Standard name
Human Ortholog
Description Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0.07 0.12 0.07 – 0.08 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.99 0.96 0.91 0.84 0.86 – 0.9 0.82 0.78 0.74 0.99 0.97 0.99 0.98 0.98 0.98 0.93 0.97 0.88 0.78 0.78 0.83
Endoplasmic Reticulum 0 0 0 0 0.06 0.06 0 – 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0.09 0.1 0.08
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.05 0.12 0.18 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 – 0.05 0.08 0.05 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 3 – 3 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 1 5 – 2 5 4 2 0 0 0 0 0 0 0 1 3 3 9 8
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 6 13 6 12 24 16 – 17 11 8 8 0 5 0 3 5 0 0 0 2 0 1 0
Cytoplasm 69 276 205 182 157 165 208 – 203 176 114 105 171 226 240 217 157 140 79 278 193 124 252 282
Endoplasmic Reticulum 0 1 2 6 10 12 7 – 2 2 2 0 1 3 1 10 6 9 1 2 5 14 30 27
Endosome 0 0 0 0 0 0 1 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 0 0 0 1 1 3 1 – 1 0 0 1 0 0 1 2 0 1 0 0 0 0 4 2
Mitochondria 0 0 0 0 0 0 3 – 11 25 27 20 0 1 0 0 0 0 0 0 0 2 3 3
Nucleus 0 0 0 0 2 0 2 – 2 2 3 3 0 0 0 0 0 0 1 0 2 0 0 1
Nuclear Periphery 0 1 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 2 – 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
SpindlePole 0 0 0 0 0 1 2 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 1 0 4 16 – 11 18 7 16 0 1 0 1 0 2 1 1 4 3 7 6
Unique Cell Count 69 277 208 190 173 196 243 226 215 147 142 173 232 242 222 161 143 86 287 220 159 322 342
Labelled Cell Count 69 284 222 196 182 212 266 254 240 166 158 173 236 242 233 168 152 86 287 220 159 322 342


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 176.9 139.0 100.3 112.9 74.3 35.6 15.0 – 8.5 8.2 8.3 7.9 144.0 132.4 111.2 200.9 215.6 177.0 115.5 110.7 107.3
Std Deviation (1e-4) 46.2 36.2 32.7 36.5 25.6 18.3 7.1 – 3.9 3.2 3.2 4.7 48.8 37.7 35.7 68.2 68.7 71.4 48.5 42.1 44.0
Intensity Change (Log2) – – – 0.17 -0.43 -1.5 -2.74 – -3.56 -3.61 -3.6 -3.67 0.52 0.4 0.15 1.0 1.1 0.82 0.2 0.14 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 0.3 2.1 0.1 – 0.5 -0.5 -0.3 -0.2 -3.3 -2.2 -3.9 -2.7 -1.4 -3.0
Cytoplasm -1.7 -3.5 -5.2 -4.9 – -3.8 -5.7 -6.5 -7.1 0.2 -0.8 0.6 -0.6 -0.7 -0.5
Endoplasmic Reticulum 0 2.7 2.8 0 – 0 0 0 0 0 0 0 2.2 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.2 5.1 6.4 5.6 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.0 – 2.4 3.6 0 4.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 149.163 229.5001 201.1202 191.2439 164.1852 162.0249 174.9533 286.1682 260.2753 259.748 221.6409 240.4365 173.8485 258.4984 228.3718 205.6086 176.7723 244.644
Actin 0.0131 0.0006 0.0047 0.0549 0.004 0.0137 0.0139 0.0006 0.0014 0.0005 0.0069 0.0017 0.0136 0.0005 0.0118 0.0004 0.0004 0.0015
Bud 0.0035 0.0015 0.0023 0.0028 0.0043 0.0201 0.0079 0.0021 0.0034 0.0086 0.007 0.0088 0.0026 0.0025 0.0023 0.0027 0.0043 0.0035
Bud Neck 0.0014 0.0012 0.0019 0.0025 0.0019 0.0063 0.0011 0.0009 0.001 0.0013 0.0022 0.003 0.0011 0.0007 0.0014 0.0005 0.0008 0.0024
Bud Periphery 0.0037 0.0015 0.0017 0.0022 0.0072 0.0102 0.0102 0.0012 0.0044 0.0094 0.0102 0.0134 0.0027 0.0017 0.0031 0.0027 0.0031 0.0029
Bud Site 0.0053 0.0014 0.0066 0.0033 0.0012 0.0022 0.0036 0.0016 0.0015 0.0026 0.001 0.0005 0.0013 0.0013 0.0012 0.0008 0.0013 0.0004
Cell Periphery 0.0587 0.0558 0.0662 0.0554 0.0337 0.0127 0.0665 0.0365 0.0536 0.0371 0.0214 0.0336 0.0751 0.047 0.0578 0.0796 0.0354 0.0218
Cytoplasm 0.3798 0.7639 0.5588 0.4864 0.3806 0.4497 0.4477 0.8342 0.7273 0.4611 0.6178 0.5573 0.4902 0.8119 0.6558 0.6183 0.4742 0.7597
Cytoplasmic Foci 0.0517 0.0006 0.0223 0.003 0.006 0.0122 0.0169 0.0007 0.0058 0.0018 0.0025 0.0056 0.0201 0.001 0.0041 0.0014 0.0052 0.0034
Eisosomes 0.0027 0 0.0014 0.001 0.0001 0.0006 0.0039 0.0001 0.0004 0.0001 0.0006 0.0003 0.0016 0.0001 0.0004 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0142 0.0051 0.0138 0.0016 0.0118 0.0251 0.0229 0.0024 0.0064 0.0043 0.0025 0.007 0.024 0.009 0.0138 0.0078 0.0029 0.0272
Endosome 0.0096 0.002 0.0046 0.0046 0.0217 0.0354 0.0122 0.001 0.0029 0.0044 0.0071 0.0152 0.0088 0.0011 0.0057 0.0018 0.0058 0.0083
Golgi 0.0026 0.0003 0.0012 0.0063 0.006 0.0067 0.0023 0.0001 0.0005 0.0002 0.0027 0.001 0.0039 0.0003 0.0024 0.0002 0.0005 0.0013
Lipid Particles 0.008 0.0012 0.0068 0.0044 0.0377 0.0045 0.0077 0.001 0.0032 0.0014 0.0026 0.0031 0.0137 0.0022 0.0038 0.0061 0.0025 0.0031
Mitochondria 0.0277 0.0018 0.0105 0.0114 0.0274 0.0589 0.0268 0.0009 0.0062 0.0048 0.0471 0.0075 0.0145 0.0023 0.0056 0.0063 0.0264 0.0043
None 0.0307 0.0004 0.0063 0.0019 0.0014 0.0111 0.0142 0.0008 0.0051 0.0018 0.0259 0.004 0.0128 0.0009 0.0067 0.0023 0.0034 0.0014
Nuclear Periphery 0.0483 0.0111 0.0199 0.0148 0.0099 0.0628 0.0677 0.0051 0.0094 0.016 0.0123 0.0115 0.0275 0.0062 0.0125 0.0073 0.0038 0.0134
Nucleolus 0.0197 0.0013 0.0101 0.0212 0.005 0.0533 0.0236 0.0033 0.0036 0.0053 0.02 0.0411 0.0401 0.0023 0.0095 0.0196 0.0763 0.0037
Nucleus 0.0467 0.0081 0.0116 0.0272 0.017 0.0696 0.0389 0.0116 0.0117 0.0199 0.0221 0.014 0.0206 0.0051 0.0083 0.012 0.0165 0.0135
Peroxisomes 0.0032 0.0001 0.0023 0.0009 0.0173 0.0014 0.0016 0.0001 0.0002 0.0002 0.0006 0.0004 0.0073 0.0001 0.0036 0.0002 0.0009 0.0002
Punctate Nuclear 0.0189 0 0.0025 0.0004 0.0019 0.0039 0.0064 0.0001 0.0002 0.0001 0.0003 0.0005 0.0014 0.0001 0.0011 0.0001 0.0001 0.0003
Vacuole 0.196 0.121 0.2087 0.223 0.3083 0.0911 0.1692 0.087 0.1372 0.3347 0.14 0.1989 0.1877 0.0947 0.1706 0.1837 0.2407 0.0978
Vacuole Periphery 0.0544 0.0212 0.0358 0.0707 0.0955 0.0485 0.0348 0.0088 0.0145 0.0847 0.047 0.0718 0.0291 0.0091 0.0185 0.0457 0.0954 0.0299

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4233.2074 4459.9492 4689.122 3752.9901 3976.1852 4012.6163 3927.0862 3409.9483 3784.5883 3217.135
Translational Efficiency 0.474 0.4979 0.5066 0.5992 0.5169 0.4444 0.5078 0.555 0.4662 0.5093

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Eft2

Eft2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Eft2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available