Standard name
Human Ortholog
Description Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane and delivers it to an assembly intermediate of respiratory Complex III; also required for assembly of the Qcr10p subunit; mutation is functionally complemented by human homolog BCS1L, linked to neonatal diseases
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.12 0.1 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0.08 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0.07 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.14 – – – 0 0 0 0 0 0
Endosome 0.18 0 0.09 0 0.21 0.11 0 0 0 0 0 0 0 0.06 0.19 – – – 0.19 0.13 0.16 0.14 0.12 0.14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.08 – – – 0.05 0.05 0.05 0.06 0.07 0.08
Mitochondria 0.89 0.95 0.87 0.95 0.76 0.75 0.85 0.84 0.91 0.95 0.95 0.93 0.83 0.53 0.28 – – – 0.56 0.61 0.51 0.49 0.48 0.46
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0.07 0.07 0 0 0.06 0.08 0.05 0.06 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0.09 0 0.14 0.1 0.16 0.05 0.07 0.09 0.07 0 0 0.05 0 0 0 – – – 0.13 0.05 0.21 0.17 0.2 0.18
SpindlePole 0.07 0 0.05 0 0.07 0.05 0.07 0.08 0.09 0.05 0.06 0.06 0.17 0.1 0.1 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.11 0 0 0 0 0 0 0 0.2 0.14 – – – 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 7 0 0 0 0 0 0 0 0 0 0 4 6 10 – – – 1 4 6 1 5 6
Bud 4 2 6 8 4 4 7 8 14 18 11 11 1 4 3 – – – 1 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 – – – 0 0 1 0 1 2
Bud Site 1 0 1 1 1 5 9 2 8 7 6 7 0 0 0 – – – – – – – – –
Cell Periphery 0 1 2 3 2 1 1 1 1 1 0 2 1 2 3 – – – 0 0 0 0 0 0
Cytoplasm 0 4 8 3 3 7 10 15 11 9 8 8 1 1 7 – – – 0 1 0 0 0 0
Endoplasmic Reticulum 0 8 0 0 0 0 1 0 0 2 0 0 2 3 14 – – – 0 0 1 0 3 0
Endosome 27 1 31 5 58 24 13 7 0 2 0 4 0 3 19 – – – 31 22 50 9 26 27
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 6 8 – – – 7 8 17 3 14 16
Mitochondria 134 169 285 196 204 167 344 211 204 282 178 276 30 26 28 – – – 91 111 163 33 106 91
Nucleus 0 3 0 0 1 5 9 6 4 3 1 0 0 0 0 – – – 0 0 0 0 1 0
Nuclear Periphery 2 3 12 2 5 3 11 5 10 14 6 8 1 0 1 – – – 1 0 2 1 3 2
Nucleolus 7 3 11 6 10 16 30 11 8 18 15 14 2 0 0 – – – 0 2 0 1 1 4
Peroxisomes 14 0 45 20 42 11 30 22 15 5 2 15 0 0 2 – – – 21 9 68 11 44 34
SpindlePole 10 2 17 6 18 11 30 21 21 14 12 19 6 5 10 – – – 2 4 4 2 2 1
Vac/Vac Membrane 6 8 14 3 14 24 10 3 2 2 0 7 0 10 14 – – – 1 11 2 1 4 5
Unique Cell Count 151 178 327 207 270 224 404 250 225 298 188 298 36 49 100 163 182 322 69 222 199
Labelled Cell Count 205 212 432 253 362 278 505 312 298 378 239 371 49 66 120 163 182 322 69 222 199


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.0 5.1 5.2 5.2 5.0 3.9 3.7 3.6 3.2 3.4 3.3 5.4 5.8 6.3 – – – 7.3 7.2 7.1
Std Deviation (1e-4) 0.6 0.8 1.3 1.2 1.3 2.3 1.0 1.4 1.2 1.0 1.6 0.7 0.7 0.6 1.2 – – – 1.7 2.2 1.7
Intensity Change (Log2) – – – 0.03 0.02 -0.02 -0.4 -0.46 -0.51 -0.66 -0.57 -0.63 0.07 0.17 0.31 – – – 0.51 0.49 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 1.4 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0 0 0.5 0 2.2 1.5 0.4 1.1 0.2 0 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome -3.2 4.1 0.5 0 0 0 0 0 0 0 0 2.6 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 2.8 -3.7 -3.8 0 0 0 0 0 0 -0.6 -5.9 -11.7 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery -1.9 -1.3 -1.7 0 0 0 0 0 0 0 0 0 – – –
Nucleolus -0.3 0.2 2.0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes -1.4 0.6 -3.4 0 0 0 0 0 0 0 -2.8 -3.3 – – –
SpindlePole -1.3 0.8 -0.2 0 0 0 0 0 0 0 0 1.7 – – –
Vacuole -1.8 0.5 2.9 -1.4 -2.2 -2.3 -2.9 -2.9 -1.3 0 0 3.4 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4618 2.7953 2.6032 2.3972 2.261 2.7083 3.7005 4.5907 4.0578 3.7699 4.0137 4.2288 3.6324 4.1018 3.7471 3.2612 2.6564 3.7487
Actin 0.014 0.0132 0.0104 0.0115 0.0337 0.0057 0.0325 0.0249 0.0185 0.0024 0.0241 0.0262 0.0245 0.0143 0.0228 0.0061 0.0013 0.031
Bud 0.0003 0.0034 0.0013 0.0005 0.0005 0.0002 0.0004 0.0013 0.0007 0.0001 0.0004 0.0003 0.0003 0.0003 0.0003 0.0002 0.0001 0.0002
Bud Neck 0.0009 0.0006 0.0002 0.0011 0.0003 0.0003 0.0007 0.0007 0.0008 0.0002 0.0002 0.0005 0.0004 0.0003 0.0003 0.0005 0.0001 0.0007
Bud Periphery 0.0005 0.0026 0.0019 0.0007 0.0031 0.0003 0.0007 0.0018 0.0014 0.0003 0.0006 0.0006 0.0004 0.0005 0.0005 0.0003 0.0002 0.0005
Bud Site 0.0016 0.0043 0.0018 0.0009 0.0004 0.0003 0.0018 0.0082 0.0019 0.0003 0.0006 0.0005 0.0011 0.0013 0.0012 0.0006 0.0001 0.0004
Cell Periphery 0.0003 0.0005 0.0002 0.0002 0.0003 0.0001 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002
Cytoplasm 0.0007 0.008 0.0008 0.0016 0.0004 0.0004 0.0096 0.0021 0.0027 0.0008 0.0004 0.0015 0.0007 0.0003 0.0003 0.0003 0.0001 0.0003
Cytoplasmic Foci 0.0448 0.0495 0.0221 0.0317 0.0129 0.0153 0.0413 0.0479 0.0464 0.0544 0.0394 0.0247 0.0397 0.0322 0.0262 0.0488 0.0133 0.0161
Eisosomes 0.0003 0.0002 0.0002 0.0002 0.0008 0.0001 0.0003 0.0003 0.0002 0.0001 0.0003 0.0003 0.0003 0.0002 0.0003 0.0002 0.0001 0.0003
Endoplasmic Reticulum 0.0007 0.001 0.0003 0.0016 0.0016 0.0002 0.0063 0.0004 0.0006 0.0002 0.0001 0.001 0.0002 0.0001 0.0003 0.0001 0.0001 0.0002
Endosome 0.0844 0.112 0.0445 0.0707 0.0589 0.0469 0.0953 0.1218 0.0672 0.0558 0.0505 0.0434 0.0632 0.0513 0.0613 0.0449 0.0433 0.0408
Golgi 0.113 0.1231 0.0673 0.0794 0.0574 0.0615 0.0962 0.1758 0.0825 0.085 0.097 0.1089 0.0887 0.169 0.0808 0.0775 0.0699 0.0941
Lipid Particles 0.1328 0.0824 0.0619 0.0301 0.0471 0.0865 0.0977 0.0537 0.1039 0.0235 0.0711 0.0569 0.1622 0.0464 0.065 0.0433 0.0312 0.0436
Mitochondria 0.4386 0.4593 0.6641 0.6049 0.7082 0.6778 0.4661 0.4359 0.5231 0.6964 0.5144 0.6028 0.4278 0.5504 0.5939 0.5799 0.6901 0.621
None 0.0006 0.007 0.0002 0.0007 0.002 0.0001 0.0025 0.0006 0.0026 0.0004 0.0007 0.0013 0.0031 0.0001 0.0002 0.0002 0.0001 0.0004
Nuclear Periphery 0.0009 0.0053 0.001 0.0193 0.0007 0.0002 0.0056 0.0002 0.0038 0.0001 0 0.0002 0.0013 0.0001 0.0003 0.0001 0.0001 0.0002
Nucleolus 0.0006 0.0018 0.0003 0.0009 0.0002 0.0003 0.001 0.0005 0.001 0.0001 0.0001 0.0002 0.0018 0.0003 0.0008 0.0002 0.0001 0.0005
Nucleus 0.0002 0.0012 0.0002 0.02 0.0002 0.0001 0.0007 0.0002 0.0006 0.0001 0.0001 0.0001 0.0004 0.0001 0.0003 0.0001 0.0001 0.0002
Peroxisomes 0.1563 0.1027 0.1145 0.1162 0.0657 0.0969 0.1196 0.1141 0.1294 0.0737 0.1982 0.1263 0.1726 0.1296 0.1304 0.1931 0.1459 0.144
Punctate Nuclear 0.0014 0.001 0.0002 0.0021 0.0002 0.0001 0.0042 0.0003 0.0014 0.0026 0.0002 0.0002 0.0047 0.0001 0.0013 0.0003 0.0001 0.0002
Vacuole 0.0042 0.0138 0.0034 0.0026 0.0019 0.0026 0.0088 0.0045 0.0058 0.0013 0.0006 0.0017 0.0034 0.0013 0.0074 0.0013 0.0013 0.0016
Vacuole Periphery 0.0028 0.0071 0.0033 0.003 0.0035 0.0039 0.0086 0.0045 0.0053 0.002 0.001 0.0023 0.0031 0.0016 0.0059 0.002 0.0024 0.0036

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.9012 15.1086 9.6096 16.3772 14.6087 19.3049 18.9135 23.3213 18.7088 21.6936
Translational Efficiency 0.6741 0.5697 1.0706 0.6386 0.7158 0.7012 0.711 0.7183 0.8148 0.6209

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bcs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available