Standard name
Human Ortholog
Description Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.09 0.07 0.06 0.07 0.06 0 0 0 0 0.14 0.16 0.22 0.08 0.2 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.19 0.15 0.08 0.23 0.18 0.43 0.22 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Nucleus 0.94 0.92 0.95 0.85 0.81 0.74 0.76 0.84 0.71 0.74 0.64 0.71 0.8 0.82 0.73 0.8 0.69 0.77 0.84 0.82 0.79 0.79 0.7 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.07 0.13 0.1 0.21 0.19 0.29 0.24 0 0 0 0 0 0 0 0 0 0 0.09 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0.09 0.05 0.05 0.07 0 0.05 0 0.05 0.08 0.1 0.06 0 0 0 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.12 0.05 0.07 0.12 0.08 0.11 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 3 0 0 0 1 0
Bud 0 0 0 0 0 0 0 0 7 3 1 1 0 0 0 1 0 0 1 2 0 0 2 3
Bud Neck 0 0 0 0 1 0 1 5 2 2 2 1 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 1 0 4 8 7 7 14 14 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Cytoplasm 7 8 8 11 13 15 20 29 8 12 4 6 23 37 57 5 25 9 2 2 0 1 2 8
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 4 4 2 0 0 1 0 0 0
Endosome 0 2 0 1 6 5 5 1 0 4 0 1 1 1 5 2 5 1 1 3 1 0 5 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 1 3 0 0 1
Mitochondria 20 10 0 5 4 44 46 42 60 56 101 59 3 0 0 4 5 5 0 5 1 0 3 5
Nucleus 248 219 133 106 153 175 228 425 185 235 148 190 132 193 185 52 85 66 237 212 121 81 164 189
Nuclear Periphery 0 0 0 2 1 1 7 1 4 1 4 1 0 0 0 0 1 1 0 0 0 0 0 1
Nucleolus 3 1 0 2 7 17 40 53 55 59 67 64 1 5 4 1 1 2 12 5 1 4 21 30
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 2 1 7 17 11 15 34 9 17 2 14 13 23 15 2 1 3 3 5 1 4 5 8
Vac/Vac Membrane 6 8 1 1 4 3 3 4 3 1 1 0 6 1 6 3 10 10 15 16 17 8 26 34
Unique Cell Count 265 239 140 124 189 236 301 507 260 317 233 267 165 236 254 65 124 86 283 260 154 103 236 295
Labelled Cell Count 287 251 143 136 207 275 373 601 342 405 344 342 179 260 274 76 137 101 283 260 154 103 236 295


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.4 6.4 6.2 6.5 5.5 5.5 5.8 5.1 5.4 4.7 5.3 6.2 6.1 6.0 6.5 7.0 6.6 6.7 6.3 6.7
Std Deviation (1e-4) 0.8 0.8 1.7 1.8 1.6 1.9 1.6 1.5 1.3 1.3 1.1 1.2 1.2 1.3 1.5 1.4 1.8 1.6 2.2 1.9 2.1
Intensity Change (Log2) – – – -0.04 0.03 -0.22 -0.21 -0.12 -0.33 -0.24 -0.44 -0.27 -0.03 -0.06 -0.09 0.03 0.14 0.05 0.08 -0.01 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.4 0.3 0.4 0 -1.3 -0.9 0 0 2.4 2.9 4.3 0 3.5 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.4 4.9 3.5 6.2 5.3 0 6.0 0 0 0 0 0 0
Nucleus -2.6 -3.7 -5.1 -4.9 -3.4 -5.6 -5.2 -6.8 -5.6 -3.9 -3.6 -5.3 -3.4 -5.7 -4.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.2 4.5 4.0 5.9 5.5 0 6.3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3.3 0 2.2 2.8 0 2.4 0 2.3 3.0 3.5 2.5 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9517 0.2401 0.3441 0.5703 -0.2214 0.4748 0.3518 0.52 0.2993 0.269 0.4339 0.4062 1.1159 0.3282 0.3972 0.8834 0.9082 0.1223
Actin 0.0552 0.0089 0.0189 0.0009 0.0011 0.0155 0.0076 0 0.0122 0.0009 0.0061 0.0072 0.0217 0 0.0218 0.0142 0.0502 0
Bud 0.002 0.0003 0.0004 0.0004 0.0002 0.0004 0.0006 0 0.0002 0.0026 0.0006 0.0006 0.001 0.0001 0.0004 0.0019 0.0005 0
Bud Neck 0.0026 0.0004 0.0005 0.0003 0.0011 0.0018 0.005 0.0002 0.0003 0.0002 0.0008 0.0016 0.0033 0.0003 0.0006 0.0003 0.0034 0.0018
Bud Periphery 0.0025 0.0002 0.0006 0.0026 0.0004 0.0005 0.0014 0 0.0007 0.004 0.0013 0.0004 0.0013 0.0001 0.0006 0.0075 0.001 0.0001
Bud Site 0.0176 0.0011 0.0041 0.0007 0.001 0.0004 0.0036 0.0001 0.0003 0.0017 0.001 0.0012 0.0137 0.0007 0.0025 0.0011 0.0041 0.0001
Cell Periphery 0.0005 0.0001 0.0003 0.0004 0.0002 0.0001 0.0003 0 0 0.001 0.0001 0.0001 0.0004 0 0.0002 0.0002 0.0004 0
Cytoplasm 0.0057 0.0101 0.0026 0.0003 0.0058 0.0105 0.0046 0.0027 0.0009 0.0207 0.0018 0.0018 0.0144 0.0018 0.0014 0.0044 0.0027 0.0001
Cytoplasmic Foci 0.0354 0.0041 0.0069 0.0001 0.0012 0.0059 0.0152 0 0.0036 0.0078 0.0059 0.0015 0.027 0.0016 0.0046 0.0083 0.0375 0
Eisosomes 0.0006 0 0.0001 0.0003 0.0001 0.0001 0.0003 0 0.0001 0.0003 0.0001 0.0002 0.0002 0 0.0002 0.0002 0.0005 0
Endoplasmic Reticulum 0.0142 0.0018 0.0016 0.0002 0.029 0.0054 0.0012 0.0002 0.0004 0.0011 0.003 0.0006 0.0051 0.0002 0.001 0.004 0.0026 0
Endosome 0.0546 0.0013 0.0099 0.0013 0.0322 0.0331 0.0169 0.0002 0.0089 0.0017 0.0449 0.0039 0.0422 0.0038 0.0053 0.0405 0.0322 0
Golgi 0.0224 0.0009 0.0132 0.0006 0.001 0.0216 0.0045 0 0.0036 0.0002 0.0106 0.0012 0.0197 0.0001 0.0067 0.024 0.0313 0
Lipid Particles 0.027 0.001 0.0126 0.0014 0.0036 0.0039 0.0301 0 0.0054 0.0048 0.0504 0.0023 0.0231 0.0003 0.0093 0.0038 0.0519 0
Mitochondria 0.0102 0.0005 0.0097 0.1046 0.0046 0.0512 0.0078 0.0001 0.0087 0.0143 0.0117 0.0009 0.0081 0.0003 0.0081 0.0122 0.0132 0.0159
None 0.0084 0.0107 0.0028 0.0004 0.0022 0.0007 0.02 0.0016 0.0334 0.0953 0.0018 0.0056 0.0021 0.0068 0.0009 0.0009 0.0028 0
Nuclear Periphery 0.0536 0.0121 0.0069 0.0027 0.0972 0.0113 0.0099 0.0062 0.0034 0.0015 0.0086 0.0041 0.0136 0.0022 0.0024 0.0099 0.0163 0.0005
Nucleolus 0.0257 0.028 0.0459 0.023 0.1492 0.0419 0.0206 0.0216 0.0209 0.0123 0.0932 0.0339 0.0158 0.0307 0.0327 0.0148 0.098 0.0452
Nucleus 0.5959 0.8908 0.8144 0.8556 0.6198 0.7886 0.8033 0.9518 0.8811 0.8177 0.6605 0.9127 0.7453 0.9407 0.8874 0.839 0.6007 0.9343
Peroxisomes 0.02 0.0124 0.0134 0.0006 0.0003 0.0016 0.0117 0 0.0104 0.0026 0.0147 0.001 0.0056 0 0.0031 0.0015 0.0202 0
Punctate Nuclear 0.0375 0.0147 0.0292 0.001 0.0348 0.0034 0.0315 0.015 0.0049 0.003 0.0728 0.0185 0.0294 0.0084 0.0098 0.0074 0.0269 0.0017
Vacuole 0.0052 0.0005 0.0037 0.0014 0.0089 0.001 0.0028 0.0002 0.0003 0.0048 0.0072 0.0004 0.0041 0.0016 0.0008 0.0015 0.0022 0.0001
Vacuole Periphery 0.0032 0.0002 0.0021 0.0012 0.006 0.0013 0.0013 0.0001 0.0005 0.0016 0.003 0.0002 0.003 0.0003 0.0004 0.0023 0.0014 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.657 14.6564 12.3258 10.3599 14.5258 12.5628 12.8432 12.0023 11.0509 15.806
Translational Efficiency 1.4681 0.7683 0.6629 0.992 0.8075 1.0652 0.9413 0.7405 0.8715 0.7063

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Tfc6

Tfc6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tfc6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available