Standard name
Human Ortholog
Description Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; required for nitrogen starvation-induced ribophagy but not for nonselective autophagy; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.08 0.05 0.08 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.97 0.94 0.92 0.81 0.73 0.75 0.7 0.69 0.74 1.0 0.99 0.99 0.94 0.98 0.95 0.95 0.97 0.93 0.96 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0 0.05 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.09 0.12 0.15 0.2 0.24 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.14 0.09 0.06 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 1 0 1 2 3 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 3 3 3 4 2 0 0 0 0 0 0 0 0 0 0 0 4 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 5 3 2 4 7 10 6 7 9 9 12 10 11 8 18 16 18 0 0 0 0 0 0
Cytoplasm 263 220 162 174 218 238 280 242 240 236 205 192 446 515 518 207 309 224 263 212 149 251 465 398
Endoplasmic Reticulum 0 5 3 5 5 4 19 7 17 24 11 14 2 4 3 4 6 10 0 0 3 0 5 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 2 0 1 0 0 0 0 1
Mitochondria 2 0 0 0 2 10 30 39 47 68 71 51 0 0 0 0 2 1 1 1 1 1 1 1
Nucleus 0 0 0 0 1 0 4 0 2 4 4 3 0 1 0 5 0 0 1 1 0 3 1 2
Nuclear Periphery 0 0 0 0 0 0 2 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 2 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 0 0 0 5 7 16 46 28 21 25 12 0 0 1 4 1 9 0 0 2 1 4 3
Unique Cell Count 269 225 164 179 231 258 345 332 321 336 295 261 448 519 521 220 315 235 277 219 161 263 490 420
Labelled Cell Count 273 230 168 181 235 266 368 345 344 367 331 288 458 531 531 240 336 263 277 219 161 263 490 420


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.5 27.5 20.4 19.6 16.2 13.7 12.3 11.5 10.7 10.5 9.7 9.5 25.0 24.1 22.2 31.0 29.2 29.5 24.2 24.4 23.7
Std Deviation (1e-4) 4.2 4.3 4.2 3.3 2.5 2.7 2.4 2.4 2.3 2.3 2.3 2.0 4.6 3.9 3.8 8.9 7.3 8.5 4.6 5.2 6.1
Intensity Change (Log2) -0.05 -0.33 -0.58 -0.73 -0.82 -0.93 -0.95 -1.07 -1.1 0.29 0.24 0.13 0.6 0.52 0.53 0.25 0.26 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1.5 0 0 0 2.7 1.7 2.6
Cytoplasm -1.0 -2.3 -2.9 -5.5 -7.0 -6.6 -7.4 -7.5 -6.8 1.1 0.5 0.8 -2.3 -0.6 -1.9
Endoplasmic Reticulum 0 0 0 1.9 0 1.8 2.5 1.1 1.8 0 0 0 0 0 1.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.9 4.6 5.2 6.2 6.8 6.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.8 5.0 3.9 3.3 3.8 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.2321 24.0017 20.8983 19.1795 20.2142 21.2687 15.2775 25.6351 23.2428 20.1661 13.6025 22.1177 21.8983 29.4186 25.1908 24.5226 20.4974 26.1611
Actin 0.0113 0.0003 0.0075 0.0172 0.0478 0.0037 0.0618 0.0001 0.0091 0.0036 0.1382 0.0009 0.002 0.0006 0.004 0 0.0246 0.0025
Bud 0.0002 0.0001 0.0002 0.0006 0.0003 0.0001 0.0033 0 0.0005 0.0149 0.0006 0.0002 0.0005 0.0003 0.0002 0.0003 0.0009 0.0003
Bud Neck 0.0042 0.0002 0.0004 0.0012 0.003 0.0007 0.0017 0.0001 0.0003 0.0005 0.0009 0.0007 0.0003 0.0001 0.0017 0.0004 0.0012 0.0007
Bud Periphery 0.0001 0 0.0002 0.0007 0.0005 0.0001 0.0034 0 0.0006 0.0028 0.0006 0.0001 0.0005 0.0002 0.0005 0.0001 0.0014 0.0004
Bud Site 0.001 0.0005 0.0003 0.001 0.0007 0.0001 0.0086 0.0001 0.0067 0.0014 0.0016 0.0001 0.0008 0.0009 0.0008 0.0001 0.0034 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0004 0 0.0001 0.0002 0.0002 0.0001 0.0003 0 0.0003 0.0001 0.0004 0
Cytoplasm 0.918 0.9875 0.9644 0.9409 0.9233 0.9778 0.7248 0.9948 0.9594 0.8736 0.6678 0.9596 0.8989 0.9953 0.9555 0.9597 0.7619 0.9679
Cytoplasmic Foci 0.0128 0.0014 0.0022 0.0024 0.0015 0.002 0.0291 0.0004 0.0026 0.0071 0.0272 0.0045 0.0146 0.0005 0.0038 0.0126 0.0133 0.0028
Eisosomes 0.0001 0 0.0001 0.0002 0.0003 0 0.0005 0 0.0001 0 0.0003 0 0 0 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.0057 0.0011 0.002 0.0055 0.0011 0.0047 0.0067 0.0003 0.0029 0.0006 0.0231 0.0055 0.0043 0.0004 0.0015 0.0009 0.0314 0.0037
Endosome 0.0128 0.002 0.005 0.0063 0.005 0.0016 0.0415 0.0001 0.0021 0.0269 0.0551 0.0139 0.0197 0.0002 0.0074 0.0118 0.0414 0.0052
Golgi 0.0022 0.0003 0.0008 0.0019 0.0045 0.0008 0.0167 0 0.0015 0.0148 0.0122 0.0029 0.0041 0.0001 0.005 0.0003 0.0229 0.0023
Lipid Particles 0.0024 0.001 0.0009 0.0006 0.002 0.0015 0.0215 0 0.0008 0.0338 0.0161 0.0005 0.0065 0 0.0051 0.0011 0.0259 0.0023
Mitochondria 0.0005 0.0004 0.0035 0.0015 0.0011 0.0012 0.0247 0 0.0057 0.0084 0.0115 0.001 0.0166 0.0002 0.0039 0.0001 0.0163 0.0035
None 0.004 0.0003 0.0006 0.0021 0.0004 0.0005 0.0058 0.0001 0.0004 0.0004 0.0042 0.0004 0.0013 0.0001 0.0003 0.0024 0.0029 0.0003
Nuclear Periphery 0.0067 0.001 0.0026 0.0069 0.0027 0.0011 0.0137 0.0007 0.0014 0.0008 0.0111 0.0017 0.0054 0.0002 0.0027 0.0006 0.0164 0.0016
Nucleolus 0.0008 0.0004 0.0007 0.0003 0.0003 0 0.0019 0 0.0001 0.0002 0.0003 0 0.0005 0 0.0001 0.0004 0.002 0.0001
Nucleus 0.0079 0.0019 0.0036 0.0054 0.0019 0.0023 0.0067 0.0028 0.002 0.0021 0.0067 0.0028 0.0116 0.0007 0.0027 0.0025 0.0089 0.0023
Peroxisomes 0.0011 0.0001 0.0002 0.0002 0.0005 0.0005 0.0128 0 0.0024 0.0049 0.0097 0.0001 0.0022 0 0.001 0.0001 0.0051 0.0021
Punctate Nuclear 0.0047 0.0008 0.001 0.0023 0.0013 0.0004 0.004 0.0001 0.0002 0.0005 0.0065 0.0003 0.0028 0 0.0014 0.0009 0.0052 0.0005
Vacuole 0.0029 0.0006 0.0024 0.0019 0.0011 0.0007 0.0077 0.0001 0.0007 0.0016 0.0045 0.0036 0.0053 0.0002 0.0014 0.0053 0.01 0.0007
Vacuole Periphery 0.0007 0.0001 0.0013 0.0008 0.0005 0.0003 0.0028 0 0.0004 0.0009 0.0018 0.0012 0.0018 0.0001 0.0005 0.0005 0.004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 63.4436 58.2475 50.0467 58.7701 62.8046 36.8455 65.2871 64.3314 52.5548 69.5107
Translational Efficiency 1.2664 1.4341 1.4146 1.1114 1.0091 2.0206 1.1848 0.95 1.0979 1.0482

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; required for nitrogen starvation-induced ribophagy but not for nonselective autophagy; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Pro1

Pro1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pro1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available