Standard name
Human Ortholog
Description Subunit of the HDA1 histone deacetylase complex; possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance; relocalizes to the cytosol in response to hypoxia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.07 0.05 0.07 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0.07 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0 0 0.07 0.07 0.17 0.13 0.19 0.2 0 0 0.05 0 0 0 0 0 0
Nucleus 0.93 0.95 0.93 0.91 0.93 0.88 0.89 0.82 0.81 0.77 0.77 0.82 0.87 0.85 0.9 0.91 0.85 0.73 0.52 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.06 0.16 0.19 0.31 0.26 0 0 0 0.05 0 0 0.1 0.11 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0.05 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 1 1 0 2 1 2 1 0 1 0 1 1 0 0
Bud Neck 0 0 0 0 0 0 0 2 0 4 2 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3 9 9 7 10 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 5 12 17 14 15 11 8 8 11 6 14 8 6 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 0 3 1 2 0 1 0 0 0 0
Endosome 0 0 1 0 2 0 1 0 1 0 0 2 1 3 1 1 1 0 0 1
Golgi 0 0 1 1 0 0 2 0 0 0 0 0 0 1 1 1 1 0 0 0
Mitochondria 25 3 1 6 1 15 24 35 31 49 53 6 5 5 1 1 3 0 0 0
Nucleus 156 250 171 214 250 189 298 169 196 195 199 135 138 94 145 254 165 7 7 13
Nuclear Periphery 1 0 0 0 2 1 2 2 3 0 4 1 1 0 0 0 0 0 0 0
Nucleolus 6 4 0 1 12 8 19 34 46 79 67 4 1 2 7 5 5 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 3 2 3 0 4 0 4 1 2 0 1 1 1 0 1 0
Vac/Vac Membrane 2 7 2 5 2 1 1 1 3 0 1 6 9 7 2 10 7 0 0 5
Unique Cell Count 167 262 184 236 270 216 336 207 241 252 259 165 159 111 162 281 195 10 15 25
Labelled Cell Count 191 269 190 245 286 232 366 261 301 347 347 174 167 121 162 281 195 10 15 25


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 7.7 7.2 7.2 7.7 6.3 6.5 6.3 6.2 5.7 5.8 8.0 7.9 7.4
Std Deviation (1e-4) 0.8 1.2 2.0 2.0 2.4 1.9 1.8 6.8 1.9 1.8 1.7 2.2 2.1 2.2
Intensity Change (Log2) -0.0 0.1 -0.2 -0.15 -0.21 -0.21 -0.34 -0.32 0.14 0.12 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.6 0.2 -1.7 -1.2 -1.5 -1.0 -2.2 0.7 -0.6 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.3 3.4 5.6 4.8 6.1 6.3 0 0 0
Nucleus -0.8 -0.1 -1.8 -1.6 -3.3 -3.5 -4.4 -4.5 -3.2 -1.9 -2.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 3.3 5.8 6.3 8.4 7.5 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 2.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.137 1.4164 1.1657 0.9117 0.9102 0.9329 1.7695 1.8745 1.6912 1.5418 1.5812 1.6108 2.4217 2.347 2.0624 2.156 2.1583 2.0285
Actin 0.0043 0 0.006 0.0228 0.0018 0.0012 0.0283 0 0.0098 0 0.0093 0.0029 0.0006 0 0.0014 0.0037 0.0011 0.0005
Bud 0.0008 0 0.0001 0.0006 0.001 0 0.0006 0 0 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0001 0
Bud Neck 0.0007 0 0.0002 0.0029 0.0013 0.0006 0.0018 0 0.0001 0 0.0009 0.0006 0.001 0 0.0003 0.0002 0.0005 0.0008
Bud Periphery 0.0019 0 0.0002 0.0008 0.0022 0.0001 0.0011 0 0 0 0.0002 0.0002 0.0006 0.0001 0.0002 0 0.0003 0.0001
Bud Site 0.002 0 0.0004 0.0016 0.0052 0.0001 0.0039 0 0.0001 0 0.0004 0.0002 0.0014 0.0002 0.0014 0.0001 0.0006 0.0001
Cell Periphery 0.0005 0 0.0001 0.0002 0.0005 0 0.0003 0 0 0 0 0.0001 0.0002 0 0.0001 0 0.0001 0
Cytoplasm 0.0017 0.0092 0.0004 0.0029 0.0011 0.0003 0.0014 0 0.0001 0 0.0003 0.0001 0.0019 0.0021 0.0032 0.0001 0.0003 0.0002
Cytoplasmic Foci 0.0056 0 0.0057 0.031 0.0115 0.0011 0.023 0 0.0001 0 0.0241 0.0015 0.0114 0.0005 0.0123 0.0061 0.0011 0.0002
Eisosomes 0.0002 0 0.001 0.0001 0.0001 0 0.0003 0 0 0 0.0001 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0019 0.0002 0.0003 0.0034 0.0005 0.0009 0.0022 0 0 0 0.0004 0.0003 0.0016 0.0009 0.0014 0 0.0011 0.0005
Endosome 0.0157 0.0001 0.0041 0.0368 0.0599 0.0064 0.0071 0 0.0001 0 0.0049 0.0025 0.0228 0.0031 0.0207 0.012 0.0038 0.002
Golgi 0.0035 0 0.0036 0.0236 0.0097 0.0058 0.0048 0 0.0004 0 0.0044 0.0042 0.0035 0.0001 0.0033 0.0251 0.0031 0.0005
Lipid Particles 0.0161 0 0.0114 0.0216 0.0409 0.0126 0.0088 0 0.0001 0 0.0335 0.003 0.0066 0.0001 0.0045 0.001 0.0167 0.0004
Mitochondria 0.0121 0.0002 0.0244 0.0209 0.0505 0.0031 0.0039 0.0001 0.0003 0 0.0022 0.0026 0.0047 0.0002 0.0016 0.0071 0.0039 0.0072
None 0.0009 0.0026 0.005 0.003 0.0004 0.0002 0.0024 0 0 0 0.0009 0.0003 0.0012 0.0001 0.0038 0 0.0003 0
Nuclear Periphery 0.0063 0.0021 0.0008 0.0249 0.0054 0.0223 0.0043 0.0005 0.0003 0.0001 0.009 0.0022 0.0157 0.0017 0.0043 0.0001 0.0129 0.0017
Nucleolus 0.0152 0.0094 0.0104 0.0058 0.0201 0.0336 0.0155 0.0097 0.0101 0.0112 0.0592 0.022 0.0126 0.0095 0.0095 0.0024 0.0834 0.0449
Nucleus 0.8947 0.9753 0.9165 0.7592 0.7405 0.9085 0.8433 0.9894 0.9768 0.9885 0.8061 0.9524 0.8953 0.9778 0.9243 0.9022 0.8632 0.9375
Peroxisomes 0.008 0 0.0079 0.0176 0.03 0.0009 0.023 0 0.0001 0 0.0316 0.002 0.0023 0 0.0023 0.0394 0.0005 0.0001
Punctate Nuclear 0.0008 0.0005 0.0009 0.0162 0.001 0.0008 0.0219 0.0003 0.0014 0.0001 0.0119 0.0022 0.0049 0.0009 0.0016 0.0001 0.0035 0.0029
Vacuole 0.005 0.0001 0.0003 0.002 0.011 0.0004 0.0014 0 0 0 0.0001 0.0003 0.0084 0.0017 0.0032 0.0001 0.0016 0.0002
Vacuole Periphery 0.002 0.0001 0.0003 0.002 0.0053 0.0009 0.0006 0 0 0 0.0004 0.0004 0.0033 0.0004 0.0007 0.0002 0.0018 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.2631 11.2033 8.3133 13.242 10.2439 12.5256 15.1445 10.1384 14.0547 15.9041
Translational Efficiency 1.1186 0.8517 1.0104 0.5888 0.9738 0.9636 0.7162 1.1106 0.8273 0.8304

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the HDA1 histone deacetylase complex; possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Hda2

Hda2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hda2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available