Standard name
Human Ortholog
Description Component of the SSU and 90S preribosomes; involved in pre-18S rRNA processing; binds to U3 snoRNA and Mpp10p; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.07 0 0 0.06 0.06 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0.08 0 0.1 0.1 0.11 0 0.1 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0.09 0.09 0.14 0.25 0.27 0.22 0.33 0.31 0.2 0.34 0 0 0 0.11 0.1 0.06 0 0 0 0 0 0
Nucleus 0.17 0.26 0.16 0.07 0.05 0.1 0.1 0.09 0.08 0.08 0.08 0.06 0.07 0.05 0.05 0.17 0.12 0.15 0.1 0.16 0.12 0 0.06 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.9 0.92 0.88 0.91 0.91 0.85 0.82 0.82 0.8 0.83 0.82 0.83 0.85 0.84 0.81 0.87 0.8 0.82 0.83 0.77 0.71 0.81 0.74 0.72
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.25 0.24 0.19 0.27 0.27 0.19 0.23 0.22 0.23 0 0.1 0.1 0 0 0 0 0 0.1 0.09 0.12 0.1
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2 3 2 4 3 7 0 2 0 1 0 0 0 1 1 0 1 2 6 4
Bud Neck 1 2 3 5 5 2 2 8 1 2 0 2 10 6 0 0 0 0 0 0 0 0 1 4
Bud Site 0 0 2 5 2 8 31 8 13 21 3 10 3 0 0 0 3 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3 7 5 4 6 8 13 4 3 4 4 49 51 61 7 10 11 0 1 0 1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Endosome 1 3 1 2 5 4 6 7 5 5 1 1 4 12 21 1 0 0 2 4 7 1 2 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 4 2
Mitochondria 20 8 27 26 48 88 114 110 108 116 10 41 11 10 8 20 10 8 0 0 2 2 4 0
Nucleus 50 60 48 19 18 33 42 44 25 28 4 7 35 27 25 31 12 19 30 36 37 9 20 19
Nuclear Periphery 1 0 2 2 1 2 2 3 1 3 0 2 1 0 0 1 1 0 0 0 0 0 0 3
Nucleolus 263 211 267 253 324 294 346 406 262 309 40 100 417 446 440 155 82 102 262 180 223 218 266 246
Peroxisomes 0 1 2 1 0 2 3 3 1 3 0 0 2 16 16 2 0 2 0 0 1 0 0 1
SpindlePole 1 3 31 68 84 65 114 133 62 84 11 27 19 55 54 3 2 1 13 3 30 23 43 32
Vac/Vac Membrane 1 0 3 2 1 2 2 3 3 2 0 0 1 0 3 3 2 1 0 5 5 4 4 11
Unique Cell Count 292 230 302 277 355 346 424 498 328 373 49 120 492 532 546 178 103 124 316 234 314 269 362 340
Labelled Cell Count 338 291 393 388 494 509 672 742 488 583 73 196 552 625 628 223 122 146 316 234 314 269 362 340


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.9 16.5 10.8 9.8 8.6 7.4 5.8 6.3 6.3 5.8 5.7 5.4 15.2 14.8 13.3 19.9 22.5 24.3 14.9 14.3 15.6
Std Deviation (1e-4) 7.5 5.1 4.3 5.6 3.5 3.5 2.3 2.3 1.9 1.7 1.6 1.5 7.3 7.3 7.2 9.1 13.2 12.9 5.5 5.0 5.9
Intensity Change (Log2) – – – -0.13 -0.33 -0.54 -0.9 -0.77 -0.77 -0.88 -0.91 -0.99 0.5 0.46 0.31 0.88 1.06 1.17 0.47 0.41 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 4.2 0 2.7 3.5 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -1.2 -0.5 -0.4 0.3 -1.1 0 0 0 4.1 4.0 4.5 1.0 0 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 3.1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 1.8 5.5 6.0 4.8 7.3 7.0 2.4 6.4 -4.3 -4.8 -5.2 0.8 0.2 -0.8
Nucleus -3.4 -4.6 -2.4 -2.4 -3.0 -3.2 -3.4 -1.4 -2.8 -3.9 -5.2 -5.6 0.4 -1.0 -0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.2 1.2 -1.3 -2.5 -2.6 -2.9 -2.0 -1.3 -1.4 -1.4 -1.8 -2.9 -0.4 -2.2 -1.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 2.2 2.2 0 0 0
SpindlePole 4.6 4.5 3.0 5.5 5.6 3.1 4.2 2.4 3.3 -3.6 0 -0.2 -3.5 -2.7 -3.4
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0 0 0 0.0009 0.0323 0 0.0464 0 0.0036 0.0008 0.0022 0 – – – – – –
Bud 0.0001 0.0001 0 0.0002 0.0005 0 0.0005 0 0.0002 0.0001 0.0002 0 – – – – – –
Bud Neck 0.0002 0.0002 0.0001 0.001 0.0016 0.0002 0.0007 0.0001 0.0004 0.0004 0.0028 0.0001 – – – – – –
Bud Periphery 0.0001 0 0 0.0003 0.0009 0 0.0009 0 0.0002 0.0001 0.0004 0.0001 – – – – – –
Bud Site 0.0004 0.0003 0.0008 0.0009 0.001 0 0.0017 0.0001 0.003 0.0006 0.0015 0.0002 – – – – – –
Cell Periphery 0.0005 0.0001 0.0002 0.0004 0.0001 0.0001 0.0003 0.0001 0.0003 0.0002 0.0005 0.0001 – – – – – –
Cytoplasm 0.0001 0 0 0.0001 0.0254 0 0.0086 0 0.0013 0 0.0001 0 – – – – – –
Cytoplasmic Foci 0.0007 0.0001 0.0003 0.0054 0.0691 0.0001 0.0222 0.0004 0.0177 0.0087 0.0069 0.0002 – – – – – –
Eisosomes 0 0 0 0.0001 0.0005 0 0.0002 0 0.0001 0.0001 0.0001 0 – – – – – –
Endoplasmic Reticulum 0 0 0 0.0002 0.0005 0 0.0038 0 0.0003 0 0.0001 0 – – – – – –
Endosome 0.0001 0 0 0.0179 0.0174 0 0.0445 0 0.0084 0.0024 0.004 0 – – – – – –
Golgi 0.0001 0 0 0.0108 0.0096 0 0.0214 0 0.0027 0.003 0.0074 0 – – – – – –
Lipid Particles 0.0005 0.0001 0.0003 0.0532 0.0279 0.0003 0.0687 0.0014 0.0154 0.0802 0.0316 0.0008 – – – – – –
Mitochondria 0.0006 0.0002 0.0003 0.0071 0.0234 0.0001 0.0153 0.0004 0.0067 0.0095 0.0197 0.0018 – – – – – –
None 0.0001 0 0 0.0001 0.0007 0 0.0076 0 0.0009 0.0002 0.0002 0 – – – – – –
Nuclear Periphery 0.0005 0.0001 0 0.0005 0.0114 0 0.0419 0 0.0015 0.0002 0.0015 0 – – – – – –
Nucleolus 0.7948 0.8013 0.9392 0.8321 0.6533 0.9444 0.5187 0.8943 0.8684 0.8316 0.8234 0.9145 – – – – – –
Nucleus 0.1793 0.1968 0.0581 0.0589 0.0532 0.0542 0.1514 0.1022 0.0475 0.0447 0.0639 0.0812 – – – – – –
Peroxisomes 0 0 0.0001 0.0027 0.0052 0 0.0101 0.0001 0.0056 0.0138 0.0053 0.0002 – – – – – –
Punctate Nuclear 0.021 0.0005 0.0002 0.0009 0.0608 0.0004 0.0286 0.0004 0.0136 0.0026 0.0239 0.0005 – – – – – –
Vacuole 0.0006 0.0001 0.0003 0.0042 0.0032 0.0001 0.0031 0.0003 0.0011 0.0005 0.0011 0.0001 – – – – – –
Vacuole Periphery 0.0003 0.0001 0 0.002 0.0018 0 0.0035 0.0001 0.001 0.0003 0.0031 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.9675 33.8011 26.906 29.9864 46.35 22.6743 25.3215 25.1636 20.9069 33.6989
Translational Efficiency 2.8437 3.2473 2.954 2.4412 2.0643 3.0814 1.8502 1.5494 1.7583 2.1461

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the SSU and 90S preribosomes; involved in pre-18S rRNA processing; binds to U3 snoRNA and Mpp10p; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
Localization
Cell Percentages nucleolus (89%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Bfr2

Bfr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bfr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available