Standard name | |
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Human Ortholog | |
Description | Specific translational activator for the mitochondrial ATP6 mRNA; Atp6p encodes a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | – | 2 | 5 | 3 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 2 | 4 | 13 | 0 | – | – | – | 1 | 1 | – | 4 | 1 | 0 | 0 | 0 | 1 |
Bud Neck | 2 | 4 | 5 | 3 | 12 | 8 | 12 | 11 | 6 | 12 | 4 | 9 | – | – | – | 5 | 1 | – | 1 | 1 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | – | – | – | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 6 | 3 | 7 | 3 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | – | – | – | 4 | 1 | – | 2 | 5 | 2 | 0 | 0 | 0 |
Cytoplasm | 402 | 494 | 206 | 215 | 244 | 226 | 399 | 256 | 197 | 210 | 181 | 215 | – | – | – | 381 | 247 | – | 293 | 421 | 150 | 12 | 23 | 58 |
Endoplasmic Reticulum | 2 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 5 | 0 | 1 | 0 | – | – | – | 25 | 20 | – | 1 | 0 | 2 | 0 | 0 | 0 |
Endosome | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | – | – | – | 6 | 2 | – | 4 | 2 | 1 | 0 | 0 | 2 |
Golgi | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | – | 3 | 2 | 0 | 0 | 0 | 0 |
Mitochondria | 11 | 4 | 0 | 2 | 0 | 22 | 27 | 18 | 56 | 41 | 155 | 78 | – | – | – | 17 | 2 | – | 30 | 25 | 24 | 0 | 0 | 1 |
Nucleus | 0 | 0 | 0 | 5 | 1 | 1 | 1 | 0 | 0 | 3 | 2 | 4 | – | – | – | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 0 | 3 | 2 | 7 | 10 | 7 | 9 | 10 | 4 | – | – | – | 0 | 1 | – | 3 | 2 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 2 | 1 | 2 | – | – | – | 3 | 3 | – | 3 | 4 | 5 | 0 | 0 | 0 |
Unique Cell Count | 413 | 504 | 209 | 220 | 249 | 244 | 415 | 278 | 244 | 248 | 306 | 278 | 400 | 254 | 363 | 489 | 201 | 15 | 26 | 74 | ||||
Labelled Cell Count | 426 | 511 | 221 | 230 | 264 | 264 | 452 | 297 | 278 | 281 | 368 | 314 | 442 | 280 | 363 | 489 | 201 | 15 | 26 | 74 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 3.8 | 4.8 | 4.7 | 4.9 | 3.9 | 3.9 | 4.4 | 3.4 | 3.6 | 3.0 | 3.3 | – | – | – | 5.1 | 5.1 | 4.8 | – | 7.1 | 6.4 |
Std Deviation (1e-4) | 0.9 | 0.6 | 1.1 | 1.5 | 1.5 | 1.6 | 1.3 | 1.7 | 1.0 | 1.2 | 0.9 | 0.9 | – | – | – | 1.2 | 1.1 | 0.8 | – | 3.7 | 3.4 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.05 | -0.28 | -0.27 | -0.11 | -0.5 | -0.39 | -0.66 | -0.53 | – | – | – | 0.1 | 0.11 | 0.02 | – | 0.58 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1454 | -0.6125 | -0.5285 | -1.2762 | -0.2848 | -1.0118 | -0.4269 | -0.511 | -0.4842 | -0.5518 | -0.0234 | -0.4072 | -1.222 | -0.7883 | -0.8983 | -0.341 | -1.7851 | -0.8094 |
Actin | 0.0362 | 0.0015 | 0.0109 | 0.0001 | 0.0088 | 0.0004 | 0.0285 | 0.0003 | 0.009 | 0.0241 | 0.0221 | 0.0007 | 0.0286 | 0.0002 | 0.0195 | 0.0426 | 0.0006 | 0.0035 |
Bud | 0.0123 | 0.0066 | 0.0155 | 0.0001 | 0.0019 | 0.0083 | 0.0017 | 0.0009 | 0.0006 | 0.0046 | 0.0026 | 0.0002 | 0.0005 | 0.0065 | 0.0003 | 0.001 | 0 | 0.0048 |
Bud Neck | 0.0067 | 0.0003 | 0.0012 | 0.0001 | 0.0013 | 0.0034 | 0.0064 | 0.0003 | 0.0037 | 0.0055 | 0.0006 | 0.0045 | 0.001 | 0.0001 | 0.0064 | 0.0043 | 0.0085 | 0.0008 |
Bud Periphery | 0.0031 | 0.0025 | 0.0116 | 0.0001 | 0.0027 | 0.002 | 0.0088 | 0.0012 | 0.0009 | 0.0065 | 0.0122 | 0.0004 | 0.0007 | 0.0011 | 0.0003 | 0.0019 | 0 | 0.0035 |
Bud Site | 0.0132 | 0.0091 | 0.0135 | 0.0002 | 0.0122 | 0.0038 | 0.0054 | 0.0077 | 0.0093 | 0.0297 | 0.0012 | 0 | 0.0032 | 0.0026 | 0.0055 | 0.0098 | 0.0001 | 0.0004 |
Cell Periphery | 0.0005 | 0.0003 | 0.0006 | 0 | 0.0002 | 0.0003 | 0.001 | 0.0001 | 0.0002 | 0.0006 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 |
Cytoplasm | 0.012 | 0.0311 | 0.0301 | 0.0097 | 0.0352 | 0.0649 | 0.0127 | 0.0084 | 0.0062 | 0.007 | 0.0175 | 0.0079 | 0.0283 | 0.0481 | 0.0606 | 0.0638 | 0.0682 | 0.1494 |
Cytoplasmic Foci | 0.0391 | 0.009 | 0.019 | 0.0005 | 0.0121 | 0.014 | 0.0067 | 0.0029 | 0.0031 | 0.0086 | 0.0071 | 0.0026 | 0.004 | 0.0048 | 0.0037 | 0.008 | 0.0046 | 0.0066 |
Eisosomes | 0.0007 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0005 | 0.001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0024 | 0.0006 | 0.0091 | 0.0001 | 0.0038 | 0.0004 | 0.0013 | 0.0002 | 0.0001 | 0.0018 | 0.0009 | 0.0001 | 0.0025 | 0.0004 | 0.0012 | 0.0021 | 0.0044 | 0.0013 |
Endosome | 0.0235 | 0.0005 | 0.0081 | 0 | 0.0209 | 0.0036 | 0.007 | 0.0001 | 0.0008 | 0.0093 | 0.0031 | 0.0001 | 0.0084 | 0.0004 | 0.0045 | 0.008 | 0.0035 | 0.0033 |
Golgi | 0.0073 | 0.0001 | 0.0031 | 0 | 0.002 | 0.0005 | 0.0018 | 0.0001 | 0.0014 | 0.0045 | 0.0014 | 0.0001 | 0.0028 | 0 | 0.0012 | 0.0026 | 0.0001 | 0.0016 |
Lipid Particles | 0.0232 | 0.0007 | 0.003 | 0 | 0.0199 | 0.0073 | 0.0047 | 0.0002 | 0.0012 | 0.0123 | 0.0034 | 0.0001 | 0.0039 | 0.0001 | 0.0005 | 0.0075 | 0.0005 | 0.0096 |
Mitochondria | 0.0113 | 0.0011 | 0.0027 | 0 | 0.0041 | 0.0014 | 0.0011 | 0.0002 | 0.0034 | 0.003 | 0.0319 | 0.0002 | 0.0014 | 0.0001 | 0.0006 | 0.0049 | 0.0011 | 0.0035 |
None | 0.7648 | 0.9178 | 0.8438 | 0.985 | 0.809 | 0.8687 | 0.8798 | 0.9707 | 0.9435 | 0.8375 | 0.8407 | 0.9809 | 0.8932 | 0.9238 | 0.8897 | 0.8298 | 0.7329 | 0.8011 |
Nuclear Periphery | 0.0054 | 0.0032 | 0.0041 | 0.0002 | 0.0258 | 0.0005 | 0.0044 | 0.0003 | 0.0005 | 0.0053 | 0.0039 | 0.0003 | 0.0106 | 0.0004 | 0.0021 | 0.0021 | 0.0168 | 0.0029 |
Nucleolus | 0.0031 | 0.0014 | 0.0012 | 0.0005 | 0.0005 | 0.0021 | 0.0023 | 0.0004 | 0.0004 | 0.0021 | 0.0018 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0063 | 0.0004 |
Nucleus | 0.0049 | 0.004 | 0.0033 | 0.0007 | 0.0097 | 0.0041 | 0.0052 | 0.0017 | 0.0007 | 0.0076 | 0.0035 | 0.0003 | 0.0031 | 0.0091 | 0.0012 | 0.0008 | 0.145 | 0.0034 |
Peroxisomes | 0.0096 | 0.001 | 0.0052 | 0 | 0.0194 | 0.002 | 0.0142 | 0.0003 | 0.0087 | 0.0198 | 0.0333 | 0.0008 | 0.0029 | 0.0001 | 0.0004 | 0.0056 | 0.0001 | 0.0009 |
Punctate Nuclear | 0.0153 | 0.008 | 0.0113 | 0.0024 | 0.0021 | 0.011 | 0.0038 | 0.0035 | 0.0059 | 0.007 | 0.0027 | 0.0005 | 0.0031 | 0.0015 | 0.0009 | 0.0018 | 0.0052 | 0.0019 |
Vacuole | 0.0039 | 0.0005 | 0.0019 | 0.0001 | 0.0058 | 0.0009 | 0.0024 | 0.0002 | 0.0002 | 0.0019 | 0.0057 | 0.0001 | 0.0005 | 0.0002 | 0.0006 | 0.0013 | 0.0014 | 0.0007 |
Vacuole Periphery | 0.0016 | 0.0002 | 0.0007 | 0 | 0.0024 | 0.0003 | 0.0005 | 0 | 0.0001 | 0.0009 | 0.0031 | 0 | 0.0005 | 0 | 0.0003 | 0.0007 | 0.0003 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.3964 | 9.1198 | 6.0549 | 11.4326 | 6.6135 | 8.0496 | 13.2249 | 10.8093 | 11.8832 | 13.1884 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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