Standard name | |
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Human Ortholog | |
Description | Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 1 | 4 | – | – | 0 | 1 | 0 | – | – | – | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 |
Bud | 2 | 0 | 0 | 2 | 0 | 0 | 2 | – | – | 2 | 3 | 1 | – | – | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 2 | 4 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 1 | – | – | 1 | 1 | 1 | – | – | – | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 154 | 86 | 18 | 27 | 25 | 55 | 86 | – | – | 19 | 85 | 27 | – | – | – | 130 | 172 | 122 | 53 | 38 | 14 | 1 | 5 | 1 |
Endoplasmic Reticulum | 18 | 19 | 0 | 0 | 2 | 5 | 5 | – | – | 0 | 1 | 2 | – | – | – | 8 | 1 | 1 | 65 | 39 | 4 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 2 | 0 | – | – | – | 7 | 12 | 6 | 4 | 1 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3 | 0 | 0 | 0 | 0 | 2 | 0 | – | – | 0 | 10 | 3 | – | – | – | 0 | 2 | 0 | 6 | 2 | 2 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | 0 | 1 | 4 | – | – | – | 11 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | 0 | 2 | 0 | – | – | – | 81 | 55 | 46 | 55 | 21 | 17 | 0 | 2 | 0 |
Unique Cell Count | 174 | 101 | 19 | 27 | 26 | 62 | 94 | 20 | 95 | 33 | 206 | 222 | 163 | 199 | 116 | 45 | 4 | 12 | 5 | |||||
Labelled Cell Count | 180 | 106 | 19 | 29 | 28 | 63 | 99 | 22 | 106 | 39 | 239 | 250 | 182 | 199 | 116 | 45 | 4 | 12 | 5 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 7.0 | 7.4 | 6.6 | 7.5 | 6.1 | 6.3 | – | – | 5.4 | 4.8 | 4.9 | – | – | – | 8.9 | 9.3 | 8.6 | – | – | – |
Std Deviation (1e-4) | 0.7 | 1.2 | 2.3 | 0.7 | 0.8 | 1.0 | 1.3 | – | – | 0.5 | 0.9 | 1.4 | – | – | – | 1.5 | 1.6 | 1.5 | – | – | – |
Intensity Change (Log2) | – | – | – | -0.18 | 0.01 | -0.28 | -0.23 | – | – | -0.47 | -0.63 | -0.61 | – | – | – | 0.26 | 0.32 | 0.21 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cytoplasm | 1.2 | 0.2 | -0.8 | -0.5 | – | – | 0 | -0.7 | -1.3 | – | – | – | -2.8 | -1.8 | -1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | – | – | – | 3.0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.6247 | 2.0165 | 1.6075 | 1.6942 | 1.3942 | 1.8246 | 1.4043 | 2.0542 | 1.8228 | 1.2987 | 1.7551 | 2.0478 | 1.0935 | 1.6405 | 1.3591 | 1.6848 | 1.3012 | 1.5435 |
Actin | 0.0218 | 0.0054 | 0.0071 | 0.0079 | 0.0089 | 0.0107 | 0.0135 | 0.0016 | 0.0046 | 0.0027 | 0.0268 | 0.0159 | 0.0281 | 0.0006 | 0.0176 | 0.0066 | 0.1138 | 0.0147 |
Bud | 0.0012 | 0.0128 | 0.0024 | 0.0018 | 0.0008 | 0.0017 | 0.0006 | 0.0003 | 0.0004 | 0.0003 | 0.0014 | 0.0007 | 0.0003 | 0.0015 | 0.0019 | 0.0005 | 0.0009 | 0.0112 |
Bud Neck | 0.0065 | 0.002 | 0.0048 | 0.0034 | 0.0028 | 0.0066 | 0.0014 | 0.0008 | 0.0033 | 0.0058 | 0.002 | 0.0034 | 0.0012 | 0.0003 | 0.0014 | 0.0004 | 0.0009 | 0.0016 |
Bud Periphery | 0.0027 | 0.0028 | 0.0037 | 0.0017 | 0.0013 | 0.0017 | 0.0015 | 0.0008 | 0.0009 | 0.0012 | 0.0032 | 0.0028 | 0.0003 | 0.0006 | 0.0036 | 0.0008 | 0.0012 | 0.0039 |
Bud Site | 0.008 | 0.0311 | 0.0129 | 0.0009 | 0.0021 | 0.0009 | 0.0067 | 0.0119 | 0.0131 | 0.001 | 0.0033 | 0.001 | 0.0032 | 0.0243 | 0.0048 | 0.0008 | 0.0034 | 0.0007 |
Cell Periphery | 0.0045 | 0.0036 | 0.0033 | 0.0012 | 0.0022 | 0.0011 | 0.0051 | 0.0095 | 0.0058 | 0.0085 | 0.0019 | 0.0021 | 0.0023 | 0.003 | 0.0022 | 0.0004 | 0.0011 | 0.0009 |
Cytoplasm | 0.4717 | 0.5863 | 0.5276 | 0.6091 | 0.6218 | 0.5891 | 0.4546 | 0.5685 | 0.4875 | 0.602 | 0.5215 | 0.5575 | 0.489 | 0.616 | 0.5988 | 0.4394 | 0.5345 | 0.6661 |
Cytoplasmic Foci | 0.0264 | 0.0213 | 0.0306 | 0.0293 | 0.0407 | 0.0456 | 0.0447 | 0.0225 | 0.0287 | 0.0185 | 0.0255 | 0.0228 | 0.033 | 0.0124 | 0.0201 | 0.0629 | 0.0096 | 0.0335 |
Eisosomes | 0.0011 | 0.0006 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 |
Endoplasmic Reticulum | 0.1462 | 0.12 | 0.1092 | 0.0566 | 0.0895 | 0.0982 | 0.1513 | 0.1524 | 0.145 | 0.1512 | 0.1158 | 0.1074 | 0.2049 | 0.1561 | 0.1517 | 0.1984 | 0.233 | 0.1365 |
Endosome | 0.0544 | 0.0392 | 0.0501 | 0.0521 | 0.074 | 0.039 | 0.0568 | 0.0362 | 0.0357 | 0.0613 | 0.0804 | 0.0272 | 0.0226 | 0.0214 | 0.0186 | 0.0437 | 0.0359 | 0.0194 |
Golgi | 0.0082 | 0.0023 | 0.0115 | 0.0095 | 0.0069 | 0.0067 | 0.0136 | 0.0023 | 0.0092 | 0.0093 | 0.0133 | 0.008 | 0.0067 | 0.0011 | 0.0087 | 0.021 | 0.0212 | 0.0119 |
Lipid Particles | 0.0213 | 0.0121 | 0.0187 | 0.0166 | 0.0124 | 0.0173 | 0.0291 | 0.0235 | 0.0165 | 0.0072 | 0.0368 | 0.0087 | 0.0203 | 0.0056 | 0.0055 | 0.0504 | 0.0064 | 0.0172 |
Mitochondria | 0.0132 | 0.0009 | 0.0089 | 0.0373 | 0.004 | 0.006 | 0.0116 | 0.0009 | 0.0094 | 0.014 | 0.0109 | 0.0131 | 0.0029 | 0.0026 | 0.0061 | 0.0519 | 0.0085 | 0.0036 |
None | 0.1626 | 0.1217 | 0.1543 | 0.1168 | 0.0618 | 0.1455 | 0.1662 | 0.1364 | 0.2055 | 0.1025 | 0.0677 | 0.2044 | 0.1532 | 0.1336 | 0.1458 | 0.0842 | 0.0138 | 0.0585 |
Nuclear Periphery | 0.0125 | 0.0077 | 0.0074 | 0.0053 | 0.0071 | 0.0032 | 0.0056 | 0.0046 | 0.0038 | 0.0024 | 0.0039 | 0.0059 | 0.0039 | 0.0035 | 0.003 | 0.0049 | 0.0051 | 0.0019 |
Nucleolus | 0.0014 | 0.0003 | 0.001 | 0.0016 | 0.0019 | 0.0005 | 0.0007 | 0.0002 | 0.0013 | 0.0001 | 0.0011 | 0.0005 | 0.0003 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0034 |
Nucleus | 0.0065 | 0.0037 | 0.0062 | 0.0055 | 0.011 | 0.0036 | 0.0022 | 0.0025 | 0.0028 | 0.0015 | 0.0032 | 0.0019 | 0.0014 | 0.0023 | 0.001 | 0.0011 | 0.0011 | 0.0022 |
Peroxisomes | 0.0051 | 0.0029 | 0.0148 | 0.005 | 0.0178 | 0.007 | 0.0111 | 0.0025 | 0.0091 | 0.0023 | 0.0236 | 0.0034 | 0.0149 | 0.0001 | 0.0015 | 0.0183 | 0.0015 | 0.0027 |
Punctate Nuclear | 0.0063 | 0.0012 | 0.0072 | 0.0144 | 0.0034 | 0.0054 | 0.0035 | 0.001 | 0.0016 | 0.0006 | 0.0186 | 0.0031 | 0.0044 | 0.0008 | 0.0006 | 0.005 | 0.0009 | 0.0008 |
Vacuole | 0.0169 | 0.0208 | 0.0159 | 0.0213 | 0.0258 | 0.0092 | 0.0179 | 0.0207 | 0.0144 | 0.0067 | 0.0355 | 0.0088 | 0.0063 | 0.0129 | 0.0062 | 0.0081 | 0.0055 | 0.0081 |
Vacuole Periphery | 0.0017 | 0.0012 | 0.0015 | 0.0025 | 0.0037 | 0.0008 | 0.0017 | 0.0005 | 0.001 | 0.0007 | 0.0034 | 0.001 | 0.0004 | 0.0005 | 0.0005 | 0.0012 | 0.0011 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.6569 | 39.6533 | 34.3285 | 41.4292 | 33.5487 | 28.9399 | 44.6875 | 44.9127 | 40.8064 | 50.8998 |
Translational Efficiency | 0.5987 | 0.5822 | 0.6428 | 0.4037 | 0.4726 | 0.8397 | 0.5576 | 0.4319 | 0.4676 | 0.4159 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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