Standard name
Human Ortholog
Description mRNA 5'-end-capping quality-control protein; has distributive, 5'-3' exoRNase activity; similar to Rai1p;

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.96 0.97 0.98 0.96 0.86 0.79 0.77 0.74 0.72 0.66 0.68 0.98 0.98 0.99 0.96 0.95 0.94 0.85 0.9 0.84 0.82 0.81 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0.09 0.12 0.14 0.38 0.44 0.4 0.53 0.5 0.55 0.54 0 0 0 0.09 0.06 0 0.07 0 0.07 0.05 0.05 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 1 1 4
Bud 0 0 0 0 2 12 12 15 14 4 22 18 0 0 0 0 1 0 0 0 2 3 7 5
Bud Neck 0 0 1 0 0 3 6 6 1 4 3 6 2 1 0 1 1 0 0 0 0 1 1 2
Bud Site 0 0 0 0 0 0 0 2 0 1 4 2 0 0 0 0 0 0
Cell Periphery 0 0 1 0 2 1 2 6 1 3 1 1 0 0 5 1 2 4 0 0 1 0 1 2
Cytoplasm 17 102 254 228 354 391 501 518 301 321 225 314 276 303 469 172 130 100 41 102 216 167 243 232
Endoplasmic Reticulum 0 2 0 0 0 1 1 1 0 0 1 3 1 2 3 12 8 7 0 0 0 3 2 5
Endosome 0 0 0 0 0 0 0 2 0 0 0 0 2 2 1 2 2 0 0 1 3 2 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1 2 24 29 52 172 275 272 215 220 185 251 0 0 0 16 8 4 3 4 16 9 15 27
Nucleus 0 0 0 1 1 2 0 6 3 2 3 2 1 0 0 2 0 0 0 0 0 1 1 0
Nuclear Periphery 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 1 1 1 2 4 2 2 1 1 0 1 0 0 1 1 0 0 0 0 2 0
Peroxisomes 0 0 0 0 1 0 0 2 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 5 6 5 6 11 8 9 0 0 1 0 3 0 2 0 3 2 2 2
Vac/Vac Membrane 0 0 0 1 1 1 2 1 4 1 0 3 2 1 2 2 0 2 0 0 2 5 10 15
Unique Cell Count 17 106 263 232 367 453 631 672 406 443 339 464 282 308 474 180 137 106 49 113 257 205 301 310
Labelled Cell Count 18 107 281 261 416 590 807 840 548 570 453 610 284 311 481 209 156 119 49 113 257 205 301 310


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.3 3.5 3.4 3.5 3.2 2.6 3.0 2.7 2.8 2.7 2.8 5.4 5.4 5.4 5.0 5.1 5.2 6.1 6.1 5.9
Std Deviation (1e-4) 0.3 0.7 0.8 1.2 1.5 1.2 1.1 1.4 1.3 1.4 1.2 1.4 1.4 1.8 1.6 1.4 1.1 1.3 2.3 2.1 1.8
Intensity Change (Log2) -0.02 0.01 -0.1 -0.39 -0.22 -0.34 -0.32 -0.35 -0.31 0.64 0.63 0.64 0.53 0.55 0.58 0.83 0.81 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0008 0.0004 0.0012 0.0006 0.0005 0.0003 0.0145 0.0011 0.0013 0.0013 0.014 0.0036 0.0035 0.0001 0.0002 0.002 0 0.0872
Bud 0.0064 0.0082 0.0072 0.0095 0.0001 0.0072 0.0011 0.0009 0.0007 0.0002 0.0009 0.0003 0.0001 0.0002 0 0.0002 0 0.0005
Bud Neck 0.0003 0.0003 0.0003 0.0008 0.0003 0.0008 0.012 0.0001 0.0002 0.0002 0.0003 0.0005 0.0003 0 0 0.0005 0 0.0006
Bud Periphery 0.0026 0.0018 0.0022 0.0064 0.0001 0.0022 0.0027 0.0012 0.0016 0.0002 0.0017 0.0005 0.0002 0.0001 0 0.0002 0 0.0004
Bud Site 0.0063 0.0065 0.0069 0.0167 0.0001 0.0003 0.0039 0.0021 0.0041 0.0001 0.0013 0.0004 0.0009 0.0002 0.0001 0.0006 0 0.0007
Cell Periphery 0.0004 0.0004 0.0003 0.0003 0.0001 0.0002 0.0005 0.0005 0.0017 0.0003 0.0001 0.0001 0.0005 0.0001 0.0001 0.0003 0 0.0001
Cytoplasm 0.15 0.2037 0.2024 0.1646 0.2989 0.2431 0.1848 0.3389 0.2573 0.3773 0.3276 0.2325 0.2172 0.3573 0.4735 0.3119 0.0336 0.1799
Cytoplasmic Foci 0.0116 0.01 0.0133 0.0181 0.008 0.0091 0.0184 0.0145 0.01 0.0108 0.0127 0.0095 0.0142 0.0023 0.0037 0.052 0.0004 0.0309
Eisosomes 0.0003 0.0002 0.0002 0.0001 0.0014 0 0.0006 0.0001 0.0008 0.0001 0.0002 0.0001 0.0027 0 0 0.0001 0 0.0001
Endoplasmic Reticulum 0.0014 0.0009 0.0009 0.0007 0.001 0.0006 0.004 0.0028 0.0015 0.0013 0.0012 0.0014 0.0135 0.0008 0.0013 0.0315 0.0001 0.0013
Endosome 0.0021 0.0035 0.005 0.0036 0.0048 0.002 0.0125 0.0108 0.0061 0.0041 0.0131 0.0086 0.0075 0.0005 0.0009 0.1803 0 0.0626
Golgi 0.0003 0.0004 0.0004 0.0002 0.0003 0.0001 0.0051 0.0012 0.001 0.0004 0.0112 0.0027 0.0029 0.0001 0.0001 0.0098 0 0.0259
Lipid Particles 0.0008 0.0002 0.0001 0.0006 0.0001 0.0001 0.0049 0.0008 0.0026 0.0001 0.0114 0.0005 0.0452 0 0.0001 0.0233 0 0.0014
Mitochondria 0.0027 0.0009 0.0012 0.0006 0.0006 0.0005 0.0041 0.0012 0.0017 0.0003 0.0253 0.0021 0.031 0.0001 0.0001 0.0073 0 0.0095
None 0.8031 0.7534 0.7472 0.7397 0.6807 0.7272 0.7107 0.6177 0.7016 0.5993 0.5607 0.7329 0.6184 0.6373 0.5179 0.3488 0.9657 0.5873
Nuclear Periphery 0.0004 0.0007 0.0004 0.0013 0.0003 0.0004 0.0021 0.001 0.0008 0.0003 0.0011 0.0008 0.0009 0.0002 0.0003 0.0076 0 0.0004
Nucleolus 0.0017 0.0007 0.0006 0.0023 0.0001 0.0002 0.001 0.0002 0.0004 0.0001 0.0003 0.0001 0.0007 0 0 0.0011 0 0
Nucleus 0.0028 0.0041 0.0026 0.0093 0.001 0.0024 0.0016 0.001 0.0012 0.0006 0.0008 0.0007 0.0006 0.0002 0.0003 0.0036 0 0.0002
Peroxisomes 0.0003 0.0002 0.004 0.0007 0.0002 0.0002 0.0073 0.0002 0.0022 0.0001 0.0119 0.0005 0.0369 0 0 0.0026 0 0.0065
Punctate Nuclear 0.0039 0.0016 0.0027 0.0217 0.0003 0.0019 0.0018 0.0004 0.001 0.0003 0.0014 0.0004 0.0006 0.0001 0.0001 0.0055 0 0.0002
Vacuole 0.0015 0.0017 0.001 0.002 0.001 0.0008 0.0053 0.0028 0.002 0.0022 0.0019 0.0013 0.0019 0.0004 0.0011 0.008 0 0.0032
Vacuole Periphery 0.0002 0.0003 0.0002 0.0003 0.0002 0.0001 0.0012 0.0005 0.0003 0.0003 0.001 0.0003 0.0003 0 0.0001 0.0029 0 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.2655 13.3595 14.87 17.5288 17.7615 6.6383 16.3219 16.461 14.1446 17.9651
Translational Efficiency 0.6581 0.6105 0.6095 0.461 0.4963 1.3307 0.4962 0.5159 0.543 0.636

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description mRNA 5'-end-capping quality-control protein; has distributive, 5'-3' exoRNase activity; similar to Rai1p;
Localization
Cell Percentages cytoplasm (36%), nucleus (7%), mixed (47%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dxo1

Dxo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dxo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available