 40 | 45 | 87 | 85 | 31 | 18 | 23 | 4 | 2 | 5 | 3 | 38 | 40 |
Unique Cell Count | 170 | 231 | 339 | 500 | 514 | 772 | 767 | 583 | 692 | 503 | 593 | 469 | 584 | 640 | 208 | 178 | 148 | 176 | 33 | 247 | 93 | 454 | 465 | |
Labelled Cell Count | 211 | 318 | 438 | 650 | 629 | 985 | 963 | 723 | 813 | 600 | 751 | 600 | 779 | 840 | 300 | 244 | 218 | 176 | 33 | 247 | 93 | 454 | 465 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | – | 4.7 | 4.5 | 4.7 | 4.3 | 4.2 | 4.4 | 4.3 | 4.2 | 3.9 | 4.2 | 5.9 | 5.8 | 5.7 | 6.6 | 6.8 | 6.3 | 5.5 | 5.9 | 6.3 |
Std Deviation (1e-4) | 0.5 | – | 1.4 | 1.3 | 1.2 | 1.1 | 1.2 | 1.1 | 1.0 | 0.9 | 0.8 | 1.0 | 1.0 | 1.0 | 1.0 | 1.9 | 1.7 | 1.4 | 1.7 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.03 | -0.11 | -0.15 | -0.07 | -0.12 | -0.15 | -0.24 | -0.15 | 0.34 | 0.33 | 0.3 | 0.51 | 0.54 | 0.43 | 0.23 | 0.35 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.5 | -1.3 | -1.2 | -0.3 | 0 | 0.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.9 | -0.7 | 0 | -0.1 | 1.0 | 0.5 |
Cytoplasm | -1.1 | -1.3 | -2.0 | -1.9 | 0 | 0 | 0 | 0 | 0 | 3.5 | 4.8 | 6.1 | 4.6 | 1.9 | 3.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 5.4 | 4.9 | 3.2 | 4.5 | 5.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.4 | -13.9 | -13.6 | -12.3 | -13.4 | -12.8 |
Nucleus | 0 | 0 | 0.3 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.4 | -0.5 | -0.4 | 2.2 | 3.5 | 2.1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 4.7 | 3.3 | 4.2 | 3.3 | 4.4 |
Vacuole | -1.4 | 1.5 | 0.3 | -1.9 | -1.8 | -0.2 | -3.4 | -2.1 | 0.1 | 1.4 | 3.3 | 2.8 | 2.9 | 1.3 | 2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7404 | 1.727 | 1.4403 | 1.086 | 1.1247 | 1.418 | 0.5103 | 0.8762 | 0.6369 | 0.6841 | 0.5863 | 0.5063 | -0.1638 | 0.403 | 0.0843 | 0.0302 | -0.6439 | -0.0352 |
Actin | 0.0373 | 0.0059 | 0.0233 | 0.0003 | 0.004 | 0.0134 | 0.0546 | 0.0204 | 0.033 | 0.0941 | 0.0016 | 0.017 | 0.0259 | 0.0223 | 0.0106 | 0.0329 | 0.0008 | 0.0165 |
Bud | 0.0006 | 0.001 | 0.0005 | 0.0004 | 0.0043 | 0.0002 | 0.0008 | 0.0038 | 0.0022 | 0.0179 | 0.0002 | 0.0013 | 0.0006 | 0.0051 | 0.0005 | 0.0006 | 0.0006 | 0.0009 |
Bud Neck | 0.0007 | 0.0005 | 0.0006 | 0.0002 | 0.0022 | 0.0005 | 0.0011 | 0.001 | 0.0029 | 0.0006 | 0.0001 | 0.0081 | 0.0011 | 0.0005 | 0.0012 | 0.0008 | 0.0002 | 0.001 |
Bud Periphery | 0.001 | 0.0013 | 0.0008 | 0.0007 | 0.0054 | 0.0006 | 0.0012 | 0.0071 | 0.0036 | 0.0102 | 0.0003 | 0.0058 | 0.0012 | 0.0062 | 0.0009 | 0.001 | 0.0022 | 0.0018 |
Bud Site | 0.0019 | 0.008 | 0.0071 | 0.0006 | 0.0071 | 0.0004 | 0.0051 | 0.0178 | 0.0101 | 0.0087 | 0.0004 | 0.0007 | 0.0026 | 0.0101 | 0.0023 | 0.0026 | 0.0008 | 0.0008 |
Cell Periphery | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0003 | 0.0011 | 0.0006 | 0.0012 | 0.0001 | 0.0002 | 0.0007 | 0.001 | 0.0004 | 0.0001 | 0.0002 | 0.0008 |
Cytoplasm | 0.0185 | 0.0029 | 0.0052 | 0.0032 | 0.0088 | 0.0057 | 0.0105 | 0.0187 | 0.0045 | 0.0036 | 0.0033 | 0.0041 | 0.0056 | 0.0057 | 0.002 | 0.0005 | 0.0005 | 0.0025 |
Cytoplasmic Foci | 0.0599 | 0.04 | 0.0204 | 0.0082 | 0.0785 | 0.012 | 0.0412 | 0.0422 | 0.0304 | 0.0169 | 0.0108 | 0.0296 | 0.0246 | 0.0283 | 0.028 | 0.0238 | 0.015 | 0.0134 |
Eisosomes | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0016 | 0.0007 | 0.0006 | 0.0005 | 0.0009 | 0.0002 | 0.0003 | 0.0004 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0003 |
Endoplasmic Reticulum | 0.0033 | 0.001 | 0.0009 | 0.0002 | 0.0001 | 0.0008 | 0.0021 | 0.0026 | 0.0006 | 0.0019 | 0.001 | 0.0006 | 0.0023 | 0.0027 | 0.0005 | 0.0013 | 0.0003 | 0.0018 |
Endosome | 0.0727 | 0.0704 | 0.0356 | 0.0329 | 0.0245 | 0.0313 | 0.0744 | 0.0987 | 0.0547 | 0.029 | 0.0494 | 0.0383 | 0.1002 | 0.0909 | 0.0737 | 0.0373 | 0.0511 | 0.0473 |
Golgi | 0.0621 | 0.0674 | 0.032 | 0.0181 | 0.0176 | 0.06 | 0.0707 | 0.0913 | 0.0571 | 0.0475 | 0.0565 | 0.0305 | 0.055 | 0.08 | 0.0774 | 0.0469 | 0.0354 | 0.0614 |
Lipid Particles | 0.0472 | 0.0209 | 0.0127 | 0.0096 | 0.0099 | 0.0157 | 0.0336 | 0.0151 | 0.0134 | 0.0357 | 0.0222 | 0.0224 | 0.0479 | 0.0169 | 0.034 | 0.044 | 0.0088 | 0.0159 |
Mitochondria | 0.5627 | 0.7094 | 0.8118 | 0.9136 | 0.7913 | 0.7935 | 0.6179 | 0.622 | 0.7574 | 0.6882 | 0.8069 | 0.7968 | 0.6483 | 0.6883 | 0.7167 | 0.7629 | 0.8663 | 0.8029 |
None | 0.0198 | 0.0115 | 0.0009 | 0.0004 | 0.0029 | 0.0081 | 0.0141 | 0.0068 | 0.002 | 0.0061 | 0.0136 | 0.0003 | 0.0052 | 0.0035 | 0.0004 | 0.0011 | 0.0001 | 0.0006 |
Nuclear Periphery | 0.0145 | 0.0004 | 0.0067 | 0 | 0.0001 | 0.0023 | 0.0034 | 0.0012 | 0.0003 | 0.0004 | 0.0047 | 0.0005 | 0.0045 | 0.001 | 0.0002 | 0.0052 | 0.0001 | 0.0008 |
Nucleolus | 0.001 | 0.0011 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0.0007 | 0.0003 | 0.0019 | 0.002 | 0.0001 | 0.001 | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.0003 |
Nucleus | 0.0113 | 0.0004 | 0.0014 | 0.0001 | 0.0003 | 0.0002 | 0.001 | 0.0009 | 0.0004 | 0.002 | 0.0038 | 0.0003 | 0.0009 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0003 |
Peroxisomes | 0.0685 | 0.0459 | 0.0317 | 0.0058 | 0.0381 | 0.0441 | 0.0558 | 0.0297 | 0.0189 | 0.0281 | 0.0073 | 0.0387 | 0.0458 | 0.0219 | 0.0404 | 0.032 | 0.0115 | 0.0164 |
Punctate Nuclear | 0.0101 | 0.0008 | 0.0035 | 0 | 0.0005 | 0.0045 | 0.0023 | 0.0036 | 0.0009 | 0.0006 | 0.0095 | 0.0002 | 0.003 | 0.0006 | 0.0001 | 0.0026 | 0.0001 | 0.0013 |
Vacuole | 0.0032 | 0.0068 | 0.0019 | 0.0027 | 0.0016 | 0.0013 | 0.0041 | 0.0104 | 0.0038 | 0.0021 | 0.0031 | 0.0018 | 0.0137 | 0.0061 | 0.0049 | 0.0011 | 0.0026 | 0.0055 |
Vacuole Periphery | 0.0027 | 0.0037 | 0.0023 | 0.0025 | 0.0016 | 0.0036 | 0.0045 | 0.0044 | 0.0022 | 0.0024 | 0.0028 | 0.0025 | 0.0096 | 0.0073 | 0.0048 | 0.002 | 0.0028 | 0.0073 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.3901 | 24.2268 | 13.483 | 12.0001 | 9.4119 | 10.641 | 14.8724 | 20.6662 | 17.8765 | 17.9075 |
Translational Efficiency | 0.6824 | 0.4452 | 0.8302 | 1.0943 | 1.0609 | 1.0033 | 1.0833 | 0.846 | 0.9161 | 0.6136 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|