Standard name
Human Ortholog
Description Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.98 0.91 0.89 0.87 0.78 0.72 – 0.7 0.61 0.65 0.61 0.88 0.91 0.94 0.92 0.9 0.9 0.83 0.77 0.85 0.84 0.87 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0 – 0.05 0.05 0.05 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0.06 0 0 0 0 – 0 0.05 0.05 0.05 0 0 0 0.05 0.05 0.07 0.09 0.14 0.07 0.05 0.05 0.06
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0 0.06 0.15 0.15 0.18 0.25 – 0.2 0.34 0.22 0.19 0.15 0.15 0.07 0.09 0.17 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 1 2 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 1 0 – 0 2 2 1 0 0 0 0 0 1 1 2 0 5 0 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 2 0 3 – 3 0 1 6 0 0 0 1 4 1 0 0 0 0 0 0
Cytoplasm 132 195 97 147 189 87 154 – 155 106 75 124 148 354 356 172 180 165 135 156 117 168 313 265
Endoplasmic Reticulum 1 2 1 1 6 2 6 – 7 8 1 5 6 4 2 4 2 5 0 0 0 1 3 6
Endosome 0 0 0 0 0 0 1 – 0 0 1 0 0 0 0 0 0 1 1 2 0 0 2 0
Golgi 1 0 0 0 2 0 0 – 0 1 1 1 0 0 0 0 0 0 2 0 0 2 0 0
Mitochondria 0 0 0 0 0 5 0 – 11 9 6 28 0 0 1 1 1 1 0 0 1 0 1 2
Nucleus 2 4 6 5 7 1 4 – 7 9 6 11 1 0 0 9 10 12 15 28 9 10 18 17
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 0 0 0 3 1 0
Nucleolus 0 0 0 1 0 1 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Vac/Vac Membrane 25 4 6 25 32 20 53 – 44 59 26 38 25 60 28 16 33 24 5 8 4 4 7 6
Unique Cell Count 149 198 107 166 217 111 213 220 175 116 203 168 390 377 186 200 184 164 203 138 202 360 309
Labelled Cell Count 161 206 111 179 238 117 221 227 194 120 219 180 418 387 203 230 210 164 203 138 202 360 309


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.9 13.0 12.0 12.4 14.7 9.2 12.2 – 10.3 10.4 9.7 9.4 15.7 16.1 16.0 15.2 16.6 16.8 12.1 12.9 12.4
Std Deviation (1e-4) 2.1 2.1 1.7 2.1 2.4 2.4 2.1 – 1.7 1.8 1.8 1.7 2.9 3.0 3.0 3.5 4.3 3.5 2.2 2.3 2.2
Intensity Change (Log2) – – – 0.04 0.29 -0.39 0.02 – -0.22 -0.2 -0.31 -0.36 0.39 0.42 0.41 0.34 0.47 0.49 0.01 0.1 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -0.9 -2.5 -3.8 – -4.1 -5.5 -4.6 -5.5 -0.7 0 1.4 0.5 -0.2 -0.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 4.0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 -0.2 -0.1 -0.1 0 0 0 -0.3 -0.2 0.3
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 2.4 2.4 2.8 4.2 – 3.4 5.4 3.6 3.1 2.4 2.6 0.6 0.9 2.7 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.0011 9.1773 8.2517 7.4055 6.337 7.8063 10.1454 13.2618 11.3967 11.9298 8.4014 11.056 10.2018 13.5134 11.1357 10.5866 10.0056 11.7687
Actin 0.0331 0.0007 0.0121 0.001 0.0249 0.005 0.0087 0.0003 0.0313 0.0002 0.0527 0.0002 0.0192 0.0005 0.0025 0.0003 0.1552 0.0002
Bud 0.0006 0.0002 0.0007 0.0001 0.0025 0.0003 0.0005 0.0001 0.0006 0.0001 0.0018 0.0001 0.0006 0.0001 0.0002 0.0001 0.0012 0.0001
Bud Neck 0.0054 0.0004 0.001 0.0005 0.0097 0.0018 0.0046 0.0003 0.0009 0.0005 0.0023 0.0012 0.0009 0.0005 0.0006 0.0007 0.0013 0.0011
Bud Periphery 0.0007 0 0.001 0 0.0019 0.0003 0.0006 0 0.0007 0 0.0026 0 0.0005 0 0.0001 0 0.0021 0
Bud Site 0.0045 0.0025 0.004 0 0.0049 0.0002 0.0008 0.0002 0.0073 0.0001 0.0033 0 0.0006 0.0003 0.0009 0.0001 0.0023 0
Cell Periphery 0.0001 0.0001 0.0001 0 0.0005 0 0.0001 0 0.0001 0 0.0006 0 0.0001 0 0.0002 0 0.0004 0
Cytoplasm 0.5625 0.8074 0.734 0.7245 0.6271 0.6618 0.7084 0.8429 0.7869 0.7592 0.6012 0.7527 0.769 0.8889 0.8163 0.8159 0.5928 0.7796
Cytoplasmic Foci 0.0091 0.0014 0.0043 0.0014 0.0169 0.0031 0.0076 0.0005 0.0044 0.0006 0.0087 0.0006 0.0016 0.0007 0.0105 0.0012 0.0063 0.0011
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.0008 0 0.0001 0 0.0001 0 0.001 0
Endoplasmic Reticulum 0.0055 0.0016 0.0021 0.0006 0.0031 0.0043 0.0045 0.0006 0.0012 0.0009 0.0038 0.0016 0.0043 0.0009 0.0016 0.0008 0.0173 0.0013
Endosome 0.0223 0.0007 0.0145 0.0005 0.0595 0.0236 0.0103 0.0003 0.0023 0.0006 0.0284 0.0004 0.0046 0.0002 0.0071 0.0004 0.0646 0.0008
Golgi 0.0112 0.0001 0.0049 0 0.0115 0.0122 0.0016 0 0.0017 0 0.0178 0 0.0017 0 0.0022 0 0.032 0
Lipid Particles 0.0043 0 0.0069 0 0.0148 0.0061 0.0068 0 0.0008 0 0.0182 0 0.0004 0 0.0059 0 0.0107 0
Mitochondria 0.0136 0.0002 0.0059 0.0002 0.0388 0.0056 0.0017 0.0002 0.0015 0.0002 0.072 0.0002 0.0021 0.0001 0.0012 0.0002 0.0108 0.0002
None 0.0035 0.0009 0.0011 0.0011 0.0038 0.0009 0.0033 0.0004 0.0008 0.0005 0.0019 0.0006 0.0026 0.0012 0.0319 0.0008 0.0035 0.0012
Nuclear Periphery 0.0365 0.0086 0.0126 0.0082 0.0104 0.0257 0.0281 0.007 0.0078 0.0118 0.0115 0.0092 0.0145 0.0042 0.0053 0.0052 0.0166 0.0097
Nucleolus 0.0007 0.0001 0.0002 0 0.0037 0.0002 0.0003 0.0001 0.0001 0.0001 0.0013 0 0.0005 0 0.0002 0.0001 0.0002 0.0001
Nucleus 0.2578 0.1727 0.1899 0.2603 0.1289 0.242 0.2006 0.1453 0.1478 0.2226 0.1393 0.2316 0.1722 0.0999 0.101 0.1708 0.073 0.2017
Peroxisomes 0.0018 0.0001 0.0008 0 0.009 0.0007 0.0016 0 0.0015 0 0.0109 0 0.0003 0 0.0087 0 0.0019 0
Punctate Nuclear 0.0226 0.0014 0.0021 0.0009 0.0053 0.0023 0.0072 0.0015 0.0016 0.0017 0.0031 0.001 0.0027 0.002 0.0029 0.0031 0.0028 0.0022
Vacuole 0.0023 0.0008 0.0013 0.0003 0.0179 0.002 0.0015 0.0003 0.0005 0.0007 0.0122 0.0003 0.0009 0.0002 0.0005 0.0002 0.0017 0.0005
Vacuole Periphery 0.0018 0.0001 0.0005 0.0001 0.0046 0.0018 0.0008 0.0001 0.0002 0.0002 0.0058 0.0001 0.0006 0 0.0002 0.0001 0.0022 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 153.7222 131.5168 112.6052 114.6363 119.829 156.4647 123.1738 123.6942 166.9742 112.7122
Translational Efficiency 1.7513 1.8823 1.9441 2.6317 2.3359 1.981 1.6487 2.3117 1.7548 2.0857

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Skp1

Skp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Skp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available