Standard name
Human Ortholog
Description Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0.14 0.1 0.12 0.09 0.08 0.07 – 0 0.08 0 0 0.1 0.11 0 0.11 0.11 0.09 0 0.07 0 0.05 0 0
Bud 0 0 0 0 0 0 0 – 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.26 0.25 0.35 0.14 0.23 0.26 0.31 – 0.18 0.2 0.13 0.22 0.5 0.45 0.5 0.21 0.22 0.25 0.06 0.11 0 0.12 0.13 0.17
Endoplasmic Reticulum 0.15 0.25 0.12 0.19 0.13 0.11 0.13 – 0.06 0.09 0.08 0.09 0.2 0.16 0.08 0 0.05 0.07 0 0.07 0 0 0.05 0.08
Endosome 0.09 0.05 0.18 0.1 0.23 0.07 0.1 – 0.07 0 0 0 0.1 0.16 0.18 0.11 0.08 0.15 0.16 0.13 0.15 0.16 0.14 0.13
Golgi 0.24 0.27 0.07 0.18 0.14 0.08 0.05 – 0 0 0 0 0.07 0.12 0.16 0.34 0.39 0.28 0.47 0.32 0.24 0.13 0.22 0.17
Mitochondria 0.45 0.2 0 0.35 0.18 0.44 0.42 – 0.69 0.63 0.88 0.65 0 0 0 0.11 0.1 0.1 0.12 0.13 0.32 0.27 0.23 0.2
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.08 0.18 0.08 0.15 0.11 0.05 – 0.08 0.07 0 0.08 0.06 0.08 0.12 0.26 0.25 0.26 0.07 0.13 0.14 0.16 0.12 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 32 7 12 10 17 17 – 10 19 9 10 17 19 6 29 24 20 7 22 5 6 9 9
Bud 2 2 1 2 1 0 3 – 12 9 19 9 0 1 0 1 3 5 2 9 0 2 2 8
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 2 1 2 3
Bud Site 0 0 0 0 0 1 0 – 1 1 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 1 3 1 2 3 – 0 3 1 4 0 1 2 4 3 5 0 0 0 0 0 0
Cytoplasm 39 57 24 15 25 53 77 – 45 50 35 72 83 77 121 58 49 54 11 36 3 14 31 47
Endoplasmic Reticulum 23 56 8 20 14 22 34 – 15 23 23 28 34 28 19 12 11 15 7 21 3 3 13 22
Endosome 13 11 12 10 25 14 26 – 18 8 3 12 16 27 44 29 18 33 31 41 20 19 33 36
Golgi 37 60 5 19 16 16 13 – 3 8 3 4 12 20 38 93 87 61 90 105 32 16 52 48
Mitochondria 69 45 2 36 20 88 105 – 175 154 245 214 4 5 10 31 22 21 23 42 42 33 56 55
Nucleus 0 0 1 4 0 0 3 – 3 3 2 7 5 4 1 1 0 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 1 0 – 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 1
Nucleolus 0 1 0 0 0 0 0 – 1 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 1 0 – 3 0 0 1 0 0 2 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 4 2 – 2 4 2 4 3 7 3 8 5 4 2 2 0 0 3 2
Vac/Vac Membrane 9 18 12 8 17 23 13 – 20 17 11 25 10 13 28 71 55 56 13 41 19 20 28 39
Unique Cell Count 152 224 68 104 111 202 252 252 244 277 327 166 173 243 270 224 215 193 332 136 124 239 282
Labelled Cell Count 206 284 74 130 132 242 296 308 299 356 394 185 202 274 338 278 274 193 332 136 124 239 282


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 9.3 9.1 7.7 8.2 7.2 7.1 – 6.1 6.2 5.5 5.9 8.8 8.4 8.6 11.1 11.3 10.7 8.2 8.5 9.0
Std Deviation (1e-4) 0.9 1.5 1.4 1.4 1.4 1.9 1.6 – 1.5 1.4 1.2 1.3 1.3 1.0 1.3 1.9 2.4 2.4 1.2 1.3 1.6
Intensity Change (Log2) – – – -0.23 -0.15 -0.34 -0.36 – -0.58 -0.54 -0.72 -0.63 -0.05 -0.11 -0.07 0.29 0.31 0.24 -0.15 -0.1 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 -0.3 -0.5 -1.0 – 0 -0.7 0 0 0 0.2 0 0.1 0.1 -0.2
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.2 -1.9 -1.4 -0.7 – -3.1 -2.5 -4.4 -2.3 2.1 1.3 2.1 -2.4 -2.2 -1.6
Endoplasmic Reticulum 1.3 0.2 -0.2 0.4 – 0 -0.6 -0.9 -0.8 1.6 0.9 -1.0 0 0 -1.3
Endosome -1.5 0.8 -2.6 -1.7 – -2.6 0 0 0 -1.7 -0.4 0.1 -1.6 -2.3 -0.5
Golgi 2.0 1.4 0.2 0 – 0 0 0 0 0 1.0 1.7 4.4 4.9 3.6
Mitochondria 4.9 3.0 6.1 6.0 – 9.8 8.8 14.0 9.4 0 0 0 2.1 1.8 1.8
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -2.0 -0.4 -1.3 -3.4 – -2.4 -2.7 0 -2.6 -2.8 -2.3 -1.3 1.5 1.2 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2704 2.4442 1.9809 1.8164 1.7184 2.1043 2.2205 3.3872 2.8536 2.7793 2.4549 3.0999 2.667 3.5768 3.1119 2.9386 2.7801 3.0564
Actin 0.0306 0.0117 0.0175 0.0671 0.0024 0.0064 0.0149 0.0021 0.0126 0.0105 0.0034 0.0044 0.0092 0.0043 0.0019 0.002 0.0015 0.002
Bud 0.0009 0.0013 0.0026 0.0029 0.0008 0.0007 0.0011 0.0029 0.0106 0.0049 0.0014 0.0015 0.0009 0.0016 0.0013 0.0035 0.0005 0.0005
Bud Neck 0.0036 0.0012 0.0011 0.0035 0.0005 0.0045 0.0007 0.0006 0.0019 0.0043 0.0005 0.0013 0.0009 0.0006 0.0005 0.0004 0.0005 0.0011
Bud Periphery 0.0014 0.0012 0.002 0.003 0.0004 0.0008 0.0009 0.0012 0.0062 0.0023 0.0006 0.001 0.0006 0.0005 0.0006 0.0058 0.0002 0.0003
Bud Site 0.0101 0.0092 0.0167 0.0086 0.0012 0.0012 0.0048 0.0107 0.0077 0.0025 0.0008 0.0005 0.005 0.0104 0.0017 0.0011 0.0005 0.0005
Cell Periphery 0.0019 0.0017 0.0009 0.0009 0.0001 0.0003 0.0004 0.0002 0.0006 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001
Cytoplasm 0.0935 0.184 0.1016 0.0303 0.0897 0.1111 0.1291 0.2565 0.1078 0.0904 0.038 0.1104 0.0853 0.152 0.1068 0.0743 0.073 0.0949
Cytoplasmic Foci 0.1218 0.1569 0.108 0.1286 0.1677 0.1509 0.1376 0.1522 0.1617 0.1326 0.1201 0.1641 0.138 0.1698 0.1531 0.1032 0.1246 0.1444
Eisosomes 0.0009 0.0004 0.0009 0.0006 0 0 0.0002 0 0.0001 0.0001 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0136 0.006 0.0101 0.002 0.0018 0.0044 0.0109 0.0045 0.0063 0.0033 0.0029 0.003 0.0057 0.0038 0.0031 0.0024 0.0025 0.0055
Endosome 0.4314 0.3682 0.4745 0.4648 0.5141 0.4275 0.4381 0.4132 0.4389 0.5458 0.6133 0.4582 0.5361 0.4775 0.5272 0.6392 0.6518 0.4894
Golgi 0.1805 0.187 0.1804 0.1624 0.1782 0.2131 0.1471 0.1147 0.1711 0.1319 0.1442 0.1883 0.1641 0.1455 0.1608 0.1166 0.1077 0.2096
Lipid Particles 0.0197 0.005 0.0069 0.0088 0.0105 0.0068 0.0115 0.001 0.0045 0.0051 0.0036 0.0017 0.0041 0.001 0.0012 0.0006 0.0037 0.0014
Mitochondria 0.016 0.0301 0.0357 0.0649 0.0097 0.0452 0.0403 0.0115 0.0248 0.0193 0.0253 0.0355 0.0212 0.0119 0.0224 0.023 0.0082 0.0329
None 0.0203 0.0166 0.0091 0.0005 0.0011 0.0009 0.0119 0.0093 0.0158 0.0154 0.0152 0.0061 0.0109 0.0023 0.0009 0.0007 0.0005 0.0016
Nuclear Periphery 0.0103 0.001 0.003 0.0005 0.0004 0.0006 0.0084 0.0012 0.0016 0.0013 0.0012 0.0008 0.0008 0.0006 0.0006 0.0007 0.001 0.0008
Nucleolus 0.001 0.0003 0.0004 0.0002 0.0002 0.002 0.0003 0.0002 0.0003 0.0004 0.0022 0.0004 0.0001 0.0001 0 0.0001 0 0
Nucleus 0.0026 0.0006 0.0008 0.0005 0.0003 0.0012 0.0023 0.0007 0.0013 0.0015 0.0011 0.0004 0.0005 0.0005 0.0003 0.0004 0.0004 0.0005
Peroxisomes 0.015 0.0024 0.009 0.0366 0.0052 0.0067 0.0106 0.0018 0.0074 0.0066 0.0085 0.009 0.0035 0.0046 0.0048 0.0014 0.0034 0.0044
Punctate Nuclear 0.0095 0.0004 0.0005 0.0004 0.0005 0.0005 0.003 0.0004 0.0026 0.002 0.0017 0.0004 0.0004 0.0003 0.0003 0.0002 0.0004 0.0003
Vacuole 0.0106 0.0109 0.0123 0.0083 0.0097 0.0099 0.0199 0.0113 0.0108 0.0131 0.0079 0.0074 0.0083 0.0083 0.0073 0.0159 0.0108 0.0056
Vacuole Periphery 0.0047 0.004 0.0062 0.0046 0.0054 0.0049 0.006 0.0037 0.0054 0.0065 0.0079 0.0054 0.0042 0.0046 0.0051 0.0085 0.0087 0.0044

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9436 12.7782 10.2113 16.3243 16.0383 12.0884 17.5165 14.4217 16.7192 18.765
Translational Efficiency 2.1598 2.1549 2.6836 1.6947 1.5758 2.684 1.5362 1.9067 1.6498 1.6158

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles
Localization
Cell Percentages cytoplasm (8%), Golgi (7%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Swa2

Swa2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swa2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available