Standard name
Human Ortholog
Description 19S proteasome regulatory particle lid subcomplex component; role in Ub-dependent proteolysis and proteasome stability; involved in TREX-2 mediated mRNA export, and in the prevention of transcription-associated genome instability; ubiquitinated by Nedd4-like E3-ligase, Rsp5p; human ortholog DSS1, a BRCA1 binding protein implicated in cancer, complements the yeast null; drives trinucleotide repeat expansion; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 – 0 0.05 0.06 0.05 0 – – 0.25 0.18 0.14 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0.08 0 0.09 0.09 – – 0 0.06 0.06 0 0 0 0 0 0 0
Nucleus 0.91 0.88 0.84 0.81 0.48 0.53 – 0.39 0.52 0.48 0.53 0.61 – – 0.5 0.47 0.42 0.41 0.84 0.87 0.74 0.74 0.64 0.49
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.08 0.07 0.12 0.14 – 0.21 0.15 0.23 0.21 0.2 – – 0 0.05 0 0.11 0.06 0.07 0.12 0.15 0.13 0.13
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.08 0.09 0.1 0.37 0.28 – 0.34 0.22 0.25 0.19 0.12 – – 0.25 0.26 0.4 0.39 0.08 0 0.08 0.07 0.2 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 1 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 – 2 3 2 0 2 – – 0 0 0 0 1 0 7 0 0 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 0 3 – 0 5 2 3 8 – – 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 1 0 0 1 0 – – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 4 4 11 16 11 – 13 19 18 14 10 – – 4 12 7 15 0 0 1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 2 – 1 1 0 2 0 – – 0 4 1 1 0 0 1 0 0 0
Endosome 0 0 0 1 1 1 – 1 2 0 0 0 – – 0 0 0 1 0 0 1 0 0 0
Golgi 1 0 0 0 2 1 – 0 1 1 0 0 – – 0 2 1 4 0 0 0 0 0 0
Mitochondria 0 0 2 3 0 9 – 11 29 9 24 26 – – 0 4 3 1 0 0 1 0 0 0
Nucleus 292 148 303 212 122 158 – 133 186 144 147 166 – – 8 31 21 40 310 168 320 32 38 29
Nuclear Periphery 0 0 2 6 0 2 – 2 0 3 0 3 – – 0 0 1 0 1 0 4 0 0 0
Nucleolus 7 5 29 18 29 41 – 72 53 69 57 55 – – 0 3 2 11 24 14 52 6 7 7
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 1 1 0 0 0 0
Vac/Vac Membrane 22 14 34 26 93 83 – 113 80 74 52 34 – – 4 17 20 38 29 7 34 3 11 19
Unique Cell Count 321 168 360 261 252 300 337 358 300 277 274 16 66 50 98 371 194 430 44 60 61
Labelled Cell Count 328 171 375 277 263 311 350 379 322 300 304 16 73 56 111 371 194 430 44 60 61


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.7 38.8 19.5 25.3 26.3 24.4 – 23.3 22.8 22.1 21.3 20.6 – – 37.7 35.3 36.9 32.1 – 34.1 –
Std Deviation (1e-4) 7.8 8.7 5.0 6.3 6.8 7.0 – 6.2 6.6 6.0 6.4 6.4 – – 10.7 16.0 15.7 15.6 – 8.2 –
Intensity Change (Log2) – – – 0.37 0.44 0.32 – 0.26 0.23 0.18 0.13 0.08 – – 0.95 0.86 0.92 0.72 – 0.81 –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Cytoplasm 2.5 3.6 2.2 – 2.3 3.2 3.5 3.0 2.2 – – 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Mitochondria 0 0 2.4 – 2.6 5.0 2.4 5.1 5.4 – – 0 0 0 0
Nucleus -1.0 -9.5 -8.8 – -12.2 -9.3 -9.9 -8.5 -6.7 – – -3.5 -6.8 -6.9 -8.8
Nuclear Periphery 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Nucleolus -0.5 1.4 2.3 – 5.0 2.8 5.4 4.6 4.4 – – 0 0 0 1.0
Peroxisomes 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Vacuole 0.2 8.2 6.1 – 7.8 4.7 5.3 3.4 1.2 – – 0 3.8 6.0 7.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.2665 27.5901 22.8201 23.8446 22.037 24.7635 17.6804 23.9604 21.4641 18.87 18.1937 22.2279 25.8952 29.4402 27.0104 25.9061 25.6399 27.3658
Actin 0.0319 0 0.0016 0.0089 0.0058 0.0008 0.0144 0.0002 0.0078 0.0003 0.0272 0.0009 0.0385 0.0004 0 0.0022 0.0032 0.0005
Bud 0.001 0 0.0002 0.0007 0.0002 0.0001 0.0007 0 0.0005 0.0003 0.0005 0.0001 0.0009 0 0.0001 0.0003 0.0001 0.0001
Bud Neck 0.0008 0.0001 0.0001 0.0003 0.0002 0.0007 0.0042 0.0001 0.0003 0.0001 0.0005 0.0009 0.0018 0 0 0.0002 0.0008 0.001
Bud Periphery 0.0017 0 0.0002 0.0013 0.0003 0.0002 0.0012 0 0.0005 0.0013 0.0008 0.0003 0.0011 0 0.0002 0.0006 0.0002 0.0002
Bud Site 0.0023 0 0.0008 0.001 0.0004 0.0003 0.0067 0 0.0036 0.0001 0.0009 0.0001 0.0078 0.0001 0.0001 0.0003 0.0001 0.0001
Cell Periphery 0.0008 0 0.0004 0.0001 0.0001 0.0001 0.0011 0 0.0001 0.0019 0.0004 0.0001 0.0003 0 0.0002 0.0004 0.0001 0.0001
Cytoplasm 0.0075 0.0003 0.0022 0.0006 0.0013 0.0003 0.0161 0.0003 0.0054 0.0002 0.0056 0.0048 0.0123 0.0024 0.0002 0.0013 0.0002 0.0001
Cytoplasmic Foci 0.0441 0 0.0166 0.0018 0.0123 0.0026 0.0221 0.0002 0.0112 0 0.0207 0.0017 0.0196 0.0012 0 0.0086 0.0036 0.0004
Eisosomes 0.0004 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0006 0 0.0003 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0059 0 0.0001 0.0013 0.0029 0.0001 0.007 0 0.0015 0.0005 0.0047 0.0004 0.0043 0.0003 0 0.0004 0.0003 0
Endosome 0.0327 0 0.002 0.0211 0.0196 0.005 0.0168 0.0001 0.0123 0.0003 0.03 0.0046 0.029 0.0017 0 0.0048 0.0015 0.0003
Golgi 0.0096 0 0.0007 0.0103 0.0075 0.0018 0.0037 0 0.0053 0 0.0233 0.0024 0.0131 0.0002 0 0.0027 0.0032 0.0006
Lipid Particles 0.0346 0 0.0029 0.003 0.0197 0.0058 0.0074 0.0003 0.0047 0.0001 0.0252 0.0033 0.0154 0.0002 0 0.0102 0.0287 0.0032
Mitochondria 0.0144 0.0003 0.0047 0.0044 0.0137 0.0014 0.0044 0.0006 0.0027 0.0259 0.0233 0.0018 0.0045 0.0005 0.0008 0.0074 0.001 0.0013
None 0.0049 0 0.0024 0.0002 0.002 0.0001 0.01 0.0001 0.0072 0.0001 0.0147 0.0002 0.0026 0.0004 0 0.001 0.0003 0
Nuclear Periphery 0.0239 0.0074 0.0083 0.0034 0.0302 0.007 0.0272 0.0126 0.0174 0.0524 0.0244 0.0116 0.0081 0.0041 0.0056 0.0053 0.0551 0.0077
Nucleolus 0.0352 0.0051 0.0408 0.0019 0.0352 0.0344 0.0399 0.0078 0.0076 0.0074 0.0492 0.0242 0.0065 0.0074 0.0104 0.0089 0.0197 0.0289
Nucleus 0.7049 0.9864 0.9057 0.9364 0.8365 0.9349 0.7764 0.9741 0.9009 0.8983 0.7309 0.9304 0.8213 0.9804 0.9793 0.9265 0.8545 0.9517
Peroxisomes 0.0099 0 0.0024 0.0005 0.0025 0.001 0.0113 0.0001 0.0046 0.0001 0.0032 0.0064 0.0061 0.0001 0 0.0099 0.0006 0.0019
Punctate Nuclear 0.0217 0.0001 0.0017 0.0003 0.0037 0.0008 0.0136 0.0032 0.0029 0.0001 0.0065 0.0009 0.0032 0.0003 0.0001 0.004 0.0243 0.0002
Vacuole 0.0057 0.0001 0.0021 0.0014 0.0011 0.0013 0.007 0.0001 0.0014 0.0029 0.0023 0.0021 0.0019 0.0001 0.0015 0.0017 0.0004 0.0006
Vacuole Periphery 0.0062 0.0002 0.0038 0.0009 0.0049 0.001 0.0086 0.0003 0.0018 0.0076 0.0052 0.0026 0.0014 0.0002 0.0016 0.0032 0.0021 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 96.0371 138.8233 165.3628 133.2763 177.5019 142.9547 125.5889 160.8024 176.9846 145.1353
Translational Efficiency 4.4013 2.4302 2.4844 3.7586 2.1589 3.2239 3.2209 3.1789 2.8429 2.9233

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description 19S proteasome regulatory particle lid subcomplex component; role in Ub-dependent proteolysis and proteasome stability; involved in TREX-2 mediated mRNA export, and in the prevention of transcription-associated genome instability; ubiquitinated by Nedd4-like E3-ligase, Rsp5p; human ortholog DSS1, a BRCA1 binding protein implicated in cancer, complements the yeast null; drives trinucleotide repeat expansion; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Sem1

Sem1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sem1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available