Standard name
Human Ortholog
Description GTPase activating protein (GAP) for Rho1p; regulator of a Tor2p-mediated, Rho1p GTPase switch that controls organization of the actin cytoskeleton; negative regulator of the RHO1-PKC1-MAPK cell integrity (CWI) and membrane fluidity homeostasis signaling pathways; potential Cdc28p substrate; SAC7 has a paralog, BAG7, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.11 0.14 0.12 0.13 0.08 0.1 0.09 0.09 0.09 0.1 0.11 0.13 0.08 0.05 0.07 0.14 0.16 0.12 0.05 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.97 0.97 0.96 0.94 0.93 0.9 0.9 0.82 0.82 0.99 0.99 1.0 0.93 0.96 0.96 0.84 0.91 0.88 0.93 0.89 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0 0.18 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 3 1 2 2 5 5 1 10 12 12 11 0 0 0 0 0 0 7 6 11 1 3 8
Bud 2 3 1 1 1 8 7 11 8 5 10 4 1 0 0 0 0 0 1 0 1 1 1 0
Bud Neck 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 6 4 2 3 6
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 38 76 49 52 31 57 54 64 53 63 52 64 16 15 30 53 52 49 16 12 10 4 5 10
Cytoplasm 341 547 417 379 397 570 596 654 547 569 373 396 200 296 399 344 321 392 299 507 372 242 253 289
Endoplasmic Reticulum 6 4 1 6 6 7 11 17 17 17 9 18 1 1 0 11 9 15 9 7 8 0 3 4
Endosome 0 1 0 0 2 0 0 1 0 0 0 0 1 0 0 4 1 1 6 4 1 1 3 3
Golgi 1 1 1 0 0 0 0 1 0 0 0 0 1 0 0 2 1 1 3 0 2 0 1 2
Mitochondria 2 0 0 1 0 6 12 6 35 22 83 40 0 1 0 2 0 5 2 3 2 2 2 2
Nucleus 0 1 0 0 2 2 4 6 0 2 1 3 0 2 1 5 2 6 0 0 0 0 0 1
Nuclear Periphery 2 0 0 0 0 0 2 1 0 0 1 0 0 0 0 0 1 1 0 2 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 3 0 3 3 4 0 0 7 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 0 2 2 0 2 2 1 9 6 19 6 10 0 1 1 14 15 8 1 3 1 0 1 2
Unique Cell Count 352 560 423 390 409 592 632 704 605 631 453 482 203 299 400 369 335 408 357 560 423 260 284 339
Labelled Cell Count 395 638 475 441 447 657 695 774 680 710 547 554 220 316 431 437 402 478 357 560 423 260 284 339


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 8.4 7.9 7.4 7.9 7.4 7.7 8.2 7.7 8.2 7.3 8.0 8.2 8.0 8.2 10.4 10.8 9.6 7.6 7.9 8.5
Std Deviation (1e-4) 0.7 1.0 1.1 1.3 1.2 1.1 1.2 1.2 1.2 1.4 1.3 1.4 1.0 0.9 1.0 2.0 2.1 1.8 1.0 1.3 1.4
Intensity Change (Log2) -0.08 0.01 -0.08 -0.03 0.07 -0.04 0.07 -0.11 0.03 0.07 0.03 0.07 0.4 0.45 0.29 -0.04 0.0 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 2.4 3.0 2.7 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 2.6 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.8 -2.0 -1.0 -1.6 -1.3 -1.5 -0.8 0 0.8 -1.4 -3.1 -2.0 1.2 1.6 0.2
Cytoplasm -1.4 -1.5 -2.2 -3.5 -4.3 -5.3 -5.4 -8.1 -8.2 -0.1 0.5 1.8 -3.9 -2.4 -2.2
Endoplasmic Reticulum 0 0 0 0 2.8 3.1 3.0 0 3.7 0 0 0 3.2 0 3.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 5.0 3.9 9.3 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 2.9 0 2.1 0 0 0 3.3 3.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7029 3.3409 3.0933 2.6279 2.304 2.927 3.0617 3.5982 3.7292 3.3804 3.2434 3.4882 4.7025 5.3268 5.0397 4.2577 4.5084 4.6518
Actin 0.0314 0.0035 0.0105 0.0124 0.0178 0.0132 0.0394 0.0084 0.0226 0.0112 0.006 0.0146 0.0298 0.0082 0.0122 0.0511 0.0053 0.0097
Bud 0.003 0.0041 0.0079 0.0046 0.0175 0.0011 0.003 0.002 0.0028 0.0022 0.0005 0.0025 0.0038 0.0129 0.0065 0.0011 0.0174 0.0057
Bud Neck 0.0107 0.0011 0.001 0.0025 0.0021 0.0091 0.0113 0.0007 0.0021 0.001 0.0019 0.0092 0.0022 0.0023 0.0021 0.0009 0.0047 0.0149
Bud Periphery 0.0055 0.0021 0.0033 0.0039 0.0154 0.0047 0.004 0.0019 0.003 0.0034 0.0008 0.0093 0.0044 0.0043 0.0041 0.0012 0.0082 0.0075
Bud Site 0.0091 0.0323 0.0249 0.0227 0.007 0.0089 0.0085 0.0319 0.0319 0.0024 0.0019 0.0033 0.0119 0.042 0.0164 0.0014 0.0171 0.0016
Cell Periphery 0.0044 0.0055 0.0032 0.0008 0.0017 0.0072 0.0079 0.0046 0.004 0.0004 0.0007 0.0056 0.0059 0.0054 0.0021 0.0006 0.0008 0.0059
Cytoplasm 0.568 0.8691 0.8436 0.8562 0.8007 0.8648 0.6799 0.8917 0.7695 0.754 0.6485 0.8424 0.7704 0.8797 0.8745 0.87 0.9119 0.8614
Cytoplasmic Foci 0.0584 0.007 0.0124 0.0081 0.0208 0.0106 0.0313 0.0053 0.0247 0.0223 0.0567 0.0082 0.0145 0.0031 0.0078 0.0061 0.0063 0.0092
Eisosomes 0.0004 0.0002 0.0001 0.0002 0 0.0001 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0075 0.0039 0.0033 0.0024 0.0017 0.0026 0.0099 0.0039 0.004 0.0073 0.0041 0.0028 0.0064 0.0021 0.0042 0.002 0.0021 0.0036
Endosome 0.0498 0.0052 0.0066 0.0083 0.0104 0.0063 0.0299 0.0027 0.0212 0.0518 0.0746 0.0095 0.0253 0.0006 0.0105 0.0016 0.0107 0.0116
Golgi 0.0191 0.0012 0.0042 0.0123 0.0035 0.0035 0.0098 0.0011 0.0108 0.0204 0.0365 0.0113 0.0124 0.0005 0.0033 0.0016 0.0012 0.0067
Lipid Particles 0.0182 0.0005 0.0081 0.0019 0.0007 0.0049 0.0109 0.0005 0.0067 0.0184 0.0379 0.0025 0.0093 0.0002 0.0023 0.0002 0.0004 0.0083
Mitochondria 0.0184 0.0065 0.0125 0.0032 0.0076 0.0027 0.0081 0.0003 0.0053 0.0483 0.014 0.0088 0.0076 0.0002 0.0016 0.0002 0.0003 0.0033
None 0.0944 0.0532 0.0391 0.0541 0.0528 0.0421 0.0876 0.0416 0.0781 0.0279 0.0754 0.0537 0.0588 0.035 0.0411 0.0597 0.007 0.0344
Nuclear Periphery 0.0172 0.0005 0.0031 0.0003 0.0006 0.0028 0.0117 0.0004 0.0005 0.0032 0.0095 0.0003 0.0092 0.0005 0.0022 0.0002 0.0009 0.0073
Nucleolus 0.0033 0.0002 0.0002 0.0002 0.0011 0.0011 0.0016 0.0001 0.0006 0.0004 0.0006 0.0001 0.0016 0.0001 0.0005 0 0.0002 0.0002
Nucleus 0.0273 0.0012 0.0038 0.0007 0.0026 0.0016 0.0118 0.0008 0.0011 0.005 0.0014 0.0005 0.0064 0.0012 0.0032 0.0004 0.0022 0.0016
Peroxisomes 0.0231 0.0002 0.0096 0.0031 0.0142 0.0101 0.0168 0.0005 0.0059 0.0119 0.0187 0.0129 0.0053 0.0002 0.0013 0.0003 0.0002 0.0034
Punctate Nuclear 0.0222 0.0006 0.0008 0.0002 0.0009 0.0006 0.0066 0.0003 0.0014 0.0013 0.0014 0.0004 0.0095 0.0007 0.0016 0.0003 0.0014 0.0015
Vacuole 0.0062 0.0017 0.0016 0.0017 0.0187 0.0015 0.0085 0.0011 0.0033 0.005 0.0047 0.0015 0.0039 0.0006 0.0021 0.0011 0.0016 0.0015
Vacuole Periphery 0.0024 0.0001 0.0004 0.0003 0.0021 0.0005 0.0013 0.0001 0.0004 0.0022 0.004 0.0004 0.0011 0 0.0004 0.0001 0.0002 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.8996 25.6006 29.0539 35.1373 18.9292 32.5507 31.3408 39.3942 33.2591 31.6748
Translational Efficiency 0.7715 0.7651 0.7208 0.5275 0.5901 0.7735 0.7137 0.5248 0.6733 0.6908

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description GTPase activating protein (GAP) for Rho1p; regulator of a Tor2p-mediated, Rho1p GTPase switch that controls organization of the actin cytoskeleton; negative regulator of the RHO1-PKC1-MAPK cell integrity (CWI) and membrane fluidity homeostasis signaling pathways; potential Cdc28p substrate; SAC7 has a paralog, BAG7, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (93%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Sac7

Sac7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sac7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available