Standard name
Human Ortholog
Description Protein of unknown function; expression is regulated by phosphate levels
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.92 0.95 0.95 0.9 0.9 0.86 0.81 0.8 0.76 0.74 0.91 0.97 0.99 0.97 – 0.94 0.72 0.65 0.5 0.57 0.59 0.53
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 – 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.13 0 0.22 0.19 0.21 0.35 0.31 0.44 0.42 0 0 0 0 – 0.11 0 0.06 0.12 0.05 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0.09 0 0 0 – 0.06 0.2 0.2 0.27 0.31 0.25 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 – 0 5 2 2 0 4 3
Bud 0 2 1 2 1 2 11 19 14 17 15 11 2 1 0 0 – 0 2 7 1 0 6 10
Bud Neck 0 1 2 1 4 1 6 6 3 7 1 6 0 1 0 0 – 0 3 1 1 1 3 3
Bud Site 0 0 1 0 0 2 1 3 0 2 0 3 0 0 0 0 – 0 – – – – – –
Cell Periphery 2 7 1 2 0 1 1 1 1 0 4 2 0 0 0 1 – 0 0 1 1 0 1 1
Cytoplasm 490 451 161 256 369 418 583 604 368 412 294 379 245 495 520 60 – 17 381 312 83 88 254 226
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 1 1 1 0 0 1 0 7 – 0 0 2 1 0 2 2
Endosome 0 1 0 0 4 1 1 3 0 0 0 0 8 2 0 0 – 0 5 13 2 2 5 9
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 – 0 0 2 0 1 3 3
Mitochondria 13 3 14 34 10 103 123 149 157 159 168 217 0 1 1 1 – 2 9 29 19 8 25 19
Nucleus 0 1 0 0 0 0 1 1 0 0 1 0 0 0 0 2 – 0 0 0 0 0 1 2
Nuclear Periphery 0 3 0 0 0 1 1 1 1 3 0 0 0 0 0 1 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 2 1 0 1 4 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 – 0 0 0 0 0 0 1
SpindlePole 0 0 0 1 1 1 9 11 5 11 10 12 0 0 1 0 – 0 4 2 1 1 4 8
Vac/Vac Membrane 15 19 8 4 20 3 7 4 8 5 1 8 24 20 4 1 – 1 102 96 45 48 106 130
Unique Cell Count 510 467 175 269 389 463 648 704 455 517 385 514 270 511 524 62 18 527 481 168 157 429 431
Labelled Cell Count 521 489 188 302 410 533 745 806 558 619 496 640 279 523 527 73 20 527 481 168 157 429 431


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.2 3.4 3.1 3.9 3.0 2.9 3.1 2.6 2.8 2.7 2.6 4.4 4.3 4.2 5.1 4.7 5.0 3.9 4.2 4.5
Std Deviation (1e-4) 0.5 1.0 2.3 1.1 1.5 1.5 1.0 0.9 1.0 0.6 1.0 0.8 0.9 1.0 0.8 0.9 0.5 1.6 1.0 1.0 1.3
Intensity Change (Log2) – – – -0.1 0.21 -0.17 -0.22 -0.14 -0.38 -0.26 -0.31 -0.38 0.37 0.36 0.31 0.59 0.47 0.56 0.22 0.33 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1722 -0.7427 -0.7673 -0.8881 -0.8155 -0.8024 -1.2654 -1.62 -1.428 -2.0116 -2.009 -1.5501 -0.4557 -0.0962 0.0475 0.8858 -1.2205 -0.0074
Actin 0.029 0.0002 0.0003 0.0002 0.0166 0.0002 0.0827 0.0004 0.0151 0.0001 0.0563 0.0046 0.0277 0.0001 0.0154 0.0416 0 0.001
Bud 0.0001 0.0002 0.0009 0 0.0001 0 0.001 0.0001 0.0047 0 0.0006 0.0013 0.0004 0 0.0002 0.0003 0 0.0008
Bud Neck 0.0004 0.0001 0.0001 0.0001 0.0018 0.0001 0.0105 0 0.001 0 0.0001 0.0015 0.001 0.0001 0.0009 0.0307 0 0.0017
Bud Periphery 0.0002 0.0003 0.0004 0.0002 0.0001 0 0.0016 0.0001 0.0176 0 0.0006 0.0092 0.0006 0 0.0003 0.0006 0 0.0005
Bud Site 0.0008 0.0022 0.0056 0 0.0001 0 0.0163 0.0014 0.0114 0 0.0007 0.0015 0.0022 0.0003 0.0019 0.001 0 0.0001
Cell Periphery 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0005 0.0001 0.0009 0 0 0.0003 0.0001 0.0001 0.0001 0.0003 0 0.0001
Cytoplasm 0.0289 0.0453 0.0303 0.0233 0.0602 0.045 0.0213 0.0355 0.042 0.0284 0.0104 0.032 0.0163 0.0208 0.0293 0.0469 0.0266 0.0606
Cytoplasmic Foci 0.0087 0.0043 0.0024 0.0023 0.0018 0.0029 0.0436 0.0037 0.0172 0.0008 0.003 0.0112 0.0173 0.0009 0.0041 0.0037 0.0006 0.0099
Eisosomes 0.0006 0.0002 0.0003 0.0002 0.0003 0 0.0011 0.0003 0.0004 0.0001 0.0002 0.0003 0.0003 0.0001 0.0005 0.0005 0 0.0001
Endoplasmic Reticulum 0.0005 0.0004 0.0003 0.0003 0.0076 0.0002 0.0018 0.0006 0.0011 0.0001 0.0002 0.0006 0.001 0.0002 0.0006 0.0013 0.0001 0.0002
Endosome 0.0034 0.0011 0.0002 0.0005 0.0043 0.0002 0.0151 0.0004 0.0146 0.0001 0.0021 0.0036 0.0051 0.0001 0.0027 0.0042 0 0.0003
Golgi 0.0021 0.0001 0 0.0001 0.0017 0 0.0141 0.0001 0.0061 0 0.0158 0.0034 0.0013 0 0.0014 0.0036 0 0.0001
Lipid Particles 0.0052 0.0003 0.0003 0.0002 0.0006 0.0001 0.0218 0.0004 0.0162 0.0001 0.0003 0.0005 0.0071 0.0001 0.0016 0.0022 0 0.0001
Mitochondria 0.0007 0.0003 0.0001 0.0016 0.0006 0 0.0107 0.0001 0.0196 0.0001 0.0035 0.022 0.0005 0.0001 0.0007 0.0007 0 0.0006
None 0.9094 0.9433 0.9567 0.9689 0.8955 0.9501 0.7185 0.9556 0.8185 0.9697 0.9047 0.9038 0.9035 0.9767 0.9262 0.8577 0.9725 0.918
Nuclear Periphery 0.0011 0.0001 0.0002 0.0003 0.0054 0.0001 0.0017 0.0002 0.0007 0.0001 0.0001 0.0003 0.0019 0.0001 0.0034 0.001 0 0.0002
Nucleolus 0.0003 0.0001 0.0002 0.0002 0.0002 0 0.0008 0.0001 0.0004 0 0 0.0002 0.0006 0.0001 0.001 0.0003 0 0.0011
Nucleus 0.0003 0.0002 0.0003 0.0004 0.0015 0.0002 0.0005 0.0002 0.0008 0.0001 0.0001 0.0005 0.0012 0.0001 0.0012 0.0005 0 0.002
Peroxisomes 0.0045 0.0004 0.0001 0.0002 0.0001 0.0001 0.0283 0.0001 0.0075 0 0.0009 0.0004 0.0073 0 0.0016 0.0008 0 0.0003
Punctate Nuclear 0.0034 0.0003 0.0005 0.0004 0.001 0.0005 0.0066 0.0003 0.0013 0.0001 0.0002 0.0019 0.0041 0.0001 0.0063 0.0011 0 0.0023
Vacuole 0.0002 0.0004 0.0003 0.0003 0.0003 0.0001 0.001 0.0003 0.0021 0.0001 0.0001 0.0004 0.0005 0.0001 0.0003 0.0006 0 0.0002
Vacuole Periphery 0.0001 0.0001 0 0.0001 0.0002 0 0.0005 0 0.0008 0 0 0.0004 0.0002 0 0.0002 0.0002 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.2543 6.2627 20.9124 11.8013 4.5416 3.5009 21.3712 17.0953 31.7903 21.4165
Translational Efficiency 2.2506 2.7508 0.8969 1.453 2.933 4.619 1.7025 2.6462 1.27 1.885

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; expression is regulated by phosphate levels
Localization
Cell Percentages vacuole (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Phm6

Phm6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Phm6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available