Standard name | |
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Human Ortholog | |
Description | Adaptor protein involved in vesicle-mediated vacuolar protein sorting; multivalent adaptor protein; facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 6 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 10 | 10 | 6 | 1 | 4 | 4 | 0 | 3 | 6 | 0 | 4 | 5 |
Bud | 9 | 13 | 11 | 7 | 21 | 50 | 52 | 84 | 73 | 72 | 78 | 73 | 8 | 8 | 4 | 0 | 4 | 4 | 0 | 1 | 1 | 1 | 1 | 1 |
Bud Neck | 0 | 1 | 16 | 4 | 1 | 0 | 6 | 9 | 4 | 3 | 5 | 1 | 18 | 18 | 9 | 0 | 2 | 0 | 1 | 4 | 1 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 3 | 0 | 0 | 5 | 6 | 13 | 13 | 23 | 28 | 24 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 20 | 20 | 39 | 21 | 25 | 38 | 36 | 47 | 32 | 60 | 40 | 25 | 54 | 54 | 65 | 49 | 66 | 48 | 5 | 2 | 2 | 2 | 10 | 4 |
Endoplasmic Reticulum | 0 | 3 | 2 | 2 | 1 | 3 | 0 | 2 | 0 | 0 | 1 | 0 | 10 | 5 | 3 | 2 | 12 | 11 | 0 | 0 | 1 | 2 | 4 | 2 |
Endosome | 307 | 185 | 211 | 108 | 134 | 72 | 48 | 26 | 13 | 6 | 6 | 27 | 209 | 206 | 143 | 68 | 92 | 79 | 455 | 355 | 431 | 275 | 444 | 425 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 3 | 2 | 5 | 6 |
Mitochondria | 60 | 84 | 16 | 53 | 51 | 276 | 358 | 286 | 323 | 457 | 463 | 393 | 1 | 1 | 0 | 16 | 15 | 6 | 9 | 17 | 10 | 11 | 34 | 32 |
Nucleus | 2 | 2 | 0 | 2 | 0 | 3 | 3 | 4 | 1 | 2 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 3 | 2 | 0 | 1 | 3 | 1 | 0 | 1 | 2 | 1 | 3 | 2 | 2 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
Nucleolus | 25 | 21 | 8 | 5 | 4 | 16 | 19 | 16 | 12 | 18 | 16 | 22 | 4 | 4 | 5 | 0 | 0 | 1 | 2 | 4 | 3 | 1 | 3 | 1 |
Peroxisomes | 80 | 10 | 54 | 66 | 68 | 153 | 124 | 111 | 83 | 99 | 113 | 175 | 2 | 3 | 41 | 2 | 1 | 1 | 11 | 14 | 36 | 12 | 16 | 18 |
SpindlePole | 229 | 126 | 380 | 159 | 163 | 249 | 270 | 308 | 191 | 215 | 253 | 290 | 249 | 260 | 196 | 37 | 71 | 63 | 55 | 40 | 92 | 43 | 34 | 23 |
Vac/Vac Membrane | 21 | 24 | 4 | 4 | 3 | 5 | 4 | 2 | 1 | 0 | 1 | 3 | 9 | 6 | 16 | 6 | 14 | 9 | 4 | 12 | 3 | 5 | 21 | 12 |
Unique Cell Count | 487 | 369 | 540 | 294 | 331 | 582 | 652 | 622 | 518 | 666 | 702 | 685 | 460 | 461 | 377 | 146 | 206 | 171 | 555 | 471 | 603 | 363 | 591 | 544 |
Labelled Cell Count | 757 | 498 | 750 | 431 | 473 | 873 | 928 | 908 | 748 | 957 | 1007 | 1037 | 576 | 577 | 492 | 183 | 282 | 226 | 555 | 471 | 603 | 363 | 591 | 544 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 4.9 | 4.4 | 4.0 | 3.8 | 3.1 | 3.0 | 3.3 | 3.0 | 2.7 | 2.8 | 2.9 | 5.8 | 5.8 | 5.8 | 5.9 | 6.3 | 6.6 | 5.7 | 5.9 | 6.0 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.8 | 1.2 | 1.2 | 0.8 | 1.1 | 1.0 | 0.9 | 0.9 | 0.8 | 0.6 | 0.9 | 0.9 | 1.3 | 1.7 | 1.8 | 2.0 | 1.3 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.2 | -0.47 | -0.53 | -0.41 | -0.52 | -0.69 | -0.66 | -0.6 | 0.42 | 0.42 | 0.41 | 0.43 | 0.53 | 0.6 | 0.38 | 0.43 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.6 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.3 | -0.3 | -1.1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0.8 | -0.5 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.2 | -0.5 | -1.2 | 0.2 | -0.7 | 1.1 | -1.1 | -2.8 | 2.5 | 2.4 | 4.7 | 8.4 | 8.7 | 7.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 1.8 | -0.3 | 1.6 | 1.4 | 1.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.3 | -3.4 | -3.4 | 4.1 | 2.6 | 0.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.9 | -0.9 | -0.2 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.6 | -6.4 | 0.4 | -3.4 | -4.4 | -4.0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.3 | -4.6 | -5.7 | -9.9 | -9.0 | -7.9 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 3.6 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | 0.0073 | 0.0006 | 0.0009 | 0.0008 | 0.0008 | 0.0025 | 0.001 | 0.0014 | 0.0009 | 0.0001 | 0.0019 | 0.0003 |
Bud | – | – | – | – | – | – | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.001 | 0.0033 | 0.0009 | 0.0002 | 0.0013 |
Bud Neck | – | – | – | – | – | – | 0.0006 | 0.0007 | 0.0009 | 0.0003 | 0.0002 | 0.0007 | 0.0005 | 0.0005 | 0.0008 | 0.0003 | 0.0002 | 0.0007 |
Bud Periphery | – | – | – | – | – | – | 0.0003 | 0.0003 | 0.0007 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0004 | 0.0018 | 0.0001 | 0.0001 | 0.0008 |
Bud Site | – | – | – | – | – | – | 0.0007 | 0.0015 | 0.001 | 0.0002 | 0.0001 | 0.0002 | 0.0021 | 0.0046 | 0.0021 | 0.0006 | 0.0001 | 0.0014 |
Cell Periphery | – | – | – | – | – | – | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 |
Cytoplasm | – | – | – | – | – | – | 0.0051 | 0.0223 | 0.0048 | 0.002 | 0.0039 | 0.0129 | 0.0114 | 0.0123 | 0.0086 | 0.0039 | 0.0061 | 0.0114 |
Cytoplasmic Foci | – | – | – | – | – | – | 0.2559 | 0.2791 | 0.2746 | 0.2139 | 0.2788 | 0.2934 | 0.2637 | 0.2882 | 0.2744 | 0.2919 | 0.3151 | 0.2847 |
Eisosomes | – | – | – | – | – | – | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | – | – | – | – | – | – | 0.0008 | 0.0013 | 0.0003 | 0.0002 | 0.0001 | 0.001 | 0.0011 | 0.0006 | 0.0004 | 0.0001 | 0.0003 | 0.0005 |
Endosome | – | – | – | – | – | – | 0.2739 | 0.257 | 0.2366 | 0.242 | 0.2379 | 0.1996 | 0.2518 | 0.3248 | 0.2369 | 0.2169 | 0.2043 | 0.2086 |
Golgi | – | – | – | – | – | – | 0.0292 | 0.0299 | 0.034 | 0.0349 | 0.0306 | 0.0249 | 0.0247 | 0.0277 | 0.0311 | 0.0289 | 0.033 | 0.0232 |
Lipid Particles | – | – | – | – | – | – | 0.2242 | 0.1327 | 0.1692 | 0.2268 | 0.1373 | 0.2359 | 0.2007 | 0.1293 | 0.1641 | 0.1785 | 0.1308 | 0.2048 |
Mitochondria | – | – | – | – | – | – | 0.0059 | 0.011 | 0.005 | 0.0384 | 0.005 | 0.0032 | 0.0038 | 0.004 | 0.0065 | 0.0067 | 0.0129 | 0.0023 |
None | – | – | – | – | – | – | 0.01 | 0.0573 | 0.0429 | 0.0313 | 0.0219 | 0.036 | 0.0429 | 0.03 | 0.0451 | 0.0441 | 0.0415 | 0.0427 |
Nuclear Periphery | – | – | – | – | – | – | 0.0009 | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0016 | 0.0009 | 0.0007 | 0.0006 | 0.0001 | 0.0003 | 0.0006 |
Nucleolus | – | – | – | – | – | – | 0.0004 | 0.0012 | 0.0005 | 0.0001 | 0.0002 | 0.0008 | 0.0009 | 0.0022 | 0.0013 | 0.006 | 0.0003 | 0.0005 |
Nucleus | – | – | – | – | – | – | 0.0004 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0008 | 0.0009 | 0.0016 | 0.0008 | 0.001 | 0.0002 | 0.0005 |
Peroxisomes | – | – | – | – | – | – | 0.1754 | 0.1862 | 0.2168 | 0.2054 | 0.2575 | 0.1663 | 0.1795 | 0.1559 | 0.2076 | 0.2143 | 0.2454 | 0.2019 |
Punctate Nuclear | – | – | – | – | – | – | 0.0038 | 0.0071 | 0.0041 | 0.0013 | 0.0236 | 0.0161 | 0.007 | 0.005 | 0.0084 | 0.002 | 0.0047 | 0.0093 |
Vacuole | – | – | – | – | – | – | 0.0041 | 0.0089 | 0.0054 | 0.0012 | 0.0012 | 0.0033 | 0.0048 | 0.0081 | 0.004 | 0.0026 | 0.0016 | 0.0034 |
Vacuole Periphery | – | – | – | – | – | – | 0.0008 | 0.0011 | 0.0007 | 0.0006 | 0.0004 | 0.0007 | 0.0009 | 0.0014 | 0.0009 | 0.0007 | 0.001 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.4151 | 6.3719 | 6.6196 | 4.5467 | 4.1588 | 6.4116 | 6.6037 | 7.3922 | 5.6745 | 6.6696 |
Translational Efficiency | 0.5999 | 0.7213 | 0.5032 | 0.5418 | 0.8924 | 0.5702 | 0.9295 | 0.7344 | 0.9734 | 0.8069 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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