Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 5 | 7 | 4 | 1 | 0 | 1 | 8 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 3 | 1 | – | 3 | 7 | 3 | 4 | 6 | 5 |
Bud | 3 | 2 | 2 | 6 | 6 | 1 | 12 | 21 | 12 | 9 | 6 | 13 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 3 | 4 | 0 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 4 | 12 | 9 | 6 | 4 | 2 | 4 | 7 | 3 | 5 | 1 | 1 | 2 | 4 | 7 | 3 | 3 | – | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 2 | 1 | 7 | 8 | 20 | 15 | 12 | 15 | 4 | 11 | 9 | 7 | 2 | 9 | 9 | 0 | 4 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 4 | 3 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 4 | 2 | 4 | 4 | 0 | 1 | – | 0 | 1 | 0 | 0 | 1 | 2 |
Endosome | 1 | 1 | 5 | 5 | 20 | 26 | 10 | 12 | 3 | 0 | 0 | 3 | 2 | 3 | 10 | 1 | 0 | – | 3 | 7 | 8 | 6 | 8 | 11 |
Golgi | 0 | 23 | 37 | 29 | 43 | 6 | 1 | 2 | 0 | 0 | 0 | 2 | 25 | 65 | 41 | 14 | 17 | – | 8 | 20 | 13 | 5 | 23 | 16 |
Mitochondria | 193 | 209 | 179 | 205 | 288 | 267 | 418 | 409 | 325 | 265 | 293 | 307 | 106 | 121 | 148 | 2 | 1 | – | 171 | 197 | 190 | 97 | 178 | 202 |
Nucleus | 0 | 1 | 1 | 1 | 1 | 1 | 4 | 9 | 9 | 2 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 12 | 8 | 9 | 7 | 13 | 28 | 30 | 37 | 26 | 21 | 25 | 20 | 6 | 4 | 4 | 1 | 1 | – | 0 | 0 | 1 | 0 | 2 | 2 |
Nucleolus | 9 | 4 | 6 | 2 | 9 | 18 | 44 | 33 | 42 | 20 | 24 | 20 | 8 | 2 | 5 | 2 | 2 | – | 0 | 1 | 0 | 3 | 0 | 2 |
Peroxisomes | 0 | 3 | 4 | 5 | 9 | 7 | 6 | 12 | 7 | 2 | 1 | 1 | 1 | 4 | 9 | 1 | 1 | – | 7 | 4 | 10 | 13 | 17 | 8 |
SpindlePole | 0 | 1 | 3 | 2 | 6 | 8 | 10 | 17 | 5 | 2 | 1 | 1 | 2 | 0 | 2 | 1 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 29 | 21 | 10 | 36 | 30 | 33 | 22 | 16 | 14 | 13 | 20 | 7 | 13 | 30 | 7 | 7 | – | 0 | 5 | 2 | 3 | 4 | 12 |
Unique Cell Count | 199 | 234 | 215 | 229 | 358 | 336 | 470 | 479 | 361 | 294 | 314 | 330 | 120 | 155 | 196 | 29 | 34 | 197 | 248 | 235 | 138 | 247 | 272 | |
Labelled Cell Count | 229 | 303 | 293 | 290 | 458 | 412 | 589 | 609 | 457 | 352 | 385 | 402 | 164 | 231 | 269 | 37 | 39 | 197 | 248 | 235 | 138 | 247 | 272 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 10.9 | 8.9 | 8.6 | 8.8 | 7.6 | 7.2 | 7.1 | 6.1 | 6.4 | 5.9 | 6.4 | 8.9 | 9.2 | 9.2 | 10.7 | 10.9 | – | 9.1 | 9.4 | 9.7 |
Std Deviation (1e-4) | 1.3 | 1.8 | 1.3 | 1.6 | 1.6 | 1.9 | 1.8 | 1.7 | 1.5 | 1.9 | 1.6 | 1.6 | 1.8 | 1.3 | 1.8 | 2.5 | 2.4 | – | 1.8 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.02 | -0.24 | -0.31 | -0.33 | -0.55 | -0.48 | -0.6 | -0.47 | -0.0 | 0.05 | 0.05 | 0.27 | 0.29 | – | 0.03 | 0.08 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud | 0 | 0 | 0 | 0 | 2.4 | 1.8 | 0 | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | -0.9 | 0 | 0 | 0 | 0 | 0 | -1.7 | 0 | 0 | 0 | -0.8 | -0.3 | 0 | 0 | – |
Cytoplasm | 0.1 | 1.3 | 0.7 | -0.5 | -0.1 | 0 | 0.3 | -0.3 | -0.8 | 0 | 1.2 | 0.7 | 0 | 0 | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Endosome | 0 | 1.9 | 2.7 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 0 | – |
Golgi | -1.3 | -1.7 | -6.6 | -9.0 | -8.9 | -8.1 | -7.4 | -7.6 | -7.3 | 0.8 | 5.3 | 1.0 | 3.9 | 4.3 | – |
Mitochondria | 1.9 | -0.8 | -1.1 | 2.1 | 0.7 | 2.4 | 2.3 | 3.7 | 3.6 | 1.3 | -1.3 | -1.9 | -8.8 | -9.7 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | -0.6 | -0.3 | 1.9 | 1.2 | 1.7 | 1.5 | 1.4 | 1.7 | 1.0 | 0.3 | -0.8 | -1.2 | 0 | 0 | – |
Nucleolus | 0 | -0.2 | 1.4 | 3.1 | 2.2 | 3.7 | 2.0 | 2.4 | 1.8 | 1.7 | 0 | -0.2 | 0 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Vacuole | -2.2 | 0.1 | -0.3 | -1.2 | -2.6 | -2.5 | -2.2 | -2.6 | -1.6 | -1.2 | -0.5 | 1.7 | 0 | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.4253 | 8.6533 | 8.4551 | 7.9215 | 7.0388 | 8.8321 | 7.0768 | 7.376 | 7.2556 | 6.6314 | 6.3361 | 7.3205 | 6.9828 | 7.1377 | 6.4984 | 5.882 | 6.1603 | 6.4063 |
Actin | 0.0168 | 0.0103 | 0.0062 | 0.0002 | 0.0188 | 0.0003 | 0.0109 | 0.0023 | 0.0075 | 0.0029 | 0.0213 | 0.0004 | 0.0299 | 0.0046 | 0.012 | 0.0003 | 0.0017 | 0.0064 |
Bud | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0 | 0.0001 | 0.0007 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0004 | 0.0006 | 0.0002 | 0 | 0.0001 | 0.001 |
Bud Neck | 0.0004 | 0.0001 | 0 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0012 | 0 | 0.0002 | 0 | 0.0016 | 0.0002 | 0.0008 | 0 | 0.0001 | 0.0001 |
Bud Periphery | 0.0005 | 0.0002 | 0.0001 | 0.002 | 0.0007 | 0 | 0.0003 | 0.001 | 0.0004 | 0.0001 | 0.0013 | 0 | 0.0007 | 0.0008 | 0.0003 | 0.0001 | 0.0002 | 0.0018 |
Bud Site | 0.0011 | 0.0006 | 0.0004 | 0.0004 | 0.0017 | 0 | 0.0008 | 0.0061 | 0.002 | 0.0001 | 0.0008 | 0 | 0.0042 | 0.0042 | 0.0012 | 0 | 0.0003 | 0.0007 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0013 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 |
Cytoplasm | 0.0009 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0005 | 0.0001 | 0.0039 | 0.0003 | 0.0003 | 0 | 0.0018 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0003 |
Cytoplasmic Foci | 0.0089 | 0.0037 | 0.0031 | 0.0014 | 0.0052 | 0.0002 | 0.0061 | 0.0027 | 0.009 | 0.007 | 0.01 | 0.0005 | 0.0122 | 0.0034 | 0.0078 | 0.0077 | 0.0052 | 0.0008 |
Eisosomes | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0009 | 0 | 0 | 0 | 0.0018 | 0 | 0.0003 | 0 | 0.002 | 0.004 | 0.0004 | 0 | 0.0023 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 |
Endosome | 0.0121 | 0.0046 | 0.0019 | 0.0012 | 0.0095 | 0.0014 | 0.0091 | 0.0057 | 0.0165 | 0.0146 | 0.0127 | 0.003 | 0.0175 | 0.0098 | 0.0045 | 0.002 | 0.0128 | 0.0031 |
Golgi | 0.0126 | 0.0151 | 0.0037 | 0.0009 | 0.0086 | 0.0112 | 0.0115 | 0.0185 | 0.0153 | 0.0261 | 0.0304 | 0.0091 | 0.0232 | 0.0225 | 0.0154 | 0.0034 | 0.0171 | 0.0144 |
Lipid Particles | 0.0287 | 0.0098 | 0.0094 | 0.0159 | 0.0235 | 0.0052 | 0.0179 | 0.0088 | 0.0203 | 0.0214 | 0.0108 | 0.0031 | 0.0199 | 0.0114 | 0.0067 | 0.0031 | 0.0042 | 0.0049 |
Mitochondria | 0.8667 | 0.9288 | 0.9678 | 0.9361 | 0.8998 | 0.9755 | 0.9163 | 0.9351 | 0.8927 | 0.9022 | 0.8941 | 0.9723 | 0.8509 | 0.9256 | 0.9332 | 0.9738 | 0.953 | 0.949 |
None | 0.0032 | 0 | 0.0001 | 0.0001 | 0.0011 | 0 | 0.0004 | 0 | 0.003 | 0.0003 | 0.0002 | 0 | 0.0011 | 0.0001 | 0.0013 | 0 | 0 | 0.0002 |
Nuclear Periphery | 0.0019 | 0.0001 | 0 | 0 | 0.003 | 0 | 0.0006 | 0.0001 | 0.0012 | 0.0115 | 0.0002 | 0.0001 | 0.0038 | 0.0011 | 0.0003 | 0 | 0 | 0.0003 |
Nucleolus | 0.0009 | 0.0001 | 0.0003 | 0.0045 | 0.0039 | 0 | 0.0007 | 0.0005 | 0.003 | 0.0002 | 0.0001 | 0 | 0.0012 | 0.0009 | 0.0014 | 0.0001 | 0 | 0.003 |
Nucleus | 0.0006 | 0 | 0.0001 | 0.0008 | 0.0015 | 0 | 0.0003 | 0.0001 | 0.0008 | 0.001 | 0.0001 | 0 | 0.0008 | 0.0006 | 0.0003 | 0 | 0.0001 | 0.0042 |
Peroxisomes | 0.0334 | 0.0171 | 0.0037 | 0.0127 | 0.0129 | 0.004 | 0.0175 | 0.0132 | 0.0137 | 0.0027 | 0.0127 | 0.008 | 0.0156 | 0.0058 | 0.0114 | 0.0089 | 0.0039 | 0.0037 |
Punctate Nuclear | 0.0029 | 0 | 0.0005 | 0.0001 | 0.0028 | 0 | 0.0015 | 0 | 0.0008 | 0.0004 | 0.0002 | 0 | 0.0017 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0002 |
Vacuole | 0.0013 | 0.0009 | 0.0006 | 0.016 | 0.0011 | 0.0002 | 0.0016 | 0.0015 | 0.0035 | 0.0014 | 0.0012 | 0.0004 | 0.0032 | 0.0029 | 0.0006 | 0.0002 | 0.0004 | 0.002 |
Vacuole Periphery | 0.0053 | 0.0083 | 0.0013 | 0.0057 | 0.0027 | 0.0017 | 0.0028 | 0.003 | 0.0025 | 0.0035 | 0.0019 | 0.0029 | 0.0069 | 0.0047 | 0.0012 | 0.0004 | 0.0005 | 0.0034 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.4835 | 19.656 | 15.9116 | 20.0687 | 23.3252 | 14.9836 | 23.036 | 23.1692 | 19.4143 | 24.177 |
Translational Efficiency | 0.7686 | 1.2241 | 1.0948 | 0.8734 | 0.8482 | 2.0222 | 1.4335 | 1.2339 | 1.6761 | 1.4888 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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