Standard name | |
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Human Ortholog | |
Description | Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 |
Bud | 1 | 0 | 0 | 1 | 1 | 2 | 5 | 8 | 11 | – | 10 | 12 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 3 | 7 | 10 | 16 |
Bud Neck | 0 | 0 | 13 | 3 | 8 | 1 | 7 | 7 | 4 | – | 9 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 5 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 3 | 8 | 6 | 10 | – | 9 | 9 | 0 | 1 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 12 | 66 | 42 | 77 | 76 | 99 | 116 | 41 | – | 61 | 72 | 31 | 58 | 86 | 90 | 47 | 40 | 7 | 10 | 26 | 5 | 19 | 18 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 2 | 0 | 3 | 0 | 1 | – | 0 | 0 | 4 | 1 | 1 | 22 | 11 | 10 | 0 | 0 | 3 | 1 | 3 | 4 |
Endosome | 0 | 0 | 0 | 5 | 1 | 4 | 2 | 1 | 4 | – | 0 | 2 | 4 | 11 | 20 | 7 | 8 | 14 | 1 | 3 | 2 | 1 | 5 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 1 |
Mitochondria | 43 | 47 | 4 | 42 | 25 | 100 | 177 | 122 | 186 | – | 265 | 158 | 43 | 18 | 24 | 35 | 2 | 20 | 1 | 2 | 7 | 1 | 18 | 21 |
Nucleus | 93 | 44 | 124 | 111 | 188 | 166 | 211 | 193 | 126 | – | 170 | 153 | 257 | 269 | 311 | 369 | 273 | 205 | 102 | 70 | 132 | 105 | 190 | 205 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Nucleolus | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 5 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 8 | 4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 3 | 8 | 12 | 8 | 12 | 23 | 2 | – | 3 | 10 | 0 | 6 | 4 | 4 | 0 | 1 | 6 | 4 | 8 | 3 | 14 | 17 |
Vac/Vac Membrane | 13 | 15 | 9 | 2 | 10 | 17 | 8 | 19 | 12 | – | 9 | 13 | 18 | 18 | 36 | 95 | 66 | 52 | 25 | 16 | 70 | 51 | 174 | 143 |
Unique Cell Count | 128 | 87 | 208 | 190 | 299 | 324 | 414 | 407 | 277 | 376 | 318 | 331 | 363 | 463 | 551 | 376 | 302 | 152 | 114 | 269 | 185 | 468 | 452 | |
Labelled Cell Count | 158 | 119 | 221 | 215 | 324 | 377 | 534 | 496 | 399 | 536 | 437 | 362 | 383 | 491 | 626 | 407 | 346 | 152 | 114 | 269 | 185 | 468 | 452 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 3.8 | 5.0 | 4.4 | 5.0 | 4.1 | 4.1 | 4.4 | 3.7 | – | 3.4 | 4.0 | 4.8 | 5.0 | 4.9 | 6.9 | 7.2 | 6.9 | 5.2 | 4.5 | 5.2 |
Std Deviation (1e-4) | 0.6 | 0.6 | 1.2 | 1.4 | 1.3 | 1.4 | 1.0 | 1.2 | 1.5 | – | 1.2 | 1.1 | 1.6 | 1.7 | 1.3 | 1.3 | 1.6 | 1.5 | 9.2 | 1.7 | 5.7 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.01 | -0.3 | -0.31 | -0.19 | -0.45 | – | -0.55 | -0.33 | -0.07 | -0.01 | -0.04 | 0.46 | 0.51 | 0.46 | 0.05 | -0.16 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.6 | 4.6 | 4.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1926 | 1.8152 | 1.6202 | 1.9017 | 1.2347 | 1.5629 | -0.7834 | -0.4788 | -0.52 | -0.4963 | -0.4382 | -0.5519 | -0.3901 | -0.1015 | -0.3089 | 0.0021 | -0.6978 | -0.3573 |
Actin | 0.1365 | 0.012 | 0.0081 | 0.0229 | 0.1049 | 0.0016 | 0.0186 | 0.0004 | 0.0086 | 0.0028 | 0.0065 | 0.0002 | 0.0298 | 0.0003 | 0.0124 | 0.0001 | 0.0023 | 0.004 |
Bud | 0.0007 | 0.0002 | 0.0001 | 0.0006 | 0.0006 | 0.0001 | 0.0006 | 0.0002 | 0.0005 | 0.0009 | 0.0003 | 0.0001 | 0.0023 | 0.0014 | 0.0008 | 0.0001 | 0.0001 | 0.0002 |
Bud Neck | 0.0162 | 0.0009 | 0.0017 | 0.0006 | 0.0054 | 0.0036 | 0.0017 | 0.0004 | 0.0011 | 0.004 | 0.0031 | 0.0022 | 0.0076 | 0.0004 | 0.0013 | 0.0025 | 0.0006 | 0.0026 |
Bud Periphery | 0.0009 | 0.0001 | 0.0001 | 0.001 | 0.0012 | 0.0001 | 0.0007 | 0.0003 | 0.0007 | 0.0011 | 0.0003 | 0.0001 | 0.0018 | 0.0006 | 0.001 | 0 | 0.0001 | 0.0002 |
Bud Site | 0.0091 | 0.0014 | 0.0035 | 0.0011 | 0.0023 | 0.0002 | 0.0042 | 0.0007 | 0.0043 | 0.003 | 0.0012 | 0.0001 | 0.004 | 0.0043 | 0.0044 | 0.0005 | 0.0002 | 0.0002 |
Cell Periphery | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.1086 | 0.0998 | 0.0553 | 0.0178 | 0.0121 | 0.0667 | 0.0471 | 0.0726 | 0.0652 | 0.0578 | 0.0417 | 0.0258 | 0.04 | 0.0603 | 0.0355 | 0.0107 | 0.0562 | 0.02 |
Cytoplasmic Foci | 0.0454 | 0.0075 | 0.0316 | 0.0039 | 0.0066 | 0.0122 | 0.0189 | 0.0061 | 0.0134 | 0.0134 | 0.0497 | 0.0016 | 0.0181 | 0.0035 | 0.0078 | 0.0073 | 0.0038 | 0.0042 |
Eisosomes | 0.0017 | 0.0001 | 0.0002 | 0.0007 | 0.0013 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0096 | 0.0093 | 0.0025 | 0.0009 | 0.0041 | 0.0029 | 0.0033 | 0.0032 | 0.0035 | 0.0022 | 0.0014 | 0.0012 | 0.0043 | 0.0021 | 0.0015 | 0.0002 | 0.0023 | 0.0022 |
Endosome | 0.028 | 0.0073 | 0.0198 | 0.0051 | 0.0129 | 0.0108 | 0.0579 | 0.0068 | 0.013 | 0.0402 | 0.0465 | 0.0025 | 0.0225 | 0.0017 | 0.0084 | 0.0152 | 0.0064 | 0.0054 |
Golgi | 0.0167 | 0.0079 | 0.0059 | 0.009 | 0.0096 | 0.0092 | 0.0094 | 0.0005 | 0.0041 | 0.0209 | 0.0093 | 0.0002 | 0.0119 | 0.0001 | 0.0056 | 0.0018 | 0.0041 | 0.002 |
Lipid Particles | 0.0378 | 0.0017 | 0.0139 | 0.0056 | 0.0319 | 0.0104 | 0.0111 | 0.0005 | 0.002 | 0.0036 | 0.0275 | 0.0002 | 0.0115 | 0.0003 | 0.0047 | 0.0037 | 0.019 | 0.0068 |
Mitochondria | 0.0037 | 0.0016 | 0.0015 | 0.1937 | 0.0074 | 0.0325 | 0.0126 | 0.0035 | 0.0061 | 0.0073 | 0.0043 | 0.0102 | 0.0095 | 0.0008 | 0.0163 | 0.0008 | 0.0016 | 0.0051 |
None | 0.2441 | 0.2138 | 0.2287 | 0.1557 | 0.0529 | 0.0996 | 0.0585 | 0.0907 | 0.0312 | 0.0388 | 0.0573 | 0.012 | 0.0572 | 0.0793 | 0.0509 | 0.1036 | 0.1229 | 0.0203 |
Nuclear Periphery | 0.016 | 0.0262 | 0.0122 | 0.0192 | 0.0069 | 0.0136 | 0.0228 | 0.0375 | 0.0274 | 0.0321 | 0.0118 | 0.0192 | 0.024 | 0.0145 | 0.0183 | 0.0054 | 0.0254 | 0.0169 |
Nucleolus | 0.0153 | 0.0139 | 0.031 | 0.0077 | 0.0135 | 0.0101 | 0.0042 | 0.003 | 0.0031 | 0.0084 | 0.0058 | 0.0093 | 0.006 | 0.0051 | 0.0061 | 0.0033 | 0.0041 | 0.0093 |
Nucleus | 0.241 | 0.5395 | 0.5107 | 0.5169 | 0.7017 | 0.6576 | 0.6857 | 0.7549 | 0.7785 | 0.7159 | 0.614 | 0.882 | 0.6929 | 0.7462 | 0.7439 | 0.7464 | 0.69 | 0.8328 |
Peroxisomes | 0.0239 | 0.0025 | 0.0067 | 0.0092 | 0.0035 | 0.0011 | 0.0034 | 0.0003 | 0.0025 | 0.0076 | 0.0371 | 0.0002 | 0.0083 | 0.0001 | 0.0062 | 0.0021 | 0.0045 | 0.0023 |
Punctate Nuclear | 0.0395 | 0.0497 | 0.0636 | 0.0249 | 0.0152 | 0.0659 | 0.0258 | 0.0158 | 0.029 | 0.0266 | 0.0778 | 0.0312 | 0.0394 | 0.0772 | 0.0721 | 0.0923 | 0.0546 | 0.064 |
Vacuole | 0.0035 | 0.0038 | 0.0025 | 0.0025 | 0.0041 | 0.0015 | 0.0083 | 0.0018 | 0.0044 | 0.0107 | 0.0032 | 0.0013 | 0.0062 | 0.0015 | 0.0017 | 0.0035 | 0.0013 | 0.0008 |
Vacuole Periphery | 0.0008 | 0.0006 | 0.0004 | 0.0012 | 0.0013 | 0.0003 | 0.0049 | 0.0008 | 0.0011 | 0.0023 | 0.0011 | 0.0004 | 0.0018 | 0.0002 | 0.0009 | 0.0004 | 0.0004 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.8716 | 8.4601 | 4.2803 | 6.0484 | 3.0676 | 3.4395 | 6.3183 | 7.0855 | 6.6438 | 5.9463 |
Translational Efficiency | 1.2019 | 0.4097 | 1.2116 | 0.8426 | 1.3701 | 1.7259 | 0.8069 | 0.74 | 0.8395 | 0.7633 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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