Standard name
Human Ortholog
Description N-myristoylated calcium-binding protein; may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily; human NCS1 functionally complements the heat sensitivity of a frq1 ts mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.19 0.26 0.08 0.07 0.05 0.13 0.05 0.05 0.06 0 0.05 0.08 0.31 0.18 0.11 0.18 0.21 0.37 0.1 0.12 0.11 0.08 0.13 0.14
Bud 0 0 0.1 0 0.07 0.07 0.06 0.08 0.08 0.05 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0.08 0.11 0.09 0.05 0.07 0.06 0.06 0.09 0.07 0.08 0.12 0.15 0.18 0.28 0.25 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.17 0 0.07 0 0.06 0.05 0 0 0 0 0.05 0.19 0.19 0.12 0.07 0.06 0 0 0 0 0 0 0
Endosome 0.25 0.11 0.15 0.34 0.21 0.1 0.07 0.14 0.1 0.11 0.05 0.06 0.2 0.25 0.29 0.3 0.25 0.26 0.31 0.23 0.3 0.25 0.19 0.25
Golgi 0.42 0.52 0.14 0.19 0.15 0.08 0 0 0 0 0 0 0.24 0.23 0.24 0.19 0.22 0.19 0.44 0.45 0.4 0.36 0.38 0.4
Mitochondria 0.33 0.23 0.66 0.4 0.57 0.75 0.78 0.67 0.77 0.72 0.78 0.72 0.05 0.06 0 0.1 0.12 0 0 0.05 0 0.09 0.07 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0.05 0 0 0.07 0 0 0.06 0 0 0 0 0 0 0 0.05 0 0.05 0.08 0.05 0
SpindlePole 0.07 0 0 0 0 0.05 0 0.05 0 0.06 0 0.05 0.05 0.06 0.05 0.1 0.06 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.12 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 36 64 10 12 13 43 26 20 21 14 14 33 55 27 24 17 26 35 22 37 19 11 32 38
Bud 6 3 13 6 19 24 28 31 30 20 30 37 7 1 1 0 5 4 0 5 0 3 6 3
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 2
Bud Site 0 0 0 0 0 5 9 9 8 6 10 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 2 0 1 8 1 1 2 3 9 1 1 2 0 2 0 0 0 0 0 0 0
Cytoplasm 6 21 10 18 27 15 34 23 23 40 20 35 22 23 40 27 31 18 1 5 2 1 3 1
Endoplasmic Reticulum 11 41 1 11 12 21 27 9 11 14 11 22 33 30 27 7 7 2 1 4 1 2 8 10
Endosome 48 26 19 57 61 33 35 58 36 49 14 24 36 38 64 29 31 25 70 71 51 37 48 71
Golgi 79 127 18 32 43 27 12 8 8 12 5 6 43 36 52 18 27 18 102 141 68 53 94 112
Mitochondria 63 56 83 66 162 248 390 272 285 310 221 297 8 9 4 10 15 4 10 15 6 12 17 19
Nucleus 0 0 1 0 2 2 4 1 1 3 1 3 0 1 0 0 0 1 0 2 0 0 0 0
Nuclear Periphery 0 0 1 1 0 0 2 1 0 7 0 2 0 1 0 0 0 0 0 0 1 0 0 1
Nucleolus 1 2 3 0 6 7 12 20 12 14 15 13 1 0 2 0 0 1 0 0 0 0 1 0
Peroxisomes 3 2 9 5 14 8 14 28 12 19 16 11 5 1 0 0 0 0 12 6 8 11 11 7
SpindlePole 13 4 3 7 6 15 14 19 13 26 12 21 9 9 12 10 8 8 4 6 4 4 6 1
Vac/Vac Membrane 14 11 3 4 6 5 9 7 10 9 10 6 9 7 26 4 7 3 3 10 3 5 8 7
Unique Cell Count 190 242 126 166 285 331 501 406 371 430 284 415 176 154 219 97 124 95 230 313 169 149 247 283
Labelled Cell Count 281 357 174 221 371 454 624 508 471 545 382 530 229 184 254 122 159 119 230 313 169 149 247 283


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 9.9 7.7 7.7 7.5 7.4 6.8 7.0 6.8 6.3 6.4 6.4 9.5 8.9 9.3 11.0 11.2 11.2 10.0 10.9 10.8
Std Deviation (1e-4) 1.4 1.4 1.8 1.7 1.5 1.3 1.6 1.8 1.6 1.5 1.5 1.5 1.4 1.5 1.2 2.5 2.5 2.3 1.6 1.8 1.9
Intensity Change (Log2) – – – 0.01 -0.05 -0.06 -0.17 -0.14 -0.17 -0.3 -0.26 -0.26 0.31 0.21 0.27 0.51 0.53 0.54 0.38 0.5 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -1.4 1.5 -1.2 -1.3 -0.9 -2.3 -1.2 0 4.9 2.4 0.9 2.2 2.9 5.3
Bud -2.3 -1.3 -1.1 -1.9 -1.0 -0.8 -2.4 0.1 -0.5 -2.2 -3.7 -4.5 -3.3 -1.9 -1.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.5 -1.4 -0.5 -0.9 -0.7 0.5 -0.3 0.2 1.3 1.8 2.6 4.0 3.6 2.4
Endoplasmic Reticulum 2.5 0 2.5 2.2 0 0 0 0 2.2 4.9 5.0 3.8 0 0 0
Endosome 3.7 1.5 -1.5 -2.9 -0.2 -1.7 -1.1 -3.5 -3.4 1.2 2.0 3.0 2.7 2.0 2.1
Golgi 1.1 0.2 -2.0 -5.6 -5.6 -5.3 -5.0 -5.1 -6.1 2.2 1.9 2.1 0.9 1.5 0.9
Mitochondria -4.4 -1.7 1.9 2.8 0.2 2.4 1.3 2.5 1.2 -11.5 -10.6 -13.2 -8.3 -8.7 -9.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1.2 0 0 1.3 0 0 0 0 0 0 0
Peroxisomes -1.6 -0.9 0 0 -0.1 -1.9 -1.2 -0.6 0 -1.8 0 0 0 0 0
SpindlePole 0 0 0 0 1.1 0 1.6 0 1.3 1.2 1.4 1.4 2.5 1.6 0
Vacuole 0 0 0 0 0 0 0 0 0 1.2 0 3.1 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0445 0.0391 0.0428 0.004 0.0309 0.0271 0.0617 0.0373 0.0506 0.0153 0.0331 0.0778 0.2587 0.1279 0.0853 0.0295 0.001 0.133
Bud 0.0004 0.0013 0.0005 0.0001 0.0011 0.0003 0.0004 0.0005 0.0006 0.0002 0.0005 0.0003 0.004 0.0014 0.0012 0.0006 0.0004 0.0007
Bud Neck 0.0017 0.0011 0.0012 0.0005 0.002 0.0026 0.0017 0.0013 0.0026 0.0023 0.0023 0.002 0.0032 0.003 0.0008 0.003 0.003 0.0177
Bud Periphery 0.0003 0.0009 0.0004 0.0001 0.001 0.0003 0.0004 0.0002 0.0004 0.0001 0.0005 0.0003 0.0035 0.0006 0.0026 0.0004 0.0001 0.0006
Bud Site 0.0027 0.0207 0.003 0.0003 0.0056 0.0007 0.0039 0.0111 0.0095 0.0003 0.001 0.0007 0.1118 0.0687 0.0018 0.0008 0.0033 0.0011
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0016 0.0003 0.0004 0.0001 0.0002 0.0002
Cytoplasm 0.025 0.035 0.0085 0.0096 0.0092 0.0098 0.0133 0.0146 0.0057 0.0052 0.0368 0.0122 0.0358 0.0436 0.0036 0.0477 0.1682 0.0647
Cytoplasmic Foci 0.2174 0.26 0.2328 0.2175 0.2446 0.1897 0.1845 0.2589 0.1999 0.2121 0.1859 0.1971 0.1355 0.1951 0.0768 0.1217 0.3177 0.1587
Eisosomes 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001 0.0002 0 0.0002 0 0 0.0001 0.0012 0.0002 0.0017 0.0001 0 0.0001
Endoplasmic Reticulum 0.0055 0.0009 0.001 0.0016 0.0007 0.0015 0.0095 0.0015 0.0008 0.0007 0.0009 0.0011 0.0017 0.0033 0.004 0.0013 0.0078 0.004
Endosome 0.3083 0.2601 0.2209 0.2839 0.2014 0.2156 0.2422 0.2238 0.1937 0.2609 0.2774 0.1875 0.0757 0.174 0.2137 0.3344 0.4096 0.1706
Golgi 0.3043 0.3378 0.4109 0.4111 0.4644 0.4917 0.3883 0.3961 0.445 0.4506 0.3824 0.4638 0.1492 0.3241 0.5523 0.4423 0.0624 0.3959
Lipid Particles 0.0239 0.0083 0.0154 0.0161 0.0031 0.0093 0.0259 0.0098 0.015 0.0056 0.0065 0.0134 0.0095 0.0189 0.0059 0.0017 0.0082 0.0274
Mitochondria 0.0082 0.0028 0.0059 0.0025 0.012 0.0113 0.018 0.0034 0.0128 0.0061 0.0057 0.007 0.0117 0.0156 0.0405 0.0091 0.0021 0.0087
None 0.0055 0.0003 0.0002 0.0404 0.0001 0.0076 0.0068 0.0001 0.0105 0.0002 0.0003 0.0003 0.1392 0.0007 0.001 0.0001 0.0007 0.0005
Nuclear Periphery 0.0059 0.0002 0.0003 0.001 0.0001 0.0006 0.0041 0.0001 0.0005 0.0002 0.0005 0.0003 0.0065 0.0004 0.0008 0.0001 0.001 0.0004
Nucleolus 0.0034 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0021 0.0001 0.0001 0 0.0001 0
Nucleus 0.0034 0.0001 0.0001 0.0003 0.0003 0.0002 0.0005 0.0002 0.0002 0.0001 0.0004 0.0002 0.0038 0.0001 0.0002 0.0001 0.0009 0.0002
Peroxisomes 0.0281 0.0286 0.0532 0.0071 0.0197 0.0286 0.0311 0.0383 0.049 0.0385 0.0554 0.0331 0.0225 0.0197 0.0041 0.0027 0.0028 0.0128
Punctate Nuclear 0.0056 0.0006 0.001 0.0016 0.0003 0.0007 0.0028 0.0012 0.0009 0.0004 0.0003 0.0011 0.0202 0.0007 0.0002 0.0001 0.0017 0.0006
Vacuole 0.0039 0.0013 0.0009 0.001 0.002 0.0011 0.0027 0.0008 0.001 0.0006 0.0064 0.001 0.0017 0.001 0.0015 0.0022 0.0073 0.0014
Vacuole Periphery 0.0016 0.0007 0.0007 0.0008 0.0009 0.0009 0.0016 0.0005 0.0007 0.0005 0.0037 0.0007 0.0008 0.0005 0.0016 0.0018 0.0014 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 64.5097 55.0856 52.3722 61.5028 54.9275 67.3609 67.4456 67.5476 61.6271 64.2933
Translational Efficiency 1.6196 1.6616 1.8314 1.7586 1.5931 1.6457 1.588 1.5582 1.8765 1.5589

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description N-myristoylated calcium-binding protein; may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily; human NCS1 functionally complements the heat sensitivity of a frq1 ts mutant
Localization
Cell Percentages Golgi (6%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Frq1

Frq1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Frq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available