Standard name
Human Ortholog
Description RING-type ubiquitin ligase of the endosomal and vacuolar membranes; binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.47 0.23 0.18 0.14 0.19 0.36 0.42 0.45 0.47 0.5 0.57 0.56 0.08 0.15 0.25 0.11 0.2 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.16 0.14 0.21 0.2 0.19 0.05 0 0 0 0 0 0 0.08 0.18 0.16 0.11 0.05 0.11 0.11 0.2 0.25 0.25 0.22 0.22
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Mitochondria 0.31 0 0.17 0.38 0.25 0.41 0.51 0.46 0.5 0.41 0.45 0.47 0 0 0 0 0.05 0 0.06 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.3 0.79 0.69 0.49 0.61 0.37 0.25 0.26 0.25 0.23 0.18 0.21 0.85 0.8 0.71 0.89 0.85 0.79 0.71 0.71 0.59 0.62 0.6 0.65
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 1 0 1 0
Bud 0 0 2 1 1 5 7 7 5 5 7 5 0 0 0 0 0 0 0 0 1 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 1 0 0 0 0 1 1 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 41 43 32 12 47 76 153 123 140 168 147 186 6 34 48 2 4 5 3 6 8 0 4 6
Endoplasmic Reticulum 3 0 4 2 1 8 18 4 7 12 1 10 2 1 7 0 0 1 1 0 0 0 1 3
Endosome 14 26 38 17 48 11 10 7 9 9 4 2 6 40 31 2 1 3 11 40 46 23 39 51
Golgi 3 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 1 1 0
Mitochondria 27 1 30 32 63 86 188 126 149 138 116 156 0 0 0 0 1 0 5 0 5 0 4 4
Nucleus 0 4 1 0 5 5 1 0 0 2 1 1 0 3 5 0 0 1 0 3 0 1 4 5
Nuclear Periphery 0 0 1 0 5 0 2 2 0 0 0 1 0 0 1 0 0 0 3 2 5 2 3 2
Nucleolus 0 5 8 2 3 2 0 0 0 0 0 0 1 4 1 0 0 0 0 0 0 0 2 1
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
SpindlePole 0 3 1 2 4 3 0 1 3 1 0 0 0 2 1 0 0 0 0 0 2 1 1 2
Vac/Vac Membrane 26 147 125 41 154 78 92 70 73 77 46 69 60 176 137 16 17 22 70 142 109 57 108 149
Unique Cell Count 88 186 181 84 251 210 366 274 297 333 258 334 71 220 194 18 20 28 100 201 187 92 180 231
Labelled Cell Count 117 229 243 109 331 275 471 341 388 415 322 432 76 260 232 21 23 32 100 201 187 92 180 231


Ambiguous, Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 7.1 5.3 5.1 5.3 5.2 4.5 4.6 4.8 4.6 4.5 4.5 7.3 7.1 7.4 8.2 9.2 8.5 7.8 8.1 8.1
Std Deviation (1e-4) 2.2 1.3 1.2 1.6 1.5 1.3 1.0 1.1 1.3 1.1 1.0 1.0 1.1 1.4 1.4 2.2 2.1 2.0 2.0 2.0 1.4
Intensity Change (Log2) – – – -0.05 0.0 -0.03 -0.24 -0.18 -0.12 -0.21 -0.22 -0.21 0.47 0.43 0.49 0.63 0.8 0.7 0.57 0.63 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 0.3 4.1 5.6 6.0 6.5 7.3 8.2 8.3 -1.8 -0.6 1.7 0 0 0
Endoplasmic Reticulum 0 0 0.9 0 0 0 0 0 0 0 0 0.8 0 0 0
Endosome -0.1 -0.5 -4.7 0 0 0 0 0 0 -2.4 -0.7 -1.3 0 0 -1.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.9 2.1 5.3 0 0 0 0 0 0 -3.7 -6.3 -5.9 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 -1.5 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.2 -1.7 -6.3 -9.9 -9.2 -9.6 -10.2 -10.8 -10.8 2.5 2.5 0.3 1.8 1.5 1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8986 2.3235 2.3768 1.6824 1.6465 2.0832 2.7156 3.4608 3.1786 2.2961 2.9693 3.313 0.1131 1.43 0.712 1.1457 0.5452 0.6824
Actin 0.0271 0.0006 0.0214 0.0008 0.0002 0.0119 0.012 0 0.0147 0.0075 0.0016 0.0089 0.0028 0 0.0003 0.0023 0.0022 0.002
Bud 0.0019 0.017 0.0026 0.0074 0.0005 0.0026 0.0011 0.0003 0.0012 0.0028 0.0003 0.0004 0.0009 0.0003 0.0003 0.0002 0.0004 0.0001
Bud Neck 0.0013 0.0022 0.0007 0.0002 0.0003 0.0013 0.0049 0.0003 0.0022 0.0011 0.0003 0.0015 0.0005 0.0005 0.0005 0.0007 0.0008 0.0009
Bud Periphery 0.003 0.0034 0.0028 0.023 0.0006 0.0024 0.0012 0.0004 0.0017 0.0038 0.0003 0.0005 0.0005 0.0002 0.0002 0.0002 0.0008 0.0002
Bud Site 0.0038 0.0162 0.0097 0.0004 0.0004 0.0032 0.0097 0.0013 0.0031 0.0007 0.0018 0.0005 0.0011 0.0013 0.001 0.0035 0.0007 0.0003
Cell Periphery 0.0008 0.0006 0.0005 0.0002 0.0002 0.0005 0.0008 0.0002 0.0004 0.0002 0.0001 0.0002 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.065 0.1257 0.1047 0.1331 0.0177 0.0741 0.0514 0.0427 0.0408 0.0451 0.019 0.0517 0.0479 0.0533 0.0435 0.0339 0.0184 0.0271
Cytoplasmic Foci 0.0455 0.0267 0.0423 0.0197 0.0638 0.0612 0.0465 0.0321 0.0557 0.0548 0.043 0.0458 0.0483 0.0394 0.0488 0.0376 0.0281 0.0322
Eisosomes 0.0002 0 0.0001 0 0 0 0.0005 0 0.0002 0.0001 0 0 0.0001 0 0 0 0 0.0001
Endoplasmic Reticulum 0.014 0.0098 0.01 0.0054 0.0012 0.0056 0.0082 0.0052 0.0102 0.0018 0.0015 0.005 0.0044 0.0075 0.0032 0.0015 0.0057 0.0033
Endosome 0.3746 0.2725 0.3344 0.3406 0.4736 0.3102 0.4845 0.4437 0.4493 0.3762 0.5637 0.4288 0.4744 0.3995 0.4651 0.5115 0.5268 0.4616
Golgi 0.0049 0.0044 0.0067 0.0137 0.0066 0.0052 0.0152 0.0061 0.0151 0.026 0.0126 0.0099 0.0062 0.0067 0.0071 0.0102 0.0091 0.0058
Lipid Particles 0.014 0.0066 0.0099 0.0017 0.0081 0.0056 0.0163 0.0054 0.0075 0.0054 0.0269 0.0065 0.0131 0.0107 0.0087 0.0055 0.0067 0.0121
Mitochondria 0.0075 0.0018 0.0097 0.0025 0.0037 0.0036 0.0081 0.001 0.0116 0.0224 0.0099 0.004 0.0044 0.0015 0.0038 0.0015 0.007 0.0026
None 0.1544 0.0781 0.0726 0.0039 0.0276 0.0143 0.0517 0.0284 0.0373 0.0146 0.0035 0.0399 0.0418 0.0231 0.0284 0.0287 0.0117 0.0074
Nuclear Periphery 0.0329 0.0176 0.0167 0.0092 0.0038 0.0112 0.0203 0.0136 0.012 0.0075 0.0049 0.0126 0.0153 0.0234 0.0111 0.0026 0.0199 0.0166
Nucleolus 0.0021 0.0023 0.0017 0.0002 0.0009 0.0024 0.0014 0.0007 0.0012 0.0003 0.0003 0.001 0.0018 0.0012 0.0043 0.0005 0.0012 0.001
Nucleus 0.0138 0.0054 0.0057 0.0054 0.001 0.0064 0.0063 0.0033 0.005 0.0012 0.0007 0.0068 0.0139 0.0041 0.0055 0.0039 0.0036 0.0063
Peroxisomes 0.0012 0.001 0.0014 0.0004 0.0067 0.0193 0.0105 0.0005 0.0058 0.0087 0.0152 0.0068 0.001 0.0005 0.0009 0.0051 0.0012 0.0009
Punctate Nuclear 0.0031 0.0008 0.0024 0.0007 0.0013 0.0055 0.0023 0.0003 0.001 0.0003 0.0019 0.0029 0.0062 0.0008 0.0014 0.0007 0.0011 0.0007
Vacuole 0.1861 0.3147 0.2774 0.2701 0.2516 0.3274 0.2048 0.3469 0.2445 0.2551 0.19 0.2654 0.2622 0.3194 0.2826 0.2914 0.2562 0.2962
Vacuole Periphery 0.0428 0.0925 0.0663 0.1615 0.1302 0.1261 0.0423 0.0675 0.0795 0.1642 0.1023 0.1008 0.053 0.1065 0.0831 0.0584 0.0982 0.1223

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.6115 12.6018 5.9507 13.5284 14.9541 7.685 16.5062 21.1085 20.2005 18.0552
Translational Efficiency 0.858 0.7979 1.1832 0.6086 0.6258 1.5464 0.745 0.7426 0.587 0.6365

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RING-type ubiquitin ligase of the endosomal and vacuolar membranes; binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain
Localization
Cell Percentages vacuolar membrane (50%), vacuole (15%), mixed (30%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pib1

Pib1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pib1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available