Standard name
Human Ortholog
Description NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication; human homolog AKR1B1 can complement yeast null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.98 0.96 0.89 0.83 0.72 0.73 0.69 0.72 0.66 1.0 0.98 0.99 0.98 0.97 0.98 0.98 0.97 0.96 0.98 0.97 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0.07 0.12 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.08 0.13 0.19 0.17 0.2 0.14 0.25 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 4 2 1 0 8 0 0 0 0 0 0 0 0 0 0 0 1
Bud 2 0 1 0 0 0 1 1 3 3 6 11 0 0 0 1 0 0 0 2 0 0 2 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 3 0 6 8 11 15 14 9 15 14 5 4 9 6 14 19 0 0 0 0 0 0
Cytoplasm 259 182 138 306 450 346 472 418 309 307 253 341 448 589 693 368 390 354 247 177 145 431 585 627
Endoplasmic Reticulum 1 0 0 1 6 8 12 28 13 13 4 12 0 2 3 7 8 11 1 0 0 1 2 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 1 2 3
Golgi 1 0 0 0 0 2 0 4 0 0 0 1 0 4 0 0 2 1 1 0 0 0 2 3
Mitochondria 2 1 0 1 1 7 18 18 27 29 43 24 0 0 0 1 1 0 0 0 0 0 0 2
Nucleus 0 1 2 2 3 3 10 14 8 19 7 13 1 3 3 0 1 1 0 1 2 2 3 2
Nuclear Periphery 0 0 0 0 0 0 0 2 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 11 21 31 72 113 73 87 51 129 2 6 2 7 7 4 0 0 1 1 2 3
Unique Cell Count 261 183 138 313 470 388 570 583 424 442 352 515 450 600 698 375 401 361 253 183 151 442 607 658
Labelled Cell Count 266 185 144 321 487 405 596 617 450 471 379 554 456 609 710 391 424 390 253 183 151 442 607 658


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 13.3 13.1 11.9 11.9 10.8 10.5 10.8 9.9 9.8 9.7 10.5 16.7 18.0 19.4 32.1 35.4 35.0 13.6 14.6 15.3
Std Deviation (1e-4) 1.8 2.3 1.9 2.1 2.0 1.8 1.9 2.2 2.1 2.2 2.4 2.6 3.1 3.7 3.9 8.8 10.9 10.7 2.1 2.6 2.7
Intensity Change (Log2) -0.14 -0.13 -0.28 -0.32 -0.28 -0.4 -0.42 -0.42 -0.32 0.36 0.46 0.57 1.3 1.44 1.42 0.06 0.16 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1.1 0 0 0 0 0 0 1.5
Cytoplasm -1.8 -2.5 -4.0 -5.2 -7.1 -6.9 -7.4 -7.0 -8.0 -0.8 -1.6 -1.0 -1.6 -2.0 -1.6
Endoplasmic Reticulum 0 0 0 0 2.6 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.0 3.1 4.3 2.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.4 4.4 5.6 5.2 5.7 4.7 6.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.7616 11.4986 11.057 8.879 9.5717 10.1821 8.9188 11.5424 10.6318 9.8568 7.9218 10.5161 10.5971 10.7436 11.7391 10.623 10.285 12.33
Actin 0.0002 0.0004 0.0017 0.0005 0.0006 0.0003 0.0126 0.0018 0.0076 0.0192 0.0009 0.0007 0.0103 0.0009 0.0014 0.0029 0.0585 0.0004
Bud 0.0001 0.0002 0.0038 0.0075 0.0002 0.0008 0.0012 0.0003 0.0019 0.0003 0.0002 0.0002 0.0008 0.0086 0.0018 0.0002 0.0001 0.0001
Bud Neck 0.0002 0.0002 0.0003 0.0014 0.001 0.0021 0.0025 0.0002 0.001 0.0009 0.0006 0.0013 0.001 0.0002 0.0004 0.0005 0.0005 0.0006
Bud Periphery 0 0 0.0006 0.003 0.0001 0.0002 0.0007 0.0001 0.0009 0.0004 0.0001 0.0001 0.0005 0.0012 0.0004 0.0001 0.0001 0.0001
Bud Site 0.0001 0.0006 0.0009 0.0007 0.0002 0.0003 0.0024 0.0011 0.0027 0.0003 0.0005 0.0001 0.001 0.0021 0.0022 0.0002 0.0002 0
Cell Periphery 0 0 0.0001 0.0001 0 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0003 0.0002 0.0001 0.0001 0 0
Cytoplasm 0.9326 0.9698 0.948 0.8931 0.9184 0.9202 0.8122 0.9755 0.9245 0.8752 0.7045 0.948 0.8982 0.9554 0.9605 0.9064 0.8944 0.9593
Cytoplasmic Foci 0.0037 0.002 0.0022 0.0078 0.0023 0.0077 0.0419 0.0033 0.0111 0.0144 0.0381 0.0052 0.0079 0.0028 0.0033 0.0044 0.0014 0.0014
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0.0004 0 0 0.0001 0 0 0.0001 0.0002 0
Endoplasmic Reticulum 0.0054 0.0018 0.001 0.004 0.0024 0.0022 0.0049 0.0007 0.0025 0.004 0.0063 0.0013 0.0033 0.0011 0.0009 0.0035 0.0011 0.0015
Endosome 0.0052 0.0009 0.0011 0.0179 0.0012 0.003 0.0366 0.0006 0.0106 0.0232 0.169 0.004 0.0089 0.0009 0.0051 0.0123 0.0009 0.0041
Golgi 0.0001 0.0001 0.0005 0.0008 0 0.0002 0.0076 0.0003 0.0023 0.004 0.0134 0.0019 0.0015 0.0001 0.001 0.0019 0.0018 0.0003
Lipid Particles 0.0002 0 0.0011 0.0001 0 0.0004 0.0134 0.0001 0.0029 0.0034 0.0211 0.0009 0.0015 0 0.0005 0.0022 0.0012 0.0001
Mitochondria 0.0002 0.0001 0.0053 0.0005 0.0004 0.0002 0.0034 0.0069 0.0015 0.0066 0.0046 0.0005 0.0055 0.0003 0.0004 0.0028 0.0006 0.0004
None 0.0058 0.0014 0.0016 0.0034 0.0032 0.0017 0.0102 0.0012 0.0039 0.0021 0.0025 0.0015 0.0031 0.0017 0.0012 0.0021 0.0006 0.0007
Nuclear Periphery 0.0057 0.0027 0.0025 0.0062 0.006 0.005 0.006 0.0008 0.0029 0.0041 0.0068 0.0032 0.0077 0.0037 0.0024 0.0231 0.0042 0.008
Nucleolus 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0006 0 0.0002 0.0001 0.0005 0.0002 0.0048 0.0001 0.0001 0.0002 0 0.0001
Nucleus 0.0373 0.0181 0.0259 0.0452 0.0615 0.0511 0.0155 0.0055 0.0151 0.0212 0.0155 0.0262 0.0331 0.018 0.0161 0.027 0.0281 0.0205
Peroxisomes 0.0001 0.0001 0.0019 0.0002 0 0.0002 0.0147 0.0007 0.0037 0.011 0.0016 0.0002 0.001 0.0002 0.0006 0.0002 0.0028 0
Punctate Nuclear 0.001 0.0006 0.0006 0.002 0.0014 0.003 0.0091 0.0005 0.002 0.0013 0.0026 0.0038 0.006 0.0011 0.0009 0.0022 0.0028 0.0006
Vacuole 0.0018 0.0008 0.0007 0.0047 0.0007 0.001 0.0033 0.0002 0.0016 0.006 0.0081 0.0006 0.0027 0.0011 0.0006 0.0057 0.0003 0.0011
Vacuole Periphery 0.0003 0.0001 0.0002 0.0008 0.0002 0.0002 0.0011 0.0001 0.0006 0.0019 0.0029 0.0002 0.0006 0.0002 0.0001 0.0021 0.0001 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.1044 35.7154 60.0201 65.2429 51.8007 76.3382 57.6196 61.2911 78.2062 67.4088
Translational Efficiency 2.0185 3.1839 1.7344 2.063 2.554 1.9952 2.2724 2.1042 1.876 1.9059

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication; human homolog AKR1B1 can complement yeast null mutant
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ypr1

Ypr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available