Standard name
Human Ortholog
Description Putative chitinase; functionally complements A. gossypii cts2 mutant sporulation defect
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 1.0 0.98 0.92 0.84 0.82 – 0.82 0.76 0.77 0.99 0.99 0.99 0.97 0.99 0.99 0.97 0.93 0.97 0.9 0.91 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.2 0.38 0.31 – 0.38 0.46 0.46 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 1 2
Bud 0 0 3 0 1 5 3 15 – 11 9 10 3 0 0 1 0 0 0 0 0 0 3 8
Bud Neck 0 0 0 0 0 2 0 2 – 3 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 3 0 2 – 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 3 3 1 5 2 – 5 1 7 0 0 2 4 2 7 0 0 0 0 0 0
Cytoplasm 241 141 224 341 561 553 637 689 – 485 369 514 216 299 365 221 236 265 236 136 224 116 265 319
Endoplasmic Reticulum 0 3 1 0 1 2 1 0 – 0 0 3 1 1 0 16 10 13 0 1 0 0 3 2
Endosome 0 0 0 0 0 0 1 1 – 0 0 0 0 0 0 2 0 0 0 1 1 1 1 4
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 1 0 0 1 0 0 1 1
Mitochondria 1 0 3 13 29 119 286 261 – 228 223 311 0 0 1 3 2 1 2 0 2 2 4 9
Nucleus 0 0 1 1 1 2 5 13 – 4 1 6 0 1 2 1 1 0 0 1 0 0 0 1
Nuclear Periphery 0 0 0 0 2 0 14 5 – 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 1 2 – 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 1 0 0 0 0 0 0 1 1
Vac/Vac Membrane 2 0 0 0 1 2 4 11 – 11 12 5 1 0 1 3 0 1 0 0 0 2 2 5
Unique Cell Count 244 144 227 342 570 602 759 839 594 485 669 219 301 368 227 238 267 243 147 231 130 291 366
Labelled Cell Count 244 144 233 359 599 689 957 1003 749 618 858 221 303 372 253 252 287 243 147 231 130 291 366


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.8 4.0 3.9 4.1 4.0 3.4 3.7 – 3.4 3.3 3.4 6.0 5.9 6.3 6.0 5.8 6.2 6.4 6.7 6.8
Std Deviation (1e-4) 0.7 1.1 0.5 0.9 1.0 1.1 0.9 1.0 – 0.8 0.9 0.7 1.3 1.2 1.6 1.1 0.9 1.3 1.9 2.1 1.9
Intensity Change (Log2) – – – -0.03 0.03 -0.02 -0.25 -0.12 – -0.23 -0.26 -0.22 0.58 0.55 0.65 0.57 0.54 0.64 0.67 0.75 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 -0.3 -3.6 -5.9 -6.3 – -6.4 -7.5 -7.5 0 0.8 0.6 -1.0 0.5 0.6
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 3.7 2.7 3.0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0211 0.0003 0.0024 0.0015 0.0025 0.0043 0.1016 0.0011 0.0305 0.0156 0.0002 0.0124 0.029 0.0018 0.0295 0.0095 0.0195 0.0083
Bud 0.0022 0.0093 0.0009 0.0007 0.0065 0.0008 0.0017 0.0002 0.001 0.0014 0 0.0002 0.0012 0.0002 0.0006 0.0009 0.0003 0.0005
Bud Neck 0.0041 0.0002 0.0033 0.0004 0.0006 0.0004 0.01 0.0001 0.0005 0.0079 0 0.0002 0.0009 0.0001 0.0138 0.0142 0.0008 0.0009
Bud Periphery 0.0024 0.0013 0.0018 0.0011 0.0024 0.0021 0.0026 0.0003 0.0024 0.007 0 0.0006 0.0012 0.0003 0.0006 0.0004 0.0003 0.0006
Bud Site 0.0154 0.0041 0.0028 0.0003 0.0004 0.0001 0.0244 0.0004 0.0043 0.0007 0 0.0001 0.0054 0.0011 0.0069 0.004 0.0012 0.0022
Cell Periphery 0.0006 0.0004 0.0002 0 0.0001 0.0001 0.0005 0.0001 0.0003 0.0008 0.0001 0.0001 0.0005 0.0002 0.0004 0.0001 0.0001 0.0002
Cytoplasm 0.252 0.3404 0.3077 0.241 0.2755 0.309 0.1018 0.2841 0.2633 0.1657 0.2238 0.3181 0.3382 0.4143 0.5289 0.0261 0.6311 0.64
Cytoplasmic Foci 0.0127 0.0056 0.007 0.0063 0.0097 0.0067 0.024 0.0059 0.0146 0.0069 0.0028 0.005 0.0245 0.0107 0.0285 0.5815 0.0642 0.0181
Eisosomes 0.0006 0.0001 0.0002 0.0001 0 0.0001 0.001 0.0002 0.0003 0.0003 0 0.0001 0.0003 0.0001 0.0002 0 0.0001 0.0001
Endoplasmic Reticulum 0.0027 0.0014 0.0019 0.0011 0.0008 0.0008 0.0064 0.0012 0.0034 0.003 0.0005 0.0019 0.022 0.0031 0.0059 0.0028 0.002 0.0041
Endosome 0.0068 0.0012 0.0015 0.0011 0.0007 0.0014 0.0145 0.0006 0.0054 0.0396 0.0006 0.0103 0.1071 0.0035 0.0433 0.1905 0.0648 0.0558
Golgi 0.0019 0.0001 0.0002 0.0001 0.0001 0.0006 0.0062 0.0002 0.003 0.0035 0 0.0036 0.0227 0.0013 0.0208 0.0862 0.078 0.0382
Lipid Particles 0.0031 0.0002 0.0003 0.0001 0.0001 0.0007 0.0095 0.0002 0.0013 0.001 0 0.0011 0.0078 0.0009 0.0104 0.0285 0.0773 0.0095
Mitochondria 0.0018 0.0004 0.0004 0.0005 0.0004 0.0002 0.0046 0.0002 0.0013 0.0019 0.0001 0.0017 0.0081 0.0005 0.0078 0.0033 0.0061 0.0342
None 0.6582 0.6252 0.6626 0.7291 0.6815 0.6668 0.6531 0.7033 0.6564 0.7185 0.7708 0.6403 0.3759 0.5598 0.274 0.0009 0.0353 0.1743
Nuclear Periphery 0.002 0.001 0.0008 0.0012 0.0007 0.0009 0.0051 0.0003 0.0011 0.0049 0.0002 0.0017 0.0166 0.0005 0.0013 0.0011 0.001 0.0011
Nucleolus 0.0011 0.0007 0.0003 0.0007 0.0008 0.0002 0.0026 0.0001 0.0002 0.0005 0 0.0001 0.001 0.0001 0.0004 0.0003 0.0001 0.0003
Nucleus 0.0019 0.0043 0.0028 0.0038 0.0097 0.0015 0.003 0.0006 0.0014 0.0017 0.0003 0.0009 0.0077 0.0004 0.001 0.0008 0.0007 0.0014
Peroxisomes 0.0034 0.0001 0.0004 0.0003 0.0004 0.0002 0.0082 0.0003 0.0054 0.0006 0.0001 0.0003 0.0023 0.0003 0.0209 0.0343 0.0135 0.005
Punctate Nuclear 0.0046 0.0025 0.0021 0.01 0.0065 0.0028 0.0168 0.0004 0.0028 0.0026 0.0001 0.0004 0.0195 0.0003 0.0006 0.0113 0.002 0.0005
Vacuole 0.0009 0.0012 0.0006 0.0004 0.0004 0.0003 0.0018 0.0003 0.0008 0.0125 0.0003 0.0005 0.0051 0.0006 0.0037 0.0022 0.001 0.0034
Vacuole Periphery 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0.0007 0 0.0002 0.0033 0 0.0004 0.003 0.0001 0.0007 0.0008 0.0006 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5223 11.5591 7.1478 15.6506 12.5737 8.6156 11.0381 12.2705 14.2971 14.1309
Translational Efficiency 0.8443 0.5888 1.0334 0.5391 0.6597 1.5658 0.8136 0.7265 0.6197 0.7623

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative chitinase; functionally complements A. gossypii cts2 mutant sporulation defect
Localization
Cell Percentages cytoplasm (81%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cts2

Cts2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cts2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available